Cell Meter Fluorimetric Intracellular pH Assay Kit
货号： 21180 产品名称： Cell Meter Fluorimetric Intracellular pH Assay Kit 品牌： AAT 规格： 500 Tests 二周到货 生化试验
Cell Meter� Fluorimetric Intracellular pH Assay Kit
Cat # 21180
Storage F/D/L (see storage codes below)
Unit Size 1000 Tests
Ex (nm) 503
Em (nm) 528
Intracellular pH change are implicated in diverse physiological and pathological processes, including cell proliferation, apoptosis, fertilization, malignancy, multidrug resistance, ion transport, lysosomal storage disorders and Alzheimer抯 disease. The Cell Meter� Fluorimetric Intracellular pH Assay Kit utilizes AAT Bioquest抯 proprietary fluorescent indicator for measuring the relative intracellular pH changes. It is a homogeneous, kinetic, live-cell fluorescent assay that utilizes either a standard procedure or acid-load procedure. The standard protocol is designed for measuring the therapeutic targets of interest with a decrease in intracellular pH upon treatment. The 慉cid-Load� procedure is designed to measure the increase of intracellular pH associated with changes in cellular metabolism due to GPCR activation or growth factor activity. With the 慉cid-Load� procedure ammonium chloride solution is added after the fluorescent pH dye is loaded into cells in a minimum volume. This 慳cid-loading� step is followed by the addition of agonist in a relatively large volume (~4X) of buffer. The sudden volume change initiates an efflux of ammonia (NH3) from the cells causing a rapid decrease in intracellular pH, and thus a decrease in fluorescence signal. The effect of agonist on the subsequent recovery of intracellular pH is measured by the relative fluorescence signal increase.
Detailed Information: Protocol References MSDS Spectrum
Related Products: pH and Ion Indicators Cell Metabolism
Storage Codes:
D = Desiccated
L = Avoid light
F = Freeze (<-15 癈)
R = Refrigerated (2-4 癈)
RT = Room temperature (4-50 癈)
