Anti-Notch 1, Notch Intracellular Domain (NICD) (rabbit polyclonal)
货号： 07-1232 产品名称： Anti-Notch 1, Notch Intracellular Domain (NICD) (rabbit polyclonal) 品牌： Millipore 规格： EA 3周到货 生化实验
Anti-Notch 1 Antibody, NT
Recommended Replacement for: 07-1232 is a recommended replacement for AB5709
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REFERENCES
Regulation of notch1 signaling by nrf2: implications for tissue regeneration.
Nobunao Wakabayashi,Soona Shin,Stephen L Slocum,Elin S Agoston,Junko Wakabayashi,Mi-Kyoung Kwak,Vikas Misra,Shyam Biswal,Masayuki Yamamoto,Thomas W Kensler (2010) Science signaling.3
Species Reactivity Key Applications Host Format Antibody Type
H, M ELISA, WB, IH(P) Rabbit Serum Polyclonal Antibody
Description:
Anti-Notch 1 Antibody, NT
Replaces:
07-1232 is a recommended replacement for AB5709
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Specificity:
Notch 1, cleaved N terminal. Only the cleaved intracellular (activated) form is detected. No reactivity is detected against mouse N2, N3 or N4. This epitope is exposed only after gamma secretase cleavage and is not accessible in the uncleaved form.
Molecular Weight:
~80 kDa
Epitope:
Cleaved N-Terminus
Immunogen:
Synthetic peptide from the N-terminal sequence of the cleaved N intracellular domain (NICD) human Notch 1.
Isotype:
IgG
Background Information:
Notch is a family of single-pass transmembrane receptor proteins that are synthesized in the endoplasmic reticulum as an inactive form and are proteolytically cleaved on an extracellular site by a furin-like convertase (S1 cleavage) in the trans-golgi network after the recognition of the RQRR sequence before it reaches the plasma membrane as heterodimers to yield an active, ligand-accessible form. The Notch family is comprised of 4 members (1-4) whose ligands include the Delta and Jagged family of ligands. These ligands cause proteolysis of Notch to liberate the intracellular domain. Cleavage results in a C-terminal fragment N(TN) and a N-terminal fragment N(EC). Following ligand binding, it is cleaved (S2 cleavage) by TNF-alpha converting enzyme (TACE) to yield a membrane-associated intermediate fragment called Notch extracellular truncation (NEXT). This fragment is then cleaved by presenilin-dependent gamma-secretase between gly1743 and val1744 (S3 cleavage) to release the intracellular domain (NICD) from the membrane. That domain translocates to the nucleus and initiates transcription events by binding the DNA binding protein CSL. The notch family members are involved cell differentiation and development.
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Species Reactivity:
Human
Mouse
Species Reactivity Note:
Human and mouse. The antibody is also expected to react with rat due to immunogen sequence homology. Other species have not been tested.
Application Notes:
ELISA:
A previous lot of this antibody was diluted to 1:20,000-1:60,000 and used in a standard sandwich ELISA assay against the peptide immunogen.
Immunohistochemistry(paraffin):
Representative testing from a previous lot.
Optimal Staining of Notch-1 With Citrate pH 6.0 Epitope Retrieval: Uterine Cancer
ELISA: A previous lot of this antibody was diluted to 1:20,000-1:60,000 and used in a standard sandwich ELISA assay against the peptide immunogen.
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Control:
Jurkate Cell Lysates. In fetal tissues, use spleen, brain stem and lung. In adult tissues use mainly lymphoid tissues.
Quality Assurance:
Routinely evaluated by Western Blot on Jurkat lysates.
Western Blot Analysis: 1:500 dilution of this lot detected cleaved NOTCH1 on 10 μg of Jurkat lysates.
Presentation:
Rabbit polyclonal IgG serum in buffer containing 0.02 M Potassium Phosphate, 0.15 M NaCl, pH 7.2, containing 0.1% sodium azide.
Storage Conditions:
Stable for 6 months at -20°C in undiluted aliquots from date o -20°C
