标签归档：atpase

BML-SA113-0200   Na+/K+ ATPase, pAb   品牌 Biomol

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Na+/K+ ATPase, pAb

货号： BML-SA113-0200      产品名称： Na+/K+ ATPase, pAb   品牌： Biomol 规格： 200 ug 3周到货 生化实验

Na+/K+ ATPase, pAb
Product Specification
Alternative Name:Sodium/potassium ATPase
Host:Chicken
Immunogen:Highly purified Na+/K+ ATPase from canine kidney.
Application:ELISA
Western Blot
Specificity:Recognizes mammalian Na+/K+ ATPase.
Long Term Storage:-80°C

-80°C

506190-10MG   p97 ATPase Activity Inhibitor, DBeQ   品牌 Merck

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p97 ATPase Activity Inhibitor, DBeQ

货号： 506190-10MG      产品名称： p97 ATPase Activity Inhibitor, DBeQ   品牌： Merck 规格： *** 3周到货 生化实验

p97 ATPase Activity Inhibitor, DBeQ
CID676352, CID16472035, N2,N4-Dibenzylquinazolinine-2,4-diamine, KUC105555N, ERAD Inhibitor I, p97 Inhibitor I, Valosin-containing Protein Inhibitor I, VCP Inhibitor I
A cell-permeable quinazolinamine compound that inhibits the AAA (ATPase associated with diverse cellular activities) ATPase p97 (IC50 = 1.6 µM against WT or C522A p97) in a reversible and ATP-competitive (Ki = 3.2 µM) manner, while being at least 50-fold less potent against AAA ATPase NSF (N-methylmaleimide-sensitive factor) or the ATP-dependent chymotrypsin-like activity of proteasome 20S, and exhibiting no significant activity against a panel of ~170 kinases. Shown to effectively suppress p97-dependent cellular processes, including ERAD (ER-associated degradation) reporter TCRα-GFP degradation (92.59% vs. 33.33% degradation in 2 h, respectively, with DMSO or 10 µM DBeQ in HEK293 cultures), autophagic degradation of LC3-II (LC3-II/LC3-I = 0.04 vs. 1.0, respectively, after 3 h DMSO or 10 µM DBeQ treatment in HeLa cultures), UFD (ubiquitin fusion degradation) pathway-mediated UbG76V-GFP reporter degradation (IC50 = 2.3 µM in HeLa cells), but not the degradation of p97-independent proteasome substrates ODC (ornithine decarboxylase) and ODD (oxygen-dependent degradation domain of HIF-1α) luciferase fusion reporters (IC50 = 45 and 56 µM, respectively, in HeLa cells). Also demonstrated to display selective antiproliferative activity against RPMI8226, HeLa, and HEK293 over non-cancerous human fetal lung fibroblasts MRC5 (GI50 = 1.2, 3.1, 4.0, and 6.6 µM, respectively) by inducing cellular DEVD-like (caspase-3/7), but not VEID-like (caspase-6), activity (by 4- to 6-fold in 4 h in HeLa cells) via a yet unknown mechanism.
产品信息
Form  White solid
Molar mass  340.4
Protect from Light  是的
Packaged under inert gas  是的
Purity  ≥95% by HPLC
Solubility  DMSO or EtOH
Chemical formula  C22H20N4
商店和运输信息
Storage  -20°C
Ship  Shipped at Ambient Temperature or with Blue Ice or with Dry Ice
Regulatory Review

-20°C

BML-SA247-0100   Na+/K+ ATPase alpha3 subunit, mAb (XVIF9-G10)   品牌 Biomol

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Na+/K+ ATPase alpha3 subunit, mAb (XVIF9-G10)

货号： BML-SA247-0100      产品名称： Na+/K+ ATPase alpha3 subunit, mAb (XVIF9-G10)   品牌： Biomol 规格： 100 ug 3周到货 生化实验

Na+/K+ ATPase α3 subunit, mAb (XVIF9-G10)
Product Specification
Alternative Name:Sodium/potassium ATPase subunit α3
Clone:XVIF9-G10
Host:Mouse
Isotype:IgG1
Immunogen:Canine cardiac microsomes.
Application:Immunohistochemistry (5µg/ml)
Western Blot (1µg/ml)
Optimal conditions must be determined individually for each application.
Specificity:Recognizes human, monkey, sheep, rabbit, dog and rat Na+/K+ ATPase (α3 subunit). Detects a band of ~110 kDa in Western blot.
Purity Detail:Diluted ascites.
Formulation:Liquid. Ascites fluid containing 0.05% sodium azide.
Use/Stability:For long-term storage, aliquot the product into individual tubes and freeze at -20°C.
Handling:Avoid freeze/thaw cycles.
Long Term Storage:-20°C

-20°C

672999-10MG   V-ATPase Inhibitor, KM91104   品牌 Merck

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V-ATPase Inhibitor, KM91104

货号： 672999-10MG      产品名称： V-ATPase Inhibitor, KM91104   品牌： Merck 规格： *** 3周到货 生化实验

V-ATPase Inhibitor, KM91104
A cell-permeable, non-macrolide small molecule V-ATPase inhibitor that specifically targets the a3-b2 subunit protein protein interation of V-ATPase (IC50 of 2.3 µM), in vitro. It is also shown to inhibit osteoclast resorption (IC50 of 1.2 µM) on both synthetic hydroxyapatite surfaces and dentin slices in an in vitro osteoclast model, RANKL-differentiated RAW 264.7 cells, without significantly affecting RAW 264.7 cell viability or RANKL-mediated osteoclast differentiation.
产品信息
Form  Off-white solid
Molar mass  272.08
Protect from Light  是的
Packaged under inert gas  是的
Purity  >95% by HPLC
Solubility  DMSO
Chemical formula  C14H12N2O4
商店和运输信息
Storage  -20°C
Ship  Shipped with Blue Ice or with Dry Ice
Standard Handling

-20°C

MABN470   Anti-Copper ATPase 1, clone L60/4   品牌 Millipore

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Anti-Copper ATPase 1, clone L60/4

货号： MABN470      产品名称： Anti-Copper ATPase 1, clone L60/4   品牌： Millipore 规格： EA 三周到货 生化实验

Anti-Copper ATPase 1, clone L60/4
Species Reactivity Key Applications Host Format Antibody Type
H, R  WB, IHC Mouse Purified Monoclonal Antibody
Description:
Anti-Copper ATPase 1 Antibody, clone L60/4
Promotional Text:
Special Shipping Offer on Antibodies
100% Performance Guaranteed
Molecular Weight:
~170 kDa observed. Five isoforms are known to exist at 165 kDa, 172 kDa, 154 kDa, 54 kDa, and 12 kDa.
Immunogen:
Linear peptide corresponding to human Copper ATPase 1.
Clone:
L60/4
Isotype:
IgG2bκ
Background Information:
Encoded by the Mc1 gene, Copper ATPase 1 (a Menke’s disease protein), is a 1,500 amino acid protein, which contains six putative metal-binding motifs at the N-terminus. It is suggested to act as a P-type cation-transporting ATPase. Defects in the Mc1 gene are associated with Menke’s disease, an X-linked disorder which is characterized by improper copper transport, resulting in progressive neurological degeneration and death in early childhood. Copper ATPase 1 is expressed certain organs and cell types, but demonstrates little to know expression in liver.
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Species Reactivity:
Human
Rat
Species Reactivity Note:
Demonstrated to react with Human and Rat. Other homologies: Mouse (85% sequence homology).
Application Notes:
Immunohistochemistry Analysis: A 1:2,000 dilution of this antibody detected Copper ATPase 1 in human colorectal adenocarcinoma tissue and rat cerebellum tissue.
Immunohistochemistry Analysis: A representative lot detected Copper ATPase 1 in rat hippocampus tissue.
Western Blot Analysis: A representative lot detected Copper ATPase 1 in adult rat brain membrane tissue lysate and in COS-1 cell lysates transfected with Copper ATPase 1, but demonstrated a loss of signal in COS-1 cell lysates transfected with an empty vector.
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Control:
SK-N-SH cell lysate
Quality Assurance:
Evaluated by Western Blot in SK-N-SH cell lysate.
Western Blot Analysis: 2 µg/mL of this antibody detected Copper ATPase 1 in 10 µg of SK-N-SH cell lysate.
Purification Method:
Protein G purified
Presentation:
Purified mouse monoclonal IgG2bκ in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Storage Conditions:
Stable for 1 year at 2-8°C from date of receipt.
UniProt Number:
Q04656
Entrez Gene Number:
NP_000043
Gene Symbol:
ATP7A
MC1
MNK
Alternate Names:
Copper-transporting ATPase 1
Copper pump 1
Menkes disease-associated protein
Usage Statement:
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Key Applications:
Western Blotting
Immunohistochemistry
Product Name:
Anti-Copper ATPase 1, clone L60/4
Concentration:
Please refer to the Certificate of Analysis for the lot-specific concentration.
Antibody Type:
Monoclonal Antibody
Qty/Pk:
100 μg
Format:
Purified
Host:
Mouse
Applications:
This Copper ATPase 1 antibody is validated for use in WB & IHC for the detection of the Copper ATPase 1 protein.

2-8℃

04-255   19S Regulator ATPase Subunit S7/Rpt1,mAb   品牌 Millipore

发表于由

19S Regulator ATPase Subunit S7/Rpt1,mAb

货号： 04-255      产品名称： 19S Regulator ATPase Subunit S7/Rpt1,mAb   品牌： Millipore 规格： EA 3周到货 生化实验

Anti-19S regulator ATPase subunit S7/Rpt1 Antibody, clone MSS1-104
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REFERENCES
Simultaneous binding of PA28 and PA700 activators to 20 S proteasomes.
Hendil, K B, et al. (1998) Biochem. J., 332 ( Pt 3): 749-54 (1998)
Subunit arrangement in the human 20S proteasome
Kopp, F, et al (1997) Proc Natl Acad Sci USA, 94:2939-44 (1997)
The 26S proteasome: subunits and functions
Tanaka, K and Tsurumi, C (1997) Mol Biol Rep, 24:3-11 (1997)
Species Reactivity Key Applications Host Format Antibody Type
H, M  WB Mouse Purified Monoclonal Antibody
Description:
Anti-19S regulator ATPase subunit S7/Rpt1 Antibody, clone MSS1-104
Promotional Text:
Special Shipping Offer on Antibodies
100% Performance Guaranteed
Trade Name:
Upstate (Millipore)
Specificity:
Recognizes 19S regulator ATPase subunit S7/Rpt1.
Molecular Weight:
48 kDa
Immunogen:
Full length human recombinant Rpt1 protein.
Clone:
MSS1-104
Isotype:
IgG1
Species Reactivity:
Human
Mouse
Presentation:
100 µL concentration 1.6mg/mL of purified immunoglobulin in Tris buffer containing 0.01 M sodium azide. Dilute to working strength with phosphate buffered saline pH 7.2-7.4.
Storage Conditions:
Stable for 1 year at -20°C from date of shipment for up to 12 months.
UniProt Number:
P35998
Entrez Gene Number:
NM_002803.2
Gene Symbol:
PSMC2
MSS1
MGC3004
Nbla10058
S7
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Alternate Names:
26S proteasome ATPase subunit 2; S7
Usage Statement:
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Key Applications:
Western Blotting
Entrez Gene Summary:
The 26S proteasome is a multicatalytic proteinase complex with a highly ordered structure composed of 2 complexes, a 20S core and a 19S regulator. The 20S core is composed of 4 rings of 28 non-identical subunits; 2 rings are composed of 7 alpha subunits and 2 rings are composed of 7 beta subunits. The 19S regulator is composed of a base, which contains 6 ATPase subunits and 2 non-ATPase subunits, and a lid, which contains up to 10 non-ATPase subunits. Proteasomes are distributed throughout eukaryotic cells at a high concentration and cleave peptides in an ATP/ubiquitin-dependent process in a non-lysosomal pathway. An essential function of a modified proteasome, the immunoproteasome, is the processing of class I MHC peptides. This gene encodes one of the ATPase subunits, a member of the triple-A family of ATPases which have a chaperone-like activity. This subunit has been shown to interact with several of the basal transcription factors so, in addition to participation in proteasome functions, this subunit may participate in the regulation of transcription. This subunit may also compete with PSMC3 for binding to the HIV tat protein to regulate the interaction between the viral protein and the transcription complex.
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UniProt Summary:
FUNCTION: SwissProt: P35998 # The 26S protease is involved in the ATP-dependent degradation of ubiquitinated proteins. The regulatory (or ATPase) complex confers ATP dependency and substrate specificity to the 26S complex. In case of HIV-1 infection, positive modulator of Tat-mediated transactivation.
SIZE: 433 amino acids; 48634 Da
SUBUNIT: Interacts with KNTC2 and SQSTM1. Interacts with PAAF1 -20°C