Anti-ATM

货号： 04-200      产品名称： Anti-ATM   品牌： Millipore 规格： EA 3周到货 生化实验

Anti-ATM, clone Y170, Rabbit Monoclonal
Species Reactivity Key Applications Host Format Antibody Type
H, not M, not R  ICC, IF, IHC, IH(P), IP, WB Rabbit Culture Supernatant Monoclonal Antibody
Description:
Anti-ATM Antibody, clone Y170, rabbit monoclonal
Promotional Text:
Special Shipping Offer on Antibodies
100% Performance Guaranteed
Trade Name:
Upstate (Millipore)
Specificity:
This antibody recognizes ATM irrespective of phosphorylation on Ser1981.
Molecular Weight:
370 kDa
Epitope:
area encompassing the autophosphorylation site at Ser1981 in the FAT Domain
Immunogen:
Synthetic peptide corresponding to the FAT domain encompassing the area of Ser1981 of human ATM.
Clone:
Y170
Isotype:
IgG
Background Information:
ATM (Ataxia Telangiectasia Mutated kinase) and ATR (Ataxia Telangiectasia and Rad3-related kinase) are related kinases that regulate cell cycle checkpoints and DNA repair. ATM is activated in response to DNA damage and serves to arrest further cell division before the damage can be repaired. Mutation in the ATM gene results in the autosomal recessive disease ataxia telangiectasia (AT). The identified substrates for ATM are p53, p95/NBS1, MDM2, Chk2, BRCA1, CtIP, 4E-BP1 and Chk1. ATM activates p53, increasing p21/Cip1/Waf1 levels, thus blocking activation of Cdk2. That results in Rb hypophosphorylation and blockage of the G1/S transition. Separately, ATM also phosphorylates and activates Chk1, which phosphorylates Cdc25C. This inactivates Cdc25C and prevents it from dephosphorylating the inhibitory phosphotyrosine residue on cdc2/Cdk1, thus preventing the G2/M transition. The complex phenotype of cells derived from patients with AT suggests that ATM has additional cellular substrates. In unirradiated cells, ATM is present as an inactive homodimer or multimer. Double-stranded breaks in DNA caused by ionizing radiation cause rapid ATM kinase activation through dissociation of this complex and ATM autophosphorylation at Ser1981.
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Species Reactivity:
Human
Species Reactivity Note:
Proven to react with human. Not expected to react with mouse or rat.
Non-Reactive Species:
Mouse
Rat
Application Notes:
Immunocytochemistry:
A 1:100-250 dilution of a previous lot of this antibody was used on immunocytochemistry.
Immunohistochemistry:
A 1:250 dilution of a previous lot of this antibody was used on immunohistochemistry.
Immunoprecipitation:
A 1:50 dilution of a previous lot of this antibody was used on immunoprecipitation.
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Control:
HEK293 cell lysate.
Quality Assurance:
Routinely evaluated by Western Blot on HEK293 cell lysate.
Western Blot Analysis:
A 1:2,500-1:5,000 dilution of this lot was used to detect ATM in HEK293 cell lysate.
Purification Method:
Unpurified
Presentation:
Unpurified rabbit monoclonal IgG in buffer containing 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA.
Storage Conditions:
Stable for 1 year at -20ºC from date of receipt.
Handling Recommendations: Upon receipt, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance. Note: Variability in freezer temperatures below -20°C may cause glycerol containing solutions to become frozen during storage.
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UniProt Number:
Q13315
Entrez Gene Number:
NM_000051.3
Gene Symbol:
AT1
ATA
ATC
ATD
ATDC
ATE
DKFZp781A0353 -20°C

Anti-ATM

货号： 07-1286      产品名称： Anti-ATM   品牌： Millipore 规格： EA 3周到货 生化实验

Anti-ATM
Species Reactivity Key Applications Host Format Antibody Type
H, M, R, Eq, B  WB, IF Rabbit Affinity Purified Polyclonal Antibody
Description:
Anti-ATM Antibody
Promotional Text:
Special Shipping Offer on Antibodies
100% Performance Guaranteed
Trade Name:
Upstate (Millipore)
Specificity:
Detects ATM.
Molecular Weight:
350 kDa
Epitope:
Central
Immunogen:
KLH-conjugated synthetic peptide containing amino acids of internal human ATM in the Abl interacting domain.
Isotype:
IgG
Background Information:
ATM (Ataxia Telangiectasia Mutated kinase) and ATR (Ataxia Telangiectasia and Rad3-related kinase) are related kinases that regulate cell cycle checkpoints and DNA repair. ATM is activated in response to DNA damage and serves to arrest further cell division before the damage can be repaired. Mutation in the ATM gene results in the autosomal recessive disease ataxia telangiectasia (AT). The identified substrates for ATM include p53, p95/NBS1, MDM2, Chk2, BRCA1, CtIP, 4E-BP1 and Chk1. ATM activates p53, increasing p21/Cip1/Waf1 levels, thus blocking activation of Cdk2. That results in Rb hypophosphorylation and blockage of the G1/S transition. Separately, ATM also phosphorylates and activates Chk1, which phosphorylates Cdc25C. This inactivates Cdc25C and prevents it from dephosphorylating the inhibitory phosphotyrosine residue on cdc2/Cdk1, thus preventing the G2/M transition. The complex phenotype of cells derived from patients with AT suggests that ATM has additional cellular substrates. In unirradiated cells, ATM is present as an inactive homodimer or multimer. Double-stranded breaks in DNA caused by ionizing radiation cause rapid ATM kinase activation through dissociation of this complex and ATM autophosphorylation at Ser1981.
Species Reactivity:
Human
Mouse
Rat
Horse
Bovine
Species Reactivity Note:
Human, Mouse, and Rat. Expected to react with horse and bovine based on 100% sequence homology.
Application Notes:
Immunofluorescence Confocal Microscopy Analysis:
HeLa cells (A & B) and NIH/3T3 cells (C & D) were fixed, permeabilized, and stained using Anti-ATM (Cy3, Red). Cells were co-stained for actin (Phalloidin, AlexaFluor® 488 conjugate, Green) and for panels B & D, staining includes nuclear staining with DAPI (Blue).
Control:
HeLa nuclear extract
Quality Assurance:
Routinely evaluated by western blot.
Western Blot Analysis:
This lot detected ATM at 1:500 dilution in HeLa nuclear extract lysates via SDS-PAGE and transferred to PVDF (Immobilon-P).
Purification Method:
Antigen affinity purified
Presentation:
Antigen Affinity purified rabbit serum in 0.1M Tris-Glycine (pH7.4) 15mM NaCl, and 0.05% NaN3.
Storage Conditions:
Stable for 1 year at 2-8°C from date of receipt.
Handling Recommendations: Upon receipt, and prior to removing the cap, centrifuge the vial and gently mix the solution.
UniProt Number:
Q13315
Entrez Gene Number:
NP_000042.
Gene Symbol:
AT1
ATA
ATC
ATD
ATDC
ATE
DKFZp781A0353 2
EC 2.7.11.1 3
MGC74674
TEL1
TELO1
Alternate Names:
ataxia telangiectasia mutated
A-T, mutated
AT mutated
TEL1, telomere maintenance 1, homolog
ataxia telangiectasia mutated (includes complementation groups A, C and D)
ataxia telangiectasia mutated protein
human phosphatidylinositol 3-kinase homolog
serine-protein kinase ATM
Usage Statement:
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use 2°C – 8°C