标签归档：atf

04-068   anti- ATF1   品牌 Millipore

发表于由

anti- ATF1

货号： 04-068      产品名称： anti- ATF1   品牌： Millipore 规格： EA 3周到货 生化实验

Anti-ATF1 Antibody, rabbit monoclonal
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REFERENCES
Leptin enhances, via AP-1, expression of aromatase in the MCF-7 cell line.
Catalano, Stefania, et al. (2003) J. Biol. Chem., 278: 28668-76 (2003)
Association of the t(12;22)(q13;q12) EWS/ATF1 rearrangement with polyphenotypic round cell sarcoma of bone: a case report
Somers, Gino R, et al (2005) Am J Surg Pathol, 29:1673-9 (2005)
Induction of tissue factor expression in human monocytic cells by protease inhibitors through activating activator protein-1 (AP-1) with phosphorylation of Jun-N-terminal kinase and p38
Ohsawa, Mai, et al (2003) Thromb Res, 112:313-20 (2003)
Vasoactive intestinal peptide upregulates MUC2 intestinal mucin via CREB/ATF1
Hokari, Ryota, et al (2005) Am J Physiol Gastrointest Liver Physiol, 289:G949-59 (2005)
Species Reactivity Key Applications Host Format Antibody Type
H  WB Rabbit Culture Supernatant Monoclonal Antibody
Description:
Anti-ATF1 Antibody, rabbit monoclonal
Promotional Text:
Special Shipping Offer on Antibodies
100% Performance Guaranteed
Trade Name:
Upstate (Millipore)
Specificity:
ATF1
Molecular Weight:
37 kDa
Immunogen:
KLH-conjugated synthetic peptide CPVTLTSQTTKTDDPQL
Isotype:
IgG
Species Reactivity:
Human
Application Notes:
Suggested dilution:
Western Blot: 1:1,000
Quality Assurance:
Routinely evaluated by immunoblot.
Presentation:
100 μL of rabbit monoclonal IgG in 60% storage buffer (50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 0.01% sodium azide and 0.05% BSA) and 40% glycerol.
Storage Conditions:
2 years at -20°C from date of shipment
UniProt Number:
P18846
Entrez Gene Number:
NM_005171.3
Gene Symbol:
ATF1
EWS-ATF1
TREB36
FUS/ATF-1
Usage Statement:
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Key Applications:
Western Blotting
UniProt Summary:
FUNCTION: SwissProt: P18846 # This protein binds the cAMP response element (CRE) (consensus: 5′-GTGACGT[AC][AG]-3′), a sequence present in many viral and cellular promoters. Binds to the Tax-responsive element (TRE) of HTLV-I. Mediates PKA-induced stimulation of CRE-reporter genes.
SIZE: 271 amino acids; 29233 Da
SUBUNIT: Binds DNA as a dimer.
SUBCELLULAR LOCATION: Nucleus.
SIMILARITY: SwissProt: P18846 ## Belongs to the bZIP family. ATF subfamily. & Contains 1 bZIP domain. & Contains 1 KID (kinase-inducible) domain.
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Brand Family:
Upstate
Product Name:
Anti-ATF1 Antibody, rabbit monoclonal
Antibody Type:
Monoclonal Antibody
Qty/Pk:
100 µL
Format:
Culture Supernatant
Host:
Rabbit
Applications:
This Anti-ATF1 Antibody, Rabbit is validated for use in WB for the detection of ATF1.

-20°C

04-1021   Anti-ATF2 (C-term), clone E243   品牌 Millipore

发表于由

Anti-ATF2 (C-term), clone E243

货号： 04-1021      产品名称： Anti-ATF2 (C-term), clone E243   品牌： Millipore 规格： EA 3周到货 生化实验

Anti-ATF-2 (C-term), clone E243, Rabbit Monoclonal
Species Reactivity Key Applications Host Format Antibody Type
H, M  WB, ICC, IH(P), IP Rabbit Unpurified Monoclonal Antibody
Description:
Anti-ATF-2 (CT) Antibody, clone E243, rabbit monoclonal
Promotional Text:
Special Shipping Offer on Antibodies
100% Performance Guaranteed
Specificity:
The antibody does not cross-react with other ATF family members.
Molecular Weight:
~70 kDa
Epitope:
C-terminus
Immunogen:
Synthetic peptide corresponding to residues in C-terminus of human ATF-2.
Clone:
E243
Isotype:
IgG
Background Information:
The ATF/CREB family of DNA binding proteins consists of transcription factors whose activity is regulated by specific signaling pathways, including the stress/cytokine/radiation inducible pathways that function through SAPK1/JNK activation. Two threonine residues at positions 69 and 71 in the N-terminal region of ATF-2 are essential in mediating adenovirus E1A-inducible transcriptional activation. These residues are efficiently phosphorylated in vivo and are excellent substrates for SAPK1/JNK in vitro.
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Species Reactivity:
Human
Mouse
Species Reactivity Note:
Human and mouse.
Application Notes:
Immunohistochemistry Analysis: A 1:250-500 dilution from a previous lot detected ATF-2 in human breast carcinoma tissue.
Immunocytochemistry Analysis: A 1:250-500 dilution from a previous lot was used in IC.
Immunoprecipitation Analysis: A 1:60 dilution from a previous lot was used in IP.
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Control:
HeLa cell lysate
Quality Assurance:
Evaluated by Western Blot on HeLa cell lysates.
Western Blot Analysis: A 1:10,000 dilution of this antibody was used to detect ATF-2 in HeLa cell lysate.
Purification Method:
Unpurified
Presentation:
Rabbit Monoclonal in buffer containing 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl containing 40% Glycerol, 0.01% sodium azide and 0.05% BSA.
Storage Conditions:
Stable for 1 year at -20ºC from date of receipt. Handling Recommendations: Upon first thaw, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance. Note: Variability in freezer temperatures below -20°C may cause glycerol containing solutions to become frozen during storage.
View All »
UniProt Number:
P15336
Entrez Gene Number:
NM_001880
Gene Symbol:
CREB-2
CREB2
CREBP1
HB16
MGC111558
OTTHUMP00000163262
TREB7
CRE-BP1
View All »
Alternate Names:
Cyclic AMP-responsive element-binding protein 2
activating transcription factor 2
activating transcription factor 2 splice variant ATF2-var2
cAMP response element-binding protein CRE-BP1
cAMP responsive element binding protein 2
cAMP responsive element binding protein 2, formerly
cAMP-dependent transcription factor ATF-2
cAMP-responsive element-binding protein 2
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Usage Statement:
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
View All »
Key Applications:
Western Blotting
Immunocytochemistry
Immunohistochemistr -20°C

04-199   Anti-phospho-ATF2 (Thr71)   品牌 Millipore

发表于由

Anti-phospho-ATF2 (Thr71)

货号： 04-199      产品名称： Anti-phospho-ATF2 (Thr71)   品牌： Millipore 规格： EA 3周到货 生化实验

Anti-phospho-ATF-2 (Thr71), clone E268, Rabbit Monoclonal
Species Reactivity Key Applications Host Format Antibody Type
H, not M, not R  IP, WB, ICC Rabbit Ascites Monoclonal Antibody
Description:
Anti-phospho-ATF-2 (Thr71) Antibody, clone E268, rabbit monoclonal
Promotional Text:
Special Shipping Offer on Antibodies
100% Performance Guaranteed
Trade Name:
Upstate (Millipore)
Specificity:
This antibody recognizes phosphorylated residues surrounding Thr71 of human ATF-2.
Molecular Weight:
70 kDa
Epitope:
Thr71
Immunogen:
Synthetic peptide directed against the region encompassing and including phosphorylated ATF2 (Thr71).
Modifications:
Phosphorylation
Clone:
E268
Isotype:
IgG
Background Information:
The ATF/CREB family of DNA binding proteins consists of transcription factors whose activity is regulated by specific signaling pathways, including the stress/cytokine/radiation inducible pathways that function through SAPK1/JNK activation. Two threonine residues at positions 69 and 71 in the N-terminal region of ATF-2 are essential in mediating adenovirus E1A-inducible transcriptional activation. These residues are efficiently phosphorylated in vivo and are excellent substrates for SAPK1/JNK in vitro.
View All »
Species Reactivity:
Human
Species Reactivity Note:
Proven to react with Human. Not expected to react with rat and mouse
Non-Reactive Species:
Mouse
Rat
Application Notes:
Immunofluorescent Immunocytochemistry:
Immunoprecipitation:
A previous lot of this antibody was used in IP.
Control:
Untreated and Calyculin/Okadaic acid treated HeLa Cell Lysate.
Quality Assurance:
Routinely evaluated by Western Blot on untreated and Anisomycin treated HeLa cell lysate.
Western Blot Analysis:
A 1:2500-1:5000 dilution of this lot was used to detect ATF-2 in untreated and Anisomycin treated HeLa cell lysate.
View All »
Purification Method:
Unpurified
Presentation:
Unpurified rabbit monoclonal IgG in buffer containing 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA.
Storage Conditions:
Stable for 1 year at -20ºC from date of receipt.
Handling Recommendations: Upon receipt, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance. Note: Variability in freezer temperatures below -20°C may cause glycerolcontaining solutions to become frozen during storage.
View All »
UniProt Number:
P15336
Entrez Gene Number:
NM_001880.2
Gene Symbol:
CRE-BP1
CREB2
CREBP1
HB16
MGC111558
OTTHUMP00000163262
TREB7
View All »
Alternate Names:
activating transcription factor 2
activating transcription factor 2 splice variant ATF2-var2
cAMP response element-binding protein CRE-BP1
cAMP responsive element binding protein 2
cAMP responsive element binding protein 2, formerly
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Usage Statement:
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
View All »
Key Applications:
Immunoprecipitation
Western Bl 2°C – 8°C

05-891   a-ph-ATF2(Thr69/71),cl AW65 200ug   品牌 Millipore

发表于由

a-ph-ATF2(Thr69/71),cl AW65 200ug

货号： 05-891      产品名称： a-ph-ATF2(Thr69/71),cl AW65 200ug   品牌： Millipore 规格： EA 3周到货 生化实验

Anti-phospho-ATF2 (Thr69/71) Antibody, clone AW65
Close
REFERENCES
Growth factor stimulation induces cell survival by c-Jun. ATF2-dependent activation of Bcl-XL.
Ahmad Salameh,Federico Galvagni,Francesca Anselmi,Caterina De Clemente,Maurizio Orlandini,Salvatore Oliviero (2010) The Journal of biological chemistry.285
Species Reactivity Key Applications Host Format Antibody Type
H, M  IP, WB Mouse Purified Monoclonal Antibody
Description:
Anti-phospho-ATF2 (Thr69/71) Antibody, clone AW65
Promotional Text:
Special Shipping Offer on Antibodies
100% Performance Guaranteed
Trade Name:
Upstate (Millipore)
Specificity:
ATF2 phosphorylated at both Thr69 and Thr71
Molecular Weight:
Mr 65kDa
Immunogen:
KLH-conjugated, synthetic peptide containing the sequence …DQ[pT]P[pT]P… in which [pT] corresponds to phospho-threonine at residues 69 and 71 of human ATF2
Modifications:
Phosphorylation
Clone:
AW65
Isotype:
IgG1
Species Reactivity:
Human
Mouse
Species Reactivity Note:
Predicted to cross-react with rat based on sequence homology
Quality Assurance:
routinely evaluated by immunoblot with RIPA lysates from anisomycin treated 3T3/NIH cells
Purification Method:
Protein G purfied
Storage Conditions:
stable 2 years at -20°C from date of shipment
UniProt Number:
P15336
Entrez Gene Number:
NM_001880.2
Gene Symbol:
ATF2
MGC111558
CREB2
TREB7
CRE-BP1
CREBP1
HB16
View All »
Usage Statement:
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
View All »
Key Applications:
Immunoprecipitation
Western Blotting
Entrez Gene Summary:
This gene encodes a transcription factor that is a member of the leucine zipper family of DNA binding proteins. This protein binds to the cAMP-responsive element (CRE), an octameric palindrome. The protein forms a homodimer or heterodimer with c-Jun and stimulates CRE-dependent transcription. The protein is also a histone acetyltransferase (HAT) that specifically acetylates histones H2B and H4 in vitro; thus it may represent a class of sequence-specific factors that activate transcription by direct effects on chromatin components. Additional transcript variants have been identified but their biological validity has not been determined.
View All »
UniProt Summary:
FUNCTION: SwissProt: P15336 # Transcriptional activator, probably constitutive, which binds to the cAMP-responsive element (CRE) (consensus: 5′- GTGACGT[AC][AG]-3′), a sequence present in many viral and cellular promoters. Interaction with JUN redirects JUN to bind to CRES preferentially over the 12-O-tetradecanoylphorbol-13-acetate response elements (TRES) as part of an ATF2-c-Jun complex.
SIZE: 487 amino acids; 52277 Da
SUBUNIT: Binds DNA as a dimer and can form a homodimer in the absence of DNA. Can form a heterodimer with JUN. Interacts with SMAD3 and SMAD4. Binds through its N-terminal region to UTF1 which acts as a coactivator of ATF2 transcriptional activity.
SUBCELLULAR LOCATION: Nucleus.
TISSUE SPECIFICITY: Abundant expression seen in the brain.
PTM: Phosphorylation of Thr-51 and Thr-53 by MAPK14 causes increased transcriptional activity. Also phosphorylated and activated by JNK.
SIMILARITY: SwissProt: P15336 # -20°C

05-891SP   a-ph-ATF2(Thr69/71),cl AW65   品牌 Millipore

发表于由

a-ph-ATF2(Thr69/71),cl AW65

货号： 05-891SP      产品名称： a-ph-ATF2(Thr69/71),cl AW65   品牌： Millipore 规格： EA 三周到货 生化实验

Anti-phospho-ATF2 (Thr69/71) Antibody, clone AW65
Close
REFERENCES
Growth factor stimulation induces cell survival by c-Jun. ATF2-dependent activation of Bcl-XL.
Ahmad Salameh,Federico Galvagni,Francesca Anselmi,Caterina De Clemente,Maurizio Orlandini,Salvatore Oliviero (2010) The Journal of biological chemistry.285
Species Reactivity Key Applications Host Format Antibody Type
H, M  IP, WB Mouse Purified Monoclonal Antibody
Description:
Anti-phospho-ATF2 (Thr69/71) Antibody, clone AW65
Promotional Text:
Special Shipping Offer on Antibodies
100% Performance Guaranteed
Trade Name:
Upstate (Millipore)
Specificity:
ATF2 phosphorylated at both Thr69 and Thr71
Molecular Weight:
Mr 65kDa
Immunogen:
KLH-conjugated, synthetic peptide containing the sequence …DQ[pT]P[pT]P… in which [pT] corresponds to phospho-threonine at residues 69 and 71 of human ATF2
Modifications:
Phosphorylation
Clone:
AW65
Isotype:
IgG1
Species Reactivity:
Human
Mouse
Species Reactivity Note:
Predicted to cross-react with rat based on sequence homology
Quality Assurance:
routinely evaluated by immunoblot with RIPA lysates from anisomycin treated 3T3/NIH cells
Purification Method:
Protein G purfied
Storage Conditions:
stable 2 years at -20°C from date of shipment
UniProt Number:
P15336
Entrez Gene Number:
NM_001880.2
Gene Symbol:
ATF2
MGC111558
CREB2
TREB7
CRE-BP1
CREBP1
HB16
View All »
Usage Statement:
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
View All »
Key Applications:
Immunoprecipitation
Western Blotting
Entrez Gene Summary:
This gene encodes a transcription factor that is a member of the leucine zipper family of DNA binding proteins. This protein binds to the cAMP-responsive element (CRE), an octameric palindrome. The protein forms a homodimer or heterodimer with c-Jun and stimulates CRE-dependent transcription. The protein is also a histone acetyltransferase (HAT) that specifically acetylates histones H2B and H4 in vitro; thus it may represent a class of sequence-specific factors that activate transcription by direct effects on chromatin components. Additional transcript variants have been identified but their biological validity has not been determined.
View All »
UniProt Summary:
FUNCTION: SwissProt: P15336 # Transcriptional activator, probably constitutive, which binds to the cAMP-responsive element (CRE) (consensus: 5′- GTGACGT[AC][AG]-3′), a sequence present in many viral and cellular promoters. Interaction with JUN redirects JUN to bind to CRES preferentially over the 12-O-tetradecanoylphorbol-13-acetate response elements (TRES) as part of an ATF2-c-Jun complex.
SIZE: 487 amino acids; 52277 Da
SUBUNIT: Binds DNA as a dimer and can form a homodimer in the absence of DNA. Can form a heterodimer with JUN. Interacts with SMAD3 and SMAD4. Binds through its N-terminal region to UTF1 which acts as a coactivator of ATF2 transcriptional activity.
SUBCELLULAR LOCATION: Nucleus.
TISSUE SPECIFICITY: Abundant expression seen in the brain.
PTM: Phosphorylation of Thr-51 and Thr-53 by MAPK14 causes increased transcriptional activity. Also phosphorylated and activated by JNK.
SIMILARITY: SwissProt: P15336 # -20℃