V13241   |ALEXA FLUOR 488 ANNEXIN V/DEAD|双染色流式法细胞凋亡检测试剂盒|Annexin V and PI   品牌 Invitrogen

|ALEXA FLUOR 488 ANNEXIN V/DEAD|双染色流式法细胞凋亡检测试剂盒|Annexin V and PI

货号: V13241      产品名称: |ALEXA FLUOR 488 ANNEXIN V/DEAD|双染色流式法细胞凋亡检测试剂盒|Annexin V and PI   品牌: Invitrogen 规格: *** 现货

Dead Cell Apoptosis Kit with Annexin V Alexa Fluor 488 & PI – for Flow Cytometry (Replaces PHN1008, PHN1010)
Vybrant Apoptosis Assay Kit #2 检测凋亡细胞中磷脂酰丝氨酸的外化。 该试剂盒利用了我们的明亮绿色荧光 Alexa Fluor 488 annexin 和红色荧光碘化丙啶核酸染料的即用型溶液。 碘化丙啶使坏死细胞着色,发出红色荧光。 在用两种探针处理之后,凋亡细胞显示绿色荧光,死细胞显示红色和绿色荧光,活细胞显示很弱的荧光或不显示荧光。
Regulatory Statement: For Research Use Only. Not for any animal or human therapeutic or diagnostic use.
Format: Tube(s),
Slide(s)
Platform: Flow Cytometer,
Fluorescence Microscope
Label or Dye: Propidium iodide,
Alexa Fluor® 488
Product Size: 50 assays
Detection Method: Fluorescent

ABC151   Anti-Annexin A1   品牌 Millipore

Anti-Annexin A1

货号: ABC151      产品名称: Anti-Annexin A1   品牌: Millipore 规格: EA 三周到货 生化实验

Anti-Annexin A1
Species Reactivity Key Applications Host Format Antibody Type
H, M, R, Porcine, Chp, B  WB, IHC Rabbit Purified Polyclonal Antibody
Description:
Anti-Annexin A1, Antibody
Promotional Text:
Special Shipping Offer on Antibodies
100% Performance Guaranteed
Specificity:
This antibody recognizes the C-terminus of Annexin A1
Molecular Weight:
~38 kDa observed
Epitope:
C-terminus
Immunogen:
KLH-conjugated linear peptide corresponding to the C-terminus of human Annexin A1.
Background Information:
Annexin A1 (ANXA1) is a calcium and phospholipid binding protein almost entirely restricted to microglia in the CNS. During the peripheral inflammatory response, Annexin A1 is instrumental in the phagocytosis of apoptotic leukocytes. Annexin A1 restores the ability of microglia to differentiate between apoptotic and non-apoptotic cells during inflammatory activation. Current research is geared towards using Annexin A1 as a therapeutic target in neuroinflammation. Annexin A1 has also been shown to be influential in cancer development and proliferation.
View All »
Species Reactivity:
Human
Mouse
Rat
Porcine
Chimpanzee
Bovine
View All »
Species Reactivity Note:
Demonstrated to react with Human, Mouse, and Rat. Predicted to react with Porcine, Chimpanzee, and Bovine based on 100% sequence homology.
Application Notes:
Western Blot Analysis: 2.0 µg/mL from a representative lot detected Annexin A1 in 10 µg of C2C12, HUVEC, and NIH/3T3 cell lysate.
Immunohistochemistry Analysis: 1:500 dilution from a representative lot detected Annexin A1 in human lung tissue.
Control:
L6 cell lysate
Quality Assurance:
Evaluated by Western Blot in L6 cell lysate.
Western Blot Analysis: 2.0 µg/mL of this antibody detected Annexin A1 in 10 µg of L6 cell lysate.
Purification Method:
Protein A purified
Presentation:
Purified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Storage Conditions:
Stable for 1 year at 2-8°C from date of receipt.
UniProt Number:
P04083
Entrez Gene Number:
NP_000691
Gene Symbol:
ANXA1
ANX1
LPC1
Alternate Names:
Annexin A1
Annexin I
Annexin-1
Calpactin II
Calpactin-2
Chromobindin-9
Lipocortin I
Phospholipase A2 inhibitory protein
p35
View All »
Usage Statement:
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
View All »
Key Applications:
Western Blotting
Immunohistochemistry
Product Name:
Anti-Annexin A1
Concentration:
Please refer to the Certificate of Analysis for the lot-specific concentration.
Antibody Type:
Polyclonal Antibody
Qty/Pk:
100 μg
Format:
Purified
Host:
Rabbit
Applications:
Anti-Annexin A1 is an antibody targeting the Annexin A1 protein, validated for use in WB & IHC.

2-8℃

ALX-201-116-C100   Annexin V (human) (recombinant)   品牌 Alexis

Annexin V (human) (recombinant)

货号: ALX-201-116-C100      产品名称: Annexin V (human) (recombinant)   品牌: Alexis 规格: 100.micro.g 三周 生化实验

Annexin V (human), (recombinant) (GST-tag)
Bulk Request
Product Specification
MW: ~52kDa (SDS-PAGE).
Source: Produced in E. coli. Recombinant human annexin V is fused to a GST-tag.
UniProt ID: P08758
Formulation: Lyophilized (100μg/aliquot). In PBS, pH 7.4.
Purity: ≥95% (SDS-PAGE)
Purity Detail: Affinity purified.
Specificity: Binds to phosphatidylserine (PS).
Biological Activity: Exhibits anti-phospholipase activity.
Reconstitution: Reconstitute with 100µl sterile water.
Long Term Storage: -20°C
Use/Stability: Reconstituted solutions can be kept at 4°C under sterile conditions for one week or should be frozen in aliquots at -20°C. Repeated freeze/thaw cycles (>3) may result in an adverse effect on the activity of Annexin V.

-20°C

ALX-209-002-T300   Annexin V (human) (recombinant) (Biotin)   品牌 Alexis

Annexin V (human) (recombinant) (Biotin)

货号: ALX-209-002-T300      产品名称: Annexin V (human) (recombinant) (Biotin)   品牌: Alexis 规格: 300 tests 三周 生化实验

Annexin V (human), (recombinant) (biotin conjugate) 
Product Specification
MW: ~35.8kDa.
Source: Produced in E. coli.
UniProt ID: P08758
Quantity: 20 tests/100µl; 100 tests/500µl; 300 tests/1’500µl.
Formulation: Liquid. In 50mM TRIS, pH 7.4, containing 100mM sodium chloride, 1% BSA and 0.02% sodium azide.
Purity: ≥98% (SDS-PAGE, HPLC)
Specificity: Binds to phosphatidylserine (PS).
Biological Activity: Exhibits anti-phospolipase activity.
Application: Detection of apoptotic cells by flow cytometry.
Long Term Storage: +4°C
Handling: Do not freeze.

+4°C

ALX-209-250-T020   Annexin V (human) (recombinant) (FITC)   品牌 Alexis

Annexin V (human) (recombinant) (FITC)

货号: ALX-209-250-T020      产品名称: Annexin V (human) (recombinant) (FITC)   品牌: Alexis 规格: 20 tests 三周 生化实验

Annexin V (human), (recombinant) (FITC conjugate)
FITC-labelled recombinant human annexin V shows bright green fluorescence (Ex(max): 488nm; Em(max): 530nm).
Product Specification
MW: ~35.8kDa.
Source: Produced in E. coli.
UniProt ID: P08758
Quantity: 20 tests/100µl; 100 tests/500µl; 300 tests/1’500µl.
Formulation: Liquid. In 50mM TRIS, pH 7.4, containing 100mM sodium chloride, 1% BSA and 0.02% sodium azide.
Purity: ≥98% (SDS-PAGE, HPLC)
Specificity: Binds to phosphatidylserine (PS).
Biological Activity: Exhibits anti-phospholipase activity.
Application: Detection of apoptotic cells by flow cytometry.
Long Term Storage: +4°C
Handling: After opening, prepare aliquots and store at -20°C.

+4°C

ALX-209-250-T100   Annexin V (human) (recombinant) (FITC)   品牌 Alexis

Annexin V (human) (recombinant) (FITC)

货号: ALX-209-250-T100      产品名称: Annexin V (human) (recombinant) (FITC)   品牌: Alexis 规格: 100 tests 三周 生化实验

Annexin V (human), (recombinant) (FITC conjugate)
FITC-labelled recombinant human annexin V shows bright green fluorescence (Ex(max): 488nm; Em(max): 530nm).
Product Specification
MW: ~35.8kDa.
Source: Produced in E. coli.
UniProt ID: P08758
Quantity: 20 tests/100µl; 100 tests/500µl; 300 tests/1’500µl.
Formulation: Liquid. In 50mM TRIS, pH 7.4, containing 100mM sodium chloride, 1% BSA and 0.02% sodium azide.
Purity: ≥98% (SDS-PAGE, HPLC)
Specificity: Binds to phosphatidylserine (PS).
Biological Activity: Exhibits anti-phospholipase activity.
Application: Detection of apoptotic cells by flow cytometry.
Long Term Storage: +4°C
Handling: After opening, prepare aliquots and store at -20°C.

+4°C

ALX-209-250-T300   Annexin V (human) (recombinant) (FITC)   品牌 Alexis

Annexin V (human) (recombinant) (FITC)

货号: ALX-209-250-T300      产品名称: Annexin V (human) (recombinant) (FITC)   品牌: Alexis 规格: 300 tests 三周 生化实验

Annexin V (human), (recombinant) (FITC conjugate)
FITC-labelled recombinant human annexin V shows bright green fluorescence (Ex(max): 488nm; Em(max): 530nm).
Product Specification
MW: ~35.8kDa.
Source: Produced in E. coli.
UniProt ID: P08758
Quantity: 20 tests/100µl; 100 tests/500µl; 300 tests/1’500µl.
Formulation: Liquid. In 50mM TRIS, pH 7.4, containing 100mM sodium chloride, 1% BSA and 0.02% sodium azide.
Purity: ≥98% (SDS-PAGE, HPLC)
Specificity: Binds to phosphatidylserine (PS).
Biological Activity: Exhibits anti-phospholipase activity.
Application: Detection of apoptotic cells by flow cytometry.
Long Term Storage: +4°C
Handling: After opening, prepare aliquots and store at -20°C.

+4°C

ALX-209-251-T020   Annexin V (human) (recombinant) (R-PE)   品牌 Alexis

Annexin V (human) (recombinant) (R-PE)

货号: ALX-209-251-T020      产品名称: Annexin V (human) (recombinant) (R-PE)   品牌: Alexis 规格: 20 tests 三周 生化实验

Annexin V (human), (recombinant) (R-PE conjugate)
R-PE-labelled recombinant human annexin V is suitable for dual staining experiments to detect phosphatidylserine whilst cell-surface protein can be detected with a suitable MAb (FITC-labelled or labelled secondary antibody). The fluorochrome R-PE (phycoerythrin) shows red fluorescence (Ex(max): 495-564nm; Em(max): 576nm). Exhibits anti-phospholipase activity.
Product Specification
MW: ~35.8kDa.
Source: Produced in E. coli.
UniProt ID: P08758
Quantity: 20 tests/100µl; 100 tests/500µl.
Formulation: Liquid. In 50mM TRIS, pH 7.4, containing 100mM sodium chloride, 1% BSA and 0.02% sodium azide.
Purity: ≥98% (SDS-PAGE, HPLC)
Specificity: Binds to phosphatidylserine (PS).
Application: Detection of apoptotic cells by flow cytometry.
Long Term Storage: +4°C
Handling: Protect from light. Do not freeze.

+4°C

ALX-209-251-T100   Annexin V (human) (recombinant) (R-PE)   品牌 Alexis

Annexin V (human) (recombinant) (R-PE)

货号: ALX-209-251-T100      产品名称: Annexin V (human) (recombinant) (R-PE)   品牌: Alexis 规格: 100 tests 三周 生化实验

Annexin V (human), (recombinant) (R-PE conjugate) 
R-PE-labelled recombinant human annexin V is suitable for dual staining experiments to detect phosphatidylserine whilst cell-surface protein can be detected with a suitable MAb (FITC-labelled or labelled secondary antibody). The fluorochrome R-PE (phycoerythrin) shows red fluorescence (Ex(max): 495-564nm; Em(max): 576nm). Exhibits anti-phospholipase activity.
Product Specification
MW: ~35.8kDa.
Source: Produced in E. coli.
UniProt ID: P08758
Quantity: 20 tests/100µl; 100 tests/500µl.
Formulation: Liquid. In 50mM TRIS, pH 7.4, containing 100mM sodium chloride, 1% BSA and 0.02% sodium azide.
Purity: ≥98% (SDS-PAGE, HPLC)
Specificity: Binds to phosphatidylserine (PS).
Application: Detection of apoptotic cells by flow cytometry.
Long Term Storage: +4°C
Handling: Protect from light. Do not freeze.

+4°C