L1110   S30 T7 High-Yield Protein Expression System   品牌 Promega

S30 T7 High-Yield Protein Expression System

货号: L1110      产品名称: S30 T7 High-Yield Protein Expression System   品牌: Promega 规格: 24 reactions 二周到货 生化试验

说明  
 E. coli S30 T7 High-Yield Protein Expression System(E. coli S30 T7高产率蛋白表达系统)被设计用于在1小时内表达高达500μg/ml的蛋白,本系统使用的载体为带有T7启动子和核糖体结合位点的质粒载体。本系统提供的提取物含有用于转录的T7 RNA聚合酶,并使OmpT内源蛋白酶和Ion蛋白酶活性缺失。系统内所有其它必要的组分均为翻译反应进行了优化。这些设计使得目的蛋白具有更强的稳定性和更高的表达量。
特点   . 更快地获得数据:在1小时之内可表达出蛋白,而不像体内表达那样需要几天的时间。
• 完备的体系:无需另外购买试剂。
• 得到高产率蛋白:可产生高达500µg/ml的蛋白,可进行多种下游应用。
• 反应体系可调:方便易行的筛选方案用于高通量蛋白表达。
• 灵活:可通过以下方式检测到表达出的蛋白:Coomassie®染色、掺入荧光标记的tRNA或生物素标记的tRNA。

 

L1115   S30 T7 High-Yield Protein Expression System   品牌 Promega

S30 T7 High-Yield Protein Expression System

货号: L1115      产品名称: S30 T7 High-Yield Protein Expression System   品牌: Promega 规格: 8 reactions 二周到货 生化试验

说明  
 E. coli S30 T7 High-Yield Protein Expression System(E. coli S30 T7高产率蛋白表达系统)被设计用于在1小时内表达高达500μg/ml的蛋白,本系统使用的载体为带有T7启动子和核糖体结合位点的质粒载体。本系统提供的提取物含有用于转录的T7 RNA聚合酶,并使OmpT内源蛋白酶和Ion蛋白酶活性缺失。系统内所有其它必要的组分均为翻译反应进行了优化。这些设计使得目的蛋白具有更强的稳定性和更高的表达量。
特点   . 更快地获得数据:在1小时之内可表达出蛋白,而不像体内表达那样需要几天的时间。
• 完备的体系:无需另外购买试剂。
• 得到高产率蛋白:可产生高达500µg/ml的蛋白,可进行多种下游应用。
• 反应体系可调:方便易行的筛选方案用于高通量蛋白表达。
• 灵活:可通过以下方式检测到表达出的蛋白:Coomassie®染色、掺入荧光标记的tRNA或生物素标记的tRNA。

 

20   FluoroQuest Fluorescence Quantum Yield Determination Kit *Optimized for Bioconjugates*   品牌 AAT

FluoroQuest Fluorescence Quantum Yield Determination Kit *Optimized for Bioconjugates*

货号: 20      产品名称: FluoroQuest Fluorescence Quantum Yield Determination Kit *Optimized for Bioconjugates*   品牌: AAT 规格: 1 kit 二周到货 生化试验

FluoroQuest� Fluorescence Quantum Yield Determination Kit *Optimized for Bioconjugates*
Cat # 20
Storage F/D/L (see storage codes below)
Unit Size 1 kit
When a fluorophore absorbs a photon of light, an energetically excited state is formed. The fate of this species is varied, depending upon the exact nature of the fluorophore and its surroundings, but the end result is deactivation (loss of energy) and return to the ground state. The main deactivation processes which occur are fluorescence (loss of energy by emission of a photon), internal conversion and vibrational relaxation (non-radiative loss of energy as heat to the surroundings), and intersystem crossing to the triplet manifold and subsequent non-radiative deactivation. The fluorescence quantum yield is the ratio of photons absorbed to photons emitted through fluorescence. In other words the quantum yield gives the probability of the excited state being deactivated by fluorescence rather than by another, non-radiative mechanism. The kit provides all the essential components for fluorescence quantum yield determination for biological conjugates. It is optimized for determining the fluorescence quantum yields of fluorescent protein conjugates, peptides, nucleotides and nucleic acids.
Detailed Information:   Protocol   References   MSDS
Related Products:     iFluor Rapid Tests 
Storage Codes:
  D = Desiccated
  L = Avoid light
  F = Freeze (<-15 癈)
  R = Refrigerated (2-4 癈)
  RT = Room temperature (4-50 癈)