标签归档：fluorescence

10009135-96   Caspase-3 Fluorescence Assay Kit   品牌 Cayman

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Caspase-3 Fluorescence Assay Kit

货号： 10009135-96      产品名称： Caspase-3 Fluorescence Assay Kit   品牌： Cayman 规格： 96 wells 3周

Caspase-3 Fluorescence Assay Kit
Cayman Chemical Item Number 10009135
Description Caspase-3 plays a central role in the execution of apoptosis. Activation of caspase-3 is commonly used as a biomarker for assessment of apoptosis and for understanding mechanisms of apoptosis induction. Cayman’s caspase-3 fluorescence assay kit employs a specific caspase-3 substrate, N-Ac-DEVD-N’-MC-R110, which upon cleavage by active caspase-3, generates a highly fluorescent product that can be measured using excitation and emission wavelengths of 485 and 535 nm, respectively.1↗ The kit is simple to use and can be easily adapted to high throughput screening for therapeutic compounds that may regulate activation of caspase-3. Active caspase-3 is included in the kit as a positive control or as a quantitative standard. The caspase-3 inhibitor, N-Ac-Asp-Glu-Val-Asp-CHO, is also included in the kit for verifying the specificity of the substrate.
Stability 6 months
Storage -80°C
Shipping Dry ice in continental US; may vary elsewhere

-80°C

20006   FluoroQuest Fluorescence Signal Enhancing Solution   品牌 AAT

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FluoroQuest Fluorescence Signal Enhancing Solution

货号： 20006      产品名称： FluoroQuest Fluorescence Signal Enhancing Solution   品牌： AAT 规格： 5 mL 二周到货 生化试验

FluoroQuest� Fluorescence Signal Enhancing Solution
Cat # 20006
Storage RT (see storage codes below)
Unit Size 5 mL
When proteins are conjugated to fluorescent organic dyes, fluorescence emission of the dye molecules is usually decreased, sometimes up to 50�70%. This quenching phenomenon has been acknowledged for decades, but as yet, there are no simple, practical methods to control the fluorescence of dyes conjugated to proteins, especially for dyes conjugated to immunoglobulins. We offer this FluoroQuest� fluorescence signal enhancing solution that might increase fluorescence up to 2.5-fold in cell imaging and flow analysis. This ready-to-use solution provides an effective way to increase the sensitivity of detection of fluorescent organic labels used in immunology, histochemistry, and cell biology. 
Detailed Information:   Protocol      References      MSDS
Related Products:     Fuorescence Imaging 
Storage Codes:
  D = Desiccated
  L = Avoid light
  F = Freeze (<-15 癈)
  R = Refrigerated (2-4 癈)
  RT = Room temperature (4-50 癈)

 

 

2   Fluorescein, disodium salt *Fluorescence reference standard*   品牌 AAT

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Fluorescein, disodium salt *Fluorescence reference standard*

货号： 2      产品名称： Fluorescein, disodium salt *Fluorescence reference standard*   品牌： AAT 规格： 100 mg 二周到货 生化试验

Fluorescein, disodium salt *Fluorescence reference standard*
Cat # 2
Storage F/D/L (see storage codes below)
Unit Size 100 mg
Ex (nm) 490
Em (nm) 514
MW 376.27
Solvent Water
Fluorescein, disodium salt is water-soluble version of fluorescein (see product #1). Fluorescein is a fluorophore widely used in microscopy, forensics and serology to detect latent blood stains. Fluorescein has an absorption maximum at 490 nm and emission maximum of 514 nm (in water). It has a pKa at 6.4 and multiple ionization equilibria. This leads to pH dependent absorption and emission over the range of 5 to 9. It has an isoabsorptive point at 460 nm.
Detailed Information:   Protocol   References   MSDS   Spectrum
Related Products:     iFluor Rapid Tests 
Storage Codes:
  D = Desiccated
  L = Avoid light
  F = Freeze (<-15 癈)
  R = Refrigerated (2-4 癈)
  RT = Room temperature (4-50 癈)

 

20   FluoroQuest Fluorescence Quantum Yield Determination Kit *Optimized for Bioconjugates*   品牌 AAT

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FluoroQuest Fluorescence Quantum Yield Determination Kit *Optimized for Bioconjugates*

货号： 20      产品名称： FluoroQuest Fluorescence Quantum Yield Determination Kit *Optimized for Bioconjugates*   品牌： AAT 规格： 1 kit 二周到货 生化试验

FluoroQuest� Fluorescence Quantum Yield Determination Kit *Optimized for Bioconjugates*
Cat # 20
Storage F/D/L (see storage codes below)
Unit Size 1 kit
When a fluorophore absorbs a photon of light, an energetically excited state is formed. The fate of this species is varied, depending upon the exact nature of the fluorophore and its surroundings, but the end result is deactivation (loss of energy) and return to the ground state. The main deactivation processes which occur are fluorescence (loss of energy by emission of a photon), internal conversion and vibrational relaxation (non-radiative loss of energy as heat to the surroundings), and intersystem crossing to the triplet manifold and subsequent non-radiative deactivation. The fluorescence quantum yield is the ratio of photons absorbed to photons emitted through fluorescence. In other words the quantum yield gives the probability of the excited state being deactivated by fluorescence rather than by another, non-radiative mechanism. The kit provides all the essential components for fluorescence quantum yield determination for biological conjugates. It is optimized for determining the fluorescence quantum yields of fluorescent protein conjugates, peptides, nucleotides and nucleic acids.
Detailed Information:   Protocol   References   MSDS
Related Products:     iFluor Rapid Tests 
Storage Codes:
  D = Desiccated
  L = Avoid light
  F = Freeze (<-15 癈)
  R = Refrigerated (2-4 癈)
  RT = Room temperature (4-50 癈)

40   3-Cyano-7-hydroxycoumarin *Fluorescence reference standard*   品牌 AAT

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3-Cyano-7-hydroxycoumarin *Fluorescence reference standard*

货号： 40      产品名称： 3-Cyano-7-hydroxycoumarin *Fluorescence reference standard*   品牌： AAT 规格： 25 mg 二周到货 生化试验

3-Cyano-7-hydroxycoumarin *Fluorescence reference standard*
Cat # 40
Storage F/D/L (see storage codes below)
Unit Size 25 mg
Ex (nm) 408
Em (nm) 450
MW 187.15
Solvent DMSO
3-Cyano-7-hydroxycoumarin is used as a calibration standard for 3-cyano-7-hydroxycoumarin-based enzyme substrates. It has pH-dependent fluorescence.
Detailed Information:   Protocol   References   MSDS   Spectrum
Related Products:     Vital Stains 
Storage Codes:
  D = Desiccated
  L = Avoid light
  F = Freeze (<-15 癈)
  R = Refrigerated (2-4 癈)
  RT = Room temperature (4-50 癈)