标签归档：tdt

PF060-.155ML   TDT ENZYME   品牌 Merck

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TDT ENZYME

货号： PF060-.155ML      产品名称： TDT ENZYME   品牌： Merck 规格： *** 3周到货 生化实验

TdT, Calf Thymus
Terminal Deoxynucleotidyl Transferase
Native TdT from calf thymus. Tdt is a DNA polymerase that catalyzes the addition of deoxynucleotides to the 3′ hydroxy terminus of either double of single stranded DNA in a template-independent manner.
产品信息
Form  Liquid
Formulation  775 Units in 50 mM potassium phosphate buffer, 1 mM B-mercaptoethanol, 50% glycerol, pH 7.2.
Preservative  None
Applications 
DNA Labeling
Avoid freeze/thaw  是的
Unit definition  One unit is the amount of enzyme required to transfer 1 nmol of dAMP from dATP to the 3′-OH terminus of d(A)50 in 60 min at 37°C.
商店和运输信息
Storage  -20°C
Ship  Dry Ice Only
Standard Handling

-20°C

QIA33-1EA   |TDT FRAGEL TM DNA FRAGMENT. DETECT. KIT|TUNEL法检测DNA片断化(显色法)   品牌 Merck

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|TDT FRAGEL TM DNA FRAGMENT. DETECT. KIT|TUNEL法检测DNA片断化(显色法)

货号： QIA33-1EA      产品名称： |TDT FRAGEL TM DNA FRAGMENT. DETECT. KIT|TUNEL法检测DNA片断化(显色法)   品牌： Merck 规格： *** 现货 生化实验

FragEL™ DNA Fragmentation Detection Kit, Colorimetric – TdT Enzyme
Non-isotopic kit for the detection of apoptosis at the individual cell level. Apoptotic morphology is easily detected. This kit allows the recognition of apoptotic nuclei in paraffin-embedded tissue sections, tissue cryosections, or in cell preparations fixed on slide by Fragment End Labeling (FragEL™) of DNA. Terminal deoxynucleotidyl transferase (TdT) binds to exposed ends of DNA fragments generated in response to apoptotic signals and catalyzes the template-dependent addition of biotin-labeled and unlabeled deoxynucleotides. Biotinylated nucleotides are detected using streptavidin-horseradish peroxidase (HRP) conjugate. Diaminobenzidine reacts with the labeled sample to generate an insoluble colored product at the site of DNA fragmentation. Counterstaining with methyl green aids in the morphological evaluation and characterization of normal and apoptotic cells.
产品信息
Format  Microscopy
Form  50 Tests
Detection method  Colorimetric
Species reactivity  a broad range of species
Applications 
Frozen Sections
Paraffin Sections
Assay time  4 h
Sample type  Frozen or paraffin sections or fixed-cell preparations
Kit contains  Counterstain, Proteinase K, Equilibration and Labeling Buffers, TdT Enzyme (Cat. No. PF060), Stop Buffer, Blocking Buffer, 50X Conjugate, DAB, Positive and Negative Control Slides, H2O2/Urea Tablets, Methyl Green Counterstain, and a user protocol.
商店和运输信息
Hazardous Materials Attention: Due to the nature of the Hazardous Materials in this shipment, additional shipping chargesmay be applied to your order. Certain sizes may be exempt from the additional hazardous materials shipping charges. Please contact your local sales office for more information regarding these charges.
Storage  -20°C
Ship  Dry Ice Only
Multiple Toxicity Values, refer to MSDS

-20°C