标签归档：tata

05-1531   Anti-TATA-box-binding protein, clone SL30-3-563   品牌 Millipore

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Anti-TATA-box-binding protein, clone SL30-3-563

货号： 05-1531      产品名称： Anti-TATA-box-binding protein, clone SL30-3-563   品牌： Millipore 规格： EA 3周到货 生化实验

Anti-TATA-box-binding protein, clone SL30-3-563
Species Reactivity Key Applications Host Format Antibody Type
H, M, R  WB, ChIP Mouse Purified Monoclonal Antibody
Description:
Anti-TATA-box-binding protein Antibody, clone SL30-3-563
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Specificity:
This antibody recognizes TATA-box-binding protein at the N-terminus.
Molecular Weight:
~38 kDa observed.
Epitope:
N-terminus
Immunogen:
GST-tagged recombinant protein corresponding to human TATA-box-binding protein at the N-terminus.
Clone:
SL30-3-563
Isotype:
IgG1κ
Background Information:
The TATA-box-binding protein (TBP) is a critical, eukaryotic transcription factor. TBP a part of the RNA polymerase II eukaryotic transcription complex and is required for the transcriptional initiation of RNA polymerases. TBP is considered to be a limiting factor for certain RNA promoters. Expression is ubiquitous, however the highest expression has been observed in testis and ovary. Mutations in TBP result in spinocerebellar ataxia type 17(SCA17), a cerebellar disorder classified as autosomal dominant cerebellar ataxia and characterized by atrophy to the cerebellar and cerebral regions, extrapyramidal signs, and dementia.
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Species Reactivity:
Human
Mouse
Rat
Species Reactivity Note:
Demonstrated to react with human. Predicted to react with mouse and rat based on 100% sequence homology.
Application Notes:
Chromatin Immunoprecipitation Analysis: A previous lot was used by an independent laboratory in U2OS cells. (Denisso, S., et al. (2007). The EMBO Journal. 26:944–954.)
Control:
HeLa cell lysate
Quality Assurance:
Evaluated by Western Blot in HeLa cell lysate.
estern Blot Analysis: 0.5 µg/mL of this antibody detected TATA-box-binding protein on 10 µg of HeLa cell lysate.
Purification Method:
Protein G
Presentation:
Purified mouse monoclonal IgG1κ in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Storage Conditions:
Stable for 1 year at 2-8°C from date of receipt.
UniProt Number:
P20226
Entrez Gene Number:
NP_003185
Gene Symbol:
GTF2D
TFIID
TBP
GTF2D
SCA17
TF2D
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Alternate Names:
TATA box binding protein
Transcription initiation factor TFIID TBP subunit
TATA-binding factor
TATA sequence-binding protein
TATA-box binding protein N-terminal domain
TATA-box factor
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Usage Statement:
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Key Applications:
Western Blotting
Chromatin Immunoprecipitation (ChIP)
Entrez Gene Summary:
Initiation of transcription by RNA polymerase II requires the activities of more than 70 polypeptides. The protein that coordinates these activities is transcription factor IID (TFIID), which binds to the core promoter to position the polymerase properly, serves as the scaffold for assembly of the remainder of the transcription complex, and acts as a channel for regulatory signals. TFIID is composed of the TATA-binding protein (TBP) and a group of evolutionarily conserved proteins known as TBP-associated factors or TAFs. TAFs 2°C – 8°C

17-10098   ChIPAb+ TATA Binding Protein (TBP), clone 3T3F1-3G3   品牌 Millipore

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ChIPAb+ TATA Binding Protein (TBP), clone 3T3F1-3G3

货号： 17-10098      产品名称： ChIPAb+ TATA Binding Protein (TBP), clone 3T3F1-3G3   品牌： Millipore 规格： EA 三周到货 生化实验

ChIPAb+ TATA Binding Protein (TBP) – ChIP Validated Antibody and Primer Set
Description:
ChIPAb+ TATA Binding Protein (TBP) – ChIP Validated Antibody and Primer Set
Trade Name:
Upstate (Millipore)
Product Overview:
All ChIPAb+ antibodies are individually validated for chromatin precipitation, every lot, every time. Each ChIPAb+ antibody set includes control primers (tested every lot by qPCR) to biologically validate your IP results in a locus-specific context. The qPCR protocol and primer sequences are provided, allowing researchers to validate ChIP protocols when using our antibody in their chromatin context. Each set also includes a negative control antibody to ensure specificity of the ChIP reaction.
The ChIPAb+ TATA Binding Protein (TBP) set includes the TATA Binding Protein (TBP) antibody, a negative control mouse ascites, and qPCR primers which amplify a 166 bp region of human GAPDH promoter. The TATA Binding Protein (TBP) and negative controls are supplied in a scalable "per ChIP" reaction size and can be used to functionally validate the precipitation of TATA Binding Protein (TBP)-associated chromatin.
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Specificity:
Recognizes TATA Binding Protein (TBP).
Molecular Weight:
~42 kDa
Immunogen:
Full length recombinant human TBP.
Isotype:
IgG1
Background Information:
TATA-box Binding Protein (TBP) is a general transcription factor that functions at the core of the DNA-binding multiprotein factor TFIID. Binding of TFIID to the TATA box is the initial transcriptional step of the pre-initiation complex (PIC). TBP plays a role in the activation of eukaryotic genes transcribed by RNA polymerase II and is a component of the transcription factor SL1/TIF-IB complex, which is involved in the assembly of the PIC (preinitiation complex) during RNA polymerase I-dependent transcription (Unitprot).
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Species Reactivity:
Chicken
Human
Mouse
Xenopus
Fish
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Species Reactivity Note:
Human, mouse, chicken, xenopus and zebrafish. Does not react with Drososphila.
Reactivity with other species has not been confirmed.
Non-Reactive Species:
Drosophila
Application Notes:
Chromatin Immunoprecipitation:
Sonicated chromatin prepared from HeLa cells (1 X 106 cell equivalents per IP) were subjected to chromatin immunoprecipitation using 2 µL of either Negative Ascites or 2 µL Anti-TATA Binding Protein (TBP) and the Magna ChIP™ G Kit (Cat. # 17-611).
Successful immunoprecipitation of TBP associated DNA fragments was verified by qPCR using ChIP Primers, human GAPDH promoter as a positive locus, and GAPDH coding primers as a negative locus (Please see figures).
Data is presented as percent input of each IP sample relative to input chromatin for each amplicon and ChIP sample as indicated.
Please refer to the EZ-Magna ChIP™ G (Cat. # 17-409) or EZ-ChIP™ (Cat. # 17-371) protocol for experimental details.
Western Blot Analysis:
Representative lot data.
HeLa lysates were resolved by electrophoresis, transferred to PVDF membrane and probed with Anti-TATA Binding Protein (TBP) (1:1000 dilution).
Proteins were visualized using a secondary antibody conjugated to HRP and a chemiluminescence detection system (Please see figures).
Immunohistochemistry:
A 1:1,000-1:5,000 dilution of a previous lot was used in immunohistochemistry.
Immunocytochemistry:
A 1:1,000-1:5,000 dilution of a previous lot was used in immunocytochemistry.
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Control:
Includes negative control mouse ascites and primers specific for human GAPDH promoter.
Quality Assurance:
Chromatin Immunoprecipitation:
Sonicated chromatin prepared from He -20℃