标签归档：regulated

C9470   Regulated Mammalian Expression System   品牌 Promega

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Regulated Mammalian Expression System

货号： C9470      产品名称： Regulated Mammalian Expression System   品牌： Promega 规格： 1个系统 二周到货 生化试验

说明 
 Regulated Mammalian Expression System ( 可控哺乳动物表达系统) 的特点在于本底低，可被迅速强烈地诱导，可下调哺乳动物细胞中的基因表达。可控哺乳动物表达系统基于新的开/ 关系统，开关通过嵌合的反式激活蛋白香豆素相关化合物进行迅速和灵敏的调控。纳摩尔浓度的抗生素香豆霉素即可引发嵌合反式激活蛋白同型二聚体的形成，接着与位于驱动转录目标蛋白编码序列的最小启动子上游的λ 操作子序列结合。蛋白表达水平可以通过调节香豆霉素的浓度来控制。更重要的是， 这一表达可以通过加入新生霉素而被迅速有效地关闭，新生霉素作为拮抗剂，可将反式激活蛋白二聚体解离。可控哺乳动物表达系统之所以适合基因表达的体内调控，在于引入哺乳动物细胞中的两种特别设计的质粒以及直接加入到培养基中的调控化合物－香豆霉素和新生霉素。
目的蛋白编码区被克隆进pF12A RM Flexi® 载体或pF12K RM Flexi® 载体，都是专门用于可控哺乳动物(RM) 的蛋白表达。这些载体整合有蛋白编码区上游的调控启动子序列，并且与Flexi® 载体系统兼容。以瞬时转染为例，含有目标蛋白编码区的pF12A 或pF12K RM Flexi® 载体与pReg neo 载体共转染进入哺乳动物细胞。在应答香豆霉素和新生霉素的调控过程中，pReg neo 载体被设计为表达嵌合反式激活蛋白，该蛋白可与pF12A 或pF12K RM Flexi® 载体的调控启动子区域发生作用。另外，在抗生素G-418 存在的条件下，pReg neo 载体编码的新霉素磷酸转移酶基因可允许稳定并转染细胞筛选和增殖中保持稳定。
特点   . 更好的数据：诱导表达高度可控，本底蛋白表达水平低。
• 表达水平可控：蛋白表达是剂量反应性的；蛋白表达的开启/ 关闭控制迅速而灵敏。
• 通用：与其它Flexi® 载体相兼容。

 

4415461   TQMN MOUSE LIPID REGULATED   品牌 Invitrogen

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TQMN MOUSE LIPID REGULATED

货号： 4415461      产品名称： TQMN MOUSE LIPID REGULATED   品牌： Invitrogen 规格： GENES EXP 三周到货 生化试验

TaqMan® Array Mouse Lipid Regulated Genes
The Applied Biosystems® TaqMan® Array Mouse Lipid Regulated Genes 96-well Plate contains 44 assays to Mouse Lipid Regulated associated genes and 4 assays to candidate endogenous control genes. All assays are plated in duplicate.
The panel of assays in the TaqMan® Array Mouse Lipid Regulated Genes 96-well Plate targets genes from four major ontologies: sterol metabolism, fatty acid metabolism, lipid droplet, and transcription factors. Also on the panel are three inflammation markers for validation of pro-inflammatory stimulation experiments. Gene Signature Plates are 96-well plates that are pre-configured with the most appropriate TaqMan® Gene Expression Assays for a specific biological process, pathway, or disease state. Each plate contains predefined assays and endogenous controls dried-down in the wells, ready for accurate assessment of an entire gene signature in one simple experiment.
• Affordable; minimum order of three plates; ideal for small, medium or large-sized projects
Form: Dried Down 
Format: 96-well plate 
Species: Mouse 
Label or Dye: FAM™ 
Product Size: 1 standard plate 
Purification: Standard 
Reaction Speed: Standard 
Research Category: Neurobiology,
Biomarker-Related Pathway(s),
Immunology 
Internal Probe Modification: Non-Fluorescent Quencher (3′),
MGB (Minor Groove Binder) (3′),
FAM (5′) 
Regulatory Statement: For Research Use Only. Not for use in diagnostic procedures.
Each TaqMan® Array Plate well contains a single TaqMan® Assay for one 20 µL reaction. The plates can be stored at room temperature or at 4°C.

4418846   MS LIPID REGULATED GENES, FAST   品牌 Invitrogen

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MS LIPID REGULATED GENES, FAST

货号： 4418846      产品名称： MS LIPID REGULATED GENES, FAST   品牌： Invitrogen 规格： EACH 三周到货 生化试验

TaqMan® Array, Mouse Lipid Regulated Genes, Fast 96-well
These 96-well plates are pre-configured with the most appropriate TaqMan® Gene Expression Assays for a specific biological process, pathway, or disease state. Each plate contains predefined assays and endogenous controls dried-down in the wells, ready for accurate assessment of an entire gene signature in one simple experiment. The TaqMan® Array Mouse Lipid Regulated Genes 96-well Plate contains 44 assays to Mouse Lipid Regulated associated genes and 4 assays to candidate endogenous control genes. All assays are plated in duplicate.
Form: Dried Down 
Format: 96-well plate 
Species: Mouse
Label or Dye: FAM™ 
Product Size: 1 x 96 well plate 
Reaction Speed: Fast 
Research Category: Cell Cycle & Proliferation,
Oncology,
Neurobiology,
Biomarker-Related Pathway(s),
Immunology,
Signal Transduction,
Reproduction 
Internal Probe Modification: Non-Fluorescent Quencher (3′),
MGB (Minor Groove Binder) (3′),
FAM (5′) 
Regulatory Statement: For Research Use Only. Not for use in diagnostic procedures.
Each TaqMan® Array Plate well contains a single TaqMan® Assay for one 10 µL reaction. The plates can be stored at room temperature or at 4°C.