Proteasome-Glo 3-Substrate Cell-Based Assay System
货号： G1180 产品名称： Proteasome-Glo 3-Substrate Cell-Based Assay System 品牌： Promega 规格： 10ml 二周到货 生化试验
说明
Proteasome-GloTM Cell-Based Assay(Proteasome-GloTM细胞检测试剂盒) 是一种均质的发光检测方法，能分别检测细胞培养物中蛋白酶体相关的类胰凝乳蛋白酶、类胰蛋白酶和类caspase的蛋白酶活性。26S 的蛋白酶体是一种存在于所有真核细胞中的2.5MDa 的多蛋白复合体。Proteasome-GloTM 细胞检测试剂盒在缓冲液中提供可发光的蛋白酶体底物，该试剂经过了优化，使细胞通透性、蛋白酶体活性和萤光素酶活性均达到最佳。以" 加样- 混合- 检测" 的方式加入Proteasome-GloTM 细胞学检测试剂，引发蛋白酶体剪切底物，并通过萤光素酶反应快速产生发光信号。
用于检测三种特异蛋白酶活性的发光前体底物包括：用于检测类胰凝乳蛋白酶活性的Suc-LLVY- 氨基萤光素、用于检测类胰蛋白酶活性的Z-LRR- 氨基萤光素以及用于检测类caspase 活性的Z-nLPnLD- 氨基萤光素。每种发光底物都加入到经过优化的缓冲液中，使之更适于相应的蛋白酶体活性和萤光素酶活性。将试剂加入到包含蛋白酶体的测试样品中，就能剪切底物、释放萤光素，后者经萤光素酶的作用，产生" 辉光型" 发光信号，发光强度与酶的活性或抑制性相关。
Proteasome-GloTM 3-Substrate Cell-Based Assay System(Proteasome-GloTM 3 底物细胞检测系统) 是由3 个均质的生物发光检测系统构成，能够检测细胞培养物中3 种与细胞蛋白酶体相关的蛋白水解活性( 这三种检测试剂盒均可单独购买)。
Proteasome-GloTM 3-Substrate System(Proteasome-GloTM 3底物系统) 由三种均质的发光检测试剂盒组成，用于检测纯酶。其中的任何一个试剂盒均可单独购买。
特点 . 实验结果更具有生物相关性：使用此系统可直接从细胞环境中得到蛋白酶体活性的数据。
• 方法经过简化：" 加样- 混合- 检测" 操作程序最大程度地减少了手工处理步骤，并且使该试剂盒更适于自动化操作。
• 更快速地得到结果：在加入试剂10-30 分钟后可达到最高灵敏度。
• 更高的灵敏度：发光检测模式避免了荧光方法所固有的背景信号，提供优良的信号- 背景读数。该检测试剂盒可用于384 孔板。
G1200 Proteasome-Glo 3-Substrate Cell-Based Assay System 品牌 Promega
Proteasome-Glo 3-Substrate Cell-Based Assay System
货号： G1200 产品名称： Proteasome-Glo 3-Substrate Cell-Based Assay System 品牌： Promega 规格： 50ml 二周到货 生化试验
说明
Proteasome-GloTM Cell-Based Assay(Proteasome-GloTM细胞检测试剂盒) 是一种均质的发光检测方法，能分别检测细胞培养物中蛋白酶体相关的类胰凝乳蛋白酶、类胰蛋白酶和类caspase的蛋白酶活性。26S 的蛋白酶体是一种存在于所有真核细胞中的2.5MDa 的多蛋白复合体。Proteasome-GloTM 细胞检测试剂盒在缓冲液中提供可发光的蛋白酶体底物，该试剂经过了优化，使细胞通透性、蛋白酶体活性和萤光素酶活性均达到最佳。以" 加样- 混合- 检测" 的方式加入Proteasome-GloTM 细胞学检测试剂，引发蛋白酶体剪切底物，并通过萤光素酶反应快速产生发光信号。
用于检测三种特异蛋白酶活性的发光前体底物包括：用于检测类胰凝乳蛋白酶活性的Suc-LLVY- 氨基萤光素、用于检测类胰蛋白酶活性的Z-LRR- 氨基萤光素以及用于检测类caspase 活性的Z-nLPnLD- 氨基萤光素。每种发光底物都加入到经过优化的缓冲液中，使之更适于相应的蛋白酶体活性和萤光素酶活性。将试剂加入到包含蛋白酶体的测试样品中，就能剪切底物、释放萤光素，后者经萤光素酶的作用，产生" 辉光型" 发光信号，发光强度与酶的活性或抑制性相关。
Proteasome-GloTM 3-Substrate Cell-Based Assay System(Proteasome-GloTM 3 底物细胞检测系统) 是由3 个均质的生物发光检测系统构成，能够检测细胞培养物中3 种与细胞蛋白酶体相关的蛋白水解活性( 这三种检测试剂盒均可单独购买)。
Proteasome-GloTM 3-Substrate System(Proteasome-GloTM 3底物系统) 由三种均质的发光检测试剂盒组成，用于检测纯酶。其中的任何一个试剂盒均可单独购买。
特点 . 实验结果更具有生物相关性：使用此系统可直接从细胞环境中得到蛋白酶体活性的数据。
• 方法经过简化：" 加样- 混合- 检测" 操作程序最大程度地减少了手工处理步骤，并且使该试剂盒更适于自动化操作。
• 更快速地得到结果：在加入试剂10-30 分钟后可达到最高灵敏度。
• 更高的灵敏度：发光检测模式避免了荧光方法所固有的背景信号，提供优良的信号- 背景读数。该检测试剂盒可用于384 孔板。
G8760 Proteasome-Glo Trypsin-Like Cell-Based Assay 品牌 Promega
Proteasome-Glo Trypsin-Like Cell-Based Assay
货号： G8760 产品名称： Proteasome-Glo Trypsin-Like Cell-Based Assay 品牌： Promega 规格： 10ml 二周到货 生化试验
说明
Proteasome-GloTM Cell-Based Assay(Proteasome-GloTM细胞检测试剂盒) 是一种均质的发光检测方法，能分别检测细胞培养物中蛋白酶体相关的类胰凝乳蛋白酶、类胰蛋白酶和类caspase的蛋白酶活性。26S 的蛋白酶体是一种存在于所有真核细胞中的2.5MDa 的多蛋白复合体。Proteasome-GloTM 细胞检测试剂盒在缓冲液中提供可发光的蛋白酶体底物，该试剂经过了优化，使细胞通透性、蛋白酶体活性和萤光素酶活性均达到最佳。以" 加样- 混合- 检测" 的方式加入Proteasome-GloTM 细胞学检测试剂，引发蛋白酶体剪切底物，并通过萤光素酶反应快速产生发光信号。
用于检测三种特异蛋白酶活性的发光前体底物包括：用于检测类胰凝乳蛋白酶活性的Suc-LLVY- 氨基萤光素、用于检测类胰蛋白酶活性的Z-LRR- 氨基萤光素以及用于检测类caspase 活性的Z-nLPnLD- 氨基萤光素。每种发光底物都加入到经过优化的缓冲液中，使之更适于相应的蛋白酶体活性和萤光素酶活性。将试剂加入到包含蛋白酶体的测试样品中，就能剪切底物、释放萤光素，后者经萤光素酶的作用，产生" 辉光型" 发光信号，发光强度与酶的活性或抑制性相关。
Proteasome-GloTM 3-Substrate Cell-Based Assay System(Proteasome-GloTM 3 底物细胞检测系统) 是由3 个均质的生物发光检测系统构成，能够检测细胞培养物中3 种与细胞蛋白酶体相关的蛋白水解活性( 这三种检测试剂盒均可单独购买)。
Proteasome-GloTM 3-Substrate System(Proteasome-GloTM 3底物系统) 由三种均质的发光检测试剂盒组成，用于检测纯酶。其中的任何一个试剂盒均可单独购买。
特点 . 实验结果更具有生物相关性：使用此系统可直接从细胞环境中得到蛋白酶体活性的数据。
• 方法经过简化：" 加样- 混合- 检测" 操作程序最大程度地减少了手工处理步骤，并且使该试剂盒更适于自动化操作。
• 更快速地得到结果：在加入试剂10-30 分钟后可达到最高灵敏度。
• 更高的灵敏度：发光检测模式避免了荧光方法所固有的背景信号，提供优良的信号- 背景读数。该检测试剂盒可用于384 孔板。
G8761 Proteasome-Glo Trypsin-Like Cell-Based Assay 品牌 Promega
Proteasome-Glo Trypsin-Like Cell-Based Assay
货号： G8761 产品名称： Proteasome-Glo Trypsin-Like Cell-Based Assay 品牌： Promega 规格： 5x10ml 二周到货 生化试验
说明
Proteasome-GloTM Cell-Based Assay(Proteasome-GloTM细胞检测试剂盒) 是一种均质的发光检测方法，能分别检测细胞培养物中蛋白酶体相关的类胰凝乳蛋白酶、类胰蛋白酶和类caspase的蛋白酶活性。26S 的蛋白酶体是一种存在于所有真核细胞中的2.5MDa 的多蛋白复合体。Proteasome-GloTM 细胞检测试剂盒在缓冲液中提供可发光的蛋白酶体底物，该试剂经过了优化，使细胞通透性、蛋白酶体活性和萤光素酶活性均达到最佳。以" 加样- 混合- 检测" 的方式加入Proteasome-GloTM 细胞学检测试剂，引发蛋白酶体剪切底物，并通过萤光素酶反应快速产生发光信号。
用于检测三种特异蛋白酶活性的发光前体底物包括：用于检测类胰凝乳蛋白酶活性的Suc-LLVY- 氨基萤光素、用于检测类胰蛋白酶活性的Z-LRR- 氨基萤光素以及用于检测类caspase 活性的Z-nLPnLD- 氨基萤光素。每种发光底物都加入到经过优化的缓冲液中，使之更适于相应的蛋白酶体活性和萤光素酶活性。将试剂加入到包含蛋白酶体的测试样品中，就能剪切底物、释放萤光素，后者经萤光素酶的作用，产生" 辉光型" 发光信号，发光强度与酶的活性或抑制性相关。
Proteasome-GloTM 3-Substrate Cell-Based Assay System(Proteasome-GloTM 3 底物细胞检测系统) 是由3 个均质的生物发光检测系统构成，能够检测细胞培养物中3 种与细胞蛋白酶体相关的蛋白水解活性( 这三种检测试剂盒均可单独购买)。
Proteasome-GloTM 3-Substrate System(Proteasome-GloTM 3底物系统) 由三种均质的发光检测试剂盒组成，用于检测纯酶。其中的任何一个试剂盒均可单独购买。
特点 . 实验结果更具有生物相关性：使用此系统可直接从细胞环境中得到蛋白酶体活性的数据。
• 方法经过简化：" 加样- 混合- 检测" 操作程序最大程度地减少了手工处理步骤，并且使该试剂盒更适于自动化操作。
• 更快速地得到结果：在加入试剂10-30 分钟后可达到最高灵敏度。
• 更高的灵敏度：发光检测模式避免了荧光方法所固有的背景信号，提供优良的信号- 背景读数。该检测试剂盒可用于384 孔板。
G8860 Proteasome-Glo Caspase-Like Cell-Based Assay 品牌 Promega
Proteasome-Glo Caspase-Like Cell-Based Assay
货号： G8860 产品名称： Proteasome-Glo Caspase-Like Cell-Based Assay 品牌： Promega 规格： 10ml 二周到货 生化试验
说明
Proteasome-GloTM Cell-Based Assay(Proteasome-GloTM细胞检测试剂盒) 是一种均质的发光检测方法，能分别检测细胞培养物中蛋白酶体相关的类胰凝乳蛋白酶、类胰蛋白酶和类caspase的蛋白酶活性。26S 的蛋白酶体是一种存在于所有真核细胞中的2.5MDa 的多蛋白复合体。Proteasome-GloTM 细胞检测试剂盒在缓冲液中提供可发光的蛋白酶体底物，该试剂经过了优化，使细胞通透性、蛋白酶体活性和萤光素酶活性均达到最佳。以" 加样- 混合- 检测" 的方式加入Proteasome-GloTM 细胞学检测试剂，引发蛋白酶体剪切底物，并通过萤光素酶反应快速产生发光信号。
用于检测三种特异蛋白酶活性的发光前体底物包括：用于检测类胰凝乳蛋白酶活性的Suc-LLVY- 氨基萤光素、用于检测类胰蛋白酶活性的Z-LRR- 氨基萤光素以及用于检测类caspase 活性的Z-nLPnLD- 氨基萤光素。每种发光底物都加入到经过优化的缓冲液中，使之更适于相应的蛋白酶体活性和萤光素酶活性。将试剂加入到包含蛋白酶体的测试样品中，就能剪切底物、释放萤光素，后者经萤光素酶的作用，产生" 辉光型" 发光信号，发光强度与酶的活性或抑制性相关。
Proteasome-GloTM 3-Substrate Cell-Based Assay System(Proteasome-GloTM 3 底物细胞检测系统) 是由3 个均质的生物发光检测系统构成，能够检测细胞培养物中3 种与细胞蛋白酶体相关的蛋白水解活性( 这三种检测试剂盒均可单独购买)。
Proteasome-GloTM 3-Substrate System(Proteasome-GloTM 3底物系统) 由三种均质的发光检测试剂盒组成，用于检测纯酶。其中的任何一个试剂盒均可单独购买。
特点 . 实验结果更具有生物相关性：使用此系统可直接从细胞环境中得到蛋白酶体活性的数据。
• 方法经过简化：" 加样- 混合- 检测" 操作程序最大程度地减少了手工处理步骤，并且使该试剂盒更适于自动化操作。
• 更快速地得到结果：在加入试剂10-30 分钟后可达到最高灵敏度。
• 更高的灵敏度：发光检测模式避免了荧光方法所固有的背景信号，提供优良的信号- 背景读数。该检测试剂盒可用于384 孔板。
G8861 Proteasome-Glo Caspase-Like Cell-Based Assay 品牌 Promega
Proteasome-Glo Caspase-Like Cell-Based Assay
货号： G8861 产品名称： Proteasome-Glo Caspase-Like Cell-Based Assay 品牌： Promega 规格： 5x10ml 二周到货 生化试验
说明
Proteasome-GloTM Cell-Based Assay(Proteasome-GloTM细胞检测试剂盒) 是一种均质的发光检测方法，能分别检测细胞培养物中蛋白酶体相关的类胰凝乳蛋白酶、类胰蛋白酶和类caspase的蛋白酶活性。26S 的蛋白酶体是一种存在于所有真核细胞中的2.5MDa 的多蛋白复合体。Proteasome-GloTM 细胞检测试剂盒在缓冲液中提供可发光的蛋白酶体底物，该试剂经过了优化，使细胞通透性、蛋白酶体活性和萤光素酶活性均达到最佳。以" 加样- 混合- 检测" 的方式加入Proteasome-GloTM 细胞学检测试剂，引发蛋白酶体剪切底物，并通过萤光素酶反应快速产生发光信号。
用于检测三种特异蛋白酶活性的发光前体底物包括：用于检测类胰凝乳蛋白酶活性的Suc-LLVY- 氨基萤光素、用于检测类胰蛋白酶活性的Z-LRR- 氨基萤光素以及用于检测类caspase 活性的Z-nLPnLD- 氨基萤光素。每种发光底物都加入到经过优化的缓冲液中，使之更适于相应的蛋白酶体活性和萤光素酶活性。将试剂加入到包含蛋白酶体的测试样品中，就能剪切底物、释放萤光素，后者经萤光素酶的作用，产生" 辉光型" 发光信号，发光强度与酶的活性或抑制性相关。
Proteasome-GloTM 3-Substrate Cell-Based Assay System(Proteasome-GloTM 3 底物细胞检测系统) 是由3 个均质的生物发光检测系统构成，能够检测细胞培养物中3 种与细胞蛋白酶体相关的蛋白水解活性( 这三种检测试剂盒均可单独购买)。
Proteasome-GloTM 3-Substrate System(Proteasome-GloTM 3底物系统) 由三种均质的发光检测试剂盒组成，用于检测纯酶。其中的任何一个试剂盒均可单独购买。
特点 . 实验结果更具有生物相关性：使用此系统可直接从细胞环境中得到蛋白酶体活性的数据。
• 方法经过简化：" 加样- 混合- 检测" 操作程序最大程度地减少了手工处理步骤，并且使该试剂盒更适于自动化操作。
• 更快速地得到结果：在加入试剂10-30 分钟后可达到最高灵敏度。
• 更高的灵敏度：发光检测模式避免了荧光方法所固有的背景信号，提供优良的信号- 背景读数。该检测试剂盒可用于384 孔板。
ST1049-100UL ANTI-20S PROTEASOME A1-3,5-7-SUB MO MAB 品牌 Merck
ANTI-20S PROTEASOME A1-3,5-7-SUB MO MAB
货号： ST1049-100UL 产品名称： ANTI-20S PROTEASOME A1-3,5-7-SUB MO MAB 品牌： Merck 规格： *** 3周到货 生化实验
Anti-20S Proteasome α1, 2, 3, 5, 6, & 7-Subunits Mouse mAb (MCP231)
Recognizes α1, 2, 3, 5, 6 and 7 subunits of 20S proteasome in human placental proteasome preparations.
产品信息
Host Mouse
Isotype IgG1
Immunogen dinitrophenylated proteasomes
Epitope within the prosbox I motif common to α subunits (TVWSPQGRLHQVEYAMEA)
Clone MCP231
Form Liquid
Formulation In PBS.
Species reactivity human, mouse, rabbit, rat, yeast
Preservative ≤0.1% sodium azide
Applications
Enzyme-Linked Immunosorbent Assay
Frozen Sections
Immunoblotting (Western Blotting)
Immunocytochemistry
Immunofluorescence
Immunohistochemistry
Not Immunoprecipitation
Avoid freeze/thaw 是的
Positive control Human placental tissue
商店和运输信息
Storage -20°C
Ship Blue Ice Only
Highly Toxic
-20°C
ST1053-100UL ANTI-20S PROTEASOME CORE SUBUNITS RB PAB 品牌 Merck
ANTI-20S PROTEASOME CORE SUBUNITS RB PAB
货号： ST1053-100UL 产品名称： ANTI-20S PROTEASOME CORE SUBUNITS RB PAB 品牌： Merck 规格： *** 3周到货 生化实验
Anti-20S Proteasome Core Subunits Rabbit pAb
Recognizes the ~25-30 kDa 20S proteasome core subunits (α5, α7, β1, β5i, and β7) in human erythrocytes, HeLa cells and 3T3 cells.
产品信息
Host Rabbit
Isotype IgG
Immunogen human erythrocyte proteasomes
Form Liquid
Formulation Serum diluted in PBS.
Species reactivity human, mouse, yeast
Preservative ≤0.1% sodium azide
Applications
Immunoblotting (Western Blotting)
Immunoprecipitation
Paraffin Sections
Avoid freeze/thaw 是的
Positive control HeLa cells, RSV 3T3 mouse fibroblasts, or yeast whole cell lysates
商店和运输信息
Storage -20°C
Ship Blue Ice Only
Irritant
-20°C
BML-KI340-0020 Proteasome Assay Buffer 品牌 Biomol
Proteasome Assay Buffer
货号： BML-KI340-0020 产品名称： Proteasome Assay Buffer 品牌： Biomol 规格： 20 ml 3周到货 生化实验
Assay buffer from the 20S Proteasome Assay Kit for Drug Discovery.
Product Specification
Long Term Storage:-80°C
-80°C
BML-PW0440-0025 Proteasome 19S Rpn12/S14 subunit, pAb 品牌 Biomol
Proteasome 19S Rpn12/S14 subunit, pAb
货号： BML-PW0440-0025 产品名称： Proteasome 19S Rpn12/S14 subunit, pAb 品牌： Biomol 规格： 25 ul 3周到货 生化实验
Product Specification
Host:Rabbit
Immunogen:Full-length Arabidopsis proteasome 26S (19S regulator complex) (Rpn12a/S14 subunit).
Application:Western Blot
Specificity:Recognizes Arabidopsis proteasome 26S (19S regulator complex) (Rpn12a/S14 subunit).
Formulation:Liquid. Serum.
Long Term Storage:-20°C
-20°C
BML-PW0445-0025 Proteasome 19S Rpt2/S4 subunit, pAb 品牌 Biomol
Proteasome 19S Rpt2/S4 subunit, pAb
货号： BML-PW0445-0025 产品名称： Proteasome 19S Rpt2/S4 subunit, pAb 品牌： Biomol 规格： 25 ul 3周到货 生化实验
Product Specification
Host:Rabbit
Immunogen:Full-length Arabidopsis proteasome 26S (19S regulator complex) (Rpt2a/S4 subunit).
Application:Western Blot
Specificity:Recognizes Arabidopsis proteasome 26S (19S regulator complex) (Rpt2a/S4 subunit).
Formulation:Liquid.
Long Term Storage:-20°C
-20°C
BML-PW0445-0100 Proteasome 19S Rpt2/S4 subunit, pAb 品牌 Biomol
Proteasome 19S Rpt2/S4 subunit, pAb
货号： BML-PW0445-0100 产品名称： Proteasome 19S Rpt2/S4 subunit, pAb 品牌： Biomol 规格： 100 ul 3周到货 生化实验
Product Specification
Host:Rabbit
Immunogen:Full-length Arabidopsis proteasome 26S (19S regulator complex) (Rpt2a/S4 subunit).
Application:Western Blot
Specificity:Recognizes Arabidopsis proteasome 26S (19S regulator complex) (Rpt2a/S4 subunit).
Formulation:Liquid.
Long Term Storage:-20°C
-20°C
BML-PW0450-0025 Proteasome 19S Rpn5 subunit, pAb 品牌 Biomol
Proteasome 19S Rpn5 subunit, pAb
货号： BML-PW0450-0025 产品名称： Proteasome 19S Rpn5 subunit, pAb 品牌： Biomol 规格： 25 ul 3周到货 生化实验
Product Specification
Host:Rabbit
Immunogen:Full length A. thaliana protein.
Application:Western Blot
Specificity:Recognizes A. thaliana.
Formulation:Liquid. Neat serum.
Long Term Storage:-20°C
-20°C
BML-PW0530-0025 Proteasome 19S Rpt2/S4 subunit (human), pAb 品牌 Biomol
Proteasome 19S Rpt2/S4 subunit (human), pAb
货号： BML-PW0530-0025 产品名称： Proteasome 19S Rpt2/S4 subunit (human), pAb 品牌： Biomol 规格： 25 ul 3周到货 生化实验
Product Specification
Alternative Name:26S Proteasome subunit ATPase 1
Host:Rabbit
Immunogen:Synthetic peptide corresponding to aa 368-383 of human proteasome 19S (Rpt2/S4 subunit).
UniProt ID:P62191
Application:Western Blot (1:1’000)
Optimal conditions must be determined individually for each application.
Specificity:Recognizes human proteasome 19S (Rpt2/S4 subunit).
Purity Detail:Partially purified by salt precipitation.
Formulation:Liquid. In PBS containing 0.01% sodium azide.
Use/Stability:Stable for at least 1 year after receipt when stored at -20°C. Dilute with PBS, pH 7.2-7.4, containing 1% normal goat serum (if a goat anti-rabbit IgG linker antibody is to be used). Store diluted antibody at +4°C, do not freeze and use within 1 month.
Handling:Avoid freeze/thaw cycles. After opening, prepare aliquots and store at -20°C.
Short Term Storage:+4°C
Long Term Storage:-20°C
Miscellaneous/General:The 19S regulatory complex consists of a ‘base’ sub-complex, composed of a hetero-hexameric ring of AAA-ATPases that not only function as the gate-opener to the 20S proteasome but also, in conjunction with two other high-molecular-mass non-ATPase subunits (Rpn2/S1 and Rpn1/S2), as a platform for protein unfolding. The ‘lid’ sub-complex of the 19S complex, composed of non-ATPase subunits is involved in the binding and deubiquitinylation of proteasome targeted ubiquitin conjugated proteins and assists in the transport of substrates through the ‘base’ and into the 20S catalytic core.
-20°C
BML-PW0530-0100 Proteasome 19S Rpt2/S4 subunit (human), pAb 品牌 Biomol
Proteasome 19S Rpt2/S4 subunit (human), pAb
货号： BML-PW0530-0100 产品名称： Proteasome 19S Rpt2/S4 subunit (human), pAb 品牌： Biomol 规格： 100 ul 3周到货 生化实验
Product Specification
Alternative Name:26S Proteasome subunit ATPase 1
Host:Rabbit
Immunogen:Synthetic peptide corresponding to aa 368-383 of human proteasome 19S (Rpt2/S4 subunit).
UniProt ID:P62191
Application:Western Blot (1:1’000)
Optimal conditions must be determined individually for each application.
Specificity:Recognizes human proteasome 19S (Rpt2/S4 subunit).
Purity Detail:Partially purified by salt precipitation.
Formulation:Liquid. In PBS containing 0.01% sodium azide.
Use/Stability:Stable for at least 1 year after receipt when stored at -20°C. Dilute with PBS, pH 7.2-7.4, containing 1% normal goat serum (if a goat anti-rabbit IgG linker antibody is to be used). Store diluted antibody at +4°C, do not freeze and use within 1 month.
Handling:Avoid freeze/thaw cycles. After opening, prepare aliquots and store at -20°C.
Short Term Storage:+4°C
Long Term Storage:-20°C
Miscellaneous/General:The 19S regulatory complex consists of a ‘base’ sub-complex, composed of a hetero-hexameric ring of AAA-ATPases that not only function as the gate-opener to the 20S proteasome but also, in conjunction with two other high-molecular-mass non-ATPase subunits (Rpn2/S1 and Rpn1/S2), as a platform for protein unfolding. The ‘lid’ sub-complex of the 19S complex, composed of non-ATPase subunits is involved in the binding and deubiquitinylation of proteasome targeted ubiquitin conjugated proteins and assists in the transport of substrates through the ‘base’ and into the 20S catalytic core.
-20°C
BML-PW0575-0001 Proteasome ELISA Kit 品牌 Biomol
Proteasome ELISA Kit
货号： BML-PW0575-0001 产品名称： Proteasome ELISA Kit 品牌： Biomol 规格： Kit 3周到货 生化实验
Product Specification
Assay Time:<3.5 hours
Application:For quantification of 20S proteasome levels in biological samples noting that the kit is not suitable for mouse or rat samples.
Quantity:96 wells (24 tests in triplicate)
Use/Stability:Stable for up to six months from receipt when stored at -20°C.
Handling:Avoid freeze/thaw cycles.
Long Term Storage:-20°C
Kit/Set Contains:
Plate, Capture Antibody,Detection Antibody, Conjugated Antibody, Proteasome Stock Solution, Binding Buffer, Wash Buffer, Blocking Buffer, ELISA Buffer, Lysis Buffer, TMB Substrate Solution, Stop Solution
Miscellaneous/General:Proteasomes are non-lysosomal proteolytic complexes localised primarily in the cytoplasm and in the nucleus of eukaryotic cells. The 26S proteasome structure is composed of a 20S proteasome catalytic core complex and one or two 19S regulatory subcomplexes. The 20S core comprises two copies of 14 subunits (7 α–subunits and 7 β–subunits) arranged in a α7β7β7α7 cylindrical array. Varying catalytic subunit composition (β1, β1i; β2, β2i; β5, β5i) results in a variety of possible subtypes. The 19S regulatory subcomplexes, comprised of 6 ATPase and at least 10 non-ATPase subunits, specifically bind ubiquitinylated proteins and provide the 20S core with an ATP-ubiquitin–dependent proteolytic activity. The ubiquitin-proteasome system is the major non-lysosomal system for the degradation of short half-life proteins and peptides that are involved in basic cellular processes, such as cell-cycle regulation and apoptosis, transcriptional regulation, or antigen processing. Thus, protein degradation by the ubiquitin-proteasome pathway has a major regulatory function for proliferation activity and survival of both normal and malignant cells. The 20S proteasome has been detected in normal human blood plasma (known as circulating proteasome), possessing comparatively low specific activity and with a distinct pattern of subtypes. Proteasomes are often overexpressed in cancer cells; abnormally high expression of proteasomes having been found in human leukaemia cells, renal cancer cells and in breast cancer cell lines. In patients suffering from auto-immune diseases, malignant myelo-proliferative syndromes, multiple myeloma, acute and chronic lymphatic leukaemia, solid tumour, sepsis or trauma, the concentration of circulating proteasome has been found to be elevated, to correlate with the disease state, and may have prognostic significance. Proteasome levels have been measured by enzyme-linked immunosorbent assay (ELISA) techniques in cell lysates, serum or plasma samples. This approach has been used to show that proteasome concentrations in peripheral blood are elevated in patients with certain types of malignant diseases, including multiple myeloma, suggesting that circulating proteasome levels may be correlated with tumour burden. The link between elevated circulating proteasome levels and disease activity has also been demonstrated in patients with systemic autoimmune diseases.
-20°C
BML-PW8100-0025 Proteasome 20S α6 subunit, mAb (MCP20) 品牌 Biomol
Proteasome 20S α6 subunit, mAb (MCP20)
货号： BML-PW8100-0025 产品名称： Proteasome 20S α6 subunit, mAb (MCP20) 品牌： Biomol 规格： 25 ul 3周到货 生化实验
Product Specification
Alternative Name:PROS30
Clone:MCP20
Host:Mouse
Isotype:IgG1
Immunogen:Human placenta derived proteasomes.
UniProt ID:P60900
Application:Immunohistochemistry
Immunoprecipitation: This antibody has been immobilised on CNBr-activated Sepharose CL-4B and used to precipitate proteasomes from HeLa cells. Proteasomal subunits were subsequently separated by 2D PAGE.
Weste
Specificity:Recognizes human and rabbit proteasome 20S (α6 subunit). Does not cross-react with rat or mouse protein.
Purity Detail:Partially purified ascites.
Formulation:Liquid. In PBS containing 10mM sodium azide.
Use/Stability:Aliquot undiluted antibody into smaller volumes (not less than 10µL) prior to freezing if appropriate. The use of high quality ‘antiserum-grade’ plastic or glass vials is recommended. Store diluted antibody at 2-4°C (do not freeze) and use within 1 month. Dilute to working strength with phosphate buffered saline pH 7.2-7.4 and 1% normal goat serum (if a goat anti-mouse IgG linker antibody is to be used).
Handling:Avoid freeze/thaw cycles.
Long Term Storage:-20°C
-20°C
BML-PW8100-0100 Proteasome 20S α6 subunit, mAb (MCP20) 品牌 Biomol
Proteasome 20S α6 subunit, mAb (MCP20)
货号： BML-PW8100-0100 产品名称： Proteasome 20S α6 subunit, mAb (MCP20) 品牌： Biomol 规格： 100 ul 3周到货 生化实验
Product Specification
Alternative Name:PROS30
Clone:MCP20
Host:Mouse
Isotype:IgG1
Immunogen:Human placenta derived proteasomes.
UniProt ID:P60900
Application:Immunohistochemistry
Immunoprecipitation: This antibody has been immobilised on CNBr-activated Sepharose CL-4B and used to precipitate proteasomes from HeLa cells. Proteasomal subunits were subsequently separated by 2D PAGE.
Weste
Specificity:Recognizes human and rabbit proteasome 20S (α6 subunit). Does not cross-react with rat or mouse protein.
Purity Detail:Partially purified ascites.
Formulation:Liquid. In PBS containing 10mM sodium azide.
Use/Stability:Aliquot undiluted antibody into smaller volumes (not less than 10µL) prior to freezing if appropriate. The use of high quality ‘antiserum-grade’ plastic or glass vials is recommended. Store diluted antibody at 2-4°C (do not freeze) and use within 1 month. Dilute to working strength with phosphate buffered saline pH 7.2-7.4 and 1% normal goat serum (if a goat anti-mouse IgG linker antibody is to be used).
Handling:Avoid freeze/thaw cycles.
Long Term Storage:-20°C
-20°C
BML-PW8105-0025 Proteasome 20S α2 subunit, mAb (MCP21) 品牌 Biomol
Proteasome 20S α2 subunit, mAb (MCP21)
货号： BML-PW8105-0025 产品名称： Proteasome 20S α2 subunit, mAb (MCP21) 品牌： Biomol 规格： 25 ul 3周到货 生化实验
Product Specification
Clone:MCP21
Host:Mouse
Isotype:IgG1
Immunogen:Human placenta derived proteasomes.
UniProt ID:P25787
Application:Immunohistochemistry
Immunoprecipitation
Western Blot
Optimal conditions must be determined individually for each application.
Specificity:Recognizes human, rabbit and cow proteasome 20S (α2 subunit).
Purity Detail:Partially purified ascites.
Formulation:Liquid. In PBS containing 10mM sodium azide.
Handling:Avoid freeze/thaw cycles.
Long Term Storage:-20°C
-20°C
BML-PW8105-0100 Proteasome 20S α2 subunit, mAb (MCP21) 品牌 Biomol
Proteasome 20S α2 subunit, mAb (MCP21)
货号： BML-PW8105-0100 产品名称： Proteasome 20S α2 subunit, mAb (MCP21) 品牌： Biomol 规格： 100 ul 3周到货 生化实验
Product Specification
Clone:MCP21
Host:Mouse
Isotype:IgG1
Immunogen:Human placenta derived proteasomes.
UniProt ID:P25787
Application:Immunohistochemistry
Immunoprecipitation
Western Blot
Optimal conditions must be determined individually for each application.
Specificity:Recognizes human, rabbit and cow proteasome 20S (α2 subunit).
Purity Detail:Partially purified ascites.
Formulation:Liquid. In PBS containing 10mM sodium azide.
Handling:Avoid freeze/thaw cycles.
Long Term Storage:-20°C
-20°C