标签归档：osteocalcin

ALX-210-333-C100   Osteocalcin (mouse) (NT), pAb   品牌 Alexis

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Osteocalcin (mouse) (NT), pAb

货号： ALX-210-333-C100      产品名称： Osteocalcin (mouse) (NT), pAb   品牌： Alexis 规格： 100.micro.g 三周 生化实验

Osteocalcin (mouse) (NT), pAb 
Product Specification
Host: Rabbit
Immunogen: Synthetic peptide corresponding to aa 1-20 (Y1LGASVPSPDP
LEPTREQCE20) of intracellular N-terminal mouse osteocalcin.
Application: Immunohistochemistry (paraffin sections (1-2µg/ml) after trypsin treatment)
Note: Not suitable for Western blot.
Specificity: Recognizes intracellular N-terminal mouse osteocalcin. Does not cross-react with rat osteocalcin.
Purity Detail: Protein A-affinity and epitope-affinity purified.
Formulation: Lyophilized from 10mM PBS, pH 7.4, containing 1% BSA.
Reconstitution: Reconstitute with 50µl of Reconstitution Solution (water containing 0.1% sodium azide) to a final concentration of 2mg/ml.
                Further dilutions should be made using 10mM PBS, pH 7.4, containing 1% BSA and 0.1% sodium azide.
Use/Stability: Reconstituted solution can be stored at 4°C for 6 months and at -20°C for 1 year.
Handling: Avoid freeze/thaw cycles.
Short Term Storage: +4°C
Long Term Storage: -20°C

-20°C

AB10911   Anti-Osteocalcin   品牌 Millipore

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Anti-Osteocalcin

货号： AB10911      产品名称： Anti-Osteocalcin   品牌： Millipore 规格： EA 三周到货 生化实验

Anti-Osteocalcin
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REFERENCES
In vitro and in vivo characteristics of stem cells derived from the periodontal ligament of human deciduous and permanent teeth.
Je Seon Song,Seong-Oh Kim,Seung-Hye Kim,Hyung-Jun Choi,Heung-Kyu Son,Han-Sung Jung,Chang-Sung Kim,Jae-Ho Lee (2012) Tissue engineering. Part A.18
Lineage differentiation of mesenchymal stem cells from dental pulp, apical papilla, and periodontal ligament.
Kentaro Akiyama,Chider Chen,Stan Gronthos,Songtao Shi (2012) Methods in molecular biology (Clifton, N.J.).887
Species Reactivity Key Applications Host Format Antibody Type
H, M, R  WB, IHC Rabbit Serum Polyclonal Antibody
Description:
Anti-Osteocalcin Antibody
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Specificity:
The antibody reacts with the osteocalcin protein.
Molecular Weight:
11 kDa calculated.
*Band of ~22 kDa is observed. The difference between observed and expected band may be due to post-translational modifications.
Epitope:
aa 59-74
Immunogen:
Synthetic peptide corresponding to aa 59-74 of osteocalcin protein.
Background Information:
Osteocalcin belongs to the osteocalcin/matrix Gla protein family. It constitutes 1 to 2% of the total bone protein. It is made by osteoblasts, and is a major component of the noncollagenous bone matrix. It has a high affinity for calcium and binds strongly to apatite. Sixty to ninety percent of de novo synthesized osteocalcin is incorporated into the bone matrix where it binds to hydroxyapatite during matrix mineralization. The remainder of the osteocalcin is released into the circulation where it can be measured as a sensitive marker of bone formation. Osteocalcin found in serum is almost exclusively derived from the bone formation with little or no contribution from the resorption process. Drugs acting primarily on bone resorption, such as bisphosphonates, induce a rapid decline in specific bone resorption markers, whereas osteocalcin levels only change after extended therapy periods, in accordance with the expected dynamics of a bone formation marker. Serum osteocalcin is elevated in diseases characterized by increased bone turnover such as osteoporosis, hyperparathyroidism and Paget’s disease, and low in conditions associated with low bone turnover such as hypoparathyroidism and growth hormone deficiency.
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Species Reactivity:
Human
Mouse
Rat
Species Reactivity Note:
Reacts with human, rat, and mouse.
Control:
Human heart lysate
Quality Assurance:
Routinely evaluated by Western Blot on human heart lysate.
Western Blot Analysis:
1:500 – 1:5,000 dilution of this antibody detected osteocalcin in 10 μg of human heart lysate.
Presentation:
Serum containing 0.05% sodium azide.
Storage Conditions:
Maintain at -20°C in undiluted aliquots for up to 1 year after date of receipt. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
UniProt Number:
P02818
Entrez Gene Number:
NM_199173.3
Gene Symbol:
BGP
OC
OTTHUMP00000016586
PMF1
osteocalcin
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Alternate Names:
Bone Gla protein
Gamma-carboxyglutamic acid-containing protein
bone gamma-carboxyglutamate (gla) protein (osteocalcin)
Usage Statement:
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consump -20℃

ALX-804-534-C100   Osteocalcin (gamma-carboxylated), mAb (OC4-30)   品牌 Alexis

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Osteocalcin (gamma-carboxylated), mAb (OC4-30)

货号： ALX-804-534-C100      产品名称： Osteocalcin (gamma-carboxylated), mAb (OC4-30)   品牌： Alexis 规格： 100.micro.g 3周到货 生化实验

Osteocalcin (γ-carboxylated), mAb (OC4-30)
Product Specification
Clone: OC4-30
Host: Mouse
Isotype: IgG2a
Immunogen: Bovine osteocalcin γ-carboxylated at aa 17.
Application: Immunohistochemistry (frozen and paraffin sections, 10µg/ml)
                Western Blot
                Optimal conditions must be determined individually for each application.
Specificity: Recognizes an epitope which includes the γ-carboxylated residue (aa 17) of human, rat, sheep, dog, pig, bovine, goat, rabbit and chicken γ-carboxylated (aa 17) osteocalcin. Does not cross-react with the heat decarboxylated variant of the protein.
Purity Detail: Purified by column chromatography.
Formulation: Lyophilized from 10mM PBS, pH 7.4, containing 1% BSA.
Reconstitution: Reconstitute in 50µl distilled water. Dilute in 10mM PBS, pH 7.4, containing 1% BSA and 0.1% sodium azide.
Use/Stability: After reconstitution, stable for 6 months when stored at 4°C and for 1 year when stored at -20°C.
Handling: Avoid freeze/thaw cycles.
Short Term Storage: +4°C
Long Term Storage: -20°C

-20°C

ALX-804-536-C100   Osteocalcin (21-31), mAb (OCG3)   品牌 Alexis

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Osteocalcin (21-31), mAb (OCG3)

货号： ALX-804-536-C100      产品名称： Osteocalcin (21-31), mAb (OCG3)   品牌： Alexis 规格： 100.micro.g 3周到货 生化实验

Osteocalcin (21-31), mAb (OCG3)
Product Specification
Clone: OCG3
Host: Mouse
Isotype: IgG3
Immunogen: Bovine osteocalcin
Application: Immunohistochemistry (frozen sections; weak on paraffin sections)
                Western Blot
Specificity: Recognizes aa 21-31 of human, rat, goat, sheep, pig, bovine, and rabbit osteocalcin. Does not cross-react with mouse osteocalcin.
Purity Detail: Purified by column chromatography.
Formulation: Lyophilized from 20mM TBS, pH 7.5, containing 1% BSA and 0.1% sodium azide.
Reconstitution: Reconstitute with 50µl distilled water. Dilute with 10mM TBS*, pH 7.5, containing 1% BSA and 0.1% sodium azide**.
                *) TBS should be used for dilution, as the antibody has a tendency to precipitate in PBS.
               **) When stored at +4°C, 0.1% sodium azide should be added as a preservative.
Use/Stability: Stable for at least 2 years after receipt when stored at +4°C. Reconstituted antibody is stable for 1 year when stored at -20°C and for 6 months when stored at +4°C (after adding 0.1% sodium azide). Be sure to store the antibody at a minimum concentration of 2.0mg/ml. A lower concentration may result in decreased stability. Diluted antibody should not be stored.
Handling: Avoid freeze/thaw cycles. After reconstitution, prepare aliquots and store at -20°C.
Short Term Storage: +4°C

+4°C

ALX-804-537-C100   Osteocalcin, mAb (4-9) (OCG4)   品牌 Alexis

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Osteocalcin, mAb (4-9) (OCG4)

货号： ALX-804-537-C100      产品名称： Osteocalcin, mAb (4-9) (OCG4)   品牌： Alexis 规格： 100.micro.g 3周到货 生化实验

Osteocalcin, mAb (4-9) (OCG4)
Product Specification
Clone: OCG4
Host: Mouse
Isotype: IgG1
Immunogen: Bovine osteocalcin.
Application: Immunohistochemistry (frozen sections, paraffin sections, 10µg/ml)
                Western Blot
Specificity: Recognizes aa 4-9 of human, goat, sheep, dog, pig, bovine, rabbit and chicken osteocalcin. Does not cross-react with mouse or rat osteocalcin.
Purity Detail: Affinity purified.
Formulation: Lyophilized from 10mM PBS, pH 7.4, containing 1% BSA. Does not contain any preservatives.
Reconstitution: Reconstitute with 50µl distilled water. Dilute with 10mM PBS, pH 7.4, 1% BSA and 0.1% sodium azide.
Use/Stability: Reconstituted solution is stable for 6 months when stored at +4°C (after adding 0.1% sodium azide as a preservative) and for 1 year when stored at -20°C. 
Handling: Dilute only prior to immediate use. Avoid freeze/thaw cycles. After reconstitution, prepare aliquots and store at -20°C.
Short Term Storage: +4°C
Long Term Storage: -20°C

-20°C

ALX-804-538-C100   Osteocalcin (undercarboxylated), mAb (GluOC4-5)   品牌 Alexis

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Osteocalcin (undercarboxylated), mAb (GluOC4-5)

货号： ALX-804-538-C100      产品名称： Osteocalcin (undercarboxylated), mAb (GluOC4-5)   品牌： Alexis 规格： 100.micro.g 3周到货 生化实验

Osteocalcin (undercarboxylated), mAb (GluOC4-5)
Product Specification
Clone: GluOC4-5
Host: Mouse
Isotype: IgG1
Immunogen: Synthetic peptide corresponding to aa 14-30 human osteocalcin decarboxylated glutamic acid residues 21 and 24.
UniProt ID: P02818
Source: Obtained by fusing the mouse myeloma cells line P3U1 with splenocytes of BALB/c mouse immunized with KLH-conjugated peptide from human osteocalcin. Purified from ascites by ammonium sulfate precipitation and anion exchange chromatography.
Application: ELISA: 5-10µg/ml. Colorimetric detection; use combination of clones GluOC4-5 (solid phase) and OCG3 (labelled) (Prod. No. ALX-804-536).
                Immunohistochemistry (paraffin sections): 5-10µg/ml. Fixation by AMeX modified method.
                Western Blot: 5-10µg/ml
Specificity: Recognizes human osteocalcin with decarboxylated glutamic acid residues 21 and 24. Cross-reacts with rat, goat, sheep, pig, bovine, rabbit and guinea pig osteocalcin. Does not cross-react with Gla-type osteocalcin.
Formulation: Lyophilized from 10mM PBS, pH 7.4, containing 1% BSA. Contains no preservative.
Reconstitution: Dissolve in 50µl distilled water to a final concentration of 2mg/ml.
Dilute with 10mM PBS, pH 7.4, containing 1% BSA and 0.1% sodium azide.
Use/Stability: Reconstituted solution can be stored at 4°C for 6 months and at -20°C for 1 year. 
Handling: Avoid freeze/thaw cycles.
Short Term Storage: +4°C
Long Term Storage: -20°C

-20°C