标签归档：neo

pmod2-neo   pMOD2-Neo   品牌 Invivogen

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pMOD2-Neo

货号： pmod2-neo      产品名称： pMOD2-Neo   品牌： Invivogen 规格： 20 μg 三周到货 生化试验

CpG-free neo
Synthetic Neomycin resistance gene in cloning plasmid
CpG-free neo confers resistance to Kanamycin in E. coli and G418 in mammalian cells. This DNA sequence is based on the Tn5 aph2 gene and encodes aminoglycoside 3‘-phosphotransferase, APH 3‘ II. The gene was modified by optimizing the codon usage and deleting the CpG motifs without changing the amino acid sequence of the wild type protein.
The synthetic neo gene is provided in the bacterial plasmid pMOD2 which is selectable with Ampicillin. To facilitate the excision and subcloning of the synthetic neo gene into another vector, this gene is flanked by a unique restriction site at the 5’ and 3’ end.
This gene is available in pMOD2 expression plasmid selectable with Ampicillin.
Bacteria Synthetic neo (pMOD2-Neo)
 

pmonon-gfp   pMONO-neo-GFP   品牌 Invivogen

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pMONO-neo-GFP

货号： pmonon-gfp      产品名称： pMONO-neo-GFP   品牌： Invivogen 规格： 20 μg 二周到货 生化试验

pMONO-neo
pMONO-neo cloning plasmids are specifically designed for strong and constitutive expression of a gene of interest in a wide variety of cell lines. They allow the selection of stable transfectants with a neomycin selectable marker. pMONO-neo plasmids carry either a multiple cloning site (MCS) or a green fluorescent protein (GFP) reporter gene downstream of the promoter.
– Strong and Ubiquitous Expression of the Transgene
– Convenient cloning of a gene of interest whith a Multiple Cloning Site
– Selectable with Kanamycin in E. coli and G418 in mammalian cells

pmonon-mcs   pMONO-neo-mcs   品牌 Invivogen

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pMONO-neo-mcs

货号： pmonon-mcs      产品名称： pMONO-neo-mcs   品牌： Invivogen 规格： 20 μg 二周到货 生化试验

pMONO-neo
pMONO-neo cloning plasmids are specifically designed for strong and constitutive expression of a gene of interest in a wide variety of cell lines. They allow the selection of stable transfectants with a neomycin selectable marker. pMONO-neo plasmids carry either a multiple cloning site (MCS) or a green fluorescent protein (GFP) reporter gene downstream of the promoter.
– Strong and Ubiquitous Expression of the Transgene
– Convenient cloning of a gene of interest whith a Multiple Cloning Site
– Selectable with Kanamycin in E. coli and G418 in mammalian cells

C9421   pReg neo Vector   品牌 Promega

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pReg neo Vector

货号： C9421      产品名称： pReg neo Vector   品牌： Promega 规格： 20ug 二周到货 生化试验

说明  
 Regulated Mammalian Expression System ( 可控哺乳动物表达系统) 的特点在于本底低，可被迅速强烈地诱导，可下调哺乳动物细胞中的基因表达。可控哺乳动物表达系统基于新的开/ 关系统，开关通过嵌合的反式激活蛋白香豆素相关化合物进行迅速和灵敏的调控。纳摩尔浓度的抗生素香豆霉素即可引发嵌合反式激活蛋白同型二聚体的形成，接着与位于驱动转录目标蛋白编码序列的最小启动子上游的λ 操作子序列结合。蛋白表达水平可以通过调节香豆霉素的浓度来控制。更重要的是， 这一表达可以通过加入新生霉素而被迅速有效地关闭，新生霉素作为拮抗剂，可将反式激活蛋白二聚体解离。可控哺乳动物表达系统之所以适合基因表达的体内调控，在于引入哺乳动物细胞中的两种特别设计的质粒以及直接加入到培养基中的调控化合物－香豆霉素和新生霉素。
目的蛋白编码区被克隆进pF12A RM Flexi® 载体或pF12K RM Flexi® 载体，都是专门用于可控哺乳动物(RM) 的蛋白表达。这些载体整合有蛋白编码区上游的调控启动子序列，并且与Flexi® 载体系统兼容。以瞬时转染为例，含有目标蛋白编码区的pF12A 或pF12K RM Flexi® 载体与pReg neo 载体共转染进入哺乳动物细胞。在应答香豆霉素和新生霉素的调控过程中，pReg neo 载体被设计为表达嵌合反式激活蛋白，该蛋白可与pF12A 或pF12K RM Flexi® 载体的调控启动子区域发生作用。另外，在抗生素G-418 存在的条件下，pReg neo 载体编码的新霉素磷酸转移酶基因可允许稳定并转染细胞筛选和增殖中保持稳定。
特点   . 更好的数据：诱导表达高度可控，本底蛋白表达水平低。
• 表达水平可控：蛋白表达是剂量反应性的；蛋白表达的开启/ 关闭控制迅速而灵敏。
• 通用：与其它Flexi® 载体相兼容。

 

E1320   pGL4.51[luc2/CMV/Neo] Vector   品牌 Promega

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pGL4.51[luc2/CMV/Neo] Vector

货号： E1320      产品名称： pGL4.51[luc2/CMV/Neo] Vector   品牌： Promega 规格： 20μg 二周到货 生化试验

说明  
 由启动子调控的Renilla 对照载体通常与实验用的萤火虫萤光素酶载体共转染，在Dual-Luciferase® 或Dual-Glo® 报告基因检测系统中使用。一般作为对照的Renilla 载体所表现出的活性水平是几乎不变的，而实验用的萤火虫萤光素酶载体因为不同的处理则变化很大。当实验用载体设计在Renilla 载体中时，可选择启动子调控的pGL4.13 萤火虫载体。pGL4.50 和pGL4.51 可用于标记细胞系，为建立稳定转染的细胞系提供选择Marker。pGL4.50 和pGL4.51 载体可标记细胞系，进行活体的生物发光成像的应用。     
特点  
 基于以下原因拥有更好的灵敏度和生物相关性 ：
• 报告基因表达更高 ：为了更适于哺乳动物细胞的表达，将合成的基因密码子更加优化。
• 降低了表达产物的背景和危险 ：去掉了隐藏的DNA 调控元件和转录因子结合位点。
• 瞬时应答更快 ：Rapid Response ™ 技术应用了去稳定萤光素酶基因。
其它的优势包括：
• 更灵活的检测选择 ：可随意选择合成luc2 (Photinus pyralis ) 或hRluc (Renilla reniformis ) 报告基。
• 可轻松地从瞬时转换到稳定细胞 ：有多种哺乳动物细胞选择标记可供选择。
• 可轻松在载体间转移 ：具有常用的多克隆位点和独特的SfiI 转移设计。

 

E1380   pFN26A(BIND)hRluc-neo FlexiVector   品牌 Promega

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pFN26A(BIND)hRluc-neo FlexiVector

货号： E1380      产品名称： pFN26A(BIND)hRluc-neo FlexiVector   品牌： Promega 规格： 20μg 二周到货 生化试验

说明  
 核受体检测可以通过传统的方法即利用上游带有核受体反应元件的最小启动子载体来完成。您也可以选择病毒元件像小鼠乳腺肿瘤病毒长末段重复启动子来检测雌激素或糖皮质激素的响应( 如pGL4.36)。很多情况下，利用这些方法进行的研究需要选择含有合适的内源核受体的细胞系，就是说每一种核受体的研究可能需要选择不同的细胞系。利用酵母双杂交原理的方法也适用于核受体的研究。核受体配基结合域与GAL4 DNA 结合域相融合，通过上游含有GAL4 上游激活序列的重复片段的最小启动子的萤火虫萤光素酶载体转染至细胞中。配基结合区域会与配基结合形成同源或异源的二聚体，以及与激活因子或抑制因子相互作用。这种单杂交系统可帮助您在很多细胞系中研究您所感兴趣的核受体。
特点    • 强劲：基于GAL4 的系统从内源性受体中去除了背景信号。
• 更灵敏：9X Gal4 经过优化，反应性增强，信噪比更高。
• 适应性强：海肾/ 新霉素的组合标记的引入，使得只需一个载体，既可用双萤光素酶检测试剂盒进行归一化，又可用选择性标记筛选生成的稳定细胞系。
• 稳定如一：仅需使用一个实验体系，就可以将所有的核受体进行比较或者研究其特征。
• 更快得到结果：去稳定化的luc2P 的萤光素酶报告基因经过优化，具有更高的灵敏度和更短的诱导时间。

psetn-lacz   pSELECT-neo-LacZ   品牌 Invivogen

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pSELECT-neo-LacZ

货号： psetn-lacz      产品名称： pSELECT-neo-LacZ   品牌： Invivogen 规格： 20 μg 三周到货 生化试验

pSELECT-neo
pSELECT-neo plasmids offer all the features necessary to express a gene of interest at high levels in a large number of cell types. pSELECT-neo plasmids contain two transcription units, the first drives the expression of the gene of interest and the second drives the expression of a neomycin selectable marker. pSELECT-neo contains a multiple cloning site (MCS) downstream of the composite promoter for convenient cloning of a gene of interest. pSELECT-neo is also available with the LacZ reporter gene and can be used as control vector.
– No transcriptional interference: the two expression cassettes are oriented in convergent directions
– Strong and ubiquitous expression of the transgene: EF1α/HTLV composite promoter
– Convenient cloning of a gene of interest with a Multiple Cloning Site
– Selectable with Kanamycin in E. coli and G418 in mammalian cells

psetn-mcs   pSELECT-neo-mcs   品牌 Invivogen

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pSELECT-neo-mcs

货号： psetn-mcs      产品名称： pSELECT-neo-mcs   品牌： Invivogen 规格： 20 μg 三周到货 生化试验

pSELECT-neo
pSELECT-neo plasmids offer all the features necessary to express a gene of interest at high levels in a large number of cell types. pSELECT-neo plasmids contain two transcription units, the first drives the expression of the gene of interest and the second drives the expression of a neomycin selectable marker. pSELECT-neo contains a multiple cloning site (MCS) downstream of the composite promoter for convenient cloning of a gene of interest. pSELECT-neo is also available with the LacZ reporter gene and can be used as control vector.
– No transcriptional interference: the two expression cassettes are oriented in convergent directions
– Strong and ubiquitous expression of the transgene: EF1α/HTLV composite promoter
– Convenient cloning of a gene of interest with a Multiple Cloning Site
– Selectable with Kanamycin in E. coli and G418 in mammalian cells

pvitro1-ngfplacz   pVITRO1-neo-GFP/LacZ   品牌 Invivogen

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pVITRO1-neo-GFP/LacZ

货号： pvitro1-ngfplacz      产品名称： pVITRO1-neo-GFP/LacZ   品牌： Invivogen 规格： 20 μg 三周到货 生化试验

pVITRO1-GFP/LacZ
Expression plasmids pVITRO1-GFP/LacZ are developed mainly for in vitro studies. pVITRO1-GFP/LacZ allow the ubiquitous and constitutive co-expression of two reporter genes: GFP (green fluorescent protein) and LacZ (β-galactosidase).
pVITRO1-GFP/LacZ plasmids can be stably transfected in mammalian cells and the reporter genes are expressed at high levels.
pVITRO1-GFP/LacZ plasmids carry two elongation factor 1 alpha (EF-1α) promoters, from rat and mouse origins combined to the CMV and SV40 enhancers respectively. pVITRO1-GFP/LacZ plasmids are available with different selectable markers that are active both in E. coli and mammalian cells.
Promoters: CMV enhancer / rat EF-1α & SV40 enhancer / mouse EF-1α
Selection markers active both in E. coli and mammalian cells:
– bsr (blasticidin resistance)
– hph (hygromycin resistance)
– neo (kanamycin/G418 resistance).
Reporter Genes:
– GFP (green fluorescent protein)
– LacZ (β-galactosidase)

pvitro1-nmcs   pVITRO1-neo-mcs   品牌 Invivogen

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pVITRO1-neo-mcs

货号： pvitro1-nmcs      产品名称： pVITRO1-neo-mcs   品牌： Invivogen 规格： 20 μg 三周到货 生化试验

pVITRO1-MCS
Expression plasmids pVITRO1-MCS are developed mainly for in vitro studies. pVITRO1-MCS allow the ubiquitous and constitutive co-expression of two genes of interest.
pVITRO1-MCS plasmids can be stably transfected in mammalian cells and the genes of interest are expressed at high levels.
pVITRO1-MCS plasmids carry two elongation factor 1 alpha (EF-1α) promoters, from rat and mouse origins combined to the CMV and SV40 enhancers respectively. pVITRO1-MCS plasmids are available with different selectable markers that are active both in E. coli and mammalian cells.
pVITRO1-mcs plasmids contain two multiple cloning sites (MCS) for the convenient cloning of your cDNAs of interest.
Promoters: CMV enhancer / rat EF-1α & SV40 enhancer / mouse EF-1α
Selection markers active both in E. coli and mammalian cells:
– bsr (blasticidin resistance)
– hph (hygromycin resistance)
– neo (kanamycin/G418 resistance).
Cloning sites:
– MCS1: 5’- Bsp EI, Bst 1107I, Bam HI, Bsi WI, Avr II -3’
– MCS2: 5’- Age I, Eco RV, Bgl II, Bsr GI, Nhe I -3’

pvitro2-ngfplacz   pVITRO2-neo-GFP/LacZ   品牌 Invivogen

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pVITRO2-neo-GFP/LacZ

货号： pvitro2-ngfplacz      产品名称： pVITRO2-neo-GFP/LacZ   品牌： Invivogen 规格： 20 μg 三周到货 生化试验

pVITRO2-GFP/Lacz
Expression plasmids pVITRO2-GFP/Lacz are developed mainly for in vitro studies.
pVITRO2-GFP/Lacz allows the ubiquitous and constitutive co-expression of two reporter genes: GFP (green fluorescent protein) and LacZ (β-galactosidase). pVITRO2-GFP/Lacz plasmids can be stably transfected in mammalian cells and the reporter genes are expressed at high levels.
pVITRO2-GFP/Lacz plasmids carry two human ferritin composite promoters, FerH (heavy chain) and FerL (light chain). To eliminate the iron regulation, their 5’UTRs have been replaced by the 5’UTR of the mouse and chimpanzee EF-1α genes. pVITRO2-GFP/Lacz plasmids are available with different selectable markers that are active both in E. coli and mammalian cells.
Promoters: SV40/hFerH/mEF1α & CMV/hFerL/chEF1α
Selection markers active both in E. coli and mammalian cells:
– bsr (blasticidin resistance)
– hph (hygromycin resistance)
– neo (kanamycin/G418 resistance).
Reporter Genes:
– GFP (green fluorescent protein)
– LacZ (β-galactosidase)

pvitro2-nmcs   pVITRO2-neo-mcs   品牌 Invivogen

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pVITRO2-neo-mcs

货号： pvitro2-nmcs      产品名称： pVITRO2-neo-mcs   品牌： Invivogen 规格： 20 μg 三周到货 生化试验

pVITRO2-MCS
Expression plasmids pVITRO2-MCS are developed mainly for in vitro studies.
pVITRO2-MCS allows the ubiquitous and constitutive co-expression of two genes of interest.
pVITRO2-MCS plasmids carry two human ferritin composite promoters, FerH (heavy chain) and FerL (light chain). To eliminate the iron regulation, their 5’UTRs have been replaced by the 5’UTR of the mouse and chimpanzee EF-1α genes. pVITRO2-MCS plasmids are available with different selectable markers that are active both in E. coli and mammalian cells.
pVITRO2-MCS plasmids contain two multiple cloning sites (MCS) for the convenient cloning of your cDNAs of interest.
Promoters: SV40/hFerH/mEF1α & CMV/hFerL/chEF1α
Selection markers active both in E. coli and mammalian cells:
– bsr (blasticidin resistance)
– hph (hygromycin resistance)
– neo (kanamycin/G418 resistance).
Cloning sites:
– MCS1: 5’- Age I, Eco RV, Bam HI, Mlu I, Cla I, Sal I, Avr II -3’
– MCS2: 5’- Sgr AI, Fsp I, Bgl II, Eco RI, Bst BI, Xho I, Nhe I -3’