标签归档：myeloperoxidase

4744-100   Myeloperoxidase, Human Neutrophil   品牌 Biovision

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Myeloperoxidase, Human Neutrophil

货号： 4744-100      产品名称： Myeloperoxidase, Human Neutrophil   品牌： Biovision 规格： 100 μg 3周到货 生化实验

Myeloperoxidase, Human Neutrophil
A lysosomal protein
Product Overview
Alternate Names / Synonyms: MPO, Peroxidase, myelo
Gene Symbol: MPO
Accession #: P05164
Gene ID: 4353
Source: Human Neutrophil
Appearance: Lyophilized protein
Physical Form Description: Lyophilized from 50 mM Na acetate, pH 6.0 with 100 mM NaCl
Molecular Weight: 150.0 kDa
Purity by SDS-PAGE: ≥95%
Purity by HPLC: N/A
Endotoxin Level: N/A
Biological Activity: 180-220 units per mg protein after lyophilization. One unit of activity is defined as the amount of enzyme that will decompose one micromole of hydrogen peroxide per minute at 25℃ , pH 6.1. Reaction mixture contains 33 mM sodium phosphate, pH 6.1, 30 mM guaiacol, and 0.0012% (0.35 mM) hydrogen peroxide
Reconstitution Instructions: Deionized water
Storage Temp.: -20°C
Shipping: Gel pack
Background Information: Myeloperoxidase (MPO) is a hemeprotein that belongs to the XPO subfamily of the heme peroxidase superfamily.This heme protein catalyzes oxidations by hydrogen peroxide, including MPO- chloride mediated killing of microbes and tumor cells, inactivation of hemotactic factors, crosslinking of proteins and inclination of proteins.
Handling: Centrifuge the vial prior to opening.
Usage: For Research Use Only! Not to be used in humans.

-20°C

10006438-2   Myeloperoxidase Chlorination Assay Kit   品牌 Cayman

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Myeloperoxidase Chlorination Assay Kit

货号： 10006438-2      产品名称： Myeloperoxidase Chlorination Assay Kit   品牌： Cayman 规格： 2 x 96 wells 3周

Myeloperoxidase Chlorination Assay Kit
Cayman Chemical Item Number 10006438
Description
Myeloperoxidase (MPO) is a member of the heme peroxidase superfamily and is stored within the azurophilic granules of leukocytes.1↗ MPO is found within circulating neutrophils, monocytes, and some tissue macrophages.2↗ A unique activity of MPO is its ability to use chloride as a cosubstrate with hydrogen peroxide (H2O2) to generate chlorinating oxidants such as hypochlorous acid, a potent antimicrobial agent.3↗ Recently, evidence has emerged that MPO-derived oxidants contribute to tissue damage and the initiation and propagation of acute and chronic vascular inflammatory diseases.4↗,5↗ The fact that circulating levels of MPO have been shown to predict risks for major adverse cardiac events and that levels of MPO-derived chlorinated compounds are specific biomarkers for disease progression, has attracted considerable interest in the development of therapeutically useful MPO inhibitors.6↗ MPO also oxidizes a variety of substrates, including phenols and anilines, via the classic peroxidation cycle. The relative concentrations of chloride and the reducing substrate determine whether MPO uses H2O2 for chlorination or peroxidation. Assays based on measurement of chlorination activity are more specific for MPO than those based on peroxidase substrates because peroxidases generally do not produce hypochlorous acid. The only exception is eosinophil peroxidase that produces hypochlorous acid at pH below 5. The chlorination activity of MPO has a neutral pH optimum, therefore the assay conditions can be set so that only MPO activity is specifically measured. Cayman’s Myeloperoxidase Chlorination Assay provides a convenient fluorescence-based method for detecting the MPO chlorination activity in both crude cell lysates and purified enzyme preparations. The assay utilizes the non-fluorescent 2-[6-(4-aminophenoxy)-3-oxo-3H-xanthen-9-yl]-benzoic acid (APF), which is selectively cleaved by hypochlorite (-OCl) to yield the highly fluorescent compound fluorescein.7↗ Fluorescein fluorescence is analyzed with an excitation wavelength of 480-490 nm and an emission wavelength of 515-520 nm. The kit includes a MPO-specific inhibitor for distinguishing MPO activity from MPO-independent fluorescence.
Synonyms MPO Chlorination Assay Kit
Formal Name
Stability 6 months
Storage 4°C
Shipping Wet ice in continental US; may vary elsewhere

4°C

ALX-200-600-U010   Myeloperoxidase (human leukocytes)   品牌 Alexis

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Myeloperoxidase (human leukocytes)

货号： ALX-200-600-U010      产品名称： Myeloperoxidase (human leukocytes)   品牌： Alexis 规格： 10 U 三周 生化实验

Myeloperoxidase (human leukocytes)
Product Specification
Alternative Name: MPO
MW: ~118kDa.
Source: Isolated from human leukocytes. Negative for HBsAG-, HCV- and HIV-antibodies.
EC: 1.11.1.7
CAS: 9003-99-0
UniProt ID: P05164
Formulation: Lyophilized from 0.02M sodium acetate buffer, pH 6.0.
Purity: ≥95% (SDS-PAGE). 
Specific Activity: ~250-300U/mg protein. One unit is defined as the amount of enzyme that produces an increase in absorbance at 470nm of 1.0 per min.
                         at 25°C, pH 7.0 (calculated from the initial rate of reaction using 1% guaiacol as substrate; initial H2O2 concentration 6mmol/l).
Reconstitution: Reconstitute with 1ml distilled water.
Long Term Storage: -20°C

-20°C

ALX-200-600-U025   Myeloperoxidase (human leukocytes)   品牌 Alexis

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Myeloperoxidase (human leukocytes)

货号： ALX-200-600-U025      产品名称： Myeloperoxidase (human leukocytes)   品牌： Alexis 规格： 25 U 三周 生化实验

Myeloperoxidase (human leukocytes)
Product Specification
Alternative Name: MPO
MW: ~118kDa.
Source: Isolated from human leukocytes. Negative for HBsAG-, HCV- and HIV-antibodies.
EC: 1.11.1.7
CAS: 9003-99-0
UniProt ID: P05164
Formulation: Lyophilized from 0.02M sodium acetate buffer, pH 6.0.
Purity: ≥95% (SDS-PAGE). 
Specific Activity: ~250-300U/mg protein. One unit is defined as the amount of enzyme that produces an increase in absorbance at 470nm of 1.0 per min.
                         at 25°C, pH 7.0 (calculated from the initial rate of reaction using 1% guaiacol as substrate; initial H2O2 concentration 6mmol/l).
Reconstitution: Reconstitute with 1ml distilled water.
Long Term Storage: -20°C

-20°C

ALX-200-600-5010   Myeloperoxidase (human leukocytes)   品牌 Alexis

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Myeloperoxidase (human leukocytes)

货号： ALX-200-600-5010      产品名称： Myeloperoxidase (human leukocytes)   品牌： Alexis 规格： 5×10 U 三周 生化实验

Myeloperoxidase (human leukocytes)
Product Specification
Alternative Name: MPO
MW: ~118kDa.
Source: Isolated from human leukocytes. Negative for HBsAG-, HCV- and HIV-antibodies.
EC: 1.11.1.7
CAS: 9003-99-0
UniProt ID: P05164
Formulation: Lyophilized from 0.02M sodium acetate buffer, pH 6.0.
Purity: ≥95% (SDS-PAGE). 
Specific Activity: ~250-300U/mg protein. One unit is defined as the amount of enzyme that produces an increase in absorbance at 470nm of 1.0 per min.
                         at 25°C, pH 7.0 (calculated from the initial rate of reaction using 1% guaiacol as substrate; initial H2O2 concentration 6mmol/l).
Reconstitution: Reconstitute with 1ml distilled water.
Long Term Storage: -20°C

-20°C

ALX-200-600-5025   Myeloperoxidase (human leukocytes)   品牌 Alexis

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Myeloperoxidase (human leukocytes)

货号： ALX-200-600-5025      产品名称： Myeloperoxidase (human leukocytes)   品牌： Alexis 规格： 5×25 U 三周 生化实验

Myeloperoxidase (human leukocytes)
Product Specification
Alternative Name: MPO
MW: ~118kDa.
Source: Isolated from human leukocytes. Negative for HBsAG-, HCV- and HIV-antibodies.
EC: 1.11.1.7
CAS: 9003-99-0
UniProt ID: P05164
Formulation: Lyophilized from 0.02M sodium acetate buffer, pH 6.0.
Purity: ≥95% (SDS-PAGE). 
Specific Activity: ~250-300U/mg protein. One unit is defined as the amount of enzyme that produces an increase in absorbance at 470nm of 1.0 per min.
                         at 25°C, pH 7.0 (calculated from the initial rate of reaction using 1% guaiacol as substrate; initial H2O2 concentration 6mmol/l).
Reconstitution: Reconstitute with 1ml distilled water.
Long Term Storage: -20°C

-20°C

5012-100   Anti-Human Myeloperoxidase (MPO) Antibody (Clone 03D03)   品牌 Biovision

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Anti-Human Myeloperoxidase (MPO) Antibody (Clone 03D03)

货号： 5012-100      产品名称： Anti-Human Myeloperoxidase (MPO) Antibody (Clone 03D03)   品牌： Biovision 规格： 100 μg 3周到货 生化实验

Anti-Human Myeloperoxidase (MPO) Antibody (Clone 03D03)
Anti-Human Myeloperoxidase (MPO) Monoclonal Antibody
Product Overview
Product Name: Anti-Human Myeloperoxidase (MPO) Antibody (Clone 03D03)
Antibody Target: Myeloperoxidase (MPO)
Alternative Name: 89 kDa myeloperoxidase, 84 kDa myeloperoxidase, Myeloperoxidase light chain, Myeloperoxidase heavy chain
Host: Mouse
Antibody Type: Monoclonal
Isotype: Rabbit IgG1
Immunogen: Amino acids corresponding to 138-153 of human Mouse IgG1κ
Accession#: P05164
Gene ID: 4353
Appearance: Colorless liquid
Concentration: N/A
Formulation: 0.15 M PBS
Purification: Protein A chromatography from in vitro production
Species Reactivity: Human. Does not cross-react with human lactoferrin or bovine lactoperoxidases.
Application: ELISA. Pairs as capture with Cat # 5013-100 as tracer, FACS.
Application & Usage: Myeloperoxidase Sandwich ELISA (Cat # 5012-100 as capture and Cat # 5013-100 as tracer antibody). This pair is able to detect MPO at < 3 ng/ml.
Storage Temp.: -20 °C
Shipping: Gel Pack
Background Descriptions: Myeloperoxidase (MPO) is highly expressed in neutrophils and polymorphonuclear luekocytes. MPO is expressed as a dimer in regular neutrophils with a 140 kDA. The glycosylated form of MPO has a molecular weight of 92 kDa and when undergoes a final glycosylation, MPO forms subunits of 60 kDa and 12 kDa. In recent studies, MPO was shown to be involved in the initiation and progression of cardiovascular disease.
Handling: The antibody solution should be gently mixed before use
USAGE: For Research Use Only! Not For Use in Humans.

-20°C