标签归档：mcm

ABC157   Anti-MCM7   品牌 Millipore

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Anti-MCM7

货号： ABC157      产品名称： Anti-MCM7   品牌： Millipore 规格： EA 三周到货 生化实验

Anti-MCM7
Species Reactivity Key Applications Host Format Antibody Type
H, R, Rhesus Macaque, Chp  WB Rabbit Purified Polyclonal Antibody
Description:
Anti-MCM7 Antibody
Promotional Text:
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Specificity:
This antibody recognizes the N-terminus of MCM7
Molecular Weight:
~82 kDa observed
Epitope:
N-terminus
Immunogen:
KLH-conjugated linear peptide corresponding to the N-terminus of human MCM7.
Background Information:
MCM7 is a highly conserved mini-chromosome maintenance protein (MCM) that is involved in the initiation of eukaryotic genome replication. It forms a hexameric complex with MCM4 and MCM6 (two subunits of MCM7, two subunits of MCM4 and two subunits of MCM7) and this complex regulates the helicase activity of the pre-replication complex. This complex is also involved in the formation of replication forks and in the recruitment of other DNA replication related proteins. MCM7 is reported to bind to DNA in metaphase II in oocytes and remains associated with the DNA until the S-phase. Two isoforms of MCM7 have been reported, 61 kDa and 90 kDa. In neuroblastoma tumors MCM7 expression is shown to correlates with MYCN (v-myc myelocytomatosis viral related oncogene, neuroblastoma derived) amplification where in MCM7 is rapidly up-regulated by Inducible MYCN Expression. MYCN protein is shown to bind specifically to the MCM7 promoter region, which possesses an E-box binding site for the Myc oncoproteins.
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Species Reactivity:
Human
Rat
Rhesus Macaque
Chimpanzee
Species Reactivity Note:
Demonstrated to react with Human and Rat. Predicted to react with Rhesus Macaque and Chimpanzee based on 100% sequence homology. Other homologies: Mouse (71% sequence homology).
Application Notes:
Western Blot Analysis: 0.5 µg/mL from a representative lot detected MCM7 in 10 µg of L6 cell lysate.
Control:
HeLa cell lysate
Quality Assurance:
Evaluated by Western Blot in HeLa cell lysate.
Western Blot Analysis: 0.5 µg/mL of this antibody detected MCM7 in 10 µg of HeLa cell lysate.
Purification Method:
Protein A purified
Presentation:
Purified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Storage Conditions:
Stable for 1 year at 2-8°C from date of receipt.
UniProt Number:
P33993
Entrez Gene Number:
NP_005907
Gene Symbol:
MCM7
CDC47
MCM2
Alternate Names:
DNA replication licensing factor MCM7
CDC47 homolog
P1.1-MCM3
Usage Statement:
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Key Applications:
Western Blotting
Product Name:
Anti-MCM7
Concentration:
Please refer to the Certificate of Analysis for the lot-specific concentration.
Antibody Type:
Polyclonal Antibody
Qty/Pk:
100 μg
Format:
Purified
Host:
Rabbit
Applications:
This MCM7 antibody is validated for use in WB for the detection of the MCM7 protein.

2-8℃

06-1296   Anti-MCM4   品牌 Millipore

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Anti-MCM4

货号： 06-1296      产品名称： Anti-MCM4   品牌： Millipore 规格： EA 3周到货 生化实验

Anti-MCM4
Species Reactivity Key Applications Host Format Antibody Type
H, R  WB Rabbit Unpurified Polyclonal Antibody
Description:
Anti-MCM4 Antibody
Promotional Text:
Special Shipping Offer on Antibodies
100% Performance Guaranteed
Specificity:
This antibody recognizes MCM4 at and around the N-terminus.
Molecular Weight:
~97 kDa
Epitope:
N-terminus
Immunogen:
KLH-conjugated linear peptide corresponding to residues surrounding the N-terminus of human MCM4.
Background Information:
MCM4, minichrome maintenance deficient 4 (S. cerevisae), belongs to the super family AAA ATPase. There are 6 MCM proteins (2-7). The MCM complex is essential for initiation and DNA replication but their mechanism for regulation is poorly understood. The MCM protein family has been found in all eukaryotes but not in prokaryotes except for a MCM related protein found in Archaea. Each MCM subunit has a different affinity for one another forming its own subcomplex. MCM4, MCM6, and MCM7 bind together to form a trimeric complex sometimes referred to as the MCM core.
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Species Reactivity:
Human
Rat
Species Reactivity Note:
Proven to react with human and rat.
Application Notes:
Western Blot Analysis: A 1:1,000 dilution from a previous lot detected MCM4 in A431, Hek293, HeLa, HepG2, Jurkat, and PC3 cell lysates.
Control:
HeLa cell lysate
Quality Assurance:
Evaluated by Western Blot in HeLa cell lysate.
Western Blot Analysis: A 1:1,000 dilution of this antibody detected MCM4 in 10 µg of HeLa cell lysate.
Purification Method:
Unpurified
Presentation:
Rabbit polyclonal serum with 0.05% sodium azide.
Storage Conditions:
Stable for 1 year at -20°C from date of receipt.
Handling Recommendations: Upon first thaw, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
UniProt Number:
P33991
Entrez Gene Number:
NP_005905.1
Gene Symbol:
CDC21
CDC54
MGC33310
P1-CDC21
P1-Cdc21
hCdc21
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Alternate Names:
CDC21 homolog
DNA replication licensing factor MCM4
MCM4 minichromosome maintenance deficient 4
MCM4 minichromosome maintenance deficient 4 (S. cerevisiae)
homolog of S. pombe cell devision cycle 21
minichromosome maintenance complex component 4
minichromosome maintenance deficient 4
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Usage Statement:
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Key Applications:
Western Blotting
UniProt Summary:
FUNCTION: Involved in the control of DNA replication.
SUBCELLULAR LOCATION: Nucleus By similarity.
SEQUENCE SIMILARITIES: Belongs to the MCM family.
Contains 1 MCM domain.
Product Name:
Anti-MCM4
Antibody Type:
Polyclonal Antibody
Qty/Pk:
100 µL
Format:
Unpurified
Host:
Rabbit
Applications:
Anti-MCM4 Antibody is a rabbit polyclonal antibody for detection of MCM4 also known as CDC21 homolog ,DNA replication licensing factor MCM4 ,MCM4 minichromosome maintenance deficient 4 & has been validated in WB.

-20°C