LSD1 Inhibitor
货号： 489476-10MG 产品名称： LSD1 Inhibitor 品牌： Merck 规格： *** 3周到货 生化实验
LSD1 Inhibitor
LSD Inhibitor I, BHC110 Inhibitor I, Histone Lysine Demethylase Inhibitor III, KDM1 Inhibitor I
A bisguanidine polyamine analogue that exhibits noncompetitive and specific LSD1 inhibition, with 14.1% remaining LSD1 activity at 10 µM in vitro, and is shown to significantly increase methylation at the histone 3 lysine 4 chromatin mark (H3K4me1 and H3K4me2) dose-dependently from 0.25 µM to 10 µM, without affecting global H3K9me2 (which is not a LSD1 substrate) levels in HCT116 human colon carcinoma cells. This compound induces reexpression of several epigenetically silenced genes including SFRP1, SFRP4, SFRP5, and GATA5 more effectively when compared with siRNA treatment, particularly in the case of SFRP4 and SFRP5.
产品信息
Form Pale yellow to yellow solid
Molar mass 474.34
Protect from Light 是的
Packaged under inert gas 是的
Purity ≥95% by HPLC
Solubility H2O
Chemical formula C15H40Cl4N8
CAS number 927019-63-4
商店和运输信息
Storage -20°C
Ship Shipped with Blue Ice or with Dry Ice
Standard Handling
-20°C
489477-5MG LSD1 Inhibitor II, S2101 品牌 Merck
LSD1 Inhibitor II, S2101
货号： 489477-5MG 产品名称： LSD1 Inhibitor II, S2101 品牌： Merck 规格： *** 3周到货 生化实验
LSD1 Inhibitor II, S2101
S2101, LSD Inhibitor II, BHC110 Inhibitor II, Histone Lysine Demethylase Inhibitor IV, KDM1 Inhibitor II, MOA Inhibitor II
A cell-permeable, 2-PCPA derivative that elicits mechanism-based inhibition against LSD1 (IC50 = 0.99 µM, KI = 0.61 µM, and kinact/KI = 4560 M-1S-1), and displays much lower inhibition activity toward MAO-B (KI = 17 µM, kinact/KI = 18 M-1S-1) and MAO-A (KI = 110 µM, kinact/KI = 60 M-1S-1), with higher potency and selectivity compared with those of 2-PCPA (Cat. No. 616431) (IC50 = 184 µM, KI = 100 µM, kinact/KI = 58 M-1S-1 for LSD1, and K I = 26 µM, kinact/KI = 271 M-1S-1 for MAO-B, and K I = 5 µM,
kinact/KI = 1050 M-1S-1 for MAO-A). The inhibition of LSD1 is further confirmed in a cell-based assay, the treatment of HEK293T human cells with S2101 results in a dose-dependent increase in the level of H3K4me2, and about 50-fold stronger inhibition compared with that of 2-PCPA.
产品信息
Form White solid
Molar mass 311.75
Purity ≥95% by HPLC
Solubility DMSO or H2O
Chemical formula C16H16ClF2NO
商店和运输信息
Storage +2°C to +8°C
Ship Ambient Temperature Only
Standard Handling
2°C – 8°C
489478-10MG LSD1 Inhibitor III, CBB1007 品牌 Merck
LSD1 Inhibitor III, CBB1007
货号： 489478-10MG 产品名称： LSD1 Inhibitor III, CBB1007 品牌： Merck 规格： *** 3周到货 生化实验
LSD1 Inhibitor III, CBB1007
Methyl-3-(4-(4-carbamimidoylbenzoyl)piperazine-1-carbonyl)-5-((4-carbamimidoylpiperazin-1-yl)methyl)benzoate, 3TFA, KDM1 Inhibitor III, Histone Lysine Demethylase Inhibitor V, BHC110 Inhibitor III, LSD Inhibitor III
A cell-permeable amidino-guanidinium compound that acts as a potent, reversible and substrate competitive LSD1 selective inhibitor (IC50 = 5.27 µM for hLSD1) and efficiently blocks LSD1-mediated demethylation of H3K4Me2 and H3K4Me (IC50 ≤ 5 µM) with no effect on H3K4Me3 and H3K9Me2, and LSD2 and JARID1A activities. Increases H3K4Me2 and H3K4Me contents (IC50 ≤ 5 µM), and causes activation of epigenetically suppressed CHRM4/M4-ArchR and SCN3A genes in F9 cells (IC50 ≤ 3.74 µM). Shown to preferentially arrest the growth of pluripotent tumors with minimal effect on non-pluripotent cancer or normal somatic cells (IC50 > 100 µM).
产品信息
Form White solid
Formulation Supplied as a trifluoroacetate salt.
Protect from Light 是的
Hygrocopic 是的
Packaged under inert gas 是的
Purity ≥99% by HPLC
Solubility DMSO
Chemical formula C27H34N8O4 • 3CF3CO2H
商店和运输信息
Storage -20°C
Ship Shipped with Blue Ice or with Dry Ice
Standard Handling
-20°C
489479-10MG LSD1 Inhibitor IV, RN-1,HCl 品牌 Merck
LSD1 Inhibitor IV, RN-1,HCl
货号： 489479-10MG 产品名称： LSD1 Inhibitor IV, RN-1,HCl 品牌： Merck 规格： *** 3周到货 生化实验
LSD1 Inhibitor IV, RN-1,HCl
Histone Lysine Demethylase Inhibitor VI, MOA Inhibitor III, 2-(1R,2S)-2-(4-(Benzyloxy)phenyl)cyclopropylamino)-1-(4-methylpiperazin-1-yl)ethanone, HCl, BHC110 Inhibitor IV, KDM1 Inhibitor IV, LSD Inhibitor IV
A cell-permeable tranylcypromine (parnate; Cat. No. 616431) analog that acts as a potent, irreversible inhibitor of lysine specific demethylase 1 (LSD1; IC50 = 70 nM in a HRP-coupled assay using H3K4Me2 peptide substrate). Forms a covalent adduct with flavin adenine dinucleotide (FAD). Shown to cross the blood brain barrier. Exhibits moderate selectivity over amine oxidases MAO-A and MAO-B (IC50 = 0.51 and 2.785 µM, respectively). Displays desirable pharmacokinetic properties (brain/plasma exposure ratio of 88.9), and impairs long-term memory without affecting short-term memory in mice (10 mg/kg, i.p.).
产品信息
Form Yellow solid
Molar mass 416
Protect from Light 是的
Hygrocopic 是的
Packaged under inert gas 是的
Purity ≥97% by HPLC
Solubility H2O or DMSO
Chemical formula C23H29N3O2 • HCl
商店和运输信息
Storage -20°C
Ship Shipped with Blue Ice or with Dry Ice
Standard Handling
-20°C
03-229 LSD1 human recombinant 品牌 Millipore
LSD1 human recombinant
货号： 03-229 产品名称： LSD1 human recombinant 品牌： Millipore 规格： EA 3周到货 生化实验
LSD1 human recombinant
Description:
LSD1 human recombinant
Trade Name:
Upstate (Millipore)
Qty/Pk:
50 µg
Product Overview:
Human recombinant LSD1 expressed in E. Coli
Background Information:
LSD1 catalyzes the oxidative demethylation of mono- and dimethylated H3K4, producing hydrogen peroxide and formaldehyde in the process. H3K4 methylation is considered a transcription-activating chromatin mark, and in vivo LSD1 is frequently found in association with the transcriptional corepressor protein CoREST and HDACs 1 or 2. LSD1 is inhibited by a number of established monoamine oxidase inhibitor drugs. That and the fact that its expression is elevated in a number of cancers may make it a promising target for drug development.
View All »
Applications:
Human recombinant LSD1 expressed in E. Coli.
Key Applications:
Enzyme Assay
Application Notes:
Demethylation enzyme assays
Usage Statement:
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
View All »
Species:
Human
Molecular Weight:
~78 kDa
Brand Family:
Upstate
Presentation:
6 mM Na2HPO4, 1.1 mM K2HPO4, pH 7.2, 82 mM sodium chloride, 1.6 mM KCl and 40% glycerol.
Storage Conditions:
Stable for 12 months at -80ºC from date of receipt. The enzyme is stable on ice for 30 to 60 min, but may lose activity with prolonged storage on ice. It is recommended that thawing and dilution of the enzyme be done within as short a time as possible before start of the assay. The remaining, unused, undiluted enzyme should be refrozen quickly by, for example, snap freezing in a dry/ice ethanol bath or liquid nitrogen. Freezing and storage of diluted enzyme is not recommended.
View All »
Product Name:
LSD1 human recombinant
Physical Form:
Frozen solution
Format:
Purified
UniProt Number:
O60341
Specific Activity:
>20U/μg. One U=1 pmol/min at 30ºC as determined by a Peroxidase-Coupled Assay with a Histone H3 dimethyl lysine 4 peptide.
Purity:
Purity >90% by SDS-PAGE
-80°C
BML-SE544-0050 LSD1 (human) (recombinant) 品牌 Biomol
LSD1 (human) (recombinant)
货号： BML-SE544-0050 产品名称： LSD1 (human) (recombinant) 品牌： Biomol 规格： 50 ug 3周到货 生化实验
LSD1 (human), (recombinant)
Highly active
Product Specification
Alternative Name:KDM1, Lysine-specific histone demethylase 1, BHC110, AOF2, KIAA0601
Sequence:N-terminal truncation of LSD1 from human cDNA. Sequence is identical to Genbank accession NM015013 (aa 151-852).
MW:78 kDa
Source:Produced in E. coli.
UniProt ID:O60341
Formulation:Liquid. In 8.1mM Na2HPO4, 1.5mM KH2PO4, pH 7.2, 138mM sodium chloride, 2.7mM KCl and 40% v/v glycerol.
Purity:≥90% (SDS-PAGE)
Purity Detail:Purified by multi-step chromatography.
Activity:Highly active in a peroxidase-coupled assay with the Histone H3 Dimethyl Lysine-4 Peptide (Prod. No. BML-P256).
Specific Activity:≥20 U/µg. One U=1 pmol/min at 30°C, 100 µM Histone H3 Dimethyl Lysine-4 Peptide (Prod. No. BML-P256).
Application:Study LSD1 kinetics and inhibitor sensitivity and also the effect of the enzyme in transcriptional regulation, cell cycle progression, and oncogenesis.
Long Term Storage:-80°C
Use/Stability:The enzyme is stable on ice for the time typically required to set up an experiment (30-60 min.), but may lose activity with prolonged storage on ice. It is recommended that thawing and dilution of the enzyme be done within as short a time as possible before start of the assay. The remaining, unused, undiluted enzyme should be refrozen quickly by, for example, snap freezing in a dry/ice ethanol bath or liquid nitrogen. Freezing and storage of diluted enzyme is not recommended.
-80°C
ABE365 Anti-LSD1 品牌 Millipore
Anti-LSD1
货号： ABE365 产品名称： Anti-LSD1 品牌： Millipore 规格： EA 三周到货 生化实验
Anti-LSD1
Species Reactivity Key Applications Host Format Antibody Type
H, M, R WB, IP Rabbit Affinity Purified Polyclonal Antibody
Description:
Anti-LSD1 Antibody
Promotional Text:
Special Shipping Offer on Antibodies
100% Performance Guaranteed
Specificity:
This antibody recognizes LSD1.
Molecular Weight:
~110 kDa observed. Uniprot describes two isoforms at ~92 kDa and ~95 kDa due to alternative splicing.
Immunogen:
Histidine-tagged recombinant protein corresponding to full length LSD1.
Background Information:
LSD1 (lysine specific demethylase 1), also known as BHC110, is a histone demethylase that specifically demethylates Lys4 of histone H3, a specific tag for epigenetic transcriptional activation, thereby acting as a corepressor. LSD1 acts by oxidizing the substrate by FAD to generate the corresponding imine that is subsequently hydrolyzed. LSD1 demethylates both mono- and tri-methylted Lys4 of histone H3, and may play a role in the repression of neuronal genes. Alone, it is unable to demethylate H3 Lys4 on nucleosomes and requires the presence of RCOR1/CoREST to achieve such activity. LSD1 may also demethylate Lys9 of histone H3, a specific tag for epigenetic transcriptional repression, thereby leading to derepression of androgen receptor target genes.
View All »
Species Reactivity:
Human
Mouse
Rat
Species Reactivity Note:
Demonstrated to react with Human, Mouse, and Rat.
Application Notes:
Immunoprecipitation Analysis: 10 µg from a representative lot immunoprecipitated LSD1 in 0.5 mg of HeLa nuclear extract.
Control:
HeLa nuclear extract
Quality Assurance:
Evaluated by Western Blot in HeLa nuclear extract.
Western Blot Analysis: 0.05 µg/mL of this antibody detected LSD1 in 10 µg of HeLa nuclear extract.
Purification Method:
Affinity purified
Presentation:
Purified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Storage Conditions:
Stable for 1 year at 2-8°C from date of receipt.
UniProt Number:
O60341
Entrez Gene Number:
NP_055828
Gene Symbol:
KDM1A
AOF2
KDM1
LSD1
Alternate Names:
Lysine-specific histone demethylase 1A
BRAF35-HDAC complex protein BHC110
Flavin-containing amine oxidase domain-containing protein 2
Usage Statement:
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
View All »
Key Applications:
Western Blotting
Immunoprecipitation
Entrez Gene Summary:
This gene encodes a nuclear protein containing a SWIRM domain, a FAD-binding motif, and an amine oxidase domain. This protein is a component of several histone deacetylase complexes, though it silences genes by functioning as a histone demethylase. Alternative splicing results in multiple transcript variants.
UniProt Summary:
FUNCTION: Histone demethylase that demethylates both ‘Lys-4’ (H3K4me) and ‘Lys-9’ (H3K9me) of histone H3, thereby acting as a coactivator or a corepressor, depending on the context. Acts by oxidizing the substrate by FAD to generate the corresponding imine that is subsequently hydrolyzed. Acts as a corepressor by mediating demethylation of H3K4me, a specific tag for epigenetic transcriptional activation. Demethylates both mono- (H3K4me1) and di-methylated (H3K4me2) H3K4me. May p 2-8℃
13555-1 LSD1 Polyclonal Antibody (aa 800-850) 品牌 Cayman
LSD1 Polyclonal Antibody (aa 800-850)
货号： 13555-1 产品名称： LSD1 Polyclonal Antibody (aa 800-850) 品牌： Cayman 规格： 1 ea
13486-1 LSD1 Polyclonal Antibody (aa 450-500) 品牌 Cayman
LSD1 Polyclonal Antibody (aa 450-500)
货号： 13486-1 产品名称： LSD1 Polyclonal Antibody (aa 450-500) 品牌： Cayman 规格： 1 ea
13553-1 LSD1 Polyclonal Antibody (aa 400-450) 品牌 Cayman
LSD1 Polyclonal Antibody (aa 400-450)
货号： 13553-1 产品名称： LSD1 Polyclonal Antibody (aa 400-450) 品牌： Cayman 规格： 1 ea
13554-1 LSD1 Polyclonal Antibody (aa 100-150) 品牌 Cayman
LSD1 Polyclonal Antibody (aa 100-150)
货号： 13554-1 产品名称： LSD1 Polyclonal Antibody (aa 100-150) 品牌： Cayman 规格： 1 ea
700126-500 LSD1 Assay DMSO 品牌 Cayman
LSD1 Assay DMSO
货号： 700126-500 产品名称： LSD1 Assay DMSO 品牌： Cayman 规格： 500 碌l
10245-100 LSD1 (human recombinant) 品牌 Cayman
LSD1 (human recombinant)
货号： 10245-100 产品名称： LSD1 (human recombinant) 品牌： Cayman 规格： 100 units 3周
10245-25 LSD1 (human recombinant) 品牌 Cayman
LSD1 (human recombinant)
货号： 10245-25 产品名称： LSD1 (human recombinant) 品牌： Cayman 规格： 25 units 3周
10245-50 LSD1 (human recombinant) 品牌 Cayman
LSD1 (human recombinant)
货号： 10245-50 产品名称： LSD1 (human recombinant) 品牌： Cayman 规格： 50 units 3周
700402-100 LSD Positive Control 品牌 Cayman
LSD Positive Control
货号： 700402-100 产品名称： LSD Positive Control 品牌： Cayman 规格： 100 碌L
700403-1.2 LSD Peptide Substrate 品牌 Cayman
LSD Peptide Substrate
货号： 700403-1.2 产品名称： LSD Peptide Substrate 品牌： Cayman 规格： 1.2 mL
700407-100 LSD Formaldehyde Standard 品牌 Cayman
LSD Formaldehyde Standard
货号： 700407-100 产品名称： LSD Formaldehyde Standard 品牌： Cayman 规格： 100 碌L
700405-150 LSD Formaldehyde Detector 品牌 Cayman
LSD Formaldehyde Detector
货号： 700405-150 产品名称： LSD Formaldehyde Detector 品牌： Cayman 规格： 150 mg
700406-2 LSD DMSO 品牌 Cayman
LSD DMSO
货号： 700406-2 产品名称： LSD DMSO 品牌： Cayman 规格： 2 ml