标签： lef

E4611   pGL4.49[luc2P/TCF-LEF RE/Hygro] Vector   品牌 Promega

pGL4.49[luc2P/TCF-LEF RE/Hygro] Vector

货号： E4611      产品名称： pGL4.49[luc2P/TCF-LEF RE/Hygro] Vector   品牌： Promega 规格： 20μg 二周到货 生化试验

说明  
 构建一个可以检测某一功能信号通路的细胞系，有助于解译参与这一信号通路的信号分子。这些工具载体是通过在载体的最小启动子（ minP ）的上游插入多个反应元件重复序列而获得的。 Promega 通过将优化的 luc2 萤火虫萤光素酶基因克隆到 pGL4 载体骨架上，设计了一系列能够报告信号通路活性的载体。这些载体同时含有潮霉素（ hygromycin ）抗性筛选标记，可用于瞬时转染实验和稳定细胞系的筛选。
提供的载体和检测的信号通路。
我们提供含有三种经过改造的不同萤火虫萤光素酶基因 ( luc2 、 luc2P 或 luc2CP ) 的最小启动子载体。 luc2 基因经过改造，去除了大部分隐藏的转录因子结合位点，并通过编码优化提高了在哺乳动物细胞中的表达效率。 luc2P 、 luc2CP 和 RapidResponse ™ 基因是在 luc2 基因的基础上增加了降解序列，从而可以调控 luc2 基因在细胞中的半衰期。 RapidResponse ™ 基因对刺激的响应更快，但是以牺牲信号强度为代价。 luc2P 基因 ( 半衰期为 1 个小时 ) 反应比 luc2 基因 ( 半衰期为 3 个小时 ) 快，信号强度也适合，而 luc2CP 基因 ( 半衰期为 0.4 个小时 ) 反应更快但信号强度最弱。最小启动子载体有含潮霉素（ hygromycin ）选择标签和不含选择标签的版本可供订购。为了提高实验效率，一些预设计的反应元件载体已经整合到了 pGL4.27 载体中。其中一些载体也可订购其稳定表达的细胞系 (GloResponse™ Cell Lines) 。
特点   •  预先设计的载体，省去克隆和验证步骤。
•  增强的报告基因表达水平： 为了更适于哺乳动物细胞的表达，将合成的基因密码子进行了优化。
•  降低了背景和表达出错的风险： 去掉了隐藏的 DNA 调控元件和转录因子结合位点。
•  瞬时应答更快： Rapid Response ™ 技术应用了去稳定萤光素酶基因。
•  可轻松地从瞬时表达转换到稳定细胞： 有多种哺乳动物细胞选择标记可供选择。

 

04-1130   Anti-LEF-1/TCF-1 a, clone EP2030Y   品牌 Millipore

Anti-LEF-1/TCF-1 a, clone EP2030Y

货号： 04-1130      产品名称： Anti-LEF-1/TCF-1 a, clone EP2030Y   品牌： Millipore 规格： EA 3周到货 生化实验

Anti-LEF-1/TCF-1 α, clone EP2030Y, Rabbit Monoclonal Antibody (04-1130)
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Species Reactivity Key Applications Host Format Antibody Type
H  WB, ICC, IHC, FC Rabbit Unpurified Monoclonal Antibody
Description:
Anti-LEF-1/TCF-1 α Antibody, clone EP2030Y, rabbit monoclonal
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Specificity:
This antibody recognizes LEF-1/TCF-1 α. Recognition mpas to an epitope in amino acids 236-242 of LEF-1. This region is found within the transactivation domain of LEF-1. However, it is not present in some splice variants.
Molecular Weight:
~55 kDa
Immunogen:
Synthetic peptide corresponding to human LEF-1/TCF-1 α.
Clone:
EP2030Y
Isotype:
IgG
Background Information:
Lymphoid Enhancer Factor-1 (LEF-1) is an HMG 1/2-like DNA binding/bending protein and is a member of the LEF/TCF transcription factor family. There are four LEF/TCF family members in mammalian systems (LEF-1, TCF-1, TCF-3 and TCF-4), and orthologs to these factors have been identified in many different species. LEF/TCFs are downstream mediators of Wnt/Wingless signals. Wnt signaling drives cell polarity, cell fate, and cell growth decisions in embryonic tissues and in post-natal tissues that continue to develop from mitotically active stem cell precursors. Misregulation of Wnt signaling is also implicated as a root cause of many different cancers, such as colon cancer, melanoma, breast cancer, prostate cancer and others. This antibody recognizes an epitope in amino acids 236-242 of LEF-1. This region is found within the transactivation domain of LEF-1. However, it is not present in some splice variants.
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Species Reactivity:
Human
Species Reactivity Note:
Human
Application Notes:
Immunohistochemistry Analysis: A 1:50 dilution from a representative lot detected LEF-1/TCF-1 α in human colon adenocarcinoma tissue.
Immunocytochemistry Analysis: A 1:100-250 dilution from a representative lot was used in IC.
Flow Cytometry: A 1:20 dilution of a representative lot was used in flow cytometry.
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Control:
Jurkat cell lysate
Quality Assurance:
Evaluated by Western Blot on Jurkat cell lysates.
Western Blot Analysis: A 1:25,000-50,000 dilution of this antibody was used to detect LEF-1/TCF-1 α in Jurkat cell lysate.
Purification Method:
Unpurified
Presentation:
Rabbit Monoclonal in buffer containing 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl containing 40% Glycerol, 0.01% sodium azide and 0.05% BSA.
Storage Conditions:
Stable for 1 year at -20ºC from date of receipt.
Handling Recommendations: Upon first thaw, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance. Note: Variability in freezer temperatures below -20°C may cause glycerol containing solutions to become frozen during storage.
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UniProt Number:
Q9UJU2
Entrez Gene Number:
NM_016269.2
Gene Symbol:
LEF-1
TCF1ALPHA
TCF1-alpha
Alternate Names:
lymphoid enhancer-binding factor 1
T cell-specific transcription factor 1-alpha
Usage Statement:
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro d -20°C

04-1159   Anti-LEF-1/TCF-1 a, clone EPR2029Y, Rabbit Monoclonal   品牌 Millipore

Anti-LEF-1/TCF-1 a, clone EPR2029Y, Rabbit Monoclonal

货号： 04-1159      产品名称： Anti-LEF-1/TCF-1 a, clone EPR2029Y, Rabbit Monoclonal   品牌： Millipore 规格： EA 3周到货 生化实验

Anti-LEF-1/TCF-1 α, clone EPR2029Y, Rabbit Monoclonal Antibody (04-1159)
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Species Reactivity Key Applications Host Format Antibody Type
H  WB, ICC, IH(P) Rabbit Unpurified Monoclonal Antibody
Description:
Anti-LEF-1/TCF-1 α Antibody, clone EPR2029Y, rabbit monoclonal
Promotional Text:
Special Shipping Offer on Antibodies
100% Performance Guaranteed
Specificity:
This antibody recognizes an epitope in amino acids 236-242 of LEF-1. This region is found within the transactivation domain of LEF-1. However, it is not present in some splice variants.
Molecular Weight:
~25/57 kDa
Epitope:
a.a. 236-242
Immunogen:
Synthetic peptide corresponding to residues in human LEF-1/TCF-1 α.
Clone:
EPR2029Y
Isotype:
IgG
Background Information:
Lymphoid Enhancer Factor-1 (LEF-1) is an HMG 1/2-like DNA binding/bending protein and is a member of the LEF/TCF transcription factor family. There are four LEF/TCF family members in mammalian systems (LEF-1, TCF-1, TCF-3 and TCF-4), and orthologs to these factors have been identified in many different species. LEF/TCFs are downstream mediators of Wnt/Wingless signals. Wnt signaling drives cell polarity, cell fate and cell growth decisions in embryonic tissues and in post-natal tissues that continue to develop from mitotically active stem cell precursors. Misregulation of Wnt signaling is also implicated as a root cause of many different cancers, such as colon cancer, melanoma, breast cancer, prostate cancer and others.
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Species Reactivity:
Human
Species Reactivity Note:
Human
Application Notes:
Immunohistochemistry (Paraffin) Analysis: A 1:100-250 dilution from a previous lot detected LEF-1/TCF-1 α in human tonsil tissue.
Immunocytochemistry Analysis: A 1:100-250 dilution from a previous lot was used in IC.
Control:
Jurkat cell lysate
Quality Assurance:
Evaluated by Western Blot on Jurkat cell lysates.
Western Blot Analysis: A 1:10,000-20,000 dilution of this antibody was used to detect LEF-1/TCF-1 α in Jurkat cell lysate.
Purification Method:
Unpurified
Presentation:
Rabbit Monoclonal in buffer containing 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl containing 40% Glycerol, 0.01% sodium azide and 0.05% BSA.
Storage Conditions:
Stable for 1 year at -20ºC from date of receipt.
Handling Recommendations: Upon first thaw, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance. Note: Variability in freezer temperatures below -20°C may cause glycerol containing solutions to become frozen during storage.
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UniProt Number:
Q9UJU2
Entrez Gene Number:
NM_016269.2
Gene Symbol:
DKFZp586H0919
LEF-1
TCF1-alpha
TCF1ALPHA
Alternate Names:
T cell-specific transcription factor 1-alpha
lymphoid enhancer binding factor-1
lymphoid enhancer-binding factor 1
Usage Statement:
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Key Applications:
Western Blotting
Immunocytochemistry
Immunohistochem -20°C

05-602   ANTI-LEF, CLONE 2D12   品牌 Millipore

ANTI-LEF, CLONE 2D12

货号： 05-602      产品名称： ANTI-LEF, CLONE 2D12   品牌： Millipore 规格： EA 3周到货 生化实验

Anti-LEF Antibody, clone 2D12
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REFERENCES
Regulation of LEF-1/TCF transcription factors by Wnt and other signals.
Eastman, Q and Grosschedl, R (1999) Curr. Opin. Cell Biol., 11: 233-40 (1999)
Species Reactivity Key Applications Host Format Antibody Type
H, M  WB Mouse Purified Monoclonal Antibody
Description:
Anti-LEF Antibody, clone 2D12
Promotional Text:
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Trade Name:
Upstate (Millipore)
Specificity:
LEF that appears as multiple polypeptides
Molecular Weight:
55-57kDa
Immunogen:
Residues 1-85 of human Lymphoid Enhancer Factor (LEF)
Isotype:
IgG1
Background Information:
Monoclonal Anti-LEF-1 (β-Catenin Binding Domain) recognizes the β-Catenin Binding Domain of human LEF-1 using immunoblotting (55-57 kDa). Lymphoid enhancer factor, LEF-1, is a transcription factor of the High Mobility Group of DNA binding proteins. It is one member of a family of four proteins referred to as LEF/TCF transcription factors (LEF-1, TCF-1, TCF-3 and TCF-4). These factors play crucial roles in WNT/Wingless signaling, a signal transduction cascade that directs cell differentiation. Aberrant activation of the WNT/Wingless pathway is also a root cause in the genesis of certain cancers such as colon cancer, melanoma and breast cancer. LEF-1 is expressed during development in many different differentiating tissues, and in a few tissues after birth. LEF-1 expression is required for proper development of breast, teeth, hair, whiskers and the trigeminal nerve.
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Species Reactivity:
Human
Mouse
Control:
Jurkat nuclear extract
Quality Assurance:
routinely evaluated by immunoblot on Jurkat cell lysate
Purification Method:
Thiophilic and size exclusion chromatography
Presentation:
100µg of lyophilized thiophilic and size exclusion chromatography purified mouse IgG1 from 1mL 2x PBS / 0.09 % Na-azide / PEG and Sucrose. Reconstitute with 100 µL H2O (15 min, RT) for a final concentration of 1 mg/mL. Store at -20°C.
Storage Conditions:
2 years at -20°C
UniProt Number:
Q9UJU2
Entrez Gene Number:
NM_016269
Gene Symbol:
LEF1
LEF-1
TCF1-alpha
TCF1ALPHA
DKFZp586H0919
View All »
Usage Statement:
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Key Applications:
Western Blotting
Entrez Gene Summary:
Lymphoid enhancer-binding factor-1 (LEF1) is a 48-kD nuclear protein that is expressed in pre-B and T cells. It binds to a functionally important site in the T-cell receptor-alpha (TCRA; MIM 186880) enhancer and confers maximal enhancer activity. LEF1 belongs to a family of regulatory proteins that share homology with high mobility group protein-1 (HMG1; MIM 163905).[supplied by OMIM]
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UniProt Summary:
FUNCTION: SwissProt: Q9UJU2 # Participates in the Wnt signaling pathway. Activates transcription of target genes in the presence of CTNNB1 and EP300. May play a role in hair cell differentiation and follicle morphogenesis. TLE1, TLE2, TLE3 and TLE4 repress transactivation mediated by LEF1 and CTNNB1. Regulates T-cell receptor alpha enhancer function. Binds DNA in a sequence-specific manner. PIAG antagonizes both Wnt-dependent and Wnt-independent activation b -20°C

ABS485   Anti-phospho LEF (Thr155)   品牌 Millipore

Anti-phospho LEF (Thr155)

货号： ABS485      产品名称： Anti-phospho LEF (Thr155)   品牌： Millipore 规格： EA 三周到货 生化实验

Anti-phospho LEF (Thr155)
Species Reactivity Key Applications Host Format Antibody Type
H  WB, PIA Rabbit Affinity Purified Polyclonal Antibody
Description:
Anti-phospho LEF Antibody (Thr155)
Molecular Weight:
~55 kDa observed. Uncharacterized band(s) may be observed in some cell lysates.
Epitope:
Proline Rich Domain
Immunogen:
KLH-conjugated linear peptide corresponding to the Proline Rich Domain of human LEF1 phosphorylated at Thr155.
Background Information:
Lymphoid enhancer factor, LEF-1, is a transcription factor of the High Mobility Group of DNA binding proteins. It is one member of a family of four proteins referred to as LEF/TCF transcription factors (LEF-1, TCF-1, TCF-3, and TCF-4). These factors play crucial roles in WNT/Wingless signaling, a signal transduction cascade that directs cell differentiation. Aberrant activation of the WNT/Wingless pathway is also a root cause in the genesis of certain cancers such as colon cancer, melanoma, and breast cancer. LEF-1 is expressed during development in many different differentiating tissues, and in a few tissues after birth. LEF-1 expression is required for proper development of breast, teeth, hair, whiskers, and the trigeminal nerve.
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Species Reactivity:
Human
Species Reactivity Note:
Predicted to react with Mouse, Rat, Bovine, Chicken, Porcine, Xenopus, and Zebrafish based on 100% sequence homology.
Application Notes:
Western Blotting/Peptide Inhibition Analysis: 0.02 µg/mL from a representative lot detected phospho LEF (Thr155) in 10 µg of Jurkat cell lysate treated with Calyculin A and Okadaic acid.
Quality Assurance:
Evaluated by Western Blotting in Jurkat cell lysate treated with Calyculin A and Okadaic acid
Western Blotting Analysis: 0.02 µg/mL of this antibody detected phospho LEF (Thr155) in 10 µg of Jurkat cell lysate treated with Calyculin A and Okadaic acid.
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Purification Method:
Affinity purified
Presentation:
Purified rabbit polyclonal serum in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Storage Conditions:
Stable for 1 year at 2-8°C from date of receipt.
UniProt Number:
Q9UJU2
Entrez Gene Number:
NP_057353
Gene Symbol:
LEF1
Alternate Names:
Lymphoid enhancer-binding factor 1
LEF-1
T cell-specific transcription factor 1-alpha
TCF1-alpha
TCF1A
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Usage Statement:
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Key Applications:
Western Blotting
Peptide Inhibition Assay
Product Name:
Anti-phospho LEF (Thr155)
Concentration:
Please refer to the Certificate of Analysis for the lot-specific concentration.
Antibody Type:
Polyclonal Antibody
Qty/Pk:
100 μg
Format:
Affinity Purified
Host:
Rabbit
Applications:
Anti-phospho LEF Antibody (Thr155) is a highly specific rabbit polyclonal antibody, that targets LEF-1 (lymphoid Enhancer Factor 1) & has been tested in western blotting & Peptide Inhibition Assay.

2-8℃

17-604   ChIPAb+ Lef1   品牌 Millipore

ChIPAb+ Lef1

货号： 17-604      产品名称： ChIPAb+ Lef1   品牌： Millipore 规格： EA 三周到货 生化实验

ChIPAb+ LEF1 – ChIP Validated Antibody and Primer Set
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REFERENCES
Gbx2 directly restricts Otx2 expression to forebrain and midbrain, competing with class III POU factors.
Fumitaka Inoue,Daisuke Kurokawa,Maiko Takahashi,Shinichi Aizawa (2012) Molecular and cellular biology.32
β-Catenin/LEF1 transactivates the microRNA-371-373 cluster that modulates the Wnt/β-catenin-signaling pathway.
A-D Zhou,L-T Diao,H Xu,Z-D Xiao,J-H Li,H Zhou,L-H Qu (2012) Oncogene.31
Species Reactivity Key Applications Host Format Antibody Type
H, M  WB, ChIP Mouse Purified Monoclonal Antibody
Description:
ChIPAb+ LEF1 – ChIP Validated Antibody and Primer Set
Promotional Text:
Special Shipping Offer on Antibodies
100% Performance Guaranteed
Trade Name:
Upstate (Millipore)
Product Overview:
Every lot of the ChIPAb+ line of antibodies is individually validated for chromatin precipitation, in order to guarantee successful ChIP assays every time. Each antibody includes a control primer set for performance confirmation. LEF1 antibody and the negative control antibody (mouse normal IgG) can be used to demonstrate that the LEF1 antibody is functionally validated in the precipitation of LEF1 associated chromatin.
The qPCR primers included flank the LEF1/TCF binding site in human c-myc promoter.
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Specificity:
Recognizes LEF1 (lymphoid enhancer-binding factor 1).
Molecular Weight:
55-57 kDa
Immunogen:
The antibody is made against amino acid residues 1-85 of human LEF1.
Isotype:
IgG1
Species Reactivity:
Human
Mouse
Application Notes:
Western Blot Analysis:
Jurkat cell lysate was resolved by electrophoresis, transferred to nitrocellulose and probed with anti-LEF1 (1 mg/mL). Proteins were visualized using a goat antimouse secondary antibody conjugated to HRP and a chemiluminescence detection system (Please see figures).
Control:
Includes negative control mouse IgG antibody and control primers specific for human c-myc promoter.
Quality Assurance:
Chromatin Immunoprecipitation:
Sonicated chromatin prepared from 3×106 HT29 cells was subjected to chromatin immunoprecipitation using 4 μg of either the negative control antibody, Mouse Normal IgG or mouse Anti-LEF1 and the Magna ChIP™ G kit (Cat.# 17-611).
Successful enrichment of LEF1 associated DNA fragments was verified by qPCR using ChIP Primers c-myc flanking the human c-myc promoter that contains LEF1 binding sites (Please see figures).
Please refer to the EZ-Magna ChIP™ G (Cat.# 17-409) or EZ-ChIP™ (Cat.# 17-371) kit protocols for experimental details.
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Presentation:
Anti-LEF1 (mouse IgG1). 1 vial containing 100 mg of thiophilic and size exclusion chromatography purified mouse IgG1 in 100 mL of phosphate buffered saline with sodium azide. Store at-20°C. The antibody is made against amino acid residues 1-85 of human LEF1 and can recognize human and mouse LEF1. It does not cross-react with TCF-3 or TCF-4.
Normal Mouse IgG. One vial containing 125 μg of normal mouse IgG in 125 μL volume. Store at -20°C.
ChIP primers c-myc. One vial containing 75 mL of 5 μM of each control primer specific for human c-myc promoter. Store at -20°C.
FOR: CCC AAA AAA AGG CAC GGA A
REV: TAT TGG AAA TGC GGT CAT GC
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Storage Conditions:
1 year at -20°C from date of shipment
UniProt Number:
Q9UJU2
Entrez Gene Number:
NM_016269.2
Gene Symbol:
LEF1
DKFZp586H0919
TCF1ALPHA
LEF-1
TCF1-alpha
View All »
Usage Statement:
Unless otherwise stated in our catalog or other -20℃