ANTI-Phospho-Dnmt1-pS714

货号： 07-1594      产品名称： ANTI-Phospho-Dnmt1-pS714   品牌： Millipore 规格： EA 三周到货 生化实验

Anti- Phospho-Dnmt1(Ser714)
Species Reactivity Key Applications Host Format Antibody Type
H  WB, ELISA, DB Rabbit Purified Polyclonal Antibody
Description:
Anti-Phospho-Dnmt1(Ser714) Antibody
Promotional Text:
Special Shipping Offer on Antibodies
100% Performance Guaranteed
Specificity:
This antibody recognizes human Dnmt1 phosphorylated on Ser714.
Molecular Weight:
~184 kDa
Epitope:
Ser714
Immunogen:
The immunogen was a KLH-conjugated synthetic phosphopeptide corresponding to amino acid residues surrounding Ser714 of human Dnmt1.
Modifications:
Phosphorylation
Background Information:
Methylation of DNA at cytosine residues plays an important role in regulation of gene expression, genomic imprinting and is essential for mammalian development. Hypermethylation of CpG islands in tumor suppressor genes may be linked with development of cancer. Three families of mammalian DNA methyltransferase genes have been identified which include Dnmt1, Dnmt2 and Dnmt3. Dnmt1 is constitutively expressed in proliferating cells and inactivation of Dnmt1 causes global demethylation of genomic DNA and embryonic lethality. Dnmt1 co-purifies with the retinoblastoma (Rb) tumour suppressor gene product, E2F1, and HDAC1. Dnmt1 also cooperates with Rb to repress transcription from promoters containing E2F binding sites suggesting a link between DNA methylation, histone deacetylase and sequence-specific DNA binding activity, as well as a growth-regulatory pathway that is disrupted in nearly all cancer cells. Phosphorylation of Ser714 is induced by EGF, but the role of this modification is unknown.
Species Reactivity:
Human
Species Reactivity Note:
Human. Not tested in other species.
Application Notes:
ELISA:
This antibody has been shown by an outside laboratory to be suitable for ELISA (1:1000).
Dot blotting:
This antibody has been shown by an outside laboratory to be specific for the phospho-Ser714 immunogen peptide, but not the unphosphorylated peptide, by dot blotting (1:500).
Control:
HeLa nuclear extract or EGF-treated whole HeLa cell extract.
Quality Assurance:
Western Blot Analysis:
1:250 dilution of this antibody detected Dnmt1 on 10 µg HeLa nuclear extract
Purification Method:
Purified
Presentation:
Purified rabbit polyclonal in buffer containing 0.09% NaN3.
Storage Conditions:
Stable for 6 months at 2-8°C from date of receipt. For long term storage, store at -20ºC. Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
UniProt Number:
P26358
Entrez Gene Number:
NP_001370
Gene Symbol:
AIM
CXXC9
DNMT
Dnmt1
EC 2.1.1.37
FLJ16293
M.HsaI
MCMT
MGC104992
Alternate Names:
CXXC finger protein 9
CXXC-type zinc finger protein 9
DNA (cytosine-5-)-methyltransferase 1
DNA MTase HsaI
DNA methyltransferase 1
DNA methyltransferase HsaI
Usage Statement:
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Key Applications:
Western Blotting
ELISA
Dot Blot
Entrez Gene Summary:
DNA (cytosine-5-)-methyltrans -20℃