标签： dapi

10236276001-10mg分   DAPI   品牌 Roche

DAPI

货号： 10236276001-10mg分      产品名称： DAPI   品牌： Roche 规格： 10mg分

产品介绍:      
    DAPI即4′,6-二脒基-2-苯基吲哚(4′,6-diamidino-2-phenylindole)﹐是一种能够与DNA中大部分A，T碱基相互结合的荧光染料﹐常用于荧光显微镜观测。因为DAPI可以透过完整的细胞膜﹐它可以用于活细胞和固定细胞的染色。
　　在荧光显微镜观察下，DAPI染剂是利用紫外光波长的光线激发。当DAPI与双股DNA结合时，最大吸收波长为358nm，最大发射波长为461nm，其发散光的波长范围含盖了蓝色至青绿色。DAPI也可以和RNA结合，但产生的荧光强度不及与DNA结合的结果，其发散光的波长范围约在400nm左右。
　　DAPI的发散光为蓝色，且DAPI和绿色荧光蛋白(Green fluorescent protein, GFP)或Texas Red染剂(红色荧光染剂)的发散波长，仅有少部分重叠，研究员可以善用这项特性在单一的样品上进行多重荧光染色。
　　DAPI能快速进入活细胞中与DNA结合，因此DAPI对生物体而言，也被视为一种毒性物质与致癌物。使用过程中应注意操作与抛弃的处理程序。
　　DAPI
　　中文名：4，6-联脒-2-苯基吲哚()
　　英文名：4′,6-diamidino-2-phenylindole，2-(4-amidinophenyl)-1H -indole-6-carboxamidine，DAPI dihydrochloride
　　分子式：C16H15N5
　　分子量：277.324
　　CAS number：28718-90-3
　　光谱性质：与双链DNA结合时最大吸收/最大发射为358 nm/461 nm；与RNA结合时，最大发射移动到400 nm左右。
　　染色原理：
　　DAPI 为一种荧光染料，可以穿透细胞膜与细胞核中的双链DNA结合而发挥标记的作用，可以产生比DAPI自身强20多倍的荧光，和EB相比，对双链DNA的染色灵敏度要高很多倍。显微镜下可以看到显蓝色荧光的细胞，荧光显微镜观察细胞标记的效率高(几乎为100 %) ，且对活细胞无毒副作用。DAPI染色常用于细胞凋亡检测，染色后用荧光显微镜观察或流式细胞仪检测。DAPI也常用于普通的细胞核染色以及某些特定情况下的双链DNA染色。细胞经热激处理后用DAPI染色3分钟，在荧光显微镜下可以看到到细胞核的形态变化。
　　a、 正常的烟草细胞核：核形完整，染色质均匀。
　　b、 染色质固缩，向外周聚集，形成周边化。
　　c、 染色质进一步固缩，形成很多颗粒物质。
　　d、 细胞核破裂形成碎片，核解体。
　　染色步骤：
　　在细胞移植前，将DAPI 以一定的浓度加入培养基中，与细胞共同孵育过夜，用PBS 缓冲液漂洗至少6 次以洗掉未结合的DAPI，在用酶消化后离心收集细胞，加入DMEM 培养基制成细胞悬液以备用。
　　存储条件：-20℃避光保存
　　注意事项：
　　1、DAPI强烈致癌，操作时要戴上手套。
　　2、贮存液用70%酒精配制，浓度100 µg/ml，可用黑纸包住，长期冻存。使用液按1:1000用PBS稀释，最终浓度100 ng/ml。
　　3、DAPI是非常优秀的DNA染料，固定过和没有固定过的活细胞均可用DAPI染色。
Roche相关产品信息
Commercial Product Name:  DAPI  4′,6-Diamidine-2′-phenylindole dihydrochloride
Mat.-No./ Genisys-No.: 10236276001
CAS-No.: 28718-90-3
EINECS-No.: 249-186-7
Molecular formula: C16H17Cl2N5
Molecular Weight: 350.3
Form: solid
Colour: white or yellowish
pH: 4 – 5 10 g/l at 20 °C
Application:DAPI is a fluorescent dye that specifically stains double-stranded DNA and is used for the fluorescence-microscopic detection of mycoplasma in cell culture. Contents:Powder, crystallized, non-sterile
Purity:>90% (from N)

2-8°C

H-1200   现货|VECTASHIELD with DAPI|防荧光萃灭(含DAPI)   品牌 Vector

现货|VECTASHIELD with DAPI|防荧光萃灭(含DAPI)

货号： H-1200      产品名称： 现货|VECTASHIELD with DAPI|防荧光萃灭(含DAPI)   品牌： Vector 规格： 10 ml 现货

H-1200   现货|VECTASHIELD with DAPI|防荧光萃灭(含DAPI)   品牌 Vector

 VECTASHIELD Mounting Medium with DAPI /防荧光萃灭；含DAPI,可衬染细胞核
VECTASHIELD® Mounting Media are unsurpassed in preventing photobleaching. The different formulations of VECTASHIELD® Mounting Media all offer the same outstanding anti-fade and anti-photobleaching properties. They are all compatible with fluorescein, Texas Red®, AMCA, DyLight® dyes, Alexa Fluor® dyes, fluorescent nuclear stains, fluorescent proteins, fluorescent tracers, histochemical stains, and most fluorochromes.
Features of VECTASHIELD® Mounting Media:
Inhibit photobleaching of most fluorochromes
Available in non-hardening or hardening formulations
Available with or without DAPI or propidium iodide
No warming necessary
Slides can be viewed after prolonged storage
Continues to inhibit photobleaching after prolonged storage of mounted slides
Easy to use
Optically clear
Ideal refractive index
The original VECTASHIELD® Mounting Medium is a glycerol-based, aqueous mountant that does not solidify but remains a viscous liquid on the slide. After mounting, coverslipped slides will not readily dry out and can be reviewed for weeks afterwards without sealing. For prolonged storage, coverslips can be permanently sealed around the perimeter with nail polish. Mounted slides should be stored at 4 ºC.
Both the VECTASHIELD® HardSet™ and the original VECTASHIELD® Mounting Media are available with or without the counterstain DAPI (4’, 6-diamidino-2-phenylindole). DAPI produces a blue fluorescence when bound to DNA with excitation at about 360 nm and emission at 460 nm.
VECTASHIELD® Mounting Media are compatible with a wide array of fluorochromes, enzymatic substrates, and fluorescent proteins. Please consult the compatibility table (located in product information box) to determine if VECTASHIELD® will be compatible in your system.
Please note: VECTASHIELD® Mounting Medium may not be compatible with all enzymatic substrates or fluorescent proteins and test sections would be advisable when using anything other than commonly used fluorochromes.
The refractive index for VECTASHIELD® Mounting Medium is 1.44.
1R.J. Florijn, et. al. Cytometry, 19 (1995) 177-182.

1214-20   FluorLastTM with DAPI   品牌 Biovision

FluorLastTM with DAPI

货号： 1214-20      产品名称： FluorLastTM with DAPI   品牌： Biovision 规格： 20 ml 3周到货 生化实验

FluorLast™ with DAPI
Product Overview
Product Highlights:
• Ready-to-use mounting medium
• Applications: Immunofluorescence, confocal microscopy
Description: BioVision’s FluorLast™ with DAPI is an aqueous mounting medium for preserving fluorescence of tissue and cell smears. This unique formula prevents rapid photobleaching of FITC, Texas Red, AMCA, Cy2, Cy3, Cy5, Alexa fluoro 488, Alexa fluoro 594, Green fluorescent protein (GFP), tetramethyly rhodamine, Redox. Phycoerythrin (RP-E), Phyocyanin (PC), and Allophycocyanin (APC). Fluorescence is retained during prolonged storage at 4°C in the dark. This medium does not contain phenylenediamine, which destroys immunofluorescence of Cy dyes, RP-E, PC and APC. This mounting medium is fortified with DAPI, which is a counter-stain for DNA and can be used for in situ hybridization techniques or other methods where fluorescence of DNA staining is required. DAPI excites at 360nm and emits at 460nm, producing a blue fluorescence. RNA is also stained with DAPI.
Storage Temp.: +4°C
Shipping: gel pack
Usage: For Research Use Only! Not For Use in Humans.

+4°C

1218-20   FluorLastTM with DAPI and DABCO   品牌 Biovision

FluorLastTM with DAPI and DABCO

货号： 1218-20      产品名称： FluorLastTM with DAPI and DABCO   品牌： Biovision 规格： 20 ml 3周到货 生化实验

FluorLast™ with DAPI and DABCO
Product Overview
Product Highlights:
• Ready-to-use mounting medium
• Applications: Immunofluorescence, confocal microscopy
Description: BioVision’s FluorLast™ with DAPI & anti-fading agent 1, 4-Diazobicyclo-(2,2,2-octane (DABCO™) is an aqueous mounting medium for preserving fluorescence of tissue and cell smears. This unique formula prevents rapid photobleaching of FITC, Texas Red, AMCA, Alexa fluoro 488, Alexa fluoro 594, Cy dyes, tetramethyly rhodamine, and Redox. The fluorescence is retained during prolonged storage at 4°C in the dark. This mounting medium is fortified with DAPI, which is a counter-stain for DNA and can be used for in situ hybridization techniques or other methods where fluorescence of DNA staining is required. DAPI excites at 360nm and emits at 460nm, producing a blue fluorescence. RNA is also stained with DAPI.
Storage Temp.: +4°C
Shipping: gel pack
Usage: For Research Use Only! Not For Use in Humans.

+4°C

20004   FluoroQuest Mounting Medium with DAPI   品牌 AAT

FluoroQuest Mounting Medium with DAPI

货号： 20004      产品名称： FluoroQuest Mounting Medium with DAPI   品牌： AAT 规格： 1 kit 二周到货 生化试验

FluoroQuest� Mounting Medium with DAPI
Cat # 20004
Storage F/D/L (see storage codes below)
Unit Size 1 kit
Ex (nm) 358
Em (nm) 461  
Solvent Water
FluoroQuest� with DAPI is an aqueous mounting medium for preserving fluorescence of tissue and cell smears. This mounting medium is fortified with DAPI which is a counterstain for DNA. For use in in situ hybridization techniques or other methods where fluorescence of DNA staining is required. DAPI excites at 360 nm and emits at 460 nm, producing a blue fluorescence. RNA is also stained with DAPI. 
Detailed Information:   Protocol      References      MSDS      Spectrum
Related Products:     Fuorescence Imaging 
Storage Codes:
  D = Desiccated
  L = Avoid light
  F = Freeze (<-15 癈)
  R = Refrigerated (2-4 癈)
  RT = Room temperature (4-50 癈)