标签归档：dap

tlrl-c12dap   C12-iE-DAP   品牌 Invivogen

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C12-iE-DAP

货号： tlrl-c12dap      产品名称： C12-iE-DAP   品牌： Invivogen 规格： 1 mg 二周到货 生化试验

C12-iE-DAP
Acylated derivative of iE-DAP
C12-iE-DAP is an acylated derivative of the dipeptide iE-DAP (γ-D-Glu-mDAP), present in the peptidoglycan (PGN) of Gram-negative bacilli and particular Gram-positive bacteria. C12-iE-DAP is recognized by the intracellular sensor NOD1, which results in NF-κB activation and the production of inflammatory cytokines. C12-iE-DAP stimulates NOD1 at concentrations 100- to 1000-fold lower than iE-DAP. C12-iE-DAP provided by InvivoGen is chemically synthesized and tested using HEK-Blue™ NOD1.
Note: C12-iE-DAP is a mixture of Lauroyl-g-D-Glu-D-mDAP and Lauroyl-g-D-Glu-L-mDAP.
C12-iE-DAP is also available on ready-to-use 8-well strips pre-coated at 7 different concentrations and a negative control.
Specificity: NOD1 agonist
Working concentration: 1 ng – 1 μg/ml
Endotoxin level: EndoFit™ (<0.001 EU/μg)
Solubility: 10 mg/ml DMSO or methanol
Synonym: Lauroyl-γ-D-glutamyl-meso-diaminopimelic acid
Formula: C24H43N3O8
Molecular weight: 501.61

 

tlrl-dap   iE-DAP   品牌 Invivogen

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iE-DAP

货号： tlrl-dap      产品名称： iE-DAP   品牌： Invivogen 规格： 5 mg 二周到货 生化试验

iE-DAP
γ-D-Glu-mDAP (iE-DAP) is a dipeptide present in the peptidoglycan (PGN) of all Gram-negative and certain Gram-positive bacteria. iE-DAP is the minimal motif recognized by the intracellular receptor NOD1. Recognition of iE-DAP by NOD1 induces NF-κB activation and the production of inflammatory cytokines. iE-DAP provided by InvivoGen is chemically synthesized and tested using HEK-Blue™ NOD1.
Note: iE-DAP is a mixture of γ-D-Glu-D-mDAP and γ-D-Glu-L-mDAP.
Specificity: NOD1 agonist
Working Concentration: 1 – 100 µg/ml
Endotoxin level:  <0.125 EU/ml
Solubility: 10 mg/ml in water
Formula: C12H21N3O7
Molecular weight: 319.31
Synonym: γ-D-glutamyl-meso-diaminopimelic acid

tlrl-tdap   Tri-DAP   品牌 Invivogen

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Tri-DAP

货号： tlrl-tdap      产品名称： Tri-DAP   品牌： Invivogen 规格： 1 mg 三周到货 生化试验

Tri-DAP
L-Ala-γ-D-Glu-mDAP (Tri-DAP) comprises the iE-DAP (γ-D-Glu-mDAP) dipeptide and a L-Ala residue. Tri-DAP is present in the peptidoglycan (PGN) of Gram-negative bacilli and certain Gram-positive bacteria. Tri-DAP is recognized by the intracellular sensor NOD1, which induces a signaling cascade leading to NF-κB activation and the production of inflammatory cytokines. Tri-DAP exhibits a ~3-fold higher ability to activate NF-κB than iE-DAP. Tri-DAP provided by InvivoGen is chemically synthesized and tested using HEK-Blue™ NOD1.
Note: Tri-DAP is a mixture of L-Ala-γ-D-Glu-D-mDAP and L- Ala-γ-D-Glu-L-mDAP.
Specificity: NOD1 agonist
Working Concentration: 100 ng – 10 µg/ml
Endotoxin level:  <0.125 EU/ml
Solubility: 10 mg/ml in water
Formula: C15H26N4O8
Molecular weight: 390.39
Synonym: L-alanyl-γ-D-glutamyl-meso-diaminopimelic acid

06-859   ANTI-DAP KINASE *PO*   品牌 Millipore

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ANTI-DAP KINASE *PO*

货号： 06-859      产品名称： ANTI-DAP KINASE *PO*   品牌： Millipore 规格： EA 3周到货 生化实验

Anti-DAPK Antibody
Close
REFERENCES
DAP kinase links the control of apoptosis to metastasis.
Inbal, B, et al. (1997) Nature, 390: 180-4 (1997)
DAP-kinase is a Ca2+/calmodulin-dependent, cytoskeletal-associated protein kinase, with cell death-inducing functions that depend on its catalytic activity.
Cohen, O, et al. (1997) EMBO J., 16: 998-1008 (1997)
Identification of a novel serine/threonine kinase and a novel 15-kD protein as potential mediators of the gamma interferon-induced cell death.
Deiss, L P, et al. (1995) Genes Dev., 9: 15-30 (1995)
Species Reactivity Key Applications Host Format Antibody Type
H  WB Rabbit Purified Polyclonal Antibody
Description:
Anti-DAPK Antibody
Promotional Text:
Special Shipping Offer on Antibodies
100% Performance Guaranteed
Trade Name:
Upstate (Millipore)
Specificity:
Recognizes DAP kinase at approximately 130 kDa and occasionally a related band at 66 kDa (based on competition with peptide immunogen) and a non-specific band at 220 kDa.
Molecular Weight:
130kDa
Immunogen:
KLH-conjugated, synthetic peptide corresponding to amino acids 710-727 of human DAP kinase [C-RRRRPRLSSTNSSRFPPS]
Isotype:
IgG
Background Information:
Death Associated Protein Kinase (DAP kinase) is a Ca2+/calmodulin-dependent, cytoskeletal associated protein kinase whose expression is implicated in the sensitivity of cells to apoptotic effects of TNF-a and interferon-g. Deletion of the calmodulin-binding domain resulted in a constitutively active mutant with a more potent cytotoxic effect. In contrast, the catalytically inactive mutant exerted a dominant negative effect, reducing cytotoxicity and protecting cells from interferon-γ induced cell death. DAP Kinase expression is frequently lost in human carcinomas and B-cell leukemia, and lower levels of expression correlates with high rates of metastasis.
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Species Reactivity:
Human
Quality Assurance:
Immunoblot Analysis: 1-2 μg/ml of this lot detected DAP kinase in whole cell lysates from SK-N-MC cells.
Purification Method:
Protein A chromatography
Presentation:
0.07M Tris-glycine, pH 7.4, 0.105 M NaCl, 0.035% sodium azide and 30% glycerol.
Storage Conditions:
2 years at -20°C
UniProt Number:
P53355
Entrez Gene Number:
NM_004938
Gene Symbol:
DAPK1
DAPK
DKFZp781I035
Usage Statement:
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Key Applications:
Western Blotting
Entrez Gene Summary:
Death-associated protein kinase 1 is a positive mediator of gamma-interferon induced programmed cell death. DAPK1 encodes a structurally unique 160-kD calmodulin dependent serine-threonine kinase that carries 8 ankyrin repeats and 2 putative P-loop consensus sites. It is a tumor suppressor candidate.
UniProt Summary:
FUNCTION: SwissProt: P53355 # Calcium/calmodulin-dependent serine/threonine kinase which acts as a positive regulator of apoptosis.
COFACTOR: Magnesium.
SIZE: 1432 amino acids; 160286 Da
SUBCELLULAR LOCATION: Cytoplasm. Note=Colocalizes with the actin filament system.
SIMILARITY: SwissProt: P53355 ## Belongs to the protein kinase superfamily. CAMK Ser/Thr protein kinase family. DAP kinase subfamily. & Contains 10 ANK repeats. & Contains 1 death domain. -20°C

5110   FMOC-Dap(Boc)-OH   品牌 AAT

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FMOC-Dap(Boc)-OH

货号： 5110      产品名称： FMOC-Dap(Boc)-OH   品牌： AAT 规格： 10 g 二周到货 生化试验

FMOC-Dap(Boc)-OH
Cat # 5110
Storage F (see storage codes below)
Unit Size 10 g
MW 426.46
Solvent DMF
FMOC-Dap(Boc)-OH is a common peptide building block. As lysine residue its 3-amino group is often used for carrying a tag moiety such as a fluorescent dye, a quencher or biotin.
Detailed Information:   Protocol      References      MSDS
Related Products:     Boc Amino Acids     Fmoc Amino Acids 
Storage Codes:
  D = Desiccated
  L = Avoid light
  F = Freeze (<-15 癈)
  R = Refrigerated (2-4 癈)
  RT = Room temperature (4-50 癈)

AB10520   Anti-DAP-5   品牌 Millipore

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Anti-DAP-5

货号： AB10520      产品名称： Anti-DAP-5   品牌： Millipore 规格： EA 三周到货 生化实验

Anti-DAP-5
Species Reactivity Key Applications Host Format Antibody Type
H, M  WB Rabbit Serum Polyclonal Antibody
Description:
Anti-DAP-5 Antibody
Promotional Text:
Special Shipping Offer on Antibodies
100% Performance Guaranteed
Specificity:
This antibody recognizes DAP-5.
Molecular Weight:
97 kDa
Epitope:
Unknown
Immunogen:
KLH-conjugated linear peptide corresponding to human DAP-5.
Isotype:
IgG
Background Information:
Death Associated Protein 5 (DAP-5) is a protein with high amino acid sequence homology to Eukaryotic Translation Initiation Factor 4G (eIF4G). Compared with eIF4G, DAP5 lacks the N-terminal region necessary for cap-dependent translation and has a unique C-terminal part functioning as a regulator for interferon-gamma induced cell death. DAP5 is cleaved at Asp790 during apoptosis . The C-terminal truncated form of DAP5 functions as a cap-independent translation initiation factor responsible for the mediation of its own translation during apoptosis.
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Species Reactivity:
Human
Mouse
Species Reactivity Note:
Proven to react with human and mouse. Predicted to react with rat based on 100% sequence homology.
Control:
Jurkat cell lysate
Quality Assurance:
Evaluated by Western Blot on Jurkat cell lysate.
Western Blot Analysis: 1:1,000 dilution of this antibody detected DAP-5 on 10 µg of jurkat cell lysate.
Purification Method:
Unpurified
Presentation:
Rabbit polyclonal serum containing 0.05% sodium azide.
Storage Conditions:
Stable for 1 year at -20°C from date of receipt. Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
UniProt Number:
P78344
Entrez Gene Number:
NP_001036024.1
Gene Symbol:
EIF4G2
AAG1
DAP-5
DAP5
NAT1
p97
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Alternate Names:
Death-associated protein 5
aging-associated protein 1
eIF-4-gamma 2
eIF-4G 2
eIF4G 2
eukaryotic translation initiation factor 4 gamma, 2
eukaryotic translation initiation factor 4G-like 1
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Usage Statement:
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Key Applications:
Western Blotting
Entrez Gene Summary:
Translation initiation is mediated by specific recognition of the cap structure by eukaryotic translation initiation factor 4F (eIF4F), which is a cap binding protein complex that consists of three subunits: eIF4A, eIF4E and eIF4G. The protein encoded by this gene shares similarity with the C-terminal region of eIF4G that contains the binding sites for eIF4A and eIF3; eIF4G, in addition, contains a binding site for eIF4E at the N-terminus. Unlike eIF4G, which supports cap-dependent and independent translation, this gene product functions as a general repressor of translation by forming translationally inactive complexes. In vitro and in vivo studies indicate that translation of this mRNA initiates exclusively at a non-AUG (GUG) codon. Alternatively spliced transcript variants encoding different isoforms of this gene have been described. [provided by RefSeq]
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UniProt Summary:< -20℃

5570R-100   DAP12 Antibody   品牌 Biovision

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DAP12 Antibody

货号： 5570R-100      产品名称： DAP12 Antibody   品牌： Biovision 规格： 100 μg 3周到货 生化实验

DAP12 Antibody
Product Overview
Antibody Target: DAP12
Alternative Name: DAP-12, DAP 12, DAP12, TYRO protein tyrosine kinase binding protein , TYROBP
Host: Rabbit
Antibody Type: Polyclonal
Isotype: Rabbit IgG
Immunogen: Synthetic peptide corresponding to residues surrounding amino acids 94 of mouse DAP-12
Accession#: AAD09438
Gene ID: 22177
Appearance: Colorless liquid
Concentration: 0.5 mg/ml
Formulation: 100 µg (0.5 mg/ml) affinity purified rabbit anti-DAP12 polyclonal antibody in phosphate buffered saline (PBS), pH 7.2, containing 30% glycerol, 0.5% BSA, 0.01% thimerosal.
Purification: Affinity purified
Species Reactivity: mouse, rat
Application: Western blot
Application & Usage: Western blot analysis (0.5-4 µg/ml). However, the optimal conditions should be determined individually.
Storage Temp.: -20 °C
Shipping: gel pack
Background Descriptions: DAP12 (KARAP) is a natural killer (NK) cell-expressed cell surface receptor and belongs to the immunoglobulin and C-type lectin superfamily. Unlike other members of this family which possess immunoreceptor tyrosine-based inhibitory motifs (ITIM), DAP12 contains an immunoreceptor tyrosine-based activation motif (ITAM) in its cytoplasmic domain and is present as a disulphide-bonded homodimer. Crosslinking of DAP 12-KIR complexes (membrane glycoproteins of the killer-cell inhibitory receptor family without an ITIM in their cytoplasmic domain) results in cellular activation.
Handling: The antibody solution should be gently mixed before use.
Usage: For Research Use Only! Not to be used in humans.

-20°C

5570RBP-50   DAP12 Blocking Peptide   品牌 Biovision

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DAP12 Blocking Peptide

货号： 5570RBP-50      产品名称： DAP12 Blocking Peptide   品牌： Biovision 规格： 50 μg 3周到货 生化实验

DAP12 Blocking Peptide
Product Name: DAP12 Blocking Peptide
Antibody Target: Synaptotagmin
Accession #: P21707
Gene ID: 25716
Appearance: Colorless liquid
Concentration: 0.5mg/ml
Formulation: 50 µg (0.5 mg/ml) in phosphate buffered saline (PBS), pH 7.2, containing 50% glycerol, 1% BSA and 0.02% thimerosal.
Storage Temp.: -20°C
Shipping: Gel Pack
Application & Usage: The peptide is used for blocking the antibody activity of synaptotagmin (Cat. # 3177-100). It usually blocks the antibody activity completely in Western blot analysis by incubating the peptide with equal volume of antibody for 30-60 minutes at 37°C.
Description: 50 ug blocking peptide for synaptotagmin polyclonal Antibody (Cat. # 3177-100).
Handling: The peptide solution should be gently mixed before use
USAGE: For Research Use Only! Not For Use in Humans.

-20°C

AB3890   DAP12, RB X HU, 100UG   品牌 Millipore

发表于由

DAP12, RB X HU, 100UG

货号： AB3890      产品名称： DAP12, RB X HU, 100UG   品牌： Millipore 规格： EA 三周到货 生化实验

Anti-DAP12 Antibody
Species Reactivity Key Applications Host Format Antibody Type
H  WB Rabbit Purified Polyclonal Antibody
Description:
Anti-DAP12 Antibody
Promotional Text:
Special Shipping Offer on Antibodies
100% Performance Guaranteed
Trade Name:
Chemicon (Millipore)
Specificity:
Recognizes human DAP12. This antibody detects a band of approximately 12 kDa in Western blot analysis of U937 cell lysate.
Immunogen:
Synthetic peptide corresponding to amino acid residues 89-113 of human DAP12 (Accession # AAD09437).
Background Information:
Natural killer (NK) cells are lymphocytes that function as a component of innate immunity against intracellular and parasitic pathogens, and possibly tumors. DAP12 is expressed on the surface of NK cells as a disulfide-linked homodimer and contains an immunoreceptor tyrosine based activation motif (ITAM) in its cytoplasmic domain. DAP12 non-covalently associates with members of the killer cell immunoglobulin-like receptor (KIR) family resulting in cellular activation.
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Species Reactivity:
Human
Application Notes:
Western blot: 1.0 μg/mL
Optimal working dilutions must be determined by the end user.
Control:
Western blot positive control: U937 cell line
Presentation:
Protein A purified immunoglobulin. Liquid in 0.02M Phosphate Buffer, 0.25M NaCl, with 0.1% sodium azide.
Storage Conditions:
Maintain at 2-8°C for up to 12 months from date of receipt.
UniProt Number:
O43914
Entrez Gene Number:
NM_003332.2
NM_198125.1
Gene Symbol:
TYROBP
DAP12
PLO-SL
KARAP
PLOSL
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Usage Statement:
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
View All »
Key Applications:
Western Blotting
Entrez Gene Summary:
This gene encodes a transmembrane signaling polypeptide which contains an immunoreceptor tyrosine-based activation motif (ITAM) in its cytoplasmic domain. The encoded protein may associate with the killer-cell inhibitory receptor (KIR) family of membrane glycoproteins and may act as an activating signal transduction element. This protein may bind zeta-chain (TCR) associated protein kinase 70kDa (ZAP-70) and spleen tyrosine kinase (SYK) and play a role in signal transduction, bone modeling, brain myelination, and inflammation. Mutations within this gene have been associated with polycystic lipomembranous osteodysplasia with sclerosing leukoencephalopathy (PLOSL), also known as Nasu-Hakola disease. Its putative receptor, triggering receptor expressed on myeloid cells 2 (TREM2), also causes PLOSL. Two alternative transcript variants encoding distinct isoforms have been identified for this gene. Other alternative splice variants have been described, but their full-length nature has not been deterimined.
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UniProt Summary:
FUNCTION: SwissProt: O43914 # Non-covalently associates with membrane glycoproteins of the killer-cell inhibitory receptor (KIR) family without an ITIM in their cytoplasmic domain. Cross-linking of KIR-TYROBP complexes results in cellular activation.
SIZE: 113 amino acids; 12179 Da
SUBUNIT: Homodimer; disulfide-linked. Interacts with SIRPB1 and TREM1. Interacts with CLECSF5. Interacts with SIGLEC14.
SUBCELLULAR LOCATION: Membrane; Single-pass type I membrane protein.
TISSUE SPECIFICITY: Expressed 2-8℃

AB4070   DAP12, RB X HU, 100 UG   品牌 Millipore

发表于由

DAP12, RB X HU, 100 UG

货号： AB4070      产品名称： DAP12, RB X HU, 100 UG   品牌： Millipore 规格： EA 三周到货 生化实验

Anti-DAP12 Antibody
Species Reactivity Key Applications Host Format Antibody Type
H, M  IP Rabbit Purified Polyclonal Antibody
Description:
Anti-DAP12 Antibody
Promotional Text:
Special Shipping Offer on Antibodies
100% Performance Guaranteed
Trade Name:
Chemicon (Millipore)
Specificity:
Recognizes DAP12 protein.
Immunogen:
Recombinant DAP12 protein.
Background Information:
DAP12 is a transmembrane adaptor protein that is non-covalently associated with several cell surface receptors on natural killer (NK), myeloid, and presumably neuronal cells (Bakker, 2000, Campbell, 1999). DAP12 contains an ITAM domain that preferentially recruits the protein tyrosine kinase Syk to initiate signal transduction (McVicar, 1998). Deficiency of DAP12 results in reduced antigen presentation function by myeloid cells and defects in function of certain NK cell receptors in mice (Bakker. 2000, Tomasello, 2000), as well as presenile dementia and bone cysts in humans (Paloneva, 2000).
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Species Reactivity:
Human
Mouse
Application Notes:
Immunoprecipitation (2-4 μg/mg of protein lysate)
Optimal working dilutions must be determined by end user.
Control:
POSITIVE CONTROL: NKp44 positive cells.
Presentation:
Purified immunoglobulin. Liquid in phosphate buffered saline with 0.08% sodium azide.
Storage Conditions:
Maintain at -20°C in undiluted aliquots for up to 12 months from date of receipt. Avoid repeated freeze/thaw cycles.
UniProt Number:
O43914
Entrez Gene Number:
NM_003332.2
NM_198125.1
Gene Symbol:
TYROBP
DAP12
PLO-SL
KARAP
PLOSL
View All »
Usage Statement:
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
View All »
Key Applications:
Immunoprecipitation
Entrez Gene Summary:
This gene encodes a transmembrane signaling polypeptide which contains an immunoreceptor tyrosine-based activation motif (ITAM) in its cytoplasmic domain. The encoded protein may associate with the killer-cell inhibitory receptor (KIR) family of membrane glycoproteins and may act as an activating signal transduction element. This protein may bind zeta-chain (TCR) associated protein kinase 70kDa (ZAP-70) and spleen tyrosine kinase (SYK) and play a role in signal transduction, bone modeling, brain myelination, and inflammation. Mutations within this gene have been associated with polycystic lipomembranous osteodysplasia with sclerosing leukoencephalopathy (PLOSL), also known as Nasu-Hakola disease. Its putative receptor, triggering receptor expressed on myeloid cells 2 (TREM2), also causes PLOSL. Two alternative transcript variants encoding distinct isoforms have been identified for this gene. Other alternative splice variants have been described, but their full-length nature has not been deterimined.
View All »
UniProt Summary:
FUNCTION: SwissProt: O43914 # Non-covalently associates with membrane glycoproteins of the killer-cell inhibitory receptor (KIR) family without an ITIM in their cytoplasmic domain. Cross-linking of KIR-TYROBP complexes results in cellular activation.
SIZE: 113 amino acids; 12179 Da
SUBUNIT: Homodimer; disulfide-linked. Interacts with SIRPB1 and TREM1. Interacts with CLECSF5. Interacts with SIGLEC14.
SUBCELLULAR LOCATION: Membrane; Single-pass type I membrane protein.
TI -20℃