Product Specification Formula:C27H41NO2 MW:411.6 CAS:4449-51-8 Purity:≥98% (TLC) Appearance:white to off-white powder Hazard:HARMFUL. MAY BE TERATOGENIC. Solubility:Soluble in 100% ethanol (20mg/ml), DMSO (4mg/ml), dimethyl formamide (10mg/ml), methanol (7mg/ml). Insoluble in water. Long Term Storage:-20°C Use/Stability:Store, as supplied, at -20°C for up to 1 year. Store solutions at -20°C for up to 3 months. Handling:Protect from light.
Product Specification Formula:C27H41NO2 MW:411.6 CAS:4449-51-8 Purity:≥98% (TLC) Appearance:white to off-white powder Hazard:HARMFUL. MAY BE TERATOGENIC. Solubility:Soluble in 100% ethanol (20mg/ml), DMSO (4mg/ml), dimethyl formamide (10mg/ml), methanol (7mg/ml). Insoluble in water. Long Term Storage:-20°C Use/Stability:Store, as supplied, at -20°C for up to 1 year. Store solutions at -20°C for up to 3 months. Handling:Protect from light.
货号： C-8700-1 g 产品名称： Cyclopamine, Free Base 品牌： LC 规格： 1 g
C-8700 Cyclopamine, Free Base, 99%[11-Deoxyjervine] M.W. 411.62 C27H41NO2 [4449-51-8] M.I. 14: 10201 SPECIAL HAZARD: TERATOGEN. Gloves and mask should be worn when using this compound. Care must be taken to prevent contact through all routes of exposure. Women of childbearing age should be extremely careful in handling cyclopamine! Storage: Store at or below -20 ºC. Solubility: See below. Disposal: A •Cyclopamine blocks activation of the Hedgehog response pathway associated with mutations that either activate the proto-oncogene Smoothened (Smo) or inactivate the tumour suppressor Patched (Ptch) both of which encode multipass transmembrane proteins. Furthermore, it has been demonstrated that the inhibitory effect of cyclopamine results from its direct binding to the Smo heptahelical protein bundle. Taipale, J., et al. “Effects of oncogenic mutations in Smoothened and Patched can be reversed by cyclopamine.” Nature 406: 944-945 (2000). Chen, J.K., et al. “Inhibition of Hedgehog signaling by direct binding of cyclopamine to Smoothened.” Genes & Dev. 16: 2743-2748 (2002). •Induces chick embryo malformations associated with the interruption of Sonic hedgehog (Shh)-mediated dorsoventral patterning. Incardona, J.P., et al. “The teratogenic Veratrum alkaloid cyclopamine inhibits sonic hedgehog signal transduction.” Development. 125: 3553-3562 (1998). •Displays anti-tumor properties. It has been shown to induce apoptosis in both colorectal adenoma- and carcinoma-derived cell lines and in skin basal cell carcinomas. Qualtrough, D., et al. “Hedgehog signalling in colorectal tumour cells: induction of apoptosis with cyclopamine treatment.” Int J Cancer. 110: 831-837 (2004). Tabs, S., and Avci, O. “Induction of the differentiation and apoptosis of tumor cells in vivo with efficiency and selectivity.” Eur J Dermatol. 14: 96-102 (2004). •Pregnant cattle, goats, or sheep who graze on the corn lily plant, Veratrum californicum, at the 14th day of gestation (for sheep) can give birth to deformed offspring with a characteristic single eye in the middle of the forehead. [For example, view www.cybergoat.com/cyclopia/cyclopia.htm.] This type of birth defect recalls one of the most unforgettable images in all of mythology, namely Homer’s Cyclops (for which cyclopamine was named). If plants containing cyclopamine or a similar compound existed in the Mediterranean area in ancient times, it would then seem likely that the Cyclops was not merely a product of Homer’s imagination but rather was based on known, naturally-occurring birth defects in animals. Alternatively, this type of birth defect might arise from spontaneous mutation, as may have been the case for a kitten recently reported: www.samtsai.com/p131. •CYCLOPAMINE SOLUBILITY: Ethanol is the best cyclopamine solvent for biological use. A 2% solution (20 mg/mL) can be prepared by warming the cyclopamine in ethanol to 50-60 ºC; boiling is neither necessary nor recommended. Upon cooling, the cyclopamine remains in solution at room temperature, even if left for several days. However, storage at -20 ºC is recommended, and at this temperature the cyclopamine will crystallize out of solution. Upon thawing, the mixture will need to be heated again to redissolve the cyclopamine. Methanol and DMSO can also be used as cyclopamine solvents, but solubility in methanol is only about 0.7% (7 mg/mL) at best, and solubility in DMSO is about 0.4% (4 mg/mL). Substantial heating is again needed to achieve solution with methanol or DMSO, but once the cyclopamine is dissolved it will remain in solution if kept at room temperature. Storage in either solvent at the recommended -20 ºC will also cause crystallization, as noted above for ethanol, and reheating is necessary after thawing in order to achieve full dissolution. Methanol is less desirable than the other two solvents because it is the most volatile; merely sta
C-8700 Cyclopamine, Free Base, 99%[11-Deoxyjervine] M.W. 411.62 C27H41NO2 [4449-51-8] M.I. 14: 10201 SPECIAL HAZARD: TERATOGEN. Gloves and mask should be worn when using this compound. Care must be taken to prevent contact through all routes of exposure. Women of childbearing age should be extremely careful in handling cyclopamine! Storage: Store at or below -20 ºC. Solubility: See below. Disposal: A •Cyclopamine blocks activation of the Hedgehog response pathway associated with mutations that either activate the proto-oncogene Smoothened (Smo) or inactivate the tumour suppressor Patched (Ptch) both of which encode multipass transmembrane proteins. Furthermore, it has been demonstrated that the inhibitory effect of cyclopamine results from its direct binding to the Smo heptahelical protein bundle. Taipale, J., et al. “Effects of oncogenic mutations in Smoothened and Patched can be reversed by cyclopamine.” Nature 406: 944-945 (2000). Chen, J.K., et al. “Inhibition of Hedgehog signaling by direct binding of cyclopamine to Smoothened.” Genes & Dev. 16: 2743-2748 (2002). •Induces chick embryo malformations associated with the interruption of Sonic hedgehog (Shh)-mediated dorsoventral patterning. Incardona, J.P., et al. “The teratogenic Veratrum alkaloid cyclopamine inhibits sonic hedgehog signal transduction.” Development. 125: 3553-3562 (1998). •Displays anti-tumor properties. It has been shown to induce apoptosis in both colorectal adenoma- and carcinoma-derived cell lines and in skin basal cell carcinomas. Qualtrough, D., et al. “Hedgehog signalling in colorectal tumour cells: induction of apoptosis with cyclopamine treatment.” Int J Cancer. 110: 831-837 (2004). Tabs, S., and Avci, O. “Induction of the differentiation and apoptosis of tumor cells in vivo with efficiency and selectivity.” Eur J Dermatol. 14: 96-102 (2004). •Pregnant cattle, goats, or sheep who graze on the corn lily plant, Veratrum californicum, at the 14th day of gestation (for sheep) can give birth to deformed offspring with a characteristic single eye in the middle of the forehead. [For example, view www.cybergoat.com/cyclopia/cyclopia.htm.] This type of birth defect recalls one of the most unforgettable images in all of mythology, namely Homer’s Cyclops (for which cyclopamine was named). If plants containing cyclopamine or a similar compound existed in the Mediterranean area in ancient times, it would then seem likely that the Cyclops was not merely a product of Homer’s imagination but rather was based on known, naturally-occurring birth defects in animals. Alternatively, this type of birth defect might arise from spontaneous mutation, as may have been the case for a kitten recently reported: www.samtsai.com/p131. •CYCLOPAMINE SOLUBILITY: Ethanol is the best cyclopamine solvent for biological use. A 2% solution (20 mg/mL) can be prepared by warming the cyclopamine in ethanol to 50-60 ºC; boiling is neither necessary nor recommended. Upon cooling, the cyclopamine remains in solution at room temperature, even if left for several days. However, storage at -20 ºC is recommended, and at this temperature the cyclopamine will crystallize out of solution. Upon thawing, the mixture will need to be heated again to redissolve the cyclopamine. Methanol and DMSO can also be used as cyclopamine solvents, but solubility in methanol is only about 0.7% (7 mg/mL) at best, and solubility in DMSO is about 0.4% (4 mg/mL). Substantial heating is again needed to achieve solution with methanol or DMSO, but once the cyclopamine is dissolved it will remain in solution if kept at room temperature. Storage in either solvent at the recommended -20 ºC will also cause crystallization, as noted above for ethanol, and reheating is necessary after thawing in order to achieve full dissolution. Methanol is less desirable than the other two solvents because it is the most volatile; merely sta
货号： C-8700-2 g 产品名称： Cyclopamine, Free Base 品牌： LC 规格： 2 g
C-8700 Cyclopamine, Free Base, 99%[11-Deoxyjervine] M.W. 411.62 C27H41NO2 [4449-51-8] M.I. 14: 10201 SPECIAL HAZARD: TERATOGEN. Gloves and mask should be worn when using this compound. Care must be taken to prevent contact through all routes of exposure. Women of childbearing age should be extremely careful in handling cyclopamine! Storage: Store at or below -20 ºC. Solubility: See below. Disposal: A •Cyclopamine blocks activation of the Hedgehog response pathway associated with mutations that either activate the proto-oncogene Smoothened (Smo) or inactivate the tumour suppressor Patched (Ptch) both of which encode multipass transmembrane proteins. Furthermore, it has been demonstrated that the inhibitory effect of cyclopamine results from its direct binding to the Smo heptahelical protein bundle. Taipale, J., et al. “Effects of oncogenic mutations in Smoothened and Patched can be reversed by cyclopamine.” Nature 406: 944-945 (2000). Chen, J.K., et al. “Inhibition of Hedgehog signaling by direct binding of cyclopamine to Smoothened.” Genes & Dev. 16: 2743-2748 (2002). •Induces chick embryo malformations associated with the interruption of Sonic hedgehog (Shh)-mediated dorsoventral patterning. Incardona, J.P., et al. “The teratogenic Veratrum alkaloid cyclopamine inhibits sonic hedgehog signal transduction.” Development. 125: 3553-3562 (1998). •Displays anti-tumor properties. It has been shown to induce apoptosis in both colorectal adenoma- and carcinoma-derived cell lines and in skin basal cell carcinomas. Qualtrough, D., et al. “Hedgehog signalling in colorectal tumour cells: induction of apoptosis with cyclopamine treatment.” Int J Cancer. 110: 831-837 (2004). Tabs, S., and Avci, O. “Induction of the differentiation and apoptosis of tumor cells in vivo with efficiency and selectivity.” Eur J Dermatol. 14: 96-102 (2004). •Pregnant cattle, goats, or sheep who graze on the corn lily plant, Veratrum californicum, at the 14th day of gestation (for sheep) can give birth to deformed offspring with a characteristic single eye in the middle of the forehead. [For example, view www.cybergoat.com/cyclopia/cyclopia.htm.] This type of birth defect recalls one of the most unforgettable images in all of mythology, namely Homer’s Cyclops (for which cyclopamine was named). If plants containing cyclopamine or a similar compound existed in the Mediterranean area in ancient times, it would then seem likely that the Cyclops was not merely a product of Homer’s imagination but rather was based on known, naturally-occurring birth defects in animals. Alternatively, this type of birth defect might arise from spontaneous mutation, as may have been the case for a kitten recently reported: www.samtsai.com/p131. •CYCLOPAMINE SOLUBILITY: Ethanol is the best cyclopamine solvent for biological use. A 2% solution (20 mg/mL) can be prepared by warming the cyclopamine in ethanol to 50-60 ºC; boiling is neither necessary nor recommended. Upon cooling, the cyclopamine remains in solution at room temperature, even if left for several days. However, storage at -20 ºC is recommended, and at this temperature the cyclopamine will crystallize out of solution. Upon thawing, the mixture will need to be heated again to redissolve the cyclopamine. Methanol and DMSO can also be used as cyclopamine solvents, but solubility in methanol is only about 0.7% (7 mg/mL) at best, and solubility in DMSO is about 0.4% (4 mg/mL). Substantial heating is again needed to achieve solution with methanol or DMSO, but once the cyclopamine is dissolved it will remain in solution if kept at room temperature. Storage in either solvent at the recommended -20 ºC will also cause crystallization, as noted above for ethanol, and reheating is necessary after thawing in order to achieve full dissolution. Methanol is less desirable than the other two solvents because it is the most volatile; merely sta
C-8700 Cyclopamine, Free Base, 99%[11-Deoxyjervine] M.W. 411.62 C27H41NO2 [4449-51-8] M.I. 14: 10201 SPECIAL HAZARD: TERATOGEN. Gloves and mask should be worn when using this compound. Care must be taken to prevent contact through all routes of exposure. Women of childbearing age should be extremely careful in handling cyclopamine! Storage: Store at or below -20 ºC. Solubility: See below. Disposal: A •Cyclopamine blocks activation of the Hedgehog response pathway associated with mutations that either activate the proto-oncogene Smoothened (Smo) or inactivate the tumour suppressor Patched (Ptch) both of which encode multipass transmembrane proteins. Furthermore, it has been demonstrated that the inhibitory effect of cyclopamine results from its direct binding to the Smo heptahelical protein bundle. Taipale, J., et al. “Effects of oncogenic mutations in Smoothened and Patched can be reversed by cyclopamine.” Nature 406: 944-945 (2000). Chen, J.K., et al. “Inhibition of Hedgehog signaling by direct binding of cyclopamine to Smoothened.” Genes & Dev. 16: 2743-2748 (2002). •Induces chick embryo malformations associated with the interruption of Sonic hedgehog (Shh)-mediated dorsoventral patterning. Incardona, J.P., et al. “The teratogenic Veratrum alkaloid cyclopamine inhibits sonic hedgehog signal transduction.” Development. 125: 3553-3562 (1998). •Displays anti-tumor properties. It has been shown to induce apoptosis in both colorectal adenoma- and carcinoma-derived cell lines and in skin basal cell carcinomas. Qualtrough, D., et al. “Hedgehog signalling in colorectal tumour cells: induction of apoptosis with cyclopamine treatment.” Int J Cancer. 110: 831-837 (2004). Tabs, S., and Avci, O. “Induction of the differentiation and apoptosis of tumor cells in vivo with efficiency and selectivity.” Eur J Dermatol. 14: 96-102 (2004). •Pregnant cattle, goats, or sheep who graze on the corn lily plant, Veratrum californicum, at the 14th day of gestation (for sheep) can give birth to deformed offspring with a characteristic single eye in the middle of the forehead. [For example, view www.cybergoat.com/cyclopia/cyclopia.htm.] This type of birth defect recalls one of the most unforgettable images in all of mythology, namely Homer’s Cyclops (for which cyclopamine was named). If plants containing cyclopamine or a similar compound existed in the Mediterranean area in ancient times, it would then seem likely that the Cyclops was not merely a product of Homer’s imagination but rather was based on known, naturally-occurring birth defects in animals. Alternatively, this type of birth defect might arise from spontaneous mutation, as may have been the case for a kitten recently reported: www.samtsai.com/p131. •CYCLOPAMINE SOLUBILITY: Ethanol is the best cyclopamine solvent for biological use. A 2% solution (20 mg/mL) can be prepared by warming the cyclopamine in ethanol to 50-60 ºC; boiling is neither necessary nor recommended. Upon cooling, the cyclopamine remains in solution at room temperature, even if left for several days. However, storage at -20 ºC is recommended, and at this temperature the cyclopamine will crystallize out of solution. Upon thawing, the mixture will need to be heated again to redissolve the cyclopamine. Methanol and DMSO can also be used as cyclopamine solvents, but solubility in methanol is only about 0.7% (7 mg/mL) at best, and solubility in DMSO is about 0.4% (4 mg/mL). Substantial heating is again needed to achieve solution with methanol or DMSO, but once the cyclopamine is dissolved it will remain in solution if kept at room temperature. Storage in either solvent at the recommended -20 ºC will also cause crystallization, as noted above for ethanol, and reheating is necessary after thawing in order to achieve full dissolution. Methanol is less desirable than the other two solvents because it is the most volatile; merely sta
C-8700 Cyclopamine, Free Base, 99%[11-Deoxyjervine] M.W. 411.62 C27H41NO2 [4449-51-8] M.I. 14: 10201 SPECIAL HAZARD: TERATOGEN. Gloves and mask should be worn when using this compound. Care must be taken to prevent contact through all routes of exposure. Women of childbearing age should be extremely careful in handling cyclopamine! Storage: Store at or below -20 ºC. Solubility: See below. Disposal: A •Cyclopamine blocks activation of the Hedgehog response pathway associated with mutations that either activate the proto-oncogene Smoothened (Smo) or inactivate the tumour suppressor Patched (Ptch) both of which encode multipass transmembrane proteins. Furthermore, it has been demonstrated that the inhibitory effect of cyclopamine results from its direct binding to the Smo heptahelical protein bundle. Taipale, J., et al. “Effects of oncogenic mutations in Smoothened and Patched can be reversed by cyclopamine.” Nature 406: 944-945 (2000). Chen, J.K., et al. “Inhibition of Hedgehog signaling by direct binding of cyclopamine to Smoothened.” Genes & Dev. 16: 2743-2748 (2002). •Induces chick embryo malformations associated with the interruption of Sonic hedgehog (Shh)-mediated dorsoventral patterning. Incardona, J.P., et al. “The teratogenic Veratrum alkaloid cyclopamine inhibits sonic hedgehog signal transduction.” Development. 125: 3553-3562 (1998). •Displays anti-tumor properties. It has been shown to induce apoptosis in both colorectal adenoma- and carcinoma-derived cell lines and in skin basal cell carcinomas. Qualtrough, D., et al. “Hedgehog signalling in colorectal tumour cells: induction of apoptosis with cyclopamine treatment.” Int J Cancer. 110: 831-837 (2004). Tabs, S., and Avci, O. “Induction of the differentiation and apoptosis of tumor cells in vivo with efficiency and selectivity.” Eur J Dermatol. 14: 96-102 (2004). •Pregnant cattle, goats, or sheep who graze on the corn lily plant, Veratrum californicum, at the 14th day of gestation (for sheep) can give birth to deformed offspring with a characteristic single eye in the middle of the forehead. [For example, view www.cybergoat.com/cyclopia/cyclopia.htm.] This type of birth defect recalls one of the most unforgettable images in all of mythology, namely Homer’s Cyclops (for which cyclopamine was named). If plants containing cyclopamine or a similar compound existed in the Mediterranean area in ancient times, it would then seem likely that the Cyclops was not merely a product of Homer’s imagination but rather was based on known, naturally-occurring birth defects in animals. Alternatively, this type of birth defect might arise from spontaneous mutation, as may have been the case for a kitten recently reported: www.samtsai.com/p131. •CYCLOPAMINE SOLUBILITY: Ethanol is the best cyclopamine solvent for biological use. A 2% solution (20 mg/mL) can be prepared by warming the cyclopamine in ethanol to 50-60 ºC; boiling is neither necessary nor recommended. Upon cooling, the cyclopamine remains in solution at room temperature, even if left for several days. However, storage at -20 ºC is recommended, and at this temperature the cyclopamine will crystallize out of solution. Upon thawing, the mixture will need to be heated again to redissolve the cyclopamine. Methanol and DMSO can also be used as cyclopamine solvents, but solubility in methanol is only about 0.7% (7 mg/mL) at best, and solubility in DMSO is about 0.4% (4 mg/mL). Substantial heating is again needed to achieve solution with methanol or DMSO, but once the cyclopamine is dissolved it will remain in solution if kept at room temperature. Storage in either solvent at the recommended -20 ºC will also cause crystallization, as noted above for ethanol, and reheating is necessary after thawing in order to achieve full dissolution. Methanol is less desirable than the other two solvents because it is the most volatile; merely sta
C-8700 Cyclopamine, Free Base, 99%[11-Deoxyjervine] M.W. 411.62 C27H41NO2 [4449-51-8] M.I. 14: 10201 SPECIAL HAZARD: TERATOGEN. Gloves and mask should be worn when using this compound. Care must be taken to prevent contact through all routes of exposure. Women of childbearing age should be extremely careful in handling cyclopamine! Storage: Store at or below -20 ºC. Solubility: See below. Disposal: A •Cyclopamine blocks activation of the Hedgehog response pathway associated with mutations that either activate the proto-oncogene Smoothened (Smo) or inactivate the tumour suppressor Patched (Ptch) both of which encode multipass transmembrane proteins. Furthermore, it has been demonstrated that the inhibitory effect of cyclopamine results from its direct binding to the Smo heptahelical protein bundle. Taipale, J., et al. “Effects of oncogenic mutations in Smoothened and Patched can be reversed by cyclopamine.” Nature 406: 944-945 (2000). Chen, J.K., et al. “Inhibition of Hedgehog signaling by direct binding of cyclopamine to Smoothened.” Genes & Dev. 16: 2743-2748 (2002). •Induces chick embryo malformations associated with the interruption of Sonic hedgehog (Shh)-mediated dorsoventral patterning. Incardona, J.P., et al. “The teratogenic Veratrum alkaloid cyclopamine inhibits sonic hedgehog signal transduction.” Development. 125: 3553-3562 (1998). •Displays anti-tumor properties. It has been shown to induce apoptosis in both colorectal adenoma- and carcinoma-derived cell lines and in skin basal cell carcinomas. Qualtrough, D., et al. “Hedgehog signalling in colorectal tumour cells: induction of apoptosis with cyclopamine treatment.” Int J Cancer. 110: 831-837 (2004). Tabs, S., and Avci, O. “Induction of the differentiation and apoptosis of tumor cells in vivo with efficiency and selectivity.” Eur J Dermatol. 14: 96-102 (2004). •Pregnant cattle, goats, or sheep who graze on the corn lily plant, Veratrum californicum, at the 14th day of gestation (for sheep) can give birth to deformed offspring with a characteristic single eye in the middle of the forehead. [For example, view www.cybergoat.com/cyclopia/cyclopia.htm.] This type of birth defect recalls one of the most unforgettable images in all of mythology, namely Homer’s Cyclops (for which cyclopamine was named). If plants containing cyclopamine or a similar compound existed in the Mediterranean area in ancient times, it would then seem likely that the Cyclops was not merely a product of Homer’s imagination but rather was based on known, naturally-occurring birth defects in animals. Alternatively, this type of birth defect might arise from spontaneous mutation, as may have been the case for a kitten recently reported: www.samtsai.com/p131. •CYCLOPAMINE SOLUBILITY: Ethanol is the best cyclopamine solvent for biological use. A 2% solution (20 mg/mL) can be prepared by warming the cyclopamine in ethanol to 50-60 ºC; boiling is neither necessary nor recommended. Upon cooling, the cyclopamine remains in solution at room temperature, even if left for several days. However, storage at -20 ºC is recommended, and at this temperature the cyclopamine will crystallize out of solution. Upon thawing, the mixture will need to be heated again to redissolve the cyclopamine. Methanol and DMSO can also be used as cyclopamine solvents, but solubility in methanol is only about 0.7% (7 mg/mL) at best, and solubility in DMSO is about 0.4% (4 mg/mL). Substantial heating is again needed to achieve solution with methanol or DMSO, but once the cyclopamine is dissolved it will remain in solution if kept at room temperature. Storage in either solvent at the recommended -20 ºC will also cause crystallization, as noted above for ethanol, and reheating is necessary after thawing in order to achieve full dissolution. Methanol is less desirable than the other two solvents because it is the most volatile; merely sta
C-8700 Cyclopamine, Free Base, 99%[11-Deoxyjervine] M.W. 411.62 C27H41NO2 [4449-51-8] M.I. 14: 10201 SPECIAL HAZARD: TERATOGEN. Gloves and mask should be worn when using this compound. Care must be taken to prevent contact through all routes of exposure. Women of childbearing age should be extremely careful in handling cyclopamine! Storage: Store at or below -20 ºC. Solubility: See below. Disposal: A •Cyclopamine blocks activation of the Hedgehog response pathway associated with mutations that either activate the proto-oncogene Smoothened (Smo) or inactivate the tumour suppressor Patched (Ptch) both of which encode multipass transmembrane proteins. Furthermore, it has been demonstrated that the inhibitory effect of cyclopamine results from its direct binding to the Smo heptahelical protein bundle. Taipale, J., et al. “Effects of oncogenic mutations in Smoothened and Patched can be reversed by cyclopamine.” Nature 406: 944-945 (2000). Chen, J.K., et al. “Inhibition of Hedgehog signaling by direct binding of cyclopamine to Smoothened.” Genes & Dev. 16: 2743-2748 (2002). •Induces chick embryo malformations associated with the interruption of Sonic hedgehog (Shh)-mediated dorsoventral patterning. Incardona, J.P., et al. “The teratogenic Veratrum alkaloid cyclopamine inhibits sonic hedgehog signal transduction.” Development. 125: 3553-3562 (1998). •Displays anti-tumor properties. It has been shown to induce apoptosis in both colorectal adenoma- and carcinoma-derived cell lines and in skin basal cell carcinomas. Qualtrough, D., et al. “Hedgehog signalling in colorectal tumour cells: induction of apoptosis with cyclopamine treatment.” Int J Cancer. 110: 831-837 (2004). Tabs, S., and Avci, O. “Induction of the differentiation and apoptosis of tumor cells in vivo with efficiency and selectivity.” Eur J Dermatol. 14: 96-102 (2004). •Pregnant cattle, goats, or sheep who graze on the corn lily plant, Veratrum californicum, at the 14th day of gestation (for sheep) can give birth to deformed offspring with a characteristic single eye in the middle of the forehead. [For example, view www.cybergoat.com/cyclopia/cyclopia.htm.] This type of birth defect recalls one of the most unforgettable images in all of mythology, namely Homer’s Cyclops (for which cyclopamine was named). If plants containing cyclopamine or a similar compound existed in the Mediterranean area in ancient times, it would then seem likely that the Cyclops was not merely a product of Homer’s imagination but rather was based on known, naturally-occurring birth defects in animals. Alternatively, this type of birth defect might arise from spontaneous mutation, as may have been the case for a kitten recently reported: www.samtsai.com/p131. •CYCLOPAMINE SOLUBILITY: Ethanol is the best cyclopamine solvent for biological use. A 2% solution (20 mg/mL) can be prepared by warming the cyclopamine in ethanol to 50-60 ºC; boiling is neither necessary nor recommended. Upon cooling, the cyclopamine remains in solution at room temperature, even if left for several days. However, storage at -20 ºC is recommended, and at this temperature the cyclopamine will crystallize out of solution. Upon thawing, the mixture will need to be heated again to redissolve the cyclopamine. Methanol and DMSO can also be used as cyclopamine solvents, but solubility in methanol is only about 0.7% (7 mg/mL) at best, and solubility in DMSO is about 0.4% (4 mg/mL). Substantial heating is again needed to achieve solution with methanol or DMSO, but once the cyclopamine is dissolved it will remain in solution if kept at room temperature. Storage in either solvent at the recommended -20 ºC will also cause crystallization, as noted above for ethanol, and reheating is necessary after thawing in order to achieve full dissolution. Methanol is less desirable than the other two solvents because it is the most volatile; merely sta
Cyclopamine, V. californicum A cell-permeable steroidal alkaloid and cholesterol mimic that displays both teratogenic and antitumor activities. It disrupts cholesterol bio-synthesis and specifically antagonizes shh (Sonic Hedgehog) signaling pathway through direct interaction with Smo (smoothened), a distant relative of G-protein-coupled receptors. A valuable tool for studying the involvement of shh signaling in the development of various tumors. Tomatidine (Cat. No. 614350) can be used as a negative control. Also available as a 10 mM solution in Ethanol (Cat. No. 239806). 产品信息 Form Off-white solid Primary Target Hh signaling in Shh-light2 assay Primary Target IC50 20 nM Secondary target in P2Ptch-/-cells (IC50 = 50 nM) Molar mass 411.6 Protect from Light 是的 Packaged under inert gas 是的 Cell permeable 是的 Purity ≥97% by HPLC Solubility DMSO, EtOH, or DMF Chemical formula C27H41NO2 RTECS GY0750000 CAS number 4449-51-8 商店和运输信息 Storage -20°C Ship Shipped with Blue Ice or with Dry Ice Carcinogenic / Teratogenic
Cyclopamine-KAAD 3-Keto-N-(aminoethyl-aminocaproyl-dihydrocinnamoyl)cyclopamine, KAAD-Cyclopamine, Shh Signaling Antagonist II A potent, cell-permeable analog of Cyclopamine (Cat. No. 239803) that specifically inhibits the Hedgehog (Hh) signaling with similar or lower toxicity (IC50 = 20 nM in Shh-LIGHT2 assay; 50 nM in p2Ptch-/-cells; 500 nM in SmoA1-LIGHT cells). Binds to SmoA1 and promotes its exit from the endoplasmic reticulum. Suppresses both the ShhNp-induced pathway activity and SmoA1-induced reporter activity. Shown to sensitize human glioma cells to TRAIL-induced apoptosis. Also available as a 1 mM solution in DMSO (Cat. No. 239807). 产品信息 Form Pale yellow solid Primary Target Hh signaling in Shh-light2 assay Primary Target IC50 20 nM Secondary target in P2Ptch-/-cells (IC50 = 50 nM) Molar mass 698.0 Protect from Light 是的 Packaged under inert gas 是的 Cell permeable 是的 Purity ≥70% by HPLC (sum of two isomers) Solubility DMSO, EtOH, or MeOH Chemical formula C44H63N3O4 商店和运输信息 Storage -20°C Ship Shipped with Blue Ice or with Dry Ice Harmful 安全标示 S Phrase S: 22-36/37 Do not breathe dust. Wear suitable protective clothing and gloves. R Phrase R: 20/21/22 Harmful by inhalation, in contact with skin and if swallowed.
InSolution™ Cyclopamine, V. californicum A cell-permeable steroidal alkaloid and cholesterol mimic that displays both teratogenic and antitumor activities. It disrupts cholesterol bio-synthesis and specifically antagonizes shh (Sonic Hedgehog) signaling pathway through direct interaction with Smo (smoothened), a distant relative of G-protein-coupled receptors. A valuable tool for studying the involvement of shh signaling in the development of various tumors. Tomatidine (Cat. No. 614350) can be used as a negative control. The solid form of this compound (Cat. No. 239803) is also available. 产品信息 Form Liquid Formulation Supplied as a 10 mM (5 mg/1.22 mL) solution of Cyclopamine, V. californicum (Cat. No. 239803) in EtOH. Molar mass 411.6 Protect from Light 是的 Hygrocopic 是的 Avoid freeze/thaw 是的 Packaged under inert gas 是的 Purity ≥97% by NMR Chemical formula C27H41NO2 商店和运输信息 Hazardous Materials Attention: Due to the nature of the Hazardous Materials in this shipment, additional shipping chargesmay be applied to your order. Certain sizes may be exempt from the additional hazardous materials shipping charges. Please contact your local sales office for more information regarding these charges. Storage ≤ -70°C Ship Dry Ice Only Flammable 安全标示 S Phrase S: 16-7 Keep away from sources of ignition – No Smoking. Keep container tightly closed. R Phrase R: 10 Flammable.
Cyclopamine Description A steroidal alkaloid isolated from the desert plant Veratrum californicum with both teratogenic and antitumor activities. It inhibits hedgehog/smoothened signaling. CYCLOPAMINE SOLUBILITY: Ethanol is the best cyclopamine solvent for biological use. A 2% solution (20 mg/mL) can be prepared by warming the cyclopamine in ethanol to 50-60 ºC; boiling is neither necessary nor recommended. Upon cooling, the cyclopamine remains in solution at room temperature, even if left for several days. However, storage at -20 ºC is recommended, and at this temperature the cyclopamine will crystallize out of solution. Upon thawing, the mixture will need to be heated again to redissolve the cyclopamine. Methanol and DMSO can also be used as cyclopamine solvents, but solubility in methanol is only about 0.7% (7 mg/mL) at best, and solubility in DMSO is about 0.4% (4 mg/mL). Substantial heating is again needed to achieve solution with methanol or DMSO, but once the cyclopamine is dissolved it will remain in solution if kept at room temperature. Storage in either solvent at the recommended -20 ºC will also cause crystallization, as noted above for ethanol, and reheating is necessary after thawing in order to achieve full dissolution. Specifications Cas No. 4449-51-8 Product ID C9710 Product Name Cyclopamine Chemical Name Spiro(9H-benzo(a)fluorene-9,2′(3’H)-furo(3,2-b)pyridin)-3- ol, 1,2,3,3’a,4,4′,5′,6,6′,6a,6b,7,7′,7’a,8,11,11a,11b- octadecahydro-3′,6′,10,11b-tetramethyl-, (2’R,3S,3’R,3’aS,6’S,6aS,6bS,7’aR,11aS,11bR)- Synonym 11-Deoxyjervine; 11-Deoxojervine; HSDB 3505. Formula C27H41NO2 Formula Wt. 411.62 Purity ≥98% Solubility Soluble in methanol (.7mg/mL). Soluble in DMSO & insoluble in water. Store Temp -20oC References Coventry S, Kapur RP, Siebert JR. Pediatr Devel Pathol. 1:29-41 (1998). Chen JK, Taipale J, Cooper MK et al. Gene Dev. 16:2743-8 (2002). Qualtrough D, Buda A, Gaffield W et al. Int J Cancer. 110:831-7 (2004).
Cyclopamine Description A steroidal alkaloid isolated from the desert plant Veratrum californicum with both teratogenic and antitumor activities. It inhibits hedgehog/smoothened signaling. CYCLOPAMINE SOLUBILITY: Ethanol is the best cyclopamine solvent for biological use. A 2% solution (20 mg/mL) can be prepared by warming the cyclopamine in ethanol to 50-60 ºC; boiling is neither necessary nor recommended. Upon cooling, the cyclopamine remains in solution at room temperature, even if left for several days. However, storage at -20 ºC is recommended, and at this temperature the cyclopamine will crystallize out of solution. Upon thawing, the mixture will need to be heated again to redissolve the cyclopamine. Methanol and DMSO can also be used as cyclopamine solvents, but solubility in methanol is only about 0.7% (7 mg/mL) at best, and solubility in DMSO is about 0.4% (4 mg/mL). Substantial heating is again needed to achieve solution with methanol or DMSO, but once the cyclopamine is dissolved it will remain in solution if kept at room temperature. Storage in either solvent at the recommended -20 ºC will also cause crystallization, as noted above for ethanol, and reheating is necessary after thawing in order to achieve full dissolution. Specifications Cas No. 4449-51-8 Product ID C9710 Product Name Cyclopamine Chemical Name Spiro(9H-benzo(a)fluorene-9,2′(3’H)-furo(3,2-b)pyridin)-3- ol, 1,2,3,3’a,4,4′,5′,6,6′,6a,6b,7,7′,7’a,8,11,11a,11b- octadecahydro-3′,6′,10,11b-tetramethyl-, (2’R,3S,3’R,3’aS,6’S,6aS,6bS,7’aR,11aS,11bR)- Synonym 11-Deoxyjervine; 11-Deoxojervine; HSDB 3505. Formula C27H41NO2 Formula Wt. 411.62 Purity ≥98% Solubility Soluble in methanol (.7mg/mL). Soluble in DMSO & insoluble in water. Store Temp -20oC References Coventry S, Kapur RP, Siebert JR. Pediatr Devel Pathol. 1:29-41 (1998). Chen JK, Taipale J, Cooper MK et al. Gene Dev. 16:2743-8 (2002). Qualtrough D, Buda A, Gaffield W et al. Int J Cancer. 110:831-7 (2004).
Cyclopamine Description A steroidal alkaloid isolated from the desert plant Veratrum californicum with both teratogenic and antitumor activities. It inhibits hedgehog/smoothened signaling. CYCLOPAMINE SOLUBILITY: Ethanol is the best cyclopamine solvent for biological use. A 2% solution (20 mg/mL) can be prepared by warming the cyclopamine in ethanol to 50-60 ºC; boiling is neither necessary nor recommended. Upon cooling, the cyclopamine remains in solution at room temperature, even if left for several days. However, storage at -20 ºC is recommended, and at this temperature the cyclopamine will crystallize out of solution. Upon thawing, the mixture will need to be heated again to redissolve the cyclopamine. Methanol and DMSO can also be used as cyclopamine solvents, but solubility in methanol is only about 0.7% (7 mg/mL) at best, and solubility in DMSO is about 0.4% (4 mg/mL). Substantial heating is again needed to achieve solution with methanol or DMSO, but once the cyclopamine is dissolved it will remain in solution if kept at room temperature. Storage in either solvent at the recommended -20 ºC will also cause crystallization, as noted above for ethanol, and reheating is necessary after thawing in order to achieve full dissolution. Specifications Cas No. 4449-51-8 Product ID C9710 Product Name Cyclopamine Chemical Name Spiro(9H-benzo(a)fluorene-9,2′(3’H)-furo(3,2-b)pyridin)-3- ol, 1,2,3,3’a,4,4′,5′,6,6′,6a,6b,7,7′,7’a,8,11,11a,11b- octadecahydro-3′,6′,10,11b-tetramethyl-, (2’R,3S,3’R,3’aS,6’S,6aS,6bS,7’aR,11aS,11bR)- Synonym 11-Deoxyjervine; 11-Deoxojervine; HSDB 3505. Formula C27H41NO2 Formula Wt. 411.62 Purity ≥98% Solubility Soluble in methanol (.7mg/mL). Soluble in DMSO & insoluble in water. Store Temp -20oC References Coventry S, Kapur RP, Siebert JR. Pediatr Devel Pathol. 1:29-41 (1998). Chen JK, Taipale J, Cooper MK et al. Gene Dev. 16:2743-8 (2002). Qualtrough D, Buda A, Gaffield W et al. Int J Cancer. 110:831-7 (2004).
