ANTI-CFTR, CLONE M3A7 -200UG

货号： 05-583      产品名称： ANTI-CFTR, CLONE M3A7 -200UG   品牌： Millipore 规格： EA 3周到货 生化实验

Anti-CFTR Antibody, clone M3A7
Close
REFERENCES
CFTR is involved in the fine tuning of intracellular redox status: physiological implications in cystic fibrosis.
Duranton, Christophe, et al. (2012) Am. J. Pathol., 181: 1367-77 (2012)
PDZ domain interaction controls the endocytic recycling of the cystic fibrosis transmembrane conductance regulator.
Swiatecka-Urban, Agnieszka, et al. (2002) J. Biol. Chem., 277: 40099-105 (2002)
C-terminal truncations destabilize the cystic fibrosis transmembrane conductance regulator without impairing its biogenesis. A novel class of mutation.
Haardt, M, et al. (1999) J. Biol. Chem., 274: 21873-7 (1999)
Characterization of polyclonal and monoclonal antibodies to cystic fibrosis transmembrane conductance regulator.
Kartner, N and Riordan, J R (1998) Meth. Enzymol., 292: 629-52 (1998)
View All
Species Reactivity Key Applications Host Format Antibody Type
H  IHC, IP, WB Mouse Purified Monoclonal Antibody
Description:
Anti-CFTR Antibody, clone M3A7
Promotional Text:
Special Shipping Offer on Antibodies
100% Performance Guaranteed
Trade Name:
Upstate (Millipore)
Specificity:
This antibody recognizes CFTR, Mr 170 kDa.
Molecular Weight:
170 kDa
Epitope:
a.a. 1365-1395
Immunogen:
GST-fusion protein corresponding to residues 1197-1480 of human cystic fibrosis transmembrane conductance regulator (CFTR). The epitope maps within amino acids 1365-1395. Clone M3A7.
Clone:
M3A7
Isotype:
IgG1
Background Information:
CFTR (Cystic Fibrosis Transmembrane Regulator) is an integral membrane protein shown to be involved in the transport of chloride ions. It is found on the surface of the epithelial cells lining the lungs and other organs. CFTR defects are the cause of cystic fibrosis. Cystic Fibrosis (CF) is a common lethal genetic disease caused by mutations of the gene coding for the cystic fibrosis transmembrane conductance factor, a cAMP regulated chloride channel. Approximately 70% of all CF cases share the deletion of a phenylalanine at position 508 (delta F508), which results in abnormal chloride transport. Since the CF mutation is lethal, most often by lung and liver disease, it raises the question of why this genetic disease remains as common as it is.
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Species Reactivity:
Human
Species Reactivity Note:
Human. Other species not tested.
Application Notes:
Immunoprecipitation:
This antibody has been reported to immunoprecipitate CFTR. (Kartner, N., 1998.)
Immunohistochemistry:
This antibody has been reported to immunostain CFTR in human pancreatic tissue sections. (Kartner, N., 1998.)
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Control:
T84 cell lysate.
Quality Assurance:
Routinely evaluated by western blot on human T84 colon carcinoma epithelial RIPA cell lysates or from CFTR-transfected BHK2.
Western Blot Analysis:
0.5-2 µg/mL of this lot detected CFTR from 20-50 µg of human T84 colon carcinoma epithelial RIPA cell lysates. 0.5-2 µg/mL of a previous lot detected CFTR from CFTR-transfected BHK (Haardt, M., 1999).
Note: Do not boil the lysate. Instead incubate at 37°C for 30 minutes. CFTR can run as a diffuse protein on SDS-PAGE.
View All »
Purification Method:
Protein G purfied
Presentation:
Purified mouse monoclonal IgG1 in buffer containing 0.1 M Tris-glycine, pH 7.4, 0.15 M NaCl, 0.05% sodium azide and 30% glycerol.
Storage Conditions:
Stable for 1 year at -20°C from date of receipt.
Handling Recommendations:
Upon receipt, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microc -20°C

Anti-CFTR, clone M3A7

货号： 05-583-K      产品名称： Anti-CFTR, clone M3A7   品牌： Millipore 规格： MG 三周到货 生化实验

Anti-CFTR Antibody, clone M3A7
Close
REFERENCES
CFTR is involved in the fine tuning of intracellular redox status: physiological implications in cystic fibrosis.
Duranton, Christophe, et al. (2012) Am. J. Pathol., 181: 1367-77 (2012)
PDZ domain interaction controls the endocytic recycling of the cystic fibrosis transmembrane conductance regulator.
Swiatecka-Urban, Agnieszka, et al. (2002) J. Biol. Chem., 277: 40099-105 (2002)
C-terminal truncations destabilize the cystic fibrosis transmembrane conductance regulator without impairing its biogenesis. A novel class of mutation.
Haardt, M, et al. (1999) J. Biol. Chem., 274: 21873-7 (1999)
Characterization of polyclonal and monoclonal antibodies to cystic fibrosis transmembrane conductance regulator.
Kartner, N and Riordan, J R (1998) Meth. Enzymol., 292: 629-52 (1998)
View All
Species Reactivity Key Applications Host Format Antibody Type
H  IHC, IP, WB Mouse Purified Monoclonal Antibody
Description:
Anti-CFTR Antibody, clone M3A7
Promotional Text:
Special Shipping Offer on Antibodies
100% Performance Guaranteed
Trade Name:
Upstate (Millipore)
Specificity:
This antibody recognizes CFTR, Mr 170 kDa.
Molecular Weight:
170 kDa
Epitope:
a.a. 1365-1395
Immunogen:
GST-fusion protein corresponding to residues 1197-1480 of human cystic fibrosis transmembrane conductance regulator (CFTR). The epitope maps within amino acids 1365-1395. Clone M3A7.
Clone:
M3A7
Isotype:
IgG1
Background Information:
CFTR (Cystic Fibrosis Transmembrane Regulator) is an integral membrane protein shown to be involved in the transport of chloride ions. It is found on the surface of the epithelial cells lining the lungs and other organs. CFTR defects are the cause of cystic fibrosis. Cystic Fibrosis (CF) is a common lethal genetic disease caused by mutations of the gene coding for the cystic fibrosis transmembrane conductance factor, a cAMP regulated chloride channel. Approximately 70% of all CF cases share the deletion of a phenylalanine at position 508 (delta F508), which results in abnormal chloride transport. Since the CF mutation is lethal, most often by lung and liver disease, it raises the question of why this genetic disease remains as common as it is.
View All »
Species Reactivity:
Human
Species Reactivity Note:
Human. Other species not tested.
Application Notes:
Immunoprecipitation:
This antibody has been reported to immunoprecipitate CFTR. (Kartner, N., 1998.)
Immunohistochemistry:
This antibody has been reported to immunostain CFTR in human pancreatic tissue sections. (Kartner, N., 1998.)
View All »
Control:
T84 cell lysate.
Quality Assurance:
Routinely evaluated by western blot on human T84 colon carcinoma epithelial RIPA cell lysates or from CFTR-transfected BHK2.
Western Blot Analysis:
0.5-2 µg/mL of this lot detected CFTR from 20-50 µg of human T84 colon carcinoma epithelial RIPA cell lysates. 0.5-2 µg/mL of a previous lot detected CFTR from CFTR-transfected BHK (Haardt, M., 1999).
Note: Do not boil the lysate. Instead incubate at 37°C for 30 minutes. CFTR can run as a diffuse protein on SDS-PAGE.
View All »
Purification Method:
Protein G purfied
Presentation:
Purified mouse monoclonal IgG1 in buffer containing 0.1 M Tris-glycine, pH 7.4, 0.15 M NaCl, 0.05% sodium azide and 30% glycerol.
Storage Conditions:
Stable for 1 year at -20°C from date of receipt.
Handling Recommendations:
Upon receipt, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microc -20℃