标签归档：cdra

05-1413   Anti-CD45RA, Clone MEM 56 (mouse monoclonal)   品牌 Millipore

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Anti-CD45RA, Clone MEM 56 (mouse monoclonal)

货号： 05-1413      产品名称： Anti-CD45RA, Clone MEM 56 (mouse monoclonal)   品牌： Millipore 规格： EA 3周到货 生化实验

Anti-CD45RA Antibody, Clone MEM 56
Recommended Replacement for: 05-1413 is a recommended replacement for CBL465
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Species Reactivity Key Applications Host Format Antibody Type
H, M, R  FC, IF, IH(P), IP, WB Mouse Purified Monoclonal Antibody
Description:
Anti-CD45RA Antibody, Clone MEM 56
Replaces:
05-1413 is a recommended replacement for CBL465
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Specificity:
This antibody recognizes the 220 kDa molecular weight component of the leucocyte common antigen complex expressed on some CD4+ T lymphocytes, B lymphocytes and CD8+ lymphocytes. The CD45R+/CD4+ lymphocytes functionally exert suppressor/inducer activity in in vitro assay systems.
Antigen distribution:
B-cells (lg+) > 95%
T-cells (E+) 70 + 10%
Peripheral blood lymphocytes 68 + 8%
T-cells (CD4+) 50 + 10%
Monocytes 50 + 20%
Thymocytes < 5%
Granulocytes < 1%
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Molecular Weight:
~220 kDa
Clone:
MEM 56
Isotype:
IgG2b
Background Information:
CD45 is a receptor-tyrosine phosphatase required for antigen-induced signaling in T-cells and B-cells; its substrates are unknown. Interestingly, it associates with several Src-family tyrosine kinases, which are inhibited by tyrosine phosphorylation in their c-termini, suggesting a mechanism for signaling by CD45.
Species Reactivity:
Human
Mouse
Rat
Species Reactivity Note:
Mouse. Expected to react with human and rat based on sequence homology. .
Application Notes:
Immunofluorescence: Identification of CD4+ suppressor/inducer cells, by indirect immunofluorescence staining.
Immunoprecipitation: A previous lot of this antibody detected 220 kDa molecular weight component of the leucocyte common antigen complex in immunoprecipitation.
Flow Cytometry: Purification or depletion of CD4+ suppressor/inducer cells by cell sorting or the panning techniques was performed on a previous lot.
Immunohistochemistry(paraffin): Representative testing from a previous lot.
Identification of CD45R+ lymphocytes in cryostat sections of frozen tissue and in formalin fixed wax embedded tissue.
Optimal Staining of CD45RA Monoclonal Antibody: Tonsil
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Control:
RAW264 Cell Lysate
Quality Assurance:
Routinely evaluated by Western Blot on RAW 264 lysates.
Western Blot Analysis: 1:500 dilution of this lot detected CD45RA on 10 μg of RAW 264
Purification Method:
Purified
Presentation:
Purified mouse monoclonal IgG2b in phosphate buffered saline containing 10 mM sodium azide and 1 mg/mL bovine serum albumin.
We recommend that each laboratory determine an optimum working titre for use in its particular application.
Storage Conditions:
For use within 1 month of date of purchase store at 2-8°C, for long term storage aliquot antibody into small volumes and store at -20°C for up to 1 year from date of receipt.
Handling Recommendations: Upon first thaw, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
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UniProt Number:
P08575
Entrez Gene Number:
NM_002838.3
Gene Symbol:
PTPRC
L-CA
LCA
CD45
LY5
T200
Glycoprotein
B220
GP180
EC 3.1.3.48
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Alternate Names:
CD45 antigen
Leukocyte common anti 2°C – 8°C