标签归档：brk

7708-5   BRK, Active   品牌 Biovision

发表于由

BRK, Active

货号： 7708-5      产品名称： BRK, Active   品牌： Biovision 规格： 5 μg 3周到货 生化实验

Active BRK
An intracellular signal transducer in epithelial tissues.
Product Overview
Alternate Names / Synonyms: Active BRK, BRK, human BRK, active kinase, active kinases, recombinant BRK, recombinant BRK, kinase activity assay, protein kinase
Gene Symbol: PTK6
Accession #: NM_005975.
Gene ID: 5753
Source: Baculovirus (Sf9 insect cells)
Appearance: Liquid
Physical Form Description: Recombinant protein in storage buffer (50 mM Tris-HCl, pH 7.5, 150 mM NaCl, 0.25 mM DTT, 0.1 mM EGTA, 0.1 mM EDTA, 0.1 mM PMSF, 25% glycerol).
Molecular Weight: ~80.0 kDa
Purity by SDS-PAGE: ≥95%
Purity by HPLC: N/A
Endotoxin Level: N/A
Biological Activity: 133 nmol phosphate incorporated into Poly (Glu-Tyr) per minute per mg protein at 30°C for 15 minutes using a final concentration of 50 µM ATP and total of 0.83 μCi/µl P-32.
Reconstitution Instructions: N/A
Storage Temp.: -80°C
Shipping: gel pack
Background Information: BRK is another member of the non-receptor tyrosine kinases (PTKs) that contains an amino terminal SH3 and SH2 domains as well as the catalytic domain (1). Although BRK shows strongest sequence similarity to members of the Src family, there are several key structural and regulatory differences that place it on its own amongst non-receptor PTKs. The genomic structure of BRK consists of 8 exons, whose boundaries are distinct from other non-receptor PTK family members (2). Alternate splicing of the primary BRK transcript generates a distinct mRNA which encodes a truncated protein consisting of an SH3 domain and a novel C-terminal proline rich sequence. Brk transcript is expressed in the human breast tumor cell line and expression of a tumor derived Brk cDNA in mouse embryonic fibroblasts and human mammary epithelial cells supports anchorage independent growth, and in the latter potentiates the mitogenic response to epidermal growth factor. Brk expression is low or undetectable in normal mammary tissue and benign lesions. However, approximately two-thirds of breast tumors express appreciable levels and 27% of tumors over express BRK by fivefold or more (up to 43x). This expression pattern is mirrored in comparison of cell lines derived either from normal mammary epithelial cells or from carcinomas (3).
Handling: Centrifuge the vial prior to opening.
Usage: For Research Use Only! Not to be used in humans.

-80°C

09-143   BRK,RBX,50ug   品牌 Millipore

发表于由

BRK,RBX,50ug

货号： 09-143      产品名称： BRK,RBX,50ug   品牌： Millipore 规格： EA 三周到货 生化实验

Anti-Brk Antibody
Close
REFERENCES
Growth response of Escherichia coli ATCC 35218 adapted to several concentrations of sodium benzoate and potassium sorbate.
Santiesteban-López NA, Rosales M, Palou E, López-Malo A (2009) J Food Prot.72:2301-7.
The intracellular tyrosine kinase Brk sensitizes non-transformed cells to inducers of apoptosis
Haegebarth, Andrea, et al (2005) Cell Cycle, 4:1239-46 (2005)
Regulation of the nonreceptor tyrosine kinase Brk by autophosphorylation and by autoinhibition
Qiu, Haoqun and Miller, W Todd (2002) J Biol Chem, 277:34634-41 (2002)
Interaction between Brk kinase and insulin receptor substrate-4
Qiu, Haoqun, et al (2005) Oncogene, 24:5656-64 (2005)
View All
Species Reactivity Key Applications Host Format Antibody Type
H  WB Rabbit Affinity Purified Polyclonal Antibody
Description:
Anti-Brk Antibody
Promotional Text:
Special Shipping Offer on Antibodies
100% Performance Guaranteed
Trade Name:
Upstate (Millipore)
Specificity:
Recognizes Brk (breast tumor kinase, PTK6) at approximately 52 kDa. It can also recognize the 73 kDa ELF-related protein that shares 70% homology with the immunizing peptide.
Molecular Weight:
52 kDa
Immunogen:
KLH conjugated synthetic peptide corresponding to the N-terminal region of human Brk.
Isotype:
IgG
Species Reactivity:
Human
Quality Assurance:
Routinely evaluated by immunoblot.
Purification Method:
Immunoaffinity purified
Presentation:
50 µg of affinity purified rabbit polyclonal IgG in 0.02 M PBS, 250 mM sodium chloride containing 0.1% sodium azide.
Storage Conditions:
Store for 1 year at 2-8°C from date of shipment.
UniProt Number:
Q13882
Entrez Gene Number:
NM_005975.2
Gene Symbol:
PTK6
BRK
FLJ42088
Alternate Names:
PTK6, protein tyrosine kinase 6, breast tumor kinase, protein-tyrosine kinase BRK,
Usage Statement:
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
View All »
Key Applications:
Western Blotting
Entrez Gene Summary:
The protein encoded by this gene is a cytoplasmic nonreceptor protein kinase which may function as an intracellular signal transducer in epithelial tissues. Overexpression of this gene in mammary epithelial cells leads to sensitization of the cells to epidermal growth factor and results in a partially transformed phenotype. Expression of this gene has been detected at low levels in some breast tumors but not in normal breast tissue. The encoded protein has been shown to undergo autophosphorylation.
View All »
UniProt Summary:
FUNCTION: SwissProt: Q13882 # Phosphorylates KHDRBS1, KHDRBS2, KHDRBS3 and STAP2/BKS. May function as an intracellular signal transducer in epithelial tissues. Overexpression in mammary cells leads to mitogenically sensitization to EGF, and results in a partially transformed phenotype. Its presence in the nucleus appears to be linked to suppression of tumor progression.
SIZE: 451 amino acids; 51834 Da
SUBUNIT: Interacts with GAP-A.p65 (By similarity). Interacts with KHDRBS1.
SUBCELLULAR LOCATION: Cytoplasm. Nucleus. Note=Colocalizes with KHDRBS1, KHDRBS2 or KHDRBS3, within the nucleus. In secretory epithelial cells from prostate adenocarcinoma, nuclear localization is higher in low-grade and l 2-8℃

09-143-K   BRK, RBX, -MG   品牌 Millipore

发表于由

BRK, RBX, -MG

货号： 09-143-K      产品名称： BRK, RBX, -MG   品牌： Millipore 规格： MG 三周到货 生化实验

Anti-Brk Antibody
Close
REFERENCES
Growth response of Escherichia coli ATCC 35218 adapted to several concentrations of sodium benzoate and potassium sorbate.
Santiesteban-López NA, Rosales M, Palou E, López-Malo A (2009) J Food Prot.72:2301-7.
The intracellular tyrosine kinase Brk sensitizes non-transformed cells to inducers of apoptosis
Haegebarth, Andrea, et al (2005) Cell Cycle, 4:1239-46 (2005)
Regulation of the nonreceptor tyrosine kinase Brk by autophosphorylation and by autoinhibition
Qiu, Haoqun and Miller, W Todd (2002) J Biol Chem, 277:34634-41 (2002)
Interaction between Brk kinase and insulin receptor substrate-4
Qiu, Haoqun, et al (2005) Oncogene, 24:5656-64 (2005)
View All
Species Reactivity Key Applications Host Format Antibody Type
H  WB Rabbit Affinity Purified Polyclonal Antibody
Description:
Anti-Brk Antibody
Promotional Text:
Special Shipping Offer on Antibodies
100% Performance Guaranteed
Trade Name:
Upstate (Millipore)
Specificity:
Recognizes Brk (breast tumor kinase, PTK6) at approximately 52 kDa. It can also recognize the 73 kDa ELF-related protein that shares 70% homology with the immunizing peptide.
Molecular Weight:
52 kDa
Immunogen:
KLH conjugated synthetic peptide corresponding to the N-terminal region of human Brk.
Isotype:
IgG
Species Reactivity:
Human
Quality Assurance:
Routinely evaluated by immunoblot.
Purification Method:
Immunoaffinity purified
Presentation:
50 µg of affinity purified rabbit polyclonal IgG in 0.02 M PBS, 250 mM sodium chloride containing 0.1% sodium azide.
Storage Conditions:
Store for 1 year at 2-8°C from date of shipment.
UniProt Number:
Q13882
Entrez Gene Number:
NM_005975.2
Gene Symbol:
PTK6
BRK
FLJ42088
Alternate Names:
PTK6, protein tyrosine kinase 6, breast tumor kinase, protein-tyrosine kinase BRK,
Usage Statement:
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
View All »
Key Applications:
Western Blotting
Entrez Gene Summary:
The protein encoded by this gene is a cytoplasmic nonreceptor protein kinase which may function as an intracellular signal transducer in epithelial tissues. Overexpression of this gene in mammary epithelial cells leads to sensitization of the cells to epidermal growth factor and results in a partially transformed phenotype. Expression of this gene has been detected at low levels in some breast tumors but not in normal breast tissue. The encoded protein has been shown to undergo autophosphorylation.
View All »
UniProt Summary:
FUNCTION: SwissProt: Q13882 # Phosphorylates KHDRBS1, KHDRBS2, KHDRBS3 and STAP2/BKS. May function as an intracellular signal transducer in epithelial tissues. Overexpression in mammary cells leads to mitogenically sensitization to EGF, and results in a partially transformed phenotype. Its presence in the nucleus appears to be linked to suppression of tumor progression.
SIZE: 451 amino acids; 51834 Da
SUBUNIT: Interacts with GAP-A.p65 (By similarity). Interacts with KHDRBS1.
SUBCELLULAR LOCATION: Cytoplasm. Nucleus. Note=Colocalizes with KHDRBS1, KHDRBS2 or KHDRBS3, within the nucleus. In secretory epithelial cells from prostate adenocarcinoma, nuclear localization is higher in low-grade and l 2-8℃

09-144   p-BRK(y342), RBX-100UG   品牌 Millipore

发表于由

p-BRK(y342), RBX-100UG

货号： 09-144      产品名称： p-BRK(y342), RBX-100UG   品牌： Millipore 规格： EA 三周到货 生化实验

Anti-phospho-Brk (Tyr342) Antibody
Close
REFERENCES
Identification of STAT3 as a specific substrate of breast tumor kinase
Liu, L, et al (2006) Oncogene, 25:4904-12 (2006)
Interaction between Brk kinase and insulin receptor substrate-4
Qiu, Haoqun, et al (2005) Oncogene, 24:5656-64 (2005)
Species Reactivity Key Applications Host Format Antibody Type
H  WB Rabbit Affinity Purified Polyclonal Antibody
Description:
Anti-phospho-Brk (Tyr342) Antibody
Promotional Text:
Special Shipping Offer on Antibodies
100% Performance Guaranteed
Trade Name:
Upstate (Millipore)
Specificity:
Recognizes human phospho-Brk at tyrosine 342.
Molecular Weight:
~50 kDa
Immunogen:
KLH conjugated synthetic peptide corresponding to amino acids encompassing Tyr342 of human Brk.
Modifications:
Phosphorylation
Isotype:
IgG
Species Reactivity:
Human
Quality Assurance:
Routinely evaluated by immunoblot.
Purification Method:
Immunoaffinity purified
Presentation:
Purified rabbit polyclonal in buffer containing 0.1M Tris-Glycine (pH7.4, 150 mM NaCl) with 0.05% sodium azide.
Storage Conditions:
Stable for 1 year at 2-8°C from date of receipt.
UniProt Number:
Q13882
Entrez Gene Number:
NM_005975.2
Gene Symbol:
PTK6
BRK
FLJ42088
Alternate Names:
PTK6, protein tyrosine kinase 6, breast tumor kinase, protein-tyrosine kinase BRK
Usage Statement:
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
View All »
Key Applications:
Western Blotting
Entrez Gene Summary:
The protein encoded by this gene is a cytoplasmic nonreceptor protein kinase which may function as an intracellular signal transducer in epithelial tissues. Overexpression of this gene in mammary epithelial cells leads to sensitization of the cells to epidermal growth factor and results in a partially transformed phenotype. Expression of this gene has been detected at low levels in some breast tumors but not in normal breast tissue. The encoded protein has been shown to undergo autophosphorylation.
View All »
UniProt Summary:
FUNCTION: SwissProt: Q13882 # Phosphorylates KHDRBS1, KHDRBS2, KHDRBS3 and STAP2/BKS. May function as an intracellular signal transducer in epithelial tissues. Overexpression in mammary cells leads to mitogenically sensitization to EGF, and results in a partially transformed phenotype. Its presence in the nucleus appears to be linked to suppression of tumor progression.
SIZE: 451 amino acids; 51834 Da
SUBUNIT: Interacts with GAP-A.p65 (By similarity). Interacts with KHDRBS1.
SUBCELLULAR LOCATION: Cytoplasm. Nucleus. Note=Colocalizes with KHDRBS1, KHDRBS2 or KHDRBS3, within the nucleus. In secretory epithelial cells from prostate adenocarcinoma, nuclear localization is higher in low-grade and lower in high-grade regions of the tumors.
TISSUE SPECIFICITY: Epithelia-specific. Very high level in colon and high levels in small intestine and prostate, and low levels in some fetal tissues. Expressed at low level in some breast tumors, but not in normal breast. Also found in melanocytes. Not expressed in heart, brain, placenta, lung, liver, skeletal muscle, kidney and pancreas.
PTM: Autophosphorylated. The phosphorylation of Tyr-447 may lead to the autoinhibition of the enzyme.
SIMILARITY: Sw 2-8℃