标签： appl

Anti-APPL1

货号： ABS314      产品名称： Anti-APPL1   品牌： Millipore 规格： EA 三周到货 生化实验

Anti-APPL1
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REFERENCES
APPL1 potentiates insulin-mediated inhibition of hepatic glucose production and alleviates diabetes via Akt activation in mice.
Cheng, Kenneth K Y, et al. (2009) Cell Metab., 9: 417-27 (2009)
APPL1 associates with TrkA and GIPC1 and is required for nerve growth factor-mediated signal transduction.
Lin, Dan C, et al. (2006) Mol. Cell. Biol., 26: 8928-41 (2006)
Species Reactivity Key Applications Host Format Antibody Type
H, M, R  WB, IP, ICC, IHC Rabbit Affinity Purified Polyclonal Antibody
Description:
Anti-APPL1 Antibody
Molecular Weight:
~84 kDa observed. Uncharacterized band(s) may be observed in some cell lysates.
Immunogen:
Recombinant protein corresponding to human APPL1.
Background Information:
Adapter protein containing PH domain, PTB domain and leucine zipper motif 1 (APPL1) is also called DCC-interacting protein 13-alpha (Dip13-alpha). APPL1 is necessary for cell proliferation in response to extracellular signals and links Rab5 to nuclear signal transduction. APPL1 is expressed at high levels in heart, ovary, pancreas, and skeletal muscle tissues.
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Species Reactivity:
Human
Mouse
Rat
Application Notes:
Western Blotting Analysis: 0.5 µg/mL of this antibody detected APPL1 in 10 µg of L6 and NIH/3T3 cell lysate.
Immunoprecipitation Analysis: A representative lot from an independent laboratory immunoprecipitated APPL1 in HUVEC cell lysate (Lin, D. C., et al. (2006). Mol Cell Biol. 26(23):8928-8941.).
Immunohistochemistry Analysis: A representative lot from an independent laboratory detected APPL1 in liver tissues from mice fed with either a standard diet, high fat diet, or a high fat diet with exercise (Marinho, R., et al. (2012). J Cell Physiol. 227(7):2917-2926.).
Immunocytochemistry Analysis: A representative lot from an independent laboratory detected APPL1 in pancreatic islets and impaired GSIS cells of dietary and obese mice (Cheng, K. K., et al. (2009). Cell Metab. 9(5):417-427.).
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Quality Assurance:
Evaluated by Western Blotting in A431 cell lysate.
Western Blotting Analysis: 0.5 µg/mL of this antibody detected APPL1 in 10 µg of A431 cell lysate.
Purification Method:
Affinity Purified
Presentation:
Purified rabbit polyclonal in buffer containing PBS with 0.05% sodium azide.
Storage Conditions:
Stable for 1 year at 2-8°C from date of receipt.
UniProt Number:
Q9UKG1
Entrez Gene Number:
NP_036228
Gene Symbol:
APPL1
APPL
DIP13A
Alternate Names:
DCC-interacting protein 13-alpha
Dip13-alpha
Adapter protein containing PH domain, PTB domain and leucine zipper motif 1
Usage Statement:
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Key Applications:
Western Blotting
Immunoprecipitation
Immunocytochemistry
Immunohistochemistry
Product Name:
Anti-APPL1
Concentration:
Please refer to the Certificate of Analysis for the lot-specific concentration.
Antibody Type:
Polyclonal Antibody
Qty/Pk:
100 μg
Format:
Affinity Purified
Host:
Rabbit
Applications:
Detect Adapter protein containing PH domain, PTB domain & leucine zipper motif 1 using this rabbit polyclonal anti 2-8℃