标签归档：adiponectin

EZHADP-61BK   Human Adiponectin ELISA Kit (BULK PACK)   品牌 Millipore

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Human Adiponectin ELISA Kit (BULK PACK)

货号： EZHADP-61BK      产品名称： Human Adiponectin ELISA Kit (BULK PACK)   品牌： Millipore 规格： EA 三周到货 生化实验

Human Adiponectin ELISA
Recommended Replacement for: This product replaces CYT350, CHEMICON® Human Adiponectin ELISA Kit
Description:
Human Adiponectin ELISA
Qty/Pk:
96-well strip plate
Background Information:
Adiponectin is identified as a 30 kDa adipokine that is secreted from the adipose tissue into circulating blood and influences systemic metabolism.
Applications:
This Human Adiponectin ELISA is used to measure & quantify Adiponectin levels in Metabolism & Endocrine research.
Key Applications:
ELISA
Application Notes:
Room Temperature, 3 hour assay using 20 µL sample size (serum/plasma: 1:500 dilution)
Species Reactivity:
Human
Usage Statement:
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Entrez Gene Summary:
This gene is expressed in adipose tissue exclusively. It encodes a protein with similarity to collagens X and VIII and complement factor C1q. The encoded protein circulates in the plasma and is involved with metabolic and hormonal processes.
UniProt Summary:
FUNCTION: SwissProt: Q15848 # Important adipokine involved in the control of fat metabolism and insulin sensitivity, with direct anti-diabetic, anti-atherogenic and anti-inflammatory activities. Stimulates AMPK phosphorylation and activation in the liver and the skeletal muscle, enhancing glucose utilization and fatty-acid combustion. Antagonizes TNF-alpha by negatively regulating its expression in various tissues such as liver and macrophages, and also by counteracting its effects. Inhibits endothelial NF-kappa-B signaling through a cAMP-dependent pathway. May play a role in cell growth, angiogenesis and tissue remodeling by binding and sequestering various growth factors with distinct binding affinities, depending on the type of complex, LMW, MMW or HMW.
SIZE: 244 amino acids; 26414 Da
SUBUNIT: Homomultimer. Forms trimers, hexamers and 12- to 18-mers. The trimers (low molecular weight complexes / LMW) are assembled via non-covalent interactions of the collagen-like domains in a triple helix and hydrophobic interactions within the globular C1q domain. Several trimers can associate to form disulfide-linked hexamers (middle molecular weight complexes / MMW) and larger complexes (higher molecular weight / HMW). The HMW-complex assembly may rely aditionnally on lysine hydroxylation and glycosylation. LMW, MMW and HMW complexes bind to HBEGF, MMW and HMW complexes bind to PDGFB, and HMW complex binds to FGF2.
SUBCELLULAR LOCATION: Secreted.
TISSUE SPECIFICITY: Synthesized exclusively by adipocytes and secreted into plasma.DOMAIN:SwissProt: Q15848 The C1q domain is commonly called the globular domain.
PTM: Hydroxylated Lys-33 was not identified in PubMed:16497731, probably due to poor representation of the N-terminal peptide in mass fingerprinting. & HMW complexes are more extensively glycosylated than smaller oligomers. Hydroxylation and glycosylation of the lysine residues within the collagene-like domain of adiponectin seem to be critically involved in regulating the formation and/or secretion of HMW complexes and consequently contribute to the insulin- sensitizing activity of adiponectin in hepatocytes (By similarity). & O-linked glycans consist of Glc-Gal disaccharides bound to the oxygen atom of post-translationally added hydroxyl groups. & Not N-glycosylated.
DISEASE: SwissProt: Q15848 # Defects in ADIPOQ are the cause of adiponectin deficiency [MIM:605441]. The result is a very low RT

EZMADP-60BK   Mouse Adiponectin ELISA Kit (BULKPAK)   品牌 Millipore

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Mouse Adiponectin ELISA Kit (BULKPAK)

货号： EZMADP-60BK      产品名称： Mouse Adiponectin ELISA Kit (BULKPAK)   品牌： Millipore 规格： EA 三周到货 生化实验

Mouse Adiponectin ELISA
Recommended Replacement for: This product replaces CYT286, CHEMICON® Mouse Adiponectin ELISA Kit
Description:
Mouse Adiponectin ELISA
Qty/Pk:
96-well plate
Background Information:
Adiponectin is identified as a 30 kDa adipokine that is secreted from the adipose tissue into circulating blood and influences systemic metabolism.
Applications:
This Mouse Adiponectin ELISA is used to measure & quantify Adiponectin levels in Metabolism & Endocrine research.
Key Applications:
ELISA
Application Notes:
Room temperature, 3 hour assay 20 μL sample size (serum/plasma: 1:1000 dilution)
Species Reactivity:
Mouse
Usage Statement:
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
View All »
Entrez Gene Summary:
This gene is expressed in adipose tissue exclusively. It encodes a protein with similarity to collagens X and VIII and complement factor C1q. The encoded protein circulates in the plasma and is involved with metabolic and hormonal processes.
UniProt Summary:
FUNCTION: SwissProt: Q15848 # Important adipokine involved in the control of fat metabolism and insulin sensitivity, with direct anti-diabetic, anti-atherogenic and anti-inflammatory activities. Stimulates AMPK phosphorylation and activation in the liver and the skeletal muscle, enhancing glucose utilization and fatty-acid combustion. Antagonizes TNF-alpha by negatively regulating its expression in various tissues such as liver and macrophages, and also by counteracting its effects. Inhibits endothelial NF-kappa-B signaling through a cAMP-dependent pathway. May play a role in cell growth, angiogenesis and tissue remodeling by binding and sequestering various growth factors with distinct binding affinities, depending on the type of complex, LMW, MMW or HMW.
SIZE: 244 amino acids; 26414 Da
SUBUNIT: Homomultimer. Forms trimers, hexamers and 12- to 18-mers. The trimers (low molecular weight complexes / LMW) are assembled via non-covalent interactions of the collagen-like domains in a triple helix and hydrophobic interactions within the globular C1q domain. Several trimers can associate to form disulfide-linked hexamers (middle molecular weight complexes / MMW) and larger complexes (higher molecular weight / HMW). The HMW-complex assembly may rely aditionnally on lysine hydroxylation and glycosylation. LMW, MMW and HMW complexes bind to HBEGF, MMW and HMW complexes bind to PDGFB, and HMW complex binds to FGF2.
SUBCELLULAR LOCATION: Secreted.
TISSUE SPECIFICITY: Synthesized exclusively by adipocytes and secreted into plasma.DOMAIN:SwissProt: Q15848 The C1q domain is commonly called the globular domain.
PTM: Hydroxylated Lys-33 was not identified in PubMed:16497731, probably due to poor representation of the N-terminal peptide in mass fingerprinting. & HMW complexes are more extensively glycosylated than smaller oligomers. Hydroxylation and glycosylation of the lysine residues within the collagene-like domain of adiponectin seem to be critically involved in regulating the formation and/or secretion of HMW complexes and consequently contribute to the insulin- sensitizing activity of adiponectin in hepatocytes (By similarity). & O-linked glycans consist of Glc-Gal disaccharides bound to the oxygen atom of post-translationally added hydroxyl groups. & Not N-glycosylated.
DISEASE: SwissProt: Q15848 # Defects in ADIPOQ are the cause of adiponectin deficiency [MIM:605441]. The result is a very low concentration RT

ALX-201-304-C050   Adiponectin (Globular Domain)(human) (recombinant) (His)   品牌 Alexis

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Adiponectin (Globular Domain)(human) (recombinant) (His)

货号： ALX-201-304-C050      产品名称： Adiponectin (Globular Domain)(human) (recombinant) (His)   品牌： Alexis 规格： 50.micro.g 三周 生化实验

Adiponectin (globular domain) (human), (recombinant) (His-tag) 
Product Specification
Alternative Name: Adipocyte complement related protein of 30kDa, ACRP30, AdipoQ, apM1, GBP28
Source: Produced in E. coli. The globular domain of human adiponectin (aa 104-244) is fused at the C-terminus to a His-tag.
UniProt ID: Q15848
Concentration: 1mg/ml
Formulation: Liquid. 0.2µm-filtered solution in 30mM TRIS-Cl, pH 8.5.
Purity: ≥95% (SDS-PAGE)
Endotoxin Content: <1EU/µg protein (LAL test; Bio Whittaker).
Biological Activity: Effect on phosphorylation of acetyl-CoA carboxylase (ACC) in C2C12 cells (see Fig. 2).
Long Term Storage: -20°C
Use/Stability: Working aliquots are stable for up to 3 months when stored at -20°C.
Handling: Avoid freeze/thaw cycles. After opening, prepare aliquots and store at -20°C.

-20°C

ALX-201-305-C050   Adiponectin (Globular Domain)(human) (recombinant)   品牌 Alexis

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Adiponectin (Globular Domain)(human) (recombinant)

货号： ALX-201-305-C050      产品名称： Adiponectin (Globular Domain)(human) (recombinant)   品牌： Alexis 规格： 50.micro.g 三周 生化实验

Adiponectin (globular domain) (mouse), (recombinant) (His-tag)
Product Specification
Alternative Name: Adipocyte complement related protein of 30kDa, ACRP30, AdipoQ, apM1, GBP28
Source: Produced in E. coli. The globular domain of mouse adiponectin (aa 104-247) is fused at the C-terminus to a His-tag.
UniProt ID: Q60994
Concentration: 1mg/ml
Formulation: Liquid. 0.2µm-filtered solution in 30mM TRIS-Cl, pH 8.5.
Purity: ≥90% (SDS-PAGE)
Endotoxin Content: <1EU/µg protein (LAL test; Bio Whittaker).
Biological Activity: Induces the phosphorylation of acetyl-CoA carboxylase (ACC) in C2C12 cells.
Long Term Storage: -20°C
Use/Stability: Working aliquots are stable for up to 3 months when stored at -20°C.
Handling: Avoid freeze/thaw cycles. After opening, prepare aliquots and store at -20°C.

-20°C

ALX-201-324-C050   Adiponectin (Globular Domain) (rat) (recombinant)(His)   品牌 Alexis

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Adiponectin (Globular Domain) (rat) (recombinant)(His)

货号： ALX-201-324-C050      产品名称： Adiponectin (Globular Domain) (rat) (recombinant)(His)   品牌： Alexis 规格： 50.micro.g 三周 生化实验

Adiponectin (globular domain) (rat), (recombinant) (His-tag)
Product Specification
Alternative Name: GBP28, Adipocyte complement related protein of 30kDa, AdipoQ, apM1, ACRP30
Source: Produced in E. coli. The globular domain of rat adiponectin is fused at the C-terminus to a His-tag.
UniProt ID: Q8K3R4
Concentration: 1mg/ml
Formulation: Liquid. 0.2-µm filtered solution in 30mM TRIS-HCl, pH 8.5.
Purity: ≥90% (SDS-PAGE)
Endotoxin Content: <1EU/µg protein (LAL test).
Long Term Storage: -20°C
Use/Stability: Working aliquots are stable for up to 3 months when stored at -20°C.
Handling: Avoid freeze/thaw cycles. After opening, prepare aliquots and store at -20°C.

-20°C