现货|ATP Colorimetric/Fluorometric Assay Kit

货号： K354-100      产品名称： 现货|ATP Colorimetric/Fluorometric Assay Kit   品牌： Biovision 规格： 100 assays 现货 生化实验

ATP Colorimetric/Fluorometric Assay Kit
Product Overview
Kit Summary:
• Detection method- Absorbance (570 nm) or Fluorescence (Ex/Em 535/587 nm)
• Sample type- Cell and Tissue lysates, culture media, urine, plasma and serum, as well as many other biological fluids
• Species reactivity- Mammalian
• Applications- The assay can detect as low as 50 picomol (1 µM) of ATP in various samples.
Features & Benefits:
• Simple procedure; takes less than 1 hour
• Fast and convenient
• Kit is designed to be a robust method which utilizes the phosphorylation of glycerol to generate a product that is easily quantified.
Kit components:
• ATP Assay Buffer
• ATP Probe (in DMSO)
• ATP Converter
• Developer Mix (lyophilized)
• ATP Standard (1 µmol; lyophilized)
Description:
ATP is the primary energy currency of living systems. Virtually all energy requiring processes utilize the chemical energy stored in the phosphate bond of ATP. ATP is formed exclusively in the mitochondria and a variety of genetic diseases can affect ATP formation in the mitochondria. There are a number of commercially available ATP assays which detects femtomoles or less of ATP by measuring luminescence (BioVision Kit 254-200, for example) but these kits require specialized luminescence instrumentation and utilize luciferase which can be difficult to maintain in active form. BioVision newly developed ATP Colorimetric and Fluorometric Assay kit is designed to be a robust, simple method which utilizes the phosphorylation of glycerol to generate a product that is easily quantified by colorimetric (λmax = 570 nm) or fluorometric (Ex/Em = 535/587 nm) methods. The assay can detect as low as 50 picomol (1 µM) of ATP in various samples. The kit provides sufficient reagents for 100 assays.
Storage Conditions:
-20°C
Shipping Conditions:
gel pack
USAGE: For Research Use Only! Not For Use in Humans.

-20°C

Coenzyme A (CoA) Assay Kit

货号： K367-100      产品名称： Coenzyme A (CoA) Assay Kit   品牌： Biovision 规格： 100 assays 3周到货 生化实验

Coenzyme A (CoA) Colorimetric/Fluorometric Assay Kit
Product Overview
Kit Summary:
• Detection method- Absorbance (570 nm) or Fluorescence (Ex/Em 535/587 nm)
• Sample type- Cell and Tissue lysates, culture media, urine, plasma and serum, as well as many other biological fluids
• Species reactivity- Mammalian
• Applications- The assay can detect 0.1 to 10 nmol of CoA (2.5-250 µM concentration range) in a variety of samples.
Features & Benefits:
• Simple procedure; takes less than 1 hour to complete the assay
• Fast and convenient
• The assay is sensitive, stable and high-throughput adaptable
Kit components:
• CoA Assay Buffer
• OxiRed Probe
• OxiRed (in DMSO)
• Conversion Enzyme Mix
• CoA Substrate
• Acyl CoA Developer
• CoA Standard (10 µmol)
Description:
Coenzyme A (CoA) is composed of units derived from cysteine, pantothenic acid, and ATP. It plays important roles in the synthesis and oxidation of fatty acids, pyruvate oxidation in the citric acid cycle and many other biological processes. One of the main functions of CoA is the carrying and transfer of acyl groups. One of the most important acyl groups transferred is the acetate group, in which case the molecule is called acetyl-CoA. The acetyl group eventually finds itself incorporated into a variety of molecules such as cholesterol, acetylcholine, melatonin, heme and the TCA cycle intermediates. BioVision has developed an easy, accurate assay to measure the CoA level in variety biological samples. In the assay, free CoA is specifically utilized to generate products which react with OxiRed Probe to generate color (λ = 570 nm) and fluorescence (Ex=535/Em=587 nm). The assay can detect 0.1 to 10 nmol of CoA (2.5-250 µM concentration range) in a variety of samples.
Storage Conditions:
-20°C
Shipping Conditions:
gel pack
USAGE: For Research Use Only! Not For Use in Humans.

-20°C

Urea Assay Kit

货号： K375-100      产品名称： Urea Assay Kit   品牌： Biovision 规格： 100 assays 3周到货 生化实验

Urea Colorimetric Assay Kit
Product Overview
Kit Summary:
• Detection method- Absorbance (570 nm)
• Sample type- Cell and Tissue lysates, culture media, urine, plasma and serum, as well as many other biological fluids
• Species reactivity- Mammalian
• Applications- The kit can detect as low as 0.5 nmol per well or 10 µM of Urea
Features & Benefits:
• Simple procedure; takes ~ 1 hour
• Fast and convenient
• The assay is sensitive, stable and high-throughput adaptable.
Kit components:
• Urea Assay Buffer
• OxiRed Probe in DMSO
• Enzyme Mix (lyophilized)
• Developer
• Converting Enzyme
• Urea Standard (100mM)
Description:
Urea is a waste product which produced in the liver, dissolved in blood (in a concentration of 2.5 – 7.5 mM), and secreted by the kidneys. Urea also plays a very important role in protein catabolism, removal of toxic ammonia from the body, and the countercurrent system which allows for reabsorption of water and critical ions in the nephrons. Urea determination is very useful for the medical clinician to assess kidney and other organs function of patients. BioVision’s Urea Assay Kit provides a rapid, simple, sensitive, and reliable for measurement of Urea level in a variety of samples such as serum, plasma, and urine, etc. In the assay, Urea reacts as substrate with compounds in the presence of enzymes to form a product that reacts with the OxiRed probe to generate color (λmax=570nm). The optical density of produced color has a direct relationship with Urea concentration in the solution. The kit can detect as low as 0.5 nmol per well or 10 µM of Urea. The assay is also suitable for high throughput studies.
Storage Conditions:
-20°C
Shipping Conditions:
gel pack
USAGE: For Research Use Only! Not For Use in Humans.

-20°C

Zinc Assay Kit

货号： K387-100      产品名称： Zinc Assay Kit   品牌： Biovision 规格： 100 assays 3周到货 生化实验

Zinc Colorimetric Assay Kit
Product Overview
 Kit Summary:
• Detection method- Absorbance (560 nm)
• Sample type- Cell and Tissue lysates, culture media, urine, plasma and serum, as well as many other biological fluids
• Species reactivity- Mammalian
• Applications- The kit can detect 0.2 µg/ml (~1-3 µM)
Features & Benefits:
• Simple procedure; takes ~ 15 minutes
• Fast and convenient
• The assay is sensitive, stable and high-throughput adaptable
Kit components:
• Zinc Reagent -1
• Zinc Reagent -2
• TCA (7%)
• Zinc Standard (50 mM)
Description:
Zinc, a metallic chemical element, symbol Zn and atomic number 30 is chemically similar to Magnesium due to its similar size and sole oxidation state of ²ᶧ. Zinc is an essential mineral of great biological significance, because many enzymes require it as an essential cofactor. Examples of zinc’s biological roles include signal transduction, gene expression, regulation of apoptosis, synaptic plasticity and prostate gland function. BioVision’s Zinc Assay Kit is a convenient colorimetric assay in which Zinc binds to a ligand with development of absorbance at 560 nm. The assay can be used with biological samples such as serum, plasma, csf or urine with detection sensitivity 0.2 µg/ml (~1-3 µM).
Storage Conditions:
+4°C
Shipping Conditions:
gel pack
USAGE: For Research Use Only! Not For Use in Humans.

+4°C

Phosphate Colorimetric Assay Kit

货号： K410-500      产品名称： Phosphate Colorimetric Assay Kit   品牌： Biovision 规格： 500 assays 3周到货 生化实验

Phosphate Colorimetric Assay Kit
Product Overview
Kit Summary:
• Detection method- Absorbance (650 nm)
• Sample type- Cell and Tissue lysates, culture media, urine, plasma and serum, as well as many other biological fluids, algal blooms and water from run-off areas of high fertilizer use
• Species reactivity- Mammalian
• Applications- Phosphate concentrations between 1 µM and 1 mM, with a lower limit of detection of approximately 0.1 nmol, can be directly determined.
Features & Benefits:
• Simple procedure; takes ~ 30 minutes
• Fast and convenient
• The assay is sensitive, stable and high-throughput adaptable
Kit components:
• Phosphate Reagent
• Phosphate Standard (10 mM)
Description:
Phosphate is one of the most important of the inorganic ions in biological systems. It functions in a variety of roles. One of the most important roles is as a molecular switch, turning enzyme activity on and off through the mediation of the various protein kinases and phosphatases in biological systems. Phosphate is also of great importance in mineralization processes and is a primary stimulus of algal blooms frequently found in bodies of fresh water, due to run-off from areas of high fertilizer use. The newly designed Phosphate Colorimetric Assay Kit provides an easy, quick and sensitive means of assessing phosphate over a wide range of concentrations. The assay utilizes a proprietary formulation of malachite green and ammonium molybdate which forms a chromogenic complex with phosphate ion giving an intense absorption band around 650 nm. Phosphate concentrations between 1 µM and 1 mM, with a lower limit of detection of approximately 0.1 nmol, can be directly determined. The Phosphate Colorimetric Assay Kit provides 500 assays using microtiter plates or 100 assays using 1 ml cuvettes.
Storage Conditions:
Room temperature
Shipping Conditions:
Room Temperature
USAGE: For Research Use Only! Not For Use in Humans.

RT

RBP4 (human) Competitive ELISA Kit

货号： K4911-100      产品名称： RBP4 (human) Competitive ELISA Kit   品牌： Biovision 规格： 100 assays 3周到货 生化实验

RBP4 (human) Competitive ELISA Kit
Product Overview
Assay Kit Name:
RBP4 (human) Competitive ELISA Kit
Kit Summary:
Detection method- Absorbance (450 nm)
• Sample type- Serum, urine, or cell culture supernatant
• Species reactivity- Mammalian
• Applications- This assay detects human RBP4 in the range of 0.001 – 5 µg RBP4/ml and a detection limit of 1 ng/ml (based on adding two standard deviations to the mean value of the (50) zero standards).
Features & Benefits:
• Simple procedure
• Fast and convenient
• High-throughput adaptable
Kit Components:
• Pre-coated Microtiter Plate
• Wash Buffer (10X)
• Diluent (5X)
• Detection Antibody
• Detector 100X (Hrp conjugated anti-IgG)
• Human RBP4 Standard (lyophilized, 5 μg)
• Human RBP4 QC Sample (lyophilized)
• Substrate Solution I (TMB)
• Substrate Solution II (Peroxidase)
• Stop Solution
• Plate Sealers
Description:
Retinol binding protein (RBP) 4 is the only specific transport protein for vitamin A in the circulation whose function is to deliver vitamin to target tissues. In obesity and type 2 diabetes, the expression of Glut4 is significantly impaired in adipocytes. Glucose transport via Glut4 is the rate-limiting step for glucose use by muscle and adipose tissue. Adipocyte-specific deletion of Gluts leads to notable elevation of mouse RBP4 causing systemic insulin resistance, and that reduction of RBP4 improves insulin resistance. This identified a novel role of RBP4 in regulating insulin action and RBP4 is recorded as an adipocyte-derived hormone. The RBP4 (human) ELISA Kit is to be used for the in vitro quantitative determination of human RBP4 in serum, urine and cell culture supernatant. This assay is a competitive ELISA which utilizies a 96-well microtiter plate which was pre-coated with a human RBP4. A purified polyclonal which recognizing native human RBP4 reacts with a series of predetermined recombinant human RBP4 standard proteins or the test samples under competition in the human RBP4-coated plate. Their relative reactivity is plotted with that of the standard proteins. This ELISA is specific for the measurement of natural and recombinant human RBP4. It does not cross-react with mouse RBP4, rat RBP4, human adiponectin, rat adiponectin, human resistin, human vaspin, human clusterin, human leptin, human IL-23, human IL-33, human GPX3, human Nampt, human ANG1, human ANG2, human ANGPTL3, human ANGPTL4, human ANGPTL6, human FABP4, human RELM-β, rat RELM-α, mouse Nampt, human PAI-1. The assay range is 0.001 – 5 µg RBP4/ml and a detection limit of 1 ng/ml (based on adding two standard deviations to the mean value of the (50) zero standards).
Storage Conditions:
+4°C
Shipping Conditions:
gel pack
USAGE: For Research Use Only! Not For Use in Humans.

+4°C

ANGPTL6 (human) Serum ELISA Kit

货号： K4916-100      产品名称： ANGPTL6 (human) Serum ELISA Kit   品牌： Biovision 规格： 100 assays 3周到货 生化实验

ANGPTL6 (human) Serum ELISA Kit
Product Overview
Assay Kit Name:
ANGPTL6 (human) Serum ELISA Kit
Kit Summary:
Detection method- Absorbance (450 nm)
• Sample type- Cell and tissue culture supernatants, urine, plasma, serum, as well as many other biological fluids
• Species reactivity- Mammalian
• Applications- This assay detects human ANGPTL6 in the range of 1.56 – 100 ng/ml ANGPTL6/ml. The lowest level of ANGPTL6 that can be detected by this assay is 1.2 ng/ml.
Features & Benefits:
• Simple procedure
• Fast and convenient
• High-throughput adaptable
Kit Components:
• 1 plate coated with human ANGPTL6 Antibody
• Wash Buffer 10X
• Diluent 5X
• Detection Antibody
• Detector 100X (HRP Conjugated anti-rabbit IgG)
• human ANGPTL6 Standard (lyophilized)
• human ANGPTL6 QC sample (lyophilized)
• Substrate Solution I (TMB)
• Substrate Solution II (Peroxidase)
• Stop Solution
• 3 plate sealers (plastic film)
Description:
Seven angiopoietin-like proteins (ANGPTLs) share the characteristic protein structure of the angiopoietin family (ANG), but differ in their inability to bind antiopoietin receptor, Tie-2. ANGPTL6 was originally named angiopoietin-related growth factor (AGF) having an N-terminal coiled-coil-like domain and a C-terminal fibrinogen-like domain, both of which are conserved in ANG. It is a circulating protein secreted by liver that induces angiogenesis by direct effect of epidermal ANGPTL6 on endothelial cells and proliferation of skin cells, and thereby promotes wound healing. 80% of Angptl6 -/- mice died at about embryonic day 13. The surviving null mice developed marked obesity, lipid accumulation in skeletal muscle and liver, and insulin resistance accompanied by reduced energy expenditure relative to controls. Conversely, mice with constitutive overexpression of ANGPTL6 showed leanness and increased insulin sensitivity resulting from increased energy expenditure, and were also protected from high-fat diet-induced obesity, insulin resistance, and non-adipose tissue steatosis. This assay is a sandwich Enzyme Linked-Immunosorbent Assay (ELISA) for quantitative determination of human ANGPTL6 in biological fluids. A monoclonal antibody specific for ANGPTL6 has been precoated onto the 96-well microtiter plate. Standards and samples are pipetted into the wells for binding to the coated antibody. After extensive washing to remove unbound compounds, ANGPTL6 is recognized by the addition of a purified polyclonal antibody specific for ANGPTL6 (Detection Antibody). After removal of excess polyclonal antibody, HRP conjugated anti-rabbit IgG (Detector) is added. Following a final washing, peroxidase activity is quantified using the substrate 3,3’,5,5’-tetramethylbenzidine (TMB). The intensity of the color reaction is measured at 450 nm after acidification and is directly proportional to the concentration of ANGPTL6 in the samples. The assay range is 1.56 – 100 ng/ml ANGPTL6/ml. The lowest level of ANGPTL6 that can be detected by this assay is 1.2 ng/ml.
Storage Conditions:
+4°C
Shipping Conditions:
gel pack
USAGE: For Research Use Only! Not For Use in Humans.

+4°C