QIA128-1KIT   ULTRASENSITIVE CELL PROLIFERATION ASSAY   品牌 Merck

ULTRASENSITIVE CELL PROLIFERATION ASSAY

货号: QIA128-1KIT      产品名称: ULTRASENSITIVE CELL PROLIFERATION ASSAY   品牌: Merck 规格: *** 3周到货 生化实验

Ultrasensitive Cell Proliferation Assay Kit, Fluorogenic
A highly sensitive, reliable cell proliferation assay utilizing Calcein-AM, a fluorescent probe for staining viable cells (Ex.: ~485 nm; Em.: ~520 nm). For optimal results serum-free media or Dulbecco’s PBS is recommended. Endogenous esterases in serum can lead to increased blank values which can be subtracted out. Extremely useful for cytotoxicity assays.
产品信息
Format  96-well plate
Form  500 Tests
Detection method  Fluorometric
Protect from Light  是的
Sensitivity  100 Cells
Assay range  250-25,000 cells
Assay time  1 h
Sample type  Viable cells
Kit contains  Calcein-AM, Dulbecco-PBS.
商店和运输信息
Storage  -20°C
Ship  Blue Ice Only
Multiple Toxicity Values, refer to MSDS

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1215-100   TMB, Ultrasensitive   品牌 Biovision

TMB, Ultrasensitive

货号: 1215-100      产品名称: TMB, Ultrasensitive   品牌: Biovision 规格: 100 ml 3周到货 生化实验

TMB, Ultrasensitive
Product Overview
Product Name: TMB, Ultrasensitive
Alternate Name/Synonyms: 3,3′,5,5′-Tetramethylbenzidine; TMBUS
Description: 3,3’5,5′-Tetramethylbenzidine (TMB) has been shown to be a safe-sensitive substrate for the assay of horseradish peroxidase (HRP). Initially, in the presence of HRP and hydrogen peroxide, a one-electron oxidation product is formed. This compound, a cation free radical, is blue in color with an adsorption maximum at 653 nm. Further reaction with HRP/H₂O₂ or acidification of the radical with acid yields the diimine terminal oxidation product adsorbing light at 450 nm. The extinction coefficient of the radical (E653 nm = 3.9 x 10⁴ mo⁻¹1 cm⁻¹) and diimine (E450 nm = 5.9 x 10⁴ mol⁻¹ cm⁻¹) provide a remarkably sensitive system for the assay of HRP and HRP labeled probes. TMBUS, available from Biovision Inc., is a single component reagent stable at room temperature and not sensitive to normal laboratory light. It is optimized with respect to TMB and hydrogen peroxide concentrations and yields a linear response with the concentrations of HRP usually employed in immunologic assays.
Peptide Sequence: N/A
Appearance: Liquid
Formulation: 2.08 mMol L⁻¹ and Hydrogen Peroxide, citric acid buffer at pH 3.3.
CAS Number: 54827-17-7
Molecular Formula: C₁₆H₂₀N₂
Molecular Weight: 240.35
Purity: N/A
Solubility:N/A
Storage Temp.: +4°C
Shipping Conditions: RT
Handling: Protect from light
SMILES: CC1=CC(=CC(=C1N)C)C2=CC(=C(C(=C2)C)N)C
InChi: InChI=1S/C16H20N2/c1-9-5-13(6-10(2)15(9)17)14-7-11(3)16(18)12(4)8-14/h5-8H,17-18H2,1-4H3
InChi Key: UAIUNKRWKOVEESUHFFFAOYSA-N
PubChem CID: 41206
MDL Number: MFCD00007748
USAGE: For Research Use Only! Not For Use in Humans.

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