StayBrite Highly Stable Luciferase/Luciferin Reagent

货号： K790-10000      产品名称： StayBrite Highly Stable Luciferase/Luciferin Reagent   品牌： Biovision 规格： 10000 assays 3周到货 生化实验

StayBrite™ Highly Stable Luciferase/Luciferin Reagent
Product Overview
Kit Summary:
• Detection method- Luminescence
• Sample type- Cell and Tissue culture homogenates, fermentation broth
• Species reactivity- Mammalian
• Kit size- Convenient sizes (100, 1000 and 10,000 assays)
• Application- The activity profile shows a linear detection range for ATP values as low as 10 fmol/assay (1 nM).
Features & Benefits:
• Simple procedure; takes ~ 20-30 minutes
• Fast and convenient
• Stable for >10 hours at room temperature and >60 minutes at 37°C.
Kit components:
• StayBrite™ Reconstitution Buffer
• rLucHS Reagent Mix
Description:
BioVision’s Highly Stable Luciferase/Luciferin Reagent utilizes a genetically modified variant derived from the Luciferase of Diaphanes pectinealis (Chinese Firefly) endemic to Yunnan province, China. BioVision’s luciferase (rLucHS) has been modified to provide enhanced stability compared to the normal phenotype of Photinus pyralis, as well as a broader and more physiologically relevant effective pH range. At all pHs below ~8.2, rLucHS is significantly more active than commercial Photinus pyralis luciferase and is stable for weeks at room temperature and >60 minutes at 37°C. The specific activity of rLucHS is ~ 5×10ᶦᶦRLU/mg protein. This reagent has application to measure ATP levels in a wide variety of biological samples such as cells, tissues and fermentation broth, etc.
Storage Conditions:
-80°C
Shipping Conditions:
gel pack
USAGE: For Research Use Only! Not For Use in Humans.

-80°C

StayBrite Highly Stable ATP Assay Kit

货号： K791-100      产品名称： StayBrite Highly Stable ATP Assay Kit   品牌： Biovision 规格： 100 assays 3周到货 生化实验

StayBrite™ Highly Stable ATP Bioluminescence Assay Kit
Product Overview
Kit Summary:
• Detection method- Luminescence
• Sample type- Cell and Tissue culture homogenates
• Species reactivity- Mammalian
• Kit size- Convenient sizes (100 and1000 assays)
• Application- The activity profile shows a linear detection range for ATP values as low as 10 fmol/assay (1 nM).
Features & Benefits:
• Simple procedure; takes ~ 20-30 minutes
• Fast and convenient
• Stable for >10 hours at room temperature and >60 minutes at 37°C.
• The stable reagent provides more accuracy than any other ATP assay kits.
Kit components:
• StayBrite™ Reaction Buffer
• StayBrite™ Reconstitution Buffer
• StayBrite™ Enzyme Mix (lyophilized)
• ATP Standard (MW 551) (lyophilized)
Description:
Adenosine-5’-triphosphate (ATP) plays an important role in living systems. It has also been used as an indicator to monitor many biological processes and disease stages, etc.. An accurate and reliable detection of ATP level has wide applications. Luciferase/Luciferin has been used to sensitively measure ATP levels. Conventional Luciferase/Luciferin ATP detection systems, however, are unstable. Luciferase loses activity rapidly. At BioVision, we have developed a highly stable Luciferase, which is a genetically modified variant derived from the Luciferase of Diaphanes pectinealis (Chinese Firefly) endemic to Yunnan province, China. We named the highly stable luciferase rLucHS. Compared to the normal phenotype of Photinus pyralis, the rLucHS provides enhanced stability, excellent sensitivity, and a broader and more physiologically relevant effective pH range. At all pH’s below ~8.2, rLucHS is significantly more active than Photinus luciferase and is stable for weeks at room temperature and >60 minutes at 37°C. The activity profile shows a linear detection range for ATP values as low as 10 fmol/assay (1 nM). The specific activity of rLucHS is ~ 5×10ᶦᶦRLU/mg protein. The assay can be fully automated for high throughput (1 second/sample) and is extremely sensitive. The high sensitivity of this assay is ideal for detecting ATP production or consumption in various enzymatic reactions.
Storage Conditions:
-20°C
Shipping Conditions:
gel pack
USAGE: For Research Use Only! Not For Use in Humans.

-20°C