标签归档：novobiocin

C9461   Novobiocin Sodium Salt   品牌 Promega

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Novobiocin Sodium Salt

货号： C9461      产品名称： Novobiocin Sodium Salt   品牌： Promega 规格： 1g 二周到货 生化试验

说明 
 Regulated Mammalian Expression System ( 可控哺乳动物表达系统) 的特点在于本底低，可被迅速强烈地诱导，可下调哺乳动物细胞中的基因表达。可控哺乳动物表达系统基于新的开/ 关系统，开关通过嵌合的反式激活蛋白香豆素相关化合物进行迅速和灵敏的调控。纳摩尔浓度的抗生素香豆霉素即可引发嵌合反式激活蛋白同型二聚体的形成，接着与位于驱动转录目标蛋白编码序列的最小启动子上游的λ 操作子序列结合。蛋白表达水平可以通过调节香豆霉素的浓度来控制。更重要的是， 这一表达可以通过加入新生霉素而被迅速有效地关闭，新生霉素作为拮抗剂，可将反式激活蛋白二聚体解离。可控哺乳动物表达系统之所以适合基因表达的体内调控，在于引入哺乳动物细胞中的两种特别设计的质粒以及直接加入到培养基中的调控化合物－香豆霉素和新生霉素。
目的蛋白编码区被克隆进pF12A RM Flexi® 载体或pF12K RM Flexi® 载体，都是专门用于可控哺乳动物(RM) 的蛋白表达。这些载体整合有蛋白编码区上游的调控启动子序列，并且与Flexi® 载体系统兼容。以瞬时转染为例，含有目标蛋白编码区的pF12A 或pF12K RM Flexi® 载体与pReg neo 载体共转染进入哺乳动物细胞。在应答香豆霉素和新生霉素的调控过程中，pReg neo 载体被设计为表达嵌合反式激活蛋白，该蛋白可与pF12A 或pF12K RM Flexi® 载体的调控启动子区域发生作用。另外，在抗生素G-418 存在的条件下，pReg neo 载体编码的新霉素磷酸转移酶基因可允许稳定并转染细胞筛选和增殖中保持稳定。
特点   . 更好的数据：诱导表达高度可控，本底蛋白表达水平低。
• 表达水平可控：蛋白表达是剂量反应性的；蛋白表达的开启/ 关闭控制迅速而灵敏。
• 通用：与其它Flexi® 载体相兼容。

 

N6160   Novobiocin sodium|新生霉素|现货   品牌 Sigma

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Novobiocin sodium|新生霉素|现货

货号： N6160      产品名称： Novobiocin sodium|新生霉素|现货   品牌： Sigma 规格： 100mg 现货

英文名称：Novobiocin sodium
中文名称：新生霉素 钠
meets USP testing specifications
别名: 新生霉素 钠盐
CAS号 1476-53-5
经验分子式（希尔表示法） C31H35N2NaO11
分子量 634.61
suitability  meets USP testing specifications 
storage temp. 2-8°C
说明：
Application
For the production of positively supercoiled plasmid DNA.1 Inhibitor of bacterial DNA gyrase and eukaryotic DNA topoisomerase.2 Inhibitor of retrovirus RNA-dependent DNA-polymerase.3
Biochem/physiol Actions
Mode of Action: Inhibits DNA synthesis by inhibiting the enzyme Topoisomerase II.
Antimicrobial spectrum: Gram-positive bacterial.
本产品仅用于生命科学研究实验。

2-8°C

N6160   现货|新生霉素|Novobiocin sodium|Novobiocin sodium salt   品牌 Sigma

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现货|新生霉素|Novobiocin sodium|Novobiocin sodium salt

货号： N6160      产品名称： 现货|新生霉素|Novobiocin sodium|Novobiocin sodium salt   品牌： Sigma 规格： 1G 大量现货

中文名：新生霉素
英文名：Novobiocin; Cathocin; Albamycin；Novobiocin sodium salt；Novobiocin sodium
用途：抗菌作用与青霉素G、红霉素相似，主要抗革兰氏阳性菌，金葡菌的最小抑菌度MIC一般为1～4μg/ml，对耐青霉素的金葡菌或对其他抗生素已产生耐药性的菌株依旧敏感。对革兰氏阴性菌除嗜血杆菌外，大都不敏感，对立克次体、真菌、病毒无效。
SIGMA相关资料如下：
Sigma-Aldrich  N6160  Novobiocin sodium
meets USP testing specifications
Synonym: Novobiocin sodium salt
CAS Number: 1476-53-5
Linear Formula: C31H35N2NaO11
Molecular Weight: 634.61
Beilstein Registry Number: 3892910
EC Number: 216-023-6
MDL number: MFCD00066541
PubChem Substance ID: 24897774
Description
Application For the production of positively supercoiled plasmid DNA.1 Inhibitor of bacterial DNA gyrase and eukaryotic DNA topoisomerase.2 Inhibitor of retrovirus RNA-dependent DNA-polymerase.3
Biochem/physiol Actions Mode of Action: Inhibits DNA synthesis by inhibiting the enzyme Topoisomerase II.
Antimicrobial spectrum: Gram-positive bacterial.
性质
suitability meets USP testing specifications
storage temp. 2-8°C

2-8°C

N5986-1 g   Novobiocin sodium salt   品牌 LKT

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Novobiocin sodium salt

货号： N5986-1 g      产品名称： Novobiocin sodium salt   品牌： LKT 规格： 1 g 三周到货 生化试验

Novobiocin sodium salt
Description
Novobiocin is an antibiotic and inhibitor of HSP90. Also inhibits DNA gyrase and retrovirus RNA-dependent DNA polymerase. Useful for the production of positively supercoiled plasmid DNA.
Specifications 
Cas No. 1476-53-5
Product ID  N5986
Product Name  Novobiocin sodium salt
Formula C31H35N2NaO11
Formula Wt. 634.61
References Pick A, Klinkhammer W, Wiese M. Chem Med Chem. 9:1498-505 (2010). Lockshon D, Morris DR. Nucleic Acids Res. 10:2999-3017 (1983).
 

N5986-5 g   Novobiocin sodium salt   品牌 LKT

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Novobiocin sodium salt

货号： N5986-5 g      产品名称： Novobiocin sodium salt   品牌： LKT 规格： 5 g 三周到货 生化试验

Novobiocin sodium salt
Description
Novobiocin is an antibiotic and inhibitor of HSP90. Also inhibits DNA gyrase and retrovirus RNA-dependent DNA polymerase. Useful for the production of positively supercoiled plasmid DNA.
Specifications 
Cas No. 1476-53-5
Product ID  N5986
Product Name  Novobiocin sodium salt
Formula C31H35N2NaO11
Formula Wt. 634.61
References Pick A, Klinkhammer W, Wiese M. Chem Med Chem. 9:1498-505 (2010). Lockshon D, Morris DR. Nucleic Acids Res. 10:2999-3017 (1983).
 

BML-A256-0001   Novobiocin sodium   品牌 Biomol

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Novobiocin sodium

货号： BML-A256-0001      产品名称： Novobiocin sodium   品牌： Biomol 规格： 1 g 3周到货 生化试验

Replaces Prod. #: ALX-380-093
Antibiotic. Inhibitor of HSP90. Interacts with the C-terminal ATP-binding domain of HSP90 in contrast to the benzoquinone ansamycins 17-AAG (Prod. No. BML-EI308), geldanamycin (Prod. No. BML-EI280), herbimycin A (Prod. No. BML-EI227) and the chemically unrelated radicicol (Prod. No. BML-EI285), which bind to the N-terminal ATP-binding site of HSP90. Inhibitor of DNA gyrase. Useful for the production of positively supercoiled plasma DNA, targeting the nucleotide-binding site of gyrase B. Inhibits retrovirus RNA-dependent DNA polymerase. Potent inhibitor of ADP ribosylation. Inhibits LPS-induced production of pro-inflammatory cytokines, such as TNF-α, IL-1, IL-6 and IL-10. Inhibits protein synthesis and alters the phosphorylation state of several cytosolic proteins. Reverses etoposide resistance in non-P-glycoprotein expressing multidrug resistant (MDR) tumor cell lines. Inducer of CD38 on cells of the myelomonocytic lineage. Forms ion channels in lipid bilayers. Inhibitor of breast cancer resistance protein (BCRP)
Product Specification
Alternative Name: Albamycin, Streptonivicin, N-[7-[[3-O-Aminocarbonyl-6-deoxy-5-C-methyl-4-O-β-L-lyxo-hexapyranosyl]oxy]-4-hydroxy-8-methyl-2-oxo-2H-1-benzopyran-3-yl]-4-hydroxy-3-(3-methyl-2-butenyl)benzamide  
Formula: C31H35N2O11 . Na  
MW: 611.6 . 23.0  
CAS: 1476-53-5  
MI: 14: 6722  
RTECS: RD5425000  
Purity: ≥90%   
Appearance: White solid.  
Hazard: HARMFUL.  
Solubility: Soluble in water (100mg/ml), ethanol or DMSO.  
Long Term Storage: -20°C  
Handling: Protect from light.

-20°C

BML-A256-0005   Novobiocin sodium   品牌 Biomol

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Novobiocin sodium

货号： BML-A256-0005      产品名称： Novobiocin sodium   品牌： Biomol 规格： 5 g 3周到货 生化试验

Replaces Prod. #: ALX-380-093
Antibiotic. Inhibitor of HSP90. Interacts with the C-terminal ATP-binding domain of HSP90 in contrast to the benzoquinone ansamycins 17-AAG (Prod. No. BML-EI308), geldanamycin (Prod. No. BML-EI280), herbimycin A (Prod. No. BML-EI227) and the chemically unrelated radicicol (Prod. No. BML-EI285), which bind to the N-terminal ATP-binding site of HSP90. Inhibitor of DNA gyrase. Useful for the production of positively supercoiled plasma DNA, targeting the nucleotide-binding site of gyrase B. Inhibits retrovirus RNA-dependent DNA polymerase. Potent inhibitor of ADP ribosylation. Inhibits LPS-induced production of pro-inflammatory cytokines, such as TNF-α, IL-1, IL-6 and IL-10. Inhibits protein synthesis and alters the phosphorylation state of several cytosolic proteins. Reverses etoposide resistance in non-P-glycoprotein expressing multidrug resistant (MDR) tumor cell lines. Inducer of CD38 on cells of the myelomonocytic lineage. Forms ion channels in lipid bilayers. Inhibitor of breast cancer resistance protein (BCRP)
Product Specification
Alternative Name: Albamycin, Streptonivicin, N-[7-[[3-O-Aminocarbonyl-6-deoxy-5-C-methyl-4-O-β-L-lyxo-hexapyranosyl]oxy]-4-hydroxy-8-methyl-2-oxo-2H-1-benzopyran-3-yl]-4-hydroxy-3-(3-methyl-2-butenyl)benzamide  
Formula: C31H35N2O11 . Na  
MW: 611.6 . 23.0  
CAS: 1476-53-5  
MI: 14: 6722  
RTECS: RD5425000  
Purity: ≥90%   
Appearance: White solid.  
Hazard: HARMFUL.  
Solubility: Soluble in water (100mg/ml), ethanol or DMSO.  
Long Term Storage: -20°C  
Handling: Protect from light.

-20°C