NA/H EXCHANGER (NHE1), RBX-50UG

货号： AB3081      产品名称： NA/H EXCHANGER (NHE1), RBX-50UG   品牌： Millipore 规格： EA 三周到货 生化实验

Anti-Na+/H+ Exchanger-1 Antibody
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REFERENCES
Malignant gliomas display altered plasma membrane potential and pH regulation–interaction with Tc-99m-MIBI and Tc-99m-Tetrofosmin uptakes.
N Perek, D Denoyer, F Dubois, F Koumanov (2002) General physiology and biophysics.21:381-404
Hormonal regulation, pharmacology, and membrane sorting of vertebrate Na+/H+ exchanger isoforms.
Noël, J and Pouysségur, J (1995) Am. J. Physiol., 268: C283-96 (1995)
Reconstitution of Na+/H(+)-antiporter of bovine renal brush-border membrane into proteoliposomes and detection of a 110 kDa protein cross-reactive with antibodies against a human Na+/H(+)-antiporter partial peptide in antiport-active fractions after partial fractionation.
Yamashita, T and Kawakita, M (1992) J. Biochem., 111: 162-7 (1992)
Mammalian Na+/H+ exchanger gene family: structure and function studies.
Yun, C H, et al. (1995) Am. J. Physiol., 269: G1-11 (1995)
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Species Reactivity Key Applications Host Format Antibody Type
B, H, M, Po, R  ELISA, WB Rabbit Affinity Purified Polyclonal Antibody
Description:
Anti-Na+/H+ Exchanger-1 Antibody
Promotional Text:
Special Shipping Offer on Antibodies
100% Performance Guaranteed
Trade Name:
Chemicon (Millipore)
Specificity:
Recognizes Na+/H+ Exchanger 1 (NHE1). The immunogen peptide shows no significant homology with other NHE isoforms (NHE2-5).
Molecular Weight:
91 kDa
Immunogen:
A 22 aa peptide within the cytoplasmic, C-terminal domain of rat NHE1, coupled to KLH.
Species Reactivity:
Bovine
Human
Mouse
Pig
Rat
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Application Notes:
Western blot: 1-10 μg/mL using Chemiluminescence technique.
ELISA: 1:100,000 using 50-100 ng control peptide per well.
Optimal working dilutions must be determined by end user.
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Control:
Kidney tissue (basolateral membrane)
Purification Method:
Immunoaffinity purified
Presentation:
Affinity Purified immunoglobulin. Liquid in 100 mM Tris, pH 7.5 and 0.2% BSA and 0.05% sodium azide.
Storage Conditions:
Maintain for 1 year at -20°C from date of shipment. Aliquot to avoid repeated freezing and thawing. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
UniProt Number:
P19634
Entrez Gene Number:
NM_003047.2
Gene Symbol:
SLC9A1
APNH
+H
APNH1
FLJ42224
NHE-1
Na
NHE1
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Alternate Names:
NHE1
Usage Statement:
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Key Applications:
ELISA
Western Blotting
UniProt Summary:
FUNCTION: SwissProt: P19634 # Involved in pH regulation to eliminate acids generated by active metabolism or to counter adverse environmental conditions. Major proton extruding system driven by the inward sodium ion chemical gradient. Plays an important role in signal transduction.
SIZE: 815 amino acids; 90763 Da
SUBUNIT: Interacts with tescalcin, CHP and CHP2.
SUBCELLULAR LOCATION: Membrane; Multi-pass membrane protein.
TISSUE SPECIFICITY: Kidney and intestine.
PTM: Phosphorylated upon DNA damage, probably by ATM or ATR. & O-glycosylated.
SIMILARITY: SwissProt: P1963 -20℃

NA/H EXCHANGER (NHE2), RBX-50UG

货号： AB3083      产品名称： NA/H EXCHANGER (NHE2), RBX-50UG   品牌： Millipore 规格： EA 三周到货 生化实验

Anti-Na+/H+ Exchanger-2 Antibody
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REFERENCES
Increased luminal pH in the epididymis of infertile c-ros knockout mice and the expression of sodium-hydrogen exchangers and vacuolar proton pump H+-ATPase.
Ching-Hei Yeung, Sylvie Breton, Iwan Setiawan, Yaoxian Xu, Florian Lang, Trevor G Cooper (2004) Molecular reproduction and development.68:159-68
Characterization of Na(+)/H(+) antiporter activity in PC-Cl3 thyroid cells.
Sebastiano Vilella, Roberta Schiavone, Loredana Zilli, Santo Marsigliante, Carlo Storelli (2003) Cellular physiology and biochemistry : international journal of experimental cellular physiology, biochemistry, and pharmacology.13:207-14
Molecular cloning, sequencing, chromosomal localization, and tissue distribution of the human Na+/H+ exchanger (SLC9A2).
Ghishan, F K, et al. (1995) Genomics, 30: 25-30 (1995)
Hormonal regulation, pharmacology, and membrane sorting of vertebrate Na+/H+ exchanger isoforms.
Noël, J and Pouysségur, J (1995) Am. J. Physiol., 268: C283-96 (1995)
View All
Species Reactivity Key Applications Host Format Antibody Type
R  ELISA, WB Rabbit Affinity Purified Polyclonal Antibody
Description:
Anti-Na+/H+ Exchanger-2 Antibody
Promotional Text:
Special Shipping Offer on Antibodies
100% Performance Guaranteed
Trade Name:
Chemicon (Millipore)
Specificity:
C-terminal domain of the rat NHE2. The antibody is expected to work on human and mouse (20/20) and possibly rabbit (17/20) due to sequence conservation.
Immunogen:
A 20 AA peptide within the cytoplasmic, C-terminal domain of the rat NHE2 (Collins et al. 1990; Wang et al. 1993; Ghishan et al. 1995; Chris Yun et al. 1995; Josette & Pouyssegur 1995).
Background Information:
Na+/H+ exchangers (NHE) of mammalian cells are plasma membrane intrinsic proteins mediating exchange of Na+ and H+ ions in various tissues. The NHE catalyzes the electoneuronal transport of extracellular Na+ for intracellular H+. They play a major role in regulation of intracellular pH (pHi) addition to trans-cellular absorption of Na+, cell volume regulation and possibly in cell proliferation. These primary functions of the Na+/H+ exchanger have been related to many pathophysiological states, include hypertension, organ growth and hypertrophy, regression of cancer and renal intestinal disorders. Five isoforms (NHE1-5) have been cloned so far. They are all similar in their primary structure and predicted to have 10-12 transmembrane domains. The COOH-terminals of NHE1, NHE2 and NHE3 are intracellular.
NHE2 has been implicated in volume regulation in renal inner medullary collecting duct cells. Its mRNA is found in kidney medulla, cortex, colon, jejunum, ileum, human jejunum, ileum, duodenum, stomach and adrenal glands (Collins et al. 1990; Wang et al. 1993; Ghishan et al. 1995; Chris Yun et al. 1995; Josette & Pouyssegur 1995).
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Species Reactivity:
Rat
Application Notes:
Western blot: 1-10 μg/mL using Chemiluminescence technique. The molecular weight of NHE2 is approximately 90kDa (Brookstein et al., 1994).
ELISA: 1:100,000 using 50-100ng control peptide (AG784)/well.
Optimal working dilutions must be determined by end user.
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Presentation:
Liquid in PBS with 0.1% BSA
Storage Conditions:
Maintain frozen at -20°C in undiluted aliquots for up to 6 months after date of receipt. Avoid repeated freeze/thaw cycles.
During shipment, small volumes of product will occasionally become entrapped in the seal of the product vial. For products with volumes of 200 μL or less, we recommend gently tapping the vial on a hard surface or briefly centrifuging the vial in a tabletop centrifuge to dislodge any liquid -20℃

NA/H EXCHANGER (NHE3), RBX-50UG

货号： AB3085      产品名称： NA/H EXCHANGER (NHE3), RBX-50UG   品牌： Millipore 规格： EA 三周到货 生化实验

Anti-Na+/H+ Exchanger-3 Antibody
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REFERENCES
Evidence for NHE3-mediated Na transport in sheep and bovine forestomach.
Rabbani I, Siegling-Vlitakis C, Noci B, Martens H (2011) American journal of physiology Regulatory, integrative and comparative physiology.301:R313-9. Epub 2011 May 25.
Evidence for NHE3-mediated Na transport in sheep and bovine forestomach.
Rabbani I, Siegling-Vlitakis C, Noci B, Martens H (2011) American journal of physiology Regulatory, integrative and comparative physiology.301:R313-9. Epub 2011 May 25.
Evidence for NHE3-mediated Na transport in sheep and bovine forestomach.
Imtiaz Rabbani,Christiane Siegling-Vlitakis,Bardhyl Noci,Holger Martens (2011) American journal of physiology. Regulatory, integrative and comparative physiology.301
Phenol injury-induced hypertension stimulates proximal tubule Na+/H+ exchanger activity.
Patrick K K Leong, Li E Yang, Carol S Landon, Alicia A McDonough, Kay-Pong Yip (2006) American journal of physiology. Renal physiology.290:F1543-50
View All
Species Reactivity Key Applications Host Format Antibody Type
R  ELISA, WB Rabbit Affinity Purified Polyclonal Antibody
Description:
Anti-Na+/H+ Exchanger-3 Antibody
Promotional Text:
Special Shipping Offer on Antibodies
100% Performance Guaranteed
Trade Name:
Chemicon (Millipore)
Specificity:
Recognizes NHE3.
Molecular Weight:
93 kDa
Immunogen:
A 22 AA peptide within the cytoplasmic, c-terminal domain of the rat NHE3 (Brant et al. 1995; Orlowsji et al. 1992; Chris Yun et al. 1995; Josette & Pouyssegur 1995). Control peptide is available, cat# AG779.
Species Reactivity:
Rat
Species Reactivity Note:
The immunogen sequence shares 90% homology with mouse NHE3.
Application Notes:
Western blot: 1-10 μg/mL using Chemiluminescence technique. A band of approximately 90 kD has been detected in brush border membrane (Yohsioka et al. 1997). ELISA: 1:100,000 using 50-100 ng control peptide/well.
Optimal working dilutions must be determined by end user.
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Control:
Kidney, jejunum, colon, and stomach tissue
Purification Method:
Immunoaffinity purified
Presentation:
Affinity Purified immunoglobulin. Liquid in PBS with 0.1% BSA.
Storage Conditions:
Maintain for 6 months at -20°C from date of shipment. Aliquot to avoid repeated freezing and thawing. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
UniProt Number:
P48764
Entrez Gene Number:
NP_004165
Gene Symbol:
SLC9A3
Na
NHE3
MGC126720
NHE-3
+H
MGC126718
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Alternate Names:
NHE3
Usage Statement:
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
View All »
Key Applications:
ELISA
Western Blotting
UniProt Summary:
FUNCTION: SwissProt: P48764 # Involved in pH regulation to eliminate acids generated by active metabolism or to counter adverse environmental conditions. Major proton extruding system driven by the inward sodium ion chemical gradient. Plays an important role in signal transduction.
SIZE: 834 amino acids; 92908 Da
SUBUNIT: Binds SLC9A3R1 and SLC9A3R2. Interacts with SHANK2 (By similarity).
SUBCELLULAR LOCATION: Membrane; Multi-pass membrane pr -20℃

NA/H EXCHANGER (NHE4), RBX-50UG

货号： AB3087      产品名称： NA/H EXCHANGER (NHE4), RBX-50UG   品牌： Millipore 规格： EA