Anti-MYH11

货号： ABT293      产品名称： Anti-MYH11   品牌： Millipore 规格： EA 三周到货 生化实验

Anti-MYH11 Antibody
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Species Reactivity Key Applications Host Format Antibody Type
H, M  WB, IHC Rabbit Affinity Purified Polyclonal Antibody
Description:
Anti-MYH11 Antibody
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Molecular Weight:
~225 kDa observed
Immunogen:
GST-tagged recombinant protein corresponding to human MYH11.
Background Information:
Myosin-11 (MYH11) is a hexameric protein that consists of 2 heavy chain subunits, 2 alkali light chain subunits, and 2 regulatory light chain subunits. It converts chemical energy into mechanical energy through the hydrolysis of ATP. MYH11 is expressed in the umbilical artery, bladder, esophagus and trachea. Isoform 1 is mostly found in slowly contracting tonic muscles. Mutations in MYH11 have been associated with acute myeloid leukemia and familial aortic aneurysm thoracic type 4.
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Species Reactivity:
Human
Mouse
Application Notes:
Western Blotting Analysis: A 1:500 dilution from a representative lot detected MYH11 in 10 µg of mouse colon tissue lysate.
Immunohistochemistry Analysis: A 1:50 dilution from a representative lot detected MYH11 in human prostate cancer and human Vans Deferens tissues.
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Control:
Human colon tissue lysate
Quality Assurance:
Evaluated by Western Blotting in human colon tissue lysate.
Western Blotting Analysis: A 1:500 dilution of this antibody detected MYH11 in 30 µg of human colon tissue lysate.
Purification Method:
Antigen Affinity purified
Presentation:
Purified rabbit polyclonal in buffer containing PBS with 0.1% sodium azide and 50% glycerol.
Storage Conditions:
Stable for 1 year at -20°C from date of receipt.
Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
Note: Variability in freezer temperatures below -20°C may cause glycerol containing solutions to become frozen during storage.
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UniProt Number:
P35749
Gene Symbol:
MYH11
Alternate Names:
Myosin heavy chain 11
Myosin heavy chain, smooth muscle isoform
SMMHC
Usage Statement:
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Key Applications:
Western Blotting
Immunohistochemistry
Product Name:
Anti-MYH11 Antibody
Concentration:
Please refer to the Certificate of Analysis for the lot-specific concentration.
Antibody Type:
Polyclonal Antibody
Qty/Pk:
150 µL
Format:
Affinity Purified
Host:
Rabbit
Applications:
Detect the MYH11 protein using this Anti-MYH11 validated for use in WB & IHC.

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