Screen Quest Fluorimetric MDR Assay Kit *1 Plate*
货号: 36340 产品名称: Screen Quest Fluorimetric MDR Assay Kit *1 Plate* 品牌: AAT 规格: 1 kit 二周到货 生化试验
Screen Quest� Fluorimetric MDR Assay Kit *1 Plate*
Cat # 36340
Storage F/D/L (see storage codes below)
Unit Size 100 Tests
Ex (nm) 492
Em (nm) 514
Tumor cell resistance to cytotoxic drugs is considered one of the major obstacles to successful chemotherapy. Some tumors are initially resistant and never respond to cytostatic drug treatment; others initially respond well but eventually regrow and become resistant. This phenomenon may result from genetic mutations induced by the administered antitumor agent, or may represent the selection of preexisting resistant cell populations in the malignant tumor. Multi-drug resistance (MDR) is a major factor in the failure of many forms of chemotherapy. In the past few years it has become widely accepted that the resistance to chemotherapy correlates with the overexpression of at least two ATP-dependent drug-efflux pumps. These cell membrane proteins, called P-glycoprotein (Pgp, MDR1), and multidrug-resistance-associated protein (MRP1) are members of the ABC transporter family. Our assay kit uses a fluorescent MDR indicator for assaying these two MDR pump activities. This hydrophobic fluorescent dye molecule rapidly penetrates cell membranes and becomes trapped in cells. Following a short incubation, the intracellular free dye concentration can increase significantly. In the MDR1 and/or MRP1-expressing cells this dye is extruded by the MDR transporter, thus decreasing the cellular fluorescence intensity. However, when its extrusion is blocked by an agent that interferes with the MDR1 and/or MRP1 pump-activity, its cellular fluorescence intensity increases significantly. Our MDR assay kit provides all the essential components with an optimized assay method. The assay can be performed in a convenient 96-well or 384-well microtiter-plate format and easily adapted to automation. This assay kit is ideal for high throughput screening of MDR pump inhibitors or identifying the cells that have high level of MDR pump activities.
Detailed Information: Protocol References MSDS Spectrum
Related Products: MDR Research Cell Metabolism Cell Functional Analysis
Storage Codes:
D = Desiccated
L = Avoid light
F = Freeze (<-15 癈)
R = Refrigerated (2-4 癈)
RT = Room temperature (4-50 癈)
![](http://thermofisher.jinpanbio.com/wp-content/uploads/2024/04/BZWZ.jpg)
36341 Screen Quest Fluorimetric MDR Assay Kit *10 Plates* 品牌 AAT
Screen Quest Fluorimetric MDR Assay Kit *10 Plates*
货号: 36341 产品名称: Screen Quest Fluorimetric MDR Assay Kit *10 Plates* 品牌: AAT 规格: 1 kit 二周到货 生化试验
Screen Quest� Fluorimetric MDR Assay Kit *10 Plates*
Cat # 36341
Storage F/D/L (see storage codes below)
Unit Size 1 kit
Ex (nm) 492
Em (nm) 514
Tumor cell resistance to cytotoxic drugs is considered one of the major obstacles to successful chemotherapy. Some tumors are initially resistant and never respond to cytostatic drug treatment; others initially respond well but eventually regrow and become resistant. This phenomenon may result from genetic mutations induced by the administered antitumor agent, or may represent the selection of preexisting resistant cell populations in the malignant tumor. Multi-drug resistance (MDR) is a major factor in the failure of many forms of chemotherapy. In the past few years it has become widely accepted that the resistance to chemotherapy correlates with the overexpression of at least two ATP-dependent drug-efflux pumps. These cell membrane proteins, called P-glycoprotein (Pgp, MDR1), and multidrug-resistance-associated protein (MRP1) are members of the ABC transporter family. Our assay kit uses a fluorescent MDR indicator for assaying these two MDR pump activities. This hydrophobic fluorescent dye molecule rapidly penetrates cell membranes and becomes trapped in cells. Following a short incubation, the intracellular free dye concentration can increase significantly. In the MDR1 and/or MRP1-expressing cells this dye is extruded by the MDR transporter, thus decreasing the cellular fluorescence intensity. However, when its extrusion is blocked by an agent that interferes with the MDR1 and/or MRP1 pump-activity, its cellular fluorescence intensity increases significantly. Our MDR assay kit provides all the essential components with an optimized assay method. The assay can be performed in a convenient 96-well or 384-well microtiter-plate format and easily adapted to automation. This assay kit is ideal for high throughput screening of MDR pump inhibitors or identifying the cells that have high level of MDR pump activities.
Detailed Information: Protocol References MSDS Spectrum
Related Products: MDR Research Cell Metabolism Cell Functional Analysis
Storage Codes:
D = Desiccated
L = Avoid light
F = Freeze (<-15 癈)
R = Refrigerated (2-4 癈)
RT = Room temperature (4-50 癈)
![](http://thermofisher.jinpanbio.com/wp-content/uploads/2024/04/BZWZ.jpg)
MA135605 MDR3 P-glycoprotein 品牌 Pierce
MDR3 P-glycoprotein
货号: MA135605 产品名称: MDR3 P-glycoprotein 品牌: Pierce 规格: 1 ML
![](http://thermofisher.jinpanbio.com/wp-content/uploads/2024/04/BZWZ.jpg)
4456753 MDR,PWR CORD SET C19 2.5M RC 品牌 Invitrogen
MDR,PWR CORD SET C19 2.5M RC
货号: 4456753 产品名称: MDR,PWR CORD SET C19 2.5M RC 品牌: Invitrogen 规格: ***
![](http://thermofisher.jinpanbio.com/wp-content/uploads/2024/04/BZWZ.jpg)
4465598 MDR,UPS 30A HARDWIRD POWR CORD 品牌 Invitrogen
MDR,UPS 30A HARDWIRD POWR CORD
货号: 4465598 产品名称: MDR,UPS 30A HARDWIRD POWR CORD 品牌: Invitrogen 规格: ***
![](http://thermofisher.jinpanbio.com/wp-content/uploads/2024/04/BZWZ.jpg)
4465627 MDR, CHILLER FOR COVARIS-SOLID 品牌 Invitrogen
MDR, CHILLER FOR COVARIS-SOLID
货号: 4465627 产品名称: MDR, CHILLER FOR COVARIS-SOLID 品牌: Invitrogen 规格: ***
![](http://thermofisher.jinpanbio.com/wp-content/uploads/2024/04/BZWZ.jpg)
4474643 MDR, MAILER, CHIP SET RETURN 品牌 Invitrogen
MDR, MAILER, CHIP SET RETURN
货号: 4474643 产品名称: MDR, MAILER, CHIP SET RETURN 品牌: Invitrogen 规格: ***
![](http://thermofisher.jinpanbio.com/wp-content/uploads/2024/04/BZWZ.jpg)
4476879 MDR,4200W UPS 品牌 Invitrogen
MDR,4200W UPS
货号: 4476879 产品名称: MDR,4200W UPS 品牌: Invitrogen 规格: ***
![](http://thermofisher.jinpanbio.com/wp-content/uploads/2024/04/BZWZ.jpg)
MDR1734 Zebrafish full length clone 品牌 Open Bio
Zebrafish full length clone
货号: MDR1734 产品名称: Zebrafish full length clone 品牌: Open Bio 规格: EA
![](http://thermofisher.jinpanbio.com/wp-content/uploads/2024/04/BZWZ.jpg)
MDR1738 IMCB Zebrafish Clone 品牌 Open Bio
IMCB Zebrafish Clone
货号: MDR1738 产品名称: IMCB Zebrafish Clone 品牌: Open Bio 规格: EA
![](http://thermofisher.jinpanbio.com/wp-content/uploads/2024/04/BZWZ.jpg)
MDR1739 IMCB Zebrafish Library 品牌 Open Bio
IMCB Zebrafish Library
货号: MDR1739 产品名称: IMCB Zebrafish Library 品牌: Open Bio 规格: EA
![](http://thermofisher.jinpanbio.com/wp-content/uploads/2024/04/BZWZ.jpg)
MDR4775 Zebrafish MGC verified FL cDNA (IRQL) 品牌 Open Bio
Zebrafish MGC verified FL cDNA (IRQL)
货号: MDR4775 产品名称: Zebrafish MGC verified FL cDNA (IRQL) 品牌: Open Bio 规格: EA
![](http://thermofisher.jinpanbio.com/wp-content/uploads/2024/04/BZWZ.jpg)
MDR4778 Zebrafish MGC verified FL cDNA (IRQM) 品牌 Open Bio
Zebrafish MGC verified FL cDNA (IRQM)
货号: MDR4778 产品名称: Zebrafish MGC verified FL cDNA (IRQM) 品牌: Open Bio 规格: EA
![](http://thermofisher.jinpanbio.com/wp-content/uploads/2024/04/BZWZ.jpg)
ALX-801-028-C125 MDR3 P-glycoprotein (human), mAb (P3II-26) 品牌 Alexis
MDR3 P-glycoprotein (human), mAb (P3II-26)
货号: ALX-801-028-C125 产品名称: MDR3 P-glycoprotein (human), mAb (P3II-26) 品牌: Alexis 规格: 125.micro.g 3周到货 生化实验
ALX-801-028-C250 MDR3 P-glycoprotein (human), mAb (P3II-26) 品牌 Alexis
MDR3 P-glycoprotein (human), mAb (P3II-26)
货号: ALX-801-028-C250 产品名称: MDR3 P-glycoprotein (human), mAb (P3II-26) 品牌: Alexis 规格: 250.micro.g 3周到货 生化实验