KLENOW FRAGEL DNA FRAGMENT. DETECT. KIT

货号： QIA21-1EA      产品名称： KLENOW FRAGEL DNA FRAGMENT. DETECT. KIT   品牌： Merck 规格： *** 3周到货 生化实验

FragEL™ DNA Fragmentation Detection Kit, Colorimetric – Klenow Enzyme
Non-isotopic kit for the detection of apoptosis at the individual cell level. Apoptotic morphology is easily detected. This kit allows the recognition of apoptotic nuclei in paraffin-embedded tissue sections, tissue cryosections, or in cell preparations fixed on slide by Fragment End Labeling (FragEL™) of DNA. Klenow binds to exposed ends of DNA fragments generated in response to apoptotic signals and catalyzes the template-dependent addition of biotin-labeled and unlabeled deoxynucleotides. Biotinylated nucleotides are detected using streptavidin-horseradish peroxidase (HRP) conjugate. Diaminobenzidine reacts with the labeled sample to generate an insoluble colored product at the site of DNA fragmentation. Counterstaining with methyl green aids in the morphological evaluation and characterization of normal and apoptotic cells.
产品信息
Format  Microscopy
Form  50 Tests
Detection method  Colorimetric
Species reactivity  a broad range of species
Applications 
Frozen Sections
Paraffin Sections
Avoid freeze/thaw  是的
Assay time  4 h
Sample type  Frozen or paraffin sections or fixed-cell preparations
Kit contains  Counterstain, Proteinase K, Equilibration and Labeling Buffers, Klenow Enzyme (Cat. No. PF059), Stop buffer, Blocking Buffer, 50X Conjugate, DAB, Positive and Negative Control Slides, H2O2/Urea Tablets, Methyl Green Counterstain, and a user protocol.
商店和运输信息
Hazardous Materials Attention: Due to the nature of the Hazardous Materials in this shipment, additional shipping chargesmay be applied to your order. Certain sizes may be exempt from the additional hazardous materials shipping charges. Please contact your local sales office for more information regarding these charges.
Storage  -20°C
Ship  Dry Ice Only
Multiple Toxicity Values, refer to MSDS

-20°C