标签： kdr

V2681   KDR Kinase Enzyme System   品牌 Promega

KDR Kinase Enzyme System

货号： V2681      产品名称： KDR Kinase Enzyme System   品牌： Promega 规格： 10μg 二周到货 生化试验

说明  
 在 Sf9 昆虫细胞中，我们通过杆状病毒表达 N 端带 GST 标签的重组人源 KDR （第 789 位 氨基酸 – 末端 ） 。 KDR （激酶插入结构域受体）是一个生长因子受体酪氨酸激酶，最初从人内皮细胞中分离得到，在内皮细胞的增殖和分化过程中起着至关重要的作用。 KDR 及其在小鼠中的同源蛋白 Flk1 ，都能与 VEGF 紧密结合，参与新血管的生成过程。在人类眼部血管的正常发育过程中， VEGF 与 KDR 的表达水平是密切关联的。血管生成的诱导是肿瘤发生的关键步骤，在临床前模型中， KDR 抑制剂既能诱导肿瘤衰退，又能降低肿瘤转移的可能。
ADP-Glo™ Kinase Assay （ADP-Glo™ 激酶检测试剂盒） 是一种发光法激酶检测试剂盒，用于 检测 激酶反应产生的 ADP 含量： ADP 转化为 ATP 后， ATP 即可作为 Ultra-Glo™ 萤光素酶 催化反应的底物，产生光信号。发光信号与 ADP 的量和激酶活性正相关。 该试剂盒适合检测化合物对纯化的多种激酶活性的影响，使之成为初筛和激酶选择性概况分析的理想工具。 ADP-Glo™ 激酶检测试剂盒可用于检测几乎所有可生成 ADP 的酶类 ( 如激酶或 ATP 酶 ) 的活性， ATP 浓度可高达 1 mM 。
激酶酶系统的组成：
•  激酶： KDR ， 10μg ( 人源，重组， 第789 位 氨基酸 – 末端 ) ； MW : ~110kDa 。
•  底物： Poly (4:1 Glu, Tyr) Peptide 。
•  其它组分： 反应缓冲液， DTT 。
KDR NCBI 数据库入口。
人类激酶酶系统及其应用。 
特点  •  在实验室内即可筛选更多的化合物： ADP-Glo™ 激酶检测试剂盒 与激酶酶系统的优化组合，使您可以迅速方便地开始实验。
•  完整的系统： 激酶酶系统包含重组激酶、激酶的最适底物、反应缓冲液、以及使用前需加入缓冲液的 DTT 和 MnCl2。
•  可得到可靠结果： ADP-Glo™ 激酶检测试剂盒提供了宽广的动态范围、方便的操作方法、以及更好的灵敏度，产生的数据更可靠。

 
 

BML-P240-0001   KDR/VEGFR2 Peptide Substrate   品牌 Biomol

KDR/VEGFR2 Peptide Substrate

货号： BML-P240-0001      产品名称： KDR/VEGFR2 Peptide Substrate   品牌： Biomol 规格： 1 mg 3周到货 生化实验

Product Specification
Alternative Name:KDR substrate
Sequence:Glu-Glu-Glu-Glu-Ala-Tyr-Gly-Trp-Leu-Asp-Phe
MW:1387.4
Purity:95%
Long Term Storage:-20°C

-20°C

BML-P241-0001   KDR/VEGFR Peptide Substrate (Biotinylated)   品牌 Biomol

KDR/VEGFR Peptide Substrate (Biotinylated)

货号： BML-P241-0001      产品名称： KDR/VEGFR Peptide Substrate (Biotinylated)   品牌： Biomol 规格： 1 mg 3周到货 生化实验

Product Specification
Alternative Name:KDR substrate
Sequence:Glu-Glu-Glu-Glu-Ala-Tyr-Gly-Trp-Leu-Asp-Phe
MW:1387.4
Purity:95%
Long Term Storage:-20°C

-20°C

04-894   a-p-KDR/Flk-1/VEGFR2(Y1054) 100ul   品牌 Millipore

a-p-KDR/Flk-1/VEGFR2(Y1054) 100ul

货号： 04-894      产品名称： a-p-KDR/Flk-1/VEGFR2(Y1054) 100ul   品牌： Millipore 规格： EA 3周到货 生化实验

Anti-phospho-KDR/Flk-1/VEGFR2 (Tyr1054) Antibody, clone D1W, rabbit monoclonal
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PRODUCT FAMILY INFORMATION
Endothelial Cell Markers
The thin layer of cells that lines the interior surface of blood vessels and lymphatic vessels, forming an interface between circulating blood or lymph in the lumen and the rest of the vessel wall.
Endothelial cells are the thin layer of cells that lines the interior surface of blood vessels and lymphatic vessels, forming an interface between circulating blood or lymph in the lumen and the rest of the vessel wall. Endothelial cells in direct contact with blood are called vascular endothelial cells, whereas those in direct contact with lymph are known as lymphatic endothelial cells.
» More…
Species Reactivity Key Applications Host Format Antibody Type
H, M  WB Rabbit Culture Supernatant Monoclonal Antibody
Description:
Anti-phospho-KDR/Flk-1/VEGFR2 (Tyr1054) Antibody, clone D1W, rabbit monoclonal
Promotional Text:
Special Shipping Offer on Antibodies
100% Performance Guaranteed
Trade Name:
Upstate (Millipore)
Specificity:
KDR/Flk-1/VEGFR2 phosphorylated on Tyrosine 1054.
Molecular Weight:
152 kDa.
Immunogen:
KLH-conjugated, synthetic peptide encompassing the phosphorylated tyrosine residue 1054 of human KDR/Flk-1/VEGFR2.
Modifications:
Phosphorylation
Clone:
D1W
Isotype:
IgG
Species Reactivity:
Human
Mouse
Species Reactivity Note:
Predicted to have broad species cross-reactivity based on sequence homology.
Application Notes:
Western Blot: 1:1000-1:4000 dilution for the detection of phospho-KDR/Flk-1/VEGFR2 in RIPA lysates from CSF stimulated porcine aortic endothelial (PAE) cells expressing a human CSF-R1/murine KDR chimeric receptor.
Quality Assurance:
Routinely evaluated by immunoblot in RIPA lysates from CSF stimulated porcine aortic endothelial (PAE) cells expressing a human CSF-R1/murine KDR chimeric receptor.
Presentation:
Cultured supernantant with 0.05% sodium azide
Storage Conditions:
2 years at -20°C from date of shipment.
UniProt Number:
P35968
Entrez Gene Number:
NP_002244.1
Gene Symbol:
KDR
VEGFR-2
VEGFR2
VEGFR
CD309
FLK1
View All »
Usage Statement:
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Key Applications:
Western Blotting
UniProt Summary:
FUNCTION: SwissProt: P35968 # Receptor for VEGF or VEGFC. Has a tyrosine-protein kinase activity. The VEGF-kinase ligand/receptor signaling system plays a key role in vascular development and regulation of vascular permeability. In case of HIV-1 infection, the interaction with extracellular viral Tat protein seems to enhance angiogenesis in Kaposi’s sarcoma lesions.
SIZE: 1356 amino acids; 151527 Da
SUBUNIT: Interacts with SHB upon VEGF activation. Interacts with HIV-1 Tat.
SUBCELLULAR LOCATION: Membrane; Single-pass type I membrane protein.
SIMILARITY: SwissProt: P35968 ## Belongs to the protein kinase superfamily. Tyr protein kinase family. CSF-1/PDGF receptor subfamily. & Contains 7 Ig-like C2-type (immunoglobulin-like) domains. & Contains 1 protein kinase domain.
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Brand Family:
Upstate
Product Name:
Anti-phospho-KDR/Flk-1/VEGFR2 -20°C

04-894-K   a-p-KDR/Flk-1/VEGFR2(Y1054) – ML   品牌 Millipore

a-p-KDR/Flk-1/VEGFR2(Y1054) – ML

货号： 04-894-K      产品名称： a-p-KDR/Flk-1/VEGFR2(Y1054) – ML   品牌： Millipore 规格： ML 三周到货 生化实验

Anti-phospho-KDR/Flk-1/VEGFR2 (Tyr1054) Antibody, clone D1W, rabbit monoclonal
Close
PRODUCT FAMILY INFORMATION
Endothelial Cell Markers
The thin layer of cells that lines the interior surface of blood vessels and lymphatic vessels, forming an interface between circulating blood or lymph in the lumen and the rest of the vessel wall.
Endothelial cells are the thin layer of cells that lines the interior surface of blood vessels and lymphatic vessels, forming an interface between circulating blood or lymph in the lumen and the rest of the vessel wall. Endothelial cells in direct contact with blood are called vascular endothelial cells, whereas those in direct contact with lymph are known as lymphatic endothelial cells.
» More…
Species Reactivity Key Applications Host Format Antibody Type
H, M  WB Rabbit Culture Supernatant Monoclonal Antibody
Description:
Anti-phospho-KDR/Flk-1/VEGFR2 (Tyr1054) Antibody, clone D1W, rabbit monoclonal
Promotional Text:
Special Shipping Offer on Antibodies
100% Performance Guaranteed
Trade Name:
Upstate (Millipore)
Specificity:
KDR/Flk-1/VEGFR2 phosphorylated on Tyrosine 1054.
Molecular Weight:
152 kDa.
Immunogen:
KLH-conjugated, synthetic peptide encompassing the phosphorylated tyrosine residue 1054 of human KDR/Flk-1/VEGFR2.
Modifications:
Phosphorylation
Clone:
D1W
Isotype:
IgG
Species Reactivity:
Human
Mouse
Species Reactivity Note:
Predicted to have broad species cross-reactivity based on sequence homology.
Application Notes:
Western Blot: 1:1000-1:4000 dilution for the detection of phospho-KDR/Flk-1/VEGFR2 in RIPA lysates from CSF stimulated porcine aortic endothelial (PAE) cells expressing a human CSF-R1/murine KDR chimeric receptor.
Quality Assurance:
Routinely evaluated by immunoblot in RIPA lysates from CSF stimulated porcine aortic endothelial (PAE) cells expressing a human CSF-R1/murine KDR chimeric receptor.
Presentation:
Cultured supernantant with 0.05% sodium azide
Storage Conditions:
2 years at -20°C from date of shipment.
UniProt Number:
P35968
Entrez Gene Number:
NP_002244.1
Gene Symbol:
KDR
VEGFR-2
VEGFR2
VEGFR
CD309
FLK1
View All »
Usage Statement:
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
View All »
Key Applications:
Western Blotting
UniProt Summary:
FUNCTION: SwissProt: P35968 # Receptor for VEGF or VEGFC. Has a tyrosine-protein kinase activity. The VEGF-kinase ligand/receptor signaling system plays a key role in vascular development and regulation of vascular permeability. In case of HIV-1 infection, the interaction with extracellular viral Tat protein seems to enhance angiogenesis in Kaposi’s sarcoma lesions.
SIZE: 1356 amino acids; 151527 Da
SUBUNIT: Interacts with SHB upon VEGF activation. Interacts with HIV-1 Tat.
SUBCELLULAR LOCATION: Membrane; Single-pass type I membrane protein.
SIMILARITY: SwissProt: P35968 ## Belongs to the protein kinase superfamily. Tyr protein kinase family. CSF-1/PDGF receptor subfamily. & Contains 7 Ig-like C2-type (immunoglobulin-like) domains. & Contains 1 protein kinase domain.
View All »
Brand Family:
Upstate
Product Name:
Anti-phospho-KDR/Flk-1/VEGFR2 -20℃

05-554   ANTI-KDR/FLK-1, CLONE CH-11   品牌 Millipore

ANTI-KDR/FLK-1, CLONE CH-11

货号： 05-554      产品名称： ANTI-KDR/FLK-1, CLONE CH-11   品牌： Millipore 规格： EA 3周到货 生化实验

Anti-KDR/Flk-1/VEGFR2 Antibody, clone CH-11
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REFERENCES
Rapid transactivation of the vascular endothelial growth factor receptor KDR/Flk-1 by the bradykinin B2 receptor contributes to endothelial nitric-oxide synthase activation in cardiac capillary endothelial cells.
Thuringer, Dominique, et al. (2002) J. Biol. Chem., 277: 2028-32 (2002)
Species Reactivity Key Applications Host Format Antibody Type
H, M  ELISA, ICC, WB Mouse Purified Monoclonal Antibody
Description:
Anti-KDR/Flk-1/VEGFR2 Antibody, clone CH-11
Promotional Text:
Special Shipping Offer on Antibodies
100% Performance Guaranteed
Trade Name:
Upstate (Millipore)
Specificity:
KDR/Flk-1
Molecular Weight:
230kDa
Immunogen:
Recombinant protein corresponding to residues 1158-1345 of murine KDR/Flk-1
Isotype:
IgG1
Species Reactivity:
Human
Mouse
Quality Assurance:
routinely evaluated by immunoblot on RIPA lysates from HUVEC cells
Purification Method:
Protein A chromatography
Presentation:
20mM sodium phosphate, pH 7.6, 250mM NaCl with 0.1% sodium azide
Storage Conditions:
2 years at 4°C
UniProt Number:
P35968
Entrez Gene Number:
NM_010612
Gene Symbol:
KDR
VEGFR-2
VEGFR2
VEGFR
CD309
FLK1
View All »
Usage Statement:
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
View All »
Key Applications:
ELISA
Immunocytochemistry
Western Blotting
UniProt Summary:
FUNCTION: SwissProt: P35968 # Receptor for VEGF or VEGFC. Has a tyrosine-protein kinase activity. The VEGF-kinase ligand/receptor signaling system plays a key role in vascular development and regulation of vascular permeability. In case of HIV-1 infection, the interaction with extracellular viral Tat protein seems to enhance angiogenesis in Kaposi’s sarcoma lesions.
SIZE: 1356 amino acids; 151527 Da
SUBUNIT: Interacts with SHB upon VEGF activation. Interacts with HIV-1 Tat.
SUBCELLULAR LOCATION: Membrane; Single-pass type I membrane protein.
SIMILARITY: SwissProt: P35968 ## Belongs to the protein kinase superfamily. Tyr protein kinase family. CSF-1/PDGF receptor subfamily. & Contains 7 Ig-like C2-type (immunoglobulin-like) domains. & Contains 1 protein kinase domain.
View All »
Brand Family:
Upstate
Product Name:
Anti-KDR/Flk-1/VEGFR2 Antibody, clone CH-11
Antibody Type:
Monoclonal Antibody
Qty/Pk:
100 µg
Format:
Purified
Host:
Mouse
Applications:
Anti-KDR/Flk-1/VEGFR2 Antibody, clone CH-11 detects level of KDR/Flk-1/VEGFR2 & has been published & validated for use in ELISA, IC & WB.

+4°C

07-1294   Anti-phospho-KDR/Flk-1/VEGFR2 (Y1223)   品牌 Millipore

Anti-phospho-KDR/Flk-1/VEGFR2 (Y1223)

货号： 07-1294      产品名称： Anti-phospho-KDR/Flk-1/VEGFR2 (Y1223)   品牌： Millipore 规格： EA 3周到货 生化实验

Anti-phospho-VEGFR2/Flk-1/KDR (Tyr1223) Antibody
Close
PRODUCT FAMILY INFORMATION
Endothelial Cell Markers
The thin layer of cells that lines the interior surface of blood vessels and lymphatic vessels, forming an interface between circulating blood or lymph in the lumen and the rest of the vessel wall.
Endothelial cells are the thin layer of cells that lines the interior surface of blood vessels and lymphatic vessels, forming an interface between circulating blood or lymph in the lumen and the rest of the vessel wall. Endothelial cells in direct contact with blood are called vascular endothelial cells, whereas those in direct contact with lymph are known as lymphatic endothelial cells.
» More…
Species Reactivity Key Applications Host Format Antibody Type
M, R, Pm, Ca, B, Po, Eq  WB Rabbit Affinity Purified Polyclonal Antibody
Description:
Anti-phospho-VEGFR2/Flk-1/KDR (Tyr1223) Antibody
Promotional Text:
Special Shipping Offer on Antibodies
100% Performance Guaranteed
Trade Name:
Upstate (Millipore)
Specificity:
Recognizes VEGFR2/Flk-1/KDR when phosphorylated on Tyrosine 1223 (human).
Molecular Weight:
152 kDa
Immunogen:
Synthetic peptide encompassing phosphorylated Tyr1223 of human VEGFR2.
Modifications:
Phosphorylation
Background Information:
The Flt gene family of receptor tyrosine kinases includes Flt-1, KDR/Flk-1, Flt-3, and Flt-4. They encode receptors for VEGF, characterized by seven Ig-loops within their extracellular domain and a split kinase domain within the cytoplasmic domain. VEGF is an important angiogenic factor of many solid tumors and is also involved in the differentiation of endothelial cells. Flt-1 (Fms-like tyrosine kinase, VEGF-R1) is expressed on endothelial cells and on the common precursors of endothelial and hematopoietic stem cells. KDR (Flk-1, VEGF-R2) is the principal receptor involved in VEGF signaling. KDR is expressed on endothelial cells and stimulates angiogenesis, but is also expressed on bone marrow cells, and is thought to play a role in driving the proliferation of most or all leukemic cells. Flt3 is a class III receptor tyrosine kinase that is closely related to c-Kit and c-Fms. It is expressed in primitive hematopoietic cells and its ligand exerts proliferative effects on these cells in co-operation with other cytokines. The VEGF receptors stimulate the Ras-MAP kinase pathway, suggesting that it signals as a conventional receptor tyrosine kinase.
View All »
Species Reactivity:
Mouse
Rat
Primate
Canine
Bovine
Pig
Horse
View All »
Species Reactivity Note:
Human tested. Expected to react with Mouse, Rat, Primate, Canine, Cow, Pig, Horse, based on sequence homolgy.
Control:
CSF-1 treated PAE cell lysate, transfected with CSF/KDR.
Quality Assurance:
Western Blot Analysis:
This lot detected VEGFR2 phosphorylated on Tyr1223 at a 1:1,000 dilution in PAE cells transfected with CSF/KDR and treated with 40ng/mL CSF-1 for 15 min. Cell lysate resolved via SDS-PAGE and transferred to PVDF (Immobilon-P).
Purification Method:
Dual Antigen Affinity purification
Presentation:
Double affinity purified rabbit polyclonal in PBS with 0.05% NaN3 and 50% glycerol.
Storage Conditions:
Stable for 1 year at -20°C from date of receipt.
Handling Recommendations: Upon receipt, and prior to removing the cap, centrifuge the vial and gently mix the solution.
UniProt Number:
P35968
Entrez Gene Number:
NM_002253.2
Gene Symbol:
KDR
VEGFR-2
VEGFR2
VEGFR
CD309
FLK1
EC 2.7.10.1
View All »
Alternate Names:
Ki -20°C

07-158   ANTI-KDR/VEGFR2 200UL   品牌 Millipore

ANTI-KDR/VEGFR2 200UL

货号： 07-158      产品名称： ANTI-KDR/VEGFR2 200UL   品牌： Millipore 规格： EA 三周到货 生化实验

Anti-KDR/Flk-1/VEGFR2 Antibody
Species Reactivity Key Applications Host Format Antibody Type
M  WB, IHC Rabbit Affinity Purified Polyclonal Antibody
Description:
Anti-KDR/Flk-1/VEGFR2 Antibody
Promotional Text:
Special Shipping Offer on Antibodies
100% Performance Guaranteed
Trade Name:
Upstate (Millipore)
Specificity:
This antibody recognizes KDR/Flk-1/VEGFR2 at the cytoplasmic domain.
Molecular Weight:
~150/180 kDa observed. 180 kDa is the mature form and 150 kDa is the immature unmodified form. In human and mouse cells the mature form of VEGFR2 is ~220-225 kDa. Faint band at ~225 kDa may be endogenous VEGFR2 in PAE cells.
Epitope:
Cytoplasmic domain
Immunogen:
GST-tagged recombinant protein corresponding to mouse KDR/Flk-1/VEGFR2 at the cytoplasmic domain.
Background Information:
The Flt gene family of receptor tyrosine kinases includes Flt-1, KDR/Flk-1, Flt-3, and Flt-4. They encode receptors for VEGF, characterized by seven Ig-loops within their extracellular domain and a split kinase domain within the cytoplasmic domain. VEGF is an important angiogenic factor of many solid tumors and is also involved in the differentiation of endothelial cells. Flt-1 (Fms-like tyrosine kinase, VEGF-R1) is expressed on endothelial cells and on the common precursors of endothelial and hematopoietic stem cells. KDR (Flk-1, VEGF-R2) is the principal receptor involved in VEGF signaling. KDR is expressed on endothelial cells and stimulates angiogenesis, but is also expressed on bone marrow cells, and is thought to play a role in driving the proliferation of most or all leukemic cells. Flt3 is a class III receptor tyrosine kinase that is closely related to c-Kit and c-Fms. It is expressed in primitive hematopoietic cells and its ligand exerts proliferative effects on these cells in co-operation with other cytokines. The VEGF receptors stimulate the Ras-MAP kinase pathway, suggesting that it signals as a conventional receptor tyrosine kinase.
Species Reactivity:
Mouse
Species Reactivity Note:
Demonstrated to react with Mouse. Expected to react with Rat based on a 99% sequence homology and Human based on 92% sequence homology.
Application Notes:
Immunohistochemistry Analysis: 1:100 dilution from a representative lot detected KDR/Flk-1/VEGFR2 in mouse embryo vascular endothelium tissue.
Control:
PAE cells transfected with CSF/VEGFR2 chimera and treated with CSF
Quality Assurance:
Evaluated by Western Blot in PAE cells transfected with CSF/VEGFR2 chimera and treated with CSF.
Western Blot Analysis: 1:1,000 dilution of this antibody detected KDR/Flk-1/VEGFR2 on 10 µg of PAE cells transfected with CSF/VEGFR2 chimera and treated with CSF.
Purification Method:
Affinity purified
Presentation:
Purified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Storage Conditions:
Stable for 1 year at 2-8°C from date of receipt.
UniProt Number:
P35968
Entrez Gene Number:
NP_002244
Gene Symbol:
KDR
VEGFR-2
VEGFR2
FLK1
VEGFR
CD309
Alternate Names:
Vascular endothelial growth factor receptor 2
VEGFR-2
Fetal liver kinase 1
FLK-1
Kinase insert domain receptor
Protein-tyrosine kinase receptor flk-1
CD309
Usage Statement:
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or appli 2-8℃