标签： ifng

hkb-ifng   HEK-Blue IFN-γ cells   品牌 Invivogen

HEK-Blue IFN-γ cells

货号： hkb-ifng      产品名称： HEK-Blue IFN-γ cells   品牌： Invivogen 规格： 3-7 x 10e6 二周到货 生化试验

HEK-Blue™ IFN-γ cells
HEK-Blue™ IFN-γ cells allow the detection of bioactive human IFN-γ by monitoring the activation of the JAK/STAT-1 pathway.
HEK-Blue™ IFN-γ cells produce SEAP in response to IFN-γ stimulation only. They are unresponsive to type I IFNs.
Levels of SEAP in the supernatant can be easily determined with QUANTI-Blue™.
Antibiotic resistance: blasticidin, Zeocin™
Growth medium: DMEM medium, 2mM L-glutamine,10% FBS supplemented with 100 µg/ml Normocin™, 30 µg/ml blasticidin and 100 µg/ml Zeocin™
Guaranteed mycoplasma-free
Shipped on dry ice
• Detection range for human IFN-γ: 5 – 100 IU/ml

 

hkd-ifng   HEK-Dual IFN-γ cells   品牌 Invivogen

HEK-Dual IFN-γ cells

货号： hkd-ifng      产品名称： HEK-Dual IFN-γ cells   品牌： Invivogen 规格： 3-7 x 10e6 二周到货 生化试验

HEK-Dual™ IFN-γ cells
HEK-Dual™ IFN-γ cells allow the detection of bioactive human IFN-γ by monitoring the activation of the JAK/STAT-1 pathway.
HEK-Dual™ IFN-γ cells were generated by stable transfection of HEK293 cells with the human STAT1 gene to obtain a fully active STAT1 pathway. The cells were further transfected with two reporter genes under the control of an IFN-γ inducible promoter.
As a result, HEK-Dual™ IFN-γ cells allow to study the activation of JAK/STAT-1 pathway, by monitoring the activity of two different reporters, SEAP or a secreted luciferase (Lucia).
Both reporter proteins are readily measurable in the cell culture supernatant when using QUANTI-Blue™, a SEAP detection reagent, and QUANTI-Luc™, a luciferase detection reagent.
Antibiotic resistance: blasticidin, Zeocin™, puromycin
Growth medium: DMEM medium, 2mM L-glutamine,10% FBS supplemented with 100 µg/ml Normocin™, 30 µg/ml blasticidin, 100 µg/ml Zeocin™ and 1 µg/ml puromycin
Guaranteed mycoplasma-free
Shipped on dry ice
• Detection range for human IFN-γ using QUANTI-Luc™: 1 – 10e3IU/ml
• Detection range for human IFN-γ using QUANTI-Blue™: 5 – 100IU/ml
These products are covered by a Limited Use License (See Terms and Conditions).