标签归档：hygro

pmod2-hygro   pMOD2-Hygro   品牌 Invivogen

发表于由

pMOD2-Hygro

货号： pmod2-hygro      产品名称： pMOD2-Hygro   品牌： Invivogen 规格： 20 μg 三周到货 生化试验

CpG-free hph
Synthetic Hygromycin resistance gene in cloning plasmid
Resistance to the antibiotic hygromycin is conferred by the hph gene encoding a hygromycin B phosphotransferase isolated from an E. coli strain. The DNA sequence of this CpG-free gene was modified by optimizing the codon usage and deleting the CpG motifs without changing the amino acid sequence of the wild type protein.
The synthetic hph gene is provided in the bacterial plasmid pMOD2 which is selectable with Ampicillin. To facilitate the excision and subcloning of the synthetic hph gene into another vector, this gene is flanked by a unique restriction site at the 5’ and 3’ end.
This gene is available in pMOD2 expression plasmid selectable with Ampicillin.
Bacteria Synthetic hph (pMOD2-Hygro)

pmonoh-gfp   pMONO-hygro-GFP   品牌 Invivogen

发表于由

pMONO-hygro-GFP

货号： pmonoh-gfp      产品名称： pMONO-hygro-GFP   品牌： Invivogen 规格： 20 μg 二周到货 生化试验

pMONO-hygro
 pMONO-hygro cloning plasmids are specifically designed for strong and constitutive expression of a gene of interest in a wide variety of cell lines. They allow the selection of stable transfectants with a hygromycin selectable marker. pMONO-hygro plasmids carry either a multiple cloning site (MCS) or a green fluorescent protein (GFP) reporter gene downstream of the promoter.
– Strong and Ubiquitous Expression of the Transgene
– Convenient cloning of a gene of interest whith a Multiple Cloning Site
– Selectable with hygromycin B in mammalian cells and E.coli

pmonoh-mcs   pMONO-hygro-mcs   品牌 Invivogen

发表于由

pMONO-hygro-mcs

货号： pmonoh-mcs      产品名称： pMONO-hygro-mcs   品牌： Invivogen 规格： 20 μg 二周到货 生化试验

pMONO-hygro
pMONO-hygro cloning plasmids are specifically designed for strong and constitutive expression of a gene of interest in a wide variety of cell lines. They allow the selection of stable transfectants with a hygromycin selectable marker. pMONO-hygro plasmids carry either a multiple cloning site (MCS) or a green fluorescent protein (GFP) reporter gene downstream of the promoter.
– Strong and Ubiquitous Expression of the Transgene
– Convenient cloning of a gene of interest whith a Multiple Cloning Site
– Selectable with hygromycin B in mammalian cells and E.coli

E1310   pGL4.50[luc2/CMV/Hygro] Vector   品牌 Promega

发表于由

pGL4.50[luc2/CMV/Hygro] Vector

货号： E1310      产品名称： pGL4.50[luc2/CMV/Hygro] Vector   品牌： Promega 规格： 20μg 二周到货 生化试验

说明  
 由启动子调控的Renilla 对照载体通常与实验用的萤火虫萤光素酶载体共转染，在Dual-Luciferase® 或Dual-Glo® 报告基因检测系统中使用。一般作为对照的Renilla 载体所表现出的活性水平是几乎不变的，而实验用的萤火虫萤光素酶载体因为不同的处理则变化很大。当实验用载体设计在Renilla 载体中时，可选择启动子调控的pGL4.13 萤火虫载体。pGL4.50 和pGL4.51 可用于标记细胞系，为建立稳定转染的细胞系提供选择Marker。pGL4.50 和pGL4.51 载体可标记细胞系，进行活体的生物发光成像的应用。    
特点    基于以下原因拥有更好的灵敏度和生物相关性 ：
• 报告基因表达更高 ：为了更适于哺乳动物细胞的表达，将合成的基因密码子更加优化。
• 降低了表达产物的背景和危险 ：去掉了隐藏的DNA 调控元件和转录因子结合位点。
• 瞬时应答更快 ：Rapid Response ™ 技术应用了去稳定萤光素酶基因。
其它的优势包括：
• 更灵活的检测选择 ：可随意选择合成luc2 (Photinus pyralis ) 或hRluc (Renilla reniformis ) 报告基。
• 可轻松地从瞬时转换到稳定细胞 ：有多种哺乳动物细胞选择标记可供选择。
• 可轻松在载体间转移 ：具有常用的多克隆位点和独特的SfiI 转移设计。

 

E1340   pGL4.33[luc2p/SRE/Hygro]Vector   品牌 Promega

发表于由

pGL4.33[luc2p/SRE/Hygro]Vector

货号： E1340      产品名称： pGL4.33[luc2p/SRE/Hygro]Vector   品牌： Promega 规格： 20μg 二周到货 生化试验

说明 
 构建一个可以检测某一功能信号通路的细胞系，可以有助于解译参与这一信号通路的信号分子。这些工具载体是通过在载体的最小启动子的上游插入多个反应元件重复序列而获得的。我们提供含有三种经过改造的不同萤火虫萤光素酶基因(luc2 、luc2P 或luc2CP)的最小启动子载体。luc2 基因经过改造，去除了大部分隐藏的转录因子结合位点，并通过编码优化提高了在哺乳动物细胞中的表达效率。luc2P、luc2CP 和RapidResponse™  基因是在luc2 基因的基础上增加了降解序列，从而可以调控luc2 基因在细胞中的半衰期。RapidResponse™  基因对刺激的响应更快，但是以牺牲信号强度为代价。luc2P 基因( 半衰期为1 个小时) 反应比luc2 基因( 半衰期为3 个小时) 快，信号强度也适合，而luc2CP 基因( 半衰期为0.4个小时) 反应更快但信号强度最弱。最小启动子载体有含选择标签(hygromycin，潮霉素) 和不含选择标签的可供订购。为了提高实验效率，一些预设计的反应元件载体已经整合到了pGL4.27 载体中。其中一些载体也可订购其稳定表达的细胞系。 
特点    • 基于以下原因拥有更好的灵敏度和生物相关性：
• 报告基因表达更高：为了更适于哺乳动物细胞的表达，将合成的基因密码子更加优化。
• 降低了表达产物的背景和危险：去掉了隐藏的DNA 调控元件和转录因子结合位点。
• 瞬时应答更快：Rapid Response™ 技术应用了去稳定萤光素酶基因。
其它的优势包括：
• 更灵活的检测选择：可随意选择合成luc2 (Photinus pyralis ) 或hRluc (Renilla reniformis ) 报告基因。
• 可轻松地从瞬时转换到稳定细胞：有多种哺乳动物细胞选择标记可供选择。
• 可轻松在载体间转移：具有常用的多克隆位点和独特的SfiI 转移设计。

 

E1360   pGL4.36[luc2P/MMTV/Hygro]Vector   品牌 Promega

发表于由

pGL4.36[luc2P/MMTV/Hygro]Vector

货号： E1360      产品名称： pGL4.36[luc2P/MMTV/Hygro]Vector   品牌： Promega 规格： 20μg 二周到货 生化试验

说明  
 核受体检测可以通过传统的方法即利用上游带有核受体反应元件的最小启动子载体来完成。您也可以选择病毒元件像小鼠乳腺肿瘤病毒长末段重复启动子来检测雌激素或糖皮质激素的响应( 如pGL4.36)。很多情况下，利用这些方法进行的研究需要选择含有合适的内源核受体的细胞系，就是说每一种核受体的研究可能需要选择不同的细胞系。利用酵母双杂交原理的方法也适用于核受体的研究。核受体配基结合域与GAL4 DNA 结合域相融合，通过上游含有GAL4 上游激活序列的重复片段的最小启动子的萤火虫萤光素酶载体转染至细胞中。配基结合区域会与配基结合形成同源或异源的二聚体，以及与激活因子或抑制因子相互作用。这种单杂交系统可帮助您在很多细胞系中研究您所感兴趣的核受体。
特点    • 强劲：基于GAL4 的系统从内源性受体中去除了背景信号。
• 更灵敏：9X Gal4 经过优化，反应性增强，信噪比更高。
• 适应性强：海肾/ 新霉素的组合标记的引入，使得只需一个载体，既可用双萤光素酶检测试剂盒进行归一化，又可用选择性标记筛选生成的稳定细胞系。
• 稳定如一：仅需使用一个实验体系，就可以将所有的核受体进行比较或者研究其特征。
• 更快得到结果：去稳定化的luc2P 的萤光素酶报告基因经过优化，具有更高的灵敏度和更短的诱导时间。

 

E1370   pGL4.35[luc2P/9XGAL4UAS/Hygro]Vector   品牌 Promega

发表于由

pGL4.35[luc2P/9XGAL4UAS/Hygro]Vector

货号： E1370      产品名称： pGL4.35[luc2P/9XGAL4UAS/Hygro]Vector   品牌： Promega 规格： 20μg 二周到货 生化试验

说明  
 核受体检测可以通过传统的方法即利用上游带有核受体反应元件的最小启动子载体来完成。您也可以选择病毒元件像小鼠乳腺肿瘤病毒长末段重复启动子来检测雌激素或糖皮质激素的响应( 如pGL4.36)。很多情况下，利用这些方法进行的研究需要选择含有合适的内源核受体的细胞系，就是说每一种核受体的研究可能需要选择不同的细胞系。利用酵母双杂交原理的方法也适用于核受体的研究。核受体配基结合域与GAL4 DNA 结合域相融合，通过上游含有GAL4 上游激活序列的重复片段的最小启动子的萤火虫萤光素酶载体转染至细胞中。配基结合区域会与配基结合形成同源或异源的二聚体，以及与激活因子或抑制因子相互作用。这种单杂交系统可帮助您在很多细胞系中研究您所感兴趣的核受体。
特点    • 强劲：基于GAL4 的系统从内源性受体中去除了背景信号。
• 更灵敏：9X Gal4 经过优化，反应性增强，信噪比更高。
• 适应性强：海肾/ 新霉素的组合标记的引入，使得只需一个载体，既可用双萤光素酶检测试剂盒进行归一化，又可用选择性标记筛选生成的稳定细胞系。
• 稳定如一：仅需使用一个实验体系，就可以将所有的核受体进行比较或者研究其特征。
• 更快得到结果：去稳定化的luc2P 的萤光素酶报告基因经过优化，具有更高的灵敏度和更短的诱导时间。

 
 

 

pseth-lacz   pSELECT-hygro-LacZ   品牌 Invivogen

发表于由

pSELECT-hygro-LacZ

货号： pseth-lacz      产品名称： pSELECT-hygro-LacZ   品牌： Invivogen 规格： 20 μg 三周到货 生化试验

pSELECT-hygro
pSELECT-hygro plasmids offer all the features necessary to express a gene of interest at high levels in a large number of cell types. pSELECT-hygro plasmids contain two transcription units, the first drives the expression of the gene of interest and the second drives the expression of a hygromycin selectable marker. pSELECT-hygro contains a multiple cloning site (MCS) downstream of the composite promoter for convenient cloning of a gene of interest. pSELECT-hygro is also available with the LacZ reporter gene and can be used as control vector.
– No transcriptional interference: the two expression cassettes are oriented in convergent directions
– Strong and ubiquitous expression of the transgene: EF1α/HTLV composite promoter
– Convenient cloning of a gene of interest with a Multiple Cloning Site
– Selectable with hygromycin in mammalian cells and E.coli

pseth-mcs   pSELECT-hygro-mcs   品牌 Invivogen

发表于由

pSELECT-hygro-mcs

货号： pseth-mcs      产品名称： pSELECT-hygro-mcs   品牌： Invivogen 规格： 20 μg 三周到货 生化试验

pSELECT-hygro
pSELECT-hygro plasmids offer all the features necessary to express a gene of interest at high levels in a large number of cell types. pSELECT-hygro plasmids contain two transcription units, the first drives the expression of the gene of interest and the second drives the expression of a hygromycin selectable marker. pSELECT-hygro contains a multiple cloning site (MCS) downstream of the composite promoter for convenient cloning of a gene of interest. pSELECT-hygro is also available with the LacZ reporter gene and can be used as control vector.
– No transcriptional interference: the two expression cassettes are oriented in convergent directions
– Strong and ubiquitous expression of the transgene: EF1α/HTLV composite promoter
– Convenient cloning of a gene of interest with a Multiple Cloning Site
– Selectable with hygromycin in mammalian cells and E.coli

pvitro1-gfplacz   pVITRO1-hygro-GFP/LacZ   品牌 Invivogen

发表于由

pVITRO1-hygro-GFP/LacZ

货号： pvitro1-gfplacz      产品名称： pVITRO1-hygro-GFP/LacZ   品牌： Invivogen 规格： 20 μg 三周到货 生化试验

pVITRO1-GFP/LacZ
Expression plasmids pVITRO1-GFP/LacZ are developed mainly for in vitro studies. pVITRO1-GFP/LacZ allow the ubiquitous and constitutive co-expression of two reporter genes: GFP (green fluorescent protein) and LacZ (β-galactosidase).
pVITRO1-GFP/LacZ plasmids can be stably transfected in mammalian cells and the reporter genes are expressed at high levels.
pVITRO1-GFP/LacZ plasmids carry two elongation factor 1 alpha (EF-1α) promoters, from rat and mouse origins combined to the CMV and SV40 enhancers respectively. pVITRO1-GFP/LacZ plasmids are available with different selectable markers that are active both in E. coli and mammalian cells.
Promoters: CMV enhancer / rat EF-1α & SV40 enhancer / mouse EF-1α
Selection markers active both in E. coli and mammalian cells:
– bsr (blasticidin resistance)
– hph (hygromycin resistance)
– neo (kanamycin/G418 resistance).
Reporter Genes:
– GFP (green fluorescent protein)
– LacZ (β-galactosidase)

pvitro1-mcs   pVITRO1-hygro-mcs   品牌 Invivogen

发表于由

pVITRO1-hygro-mcs

货号： pvitro1-mcs      产品名称： pVITRO1-hygro-mcs   品牌： Invivogen 规格： 20 μg 三周到货 生化试验

pVITRO1-MCS
Expression plasmids pVITRO1-MCS are developed mainly for in vitro studies. pVITRO1-MCS allow the ubiquitous and constitutive co-expression of two genes of interest.
pVITRO1-MCS plasmids can be stably transfected in mammalian cells and the genes of interest are expressed at high levels.
pVITRO1-MCS plasmids carry two elongation factor 1 alpha (EF-1α) promoters, from rat and mouse origins combined to the CMV and SV40 enhancers respectively. pVITRO1-MCS plasmids are available with different selectable markers that are active both in E. coli and mammalian cells.
pVITRO1-mcs plasmids contain two multiple cloning sites (MCS) for the convenient cloning of your cDNAs of interest.
Promoters: CMV enhancer / rat EF-1α & SV40 enhancer / mouse EF-1α
Selection markers active both in E. coli and mammalian cells:
– bsr (blasticidin resistance)
– hph (hygromycin resistance)
– neo (kanamycin/G418 resistance).
Cloning sites:
– MCS1: 5’- Bsp EI, Bst 1107I, Bam HI, Bsi WI, Avr II -3’
– MCS2: 5’- Age I, Eco RV, Bgl II, Bsr GI, Nhe I -3’

pvitro2-gfplacz   pVITRO2-hygro-GFP/LacZ   品牌 Invivogen

发表于由

pVITRO2-hygro-GFP/LacZ

货号： pvitro2-gfplacz      产品名称： pVITRO2-hygro-GFP/LacZ   品牌： Invivogen 规格： 20 μg 三周到货 生化试验

pVITRO2-GFP/Lacz
Expression plasmids pVITRO2-GFP/Lacz are developed mainly for in vitro studies.
pVITRO2-GFP/Lacz allows the ubiquitous and constitutive co-expression of two reporter genes: GFP (green fluorescent protein) and LacZ (β-galactosidase). pVITRO2-GFP/Lacz plasmids can be stably transfected in mammalian cells and the reporter genes are expressed at high levels.
pVITRO2-GFP/Lacz plasmids carry two human ferritin composite promoters, FerH (heavy chain) and FerL (light chain). To eliminate the iron regulation, their 5’UTRs have been replaced by the 5’UTR of the mouse and chimpanzee EF-1α genes. pVITRO2-GFP/Lacz plasmids are available with different selectable markers that are active both in E. coli and mammalian cells.
Promoters: SV40/hFerH/mEF1α & CMV/hFerL/chEF1α
Selection markers active both in E. coli and mammalian cells:
– bsr (blasticidin resistance)
– hph (hygromycin resistance)
– neo (kanamycin/G418 resistance).
Reporter Genes:
– GFP (green fluorescent protein)
– LacZ (β-galactosidase)

 

 

pvitro2-mcs   pVITRO2-hygro-mcs   品牌 Invivogen

发表于由

pVITRO2-hygro-mcs

货号： pvitro2-mcs      产品名称： pVITRO2-hygro-mcs   品牌： Invivogen 规格： 20 μg 三周到货 生化试验

pVITRO2-MCS
Expression plasmids pVITRO2-MCS are developed mainly for in vitro studies.
pVITRO2-MCS allows the ubiquitous and constitutive co-expression of two genes of interest.
pVITRO2-MCS plasmids carry two human ferritin composite promoters, FerH (heavy chain) and FerL (light chain). To eliminate the iron regulation, their 5’UTRs have been replaced by the 5’UTR of the mouse and chimpanzee EF-1α genes. pVITRO2-MCS plasmids are available with different selectable markers that are active both in E. coli and mammalian cells.
pVITRO2-MCS plasmids contain two multiple cloning sites (MCS) for the convenient cloning of your cDNAs of interest.
Promoters: SV40/hFerH/mEF1α & CMV/hFerL/chEF1α
Selection markers active both in E. coli and mammalian cells:
– bsr (blasticidin resistance)
– hph (hygromycin resistance)
– neo (kanamycin/G418 resistance).
Cloning sites:
– MCS1: 5’- Age I, Eco RV, Bam HI, Mlu I, Cla I, Sal I, Avr II -3’
– MCS2: 5’- Sgr AI, Fsp I, Bgl II, Eco RI, Bst BI, Xho I, Nhe I -3’

pvitro1-gfpsp   pVITRO1-hygro-GFP/SEAP   品牌 Invivogen

发表于由

pVITRO1-hygro-GFP/SEAP

货号： pvitro1-gfpsp      产品名称： pVITRO1-hygro-GFP/SEAP   品牌： Invivogen 规格： 20 μg 三周到货 生化试验

pVITRO1-GFP/SEAP
Expression plasmids pVITRO1-GFP/SEAP are developed mainly for in vitro studies.
pVITRO1-GFP/SEAP allow the ubiquitous and constitutive co-expression of two reporter genes: GFP (green fluorescent protein) and SEAP (secreted embryonic alkaline phosphatase).
pVITRO1-GFP/SEAP plasmids can be stably transfected in mammalian cells and the reporter genes are expressed at high levels.
pVITRO1-GFP/SEAP plasmids carry two elongation factor 1 alpha (EF-1α) promoters, from rat and mouse origins combined to the CMV and SV40 enhancers respectively. pVITRO1-GFP/SEAP plasmids are available with different selectable markers that are active both in E. coli and mammalian cells.
Promoters: CMV enhancer / rat EF-1α & SV40 enhancer / mouse EF-1α
Selection markers active both in E. coli and mammalian cells:
– bsr (blasticidin resistance)
– hph (hygromycin resistance)
– neo (kanamycin/G418 resistance).
Reporter Genes:
– GFP (green fluorescent protein)
– SEAP (secreted embryonic alkaline phosphatase)

pvitro1-lucsp   pVITRO1-hygro-Lucia/SEAP   品牌 Invivogen

发表于由

pVITRO1-hygro-Lucia/SEAP

货号： pvitro1-lucsp      产品名称： pVITRO1-hygro-Lucia/SEAP   品牌： Invivogen 规格： 20 μg 三周到货 生化试验

pVITRO1-Lucia/SEAP
Expression plasmids pVITRO1-Lucia/SEAP are developed mainly for in vitro studies.
pVITRO1-Lucia/SEAP allow the ubiquitous and constitutive co-expression of two secreted reporter genes: a secreted luciferase (Lucia) and SEAP (secreted embryonic alkaline phosphatase).
pVITRO1-Lucia/SEAP plasmids can be stably transfected in mammalian cells and the reporter genes are expressed at high levels.
pVITRO1-Lucia/SEAP plasmids carry two elongation factor 1 alpha (EF-1α) promoters, from rat and mouse origins combined to the CMV and SV40 enhancers respectively.  pVITRO1-Lucia/SEAP plasmids are available with different selectable markers that are active both in E. coli and mammalian cells.
Promoters: CMV enhancer / rat EF-1α & SV40 enhancer / mouse EF-1α
Selection markers active both in E. coli and mammalian cells:
– bsr (blasticidin resistance)
– hph (hygromycin resistance)
– neo (kanamycin/G418 resistance).
Reporter Genes:
– Lucia secreted luciferase
– SEAP (secreted embryonic alkaline phosphatase)
These products are covered by a Limited Use License (See Terms and Conditions).

pvitro2-gfpsp   pVITRO2-hygro-GFP/SEAP   品牌 Invivogen

发表于由

pVITRO2-hygro-GFP/SEAP

货号： pvitro2-gfpsp      产品名称： pVITRO2-hygro-GFP/SEAP   品牌： Invivogen 规格： 20 μg 三周到货 生化试验

pVITRO2-GFP/SEAP
Expression plasmids pVITRO2-GFP/SEAP are developed mainly for in vitro studies.
pVITRO2-GFP/SEAP allows the ubiquitous and constitutive co-expression of two reporter genes: GFP (green fluorescent protein) and SEAP (secreted embryonic alkaline phosphatase). pVITRO2-GFP/SEAP plasmids can be stably transfected in mammalian cells and the reporter genes are expressed at high levels.
pVITRO2-GFP/SEAP plasmids carry two human ferritin composite promoters, FerH (heavy chain) and FerL (light chain). To eliminate the iron regulation, their 5’UTRs have been replaced by the 5’UTR of the mouse and chimpanzee EF-1α genes. pVITRO2-GFP/SEAP plasmids are available with different selectable markers that are active both in E. coli and mammalian cells.
Promoters: SV40/hFerH/mEF1α & CMV/hFerL/chEF1α
Selection markers active both in E. coli and mammalian cells:
– bsr (blasticidin resistance)
– hph (hygromycin resistance)
– neo (kanamycin/G418 resistance).
Reporter Genes:
– GFP (green fluorescent protein)
– SEAP (secreted embryonic alkaline phosphatase)

 

pvitro2-lucsp   pVITRO2-hygro-Lucia/SEAP   品牌 Invivogen

发表于由

pVITRO2-hygro-Lucia/SEAP

货号： pvitro2-lucsp      产品名称： pVITRO2-hygro-Lucia/SEAP   品牌： Invivogen 规格： 20 μg 三周到货 生化试验

pVITRO2-Lucia/SEAP
Expression plasmids pVITRO2-Lucia/SEAP are developed mainly for in vitro studies.
pVITRO2-Lucia/SEAP allows the ubiquitous and constitutive co-expression of two reporter genes: Lucia (secreted luciferase) and SEAP (secreted embryonic alkaline phosphatase). pVITRO2-Lucia/SEAP plasmids can be stably transfected in mammalian cells and the reporter genes are expressed at high levels.
pVITRO2-Lucia/SEAP plasmids carry two human ferritin composite promoters, FerH (heavy chain) and FerL (light chain). To eliminate the iron regulation, their 5’UTRs have been replaced by the 5’UTR of the mouse and chimpanzee EF-1α genes. pVITRO2-Lucia/SEAP plasmids are available with different selectable markers that are active both in E. coli and mammalian cells.
Promoters: SV40/hFerH/mEF1α & CMV/hFerL/chEF1α
Selection markers active both in E. coli and mammalian cells:
– bsr (blasticidin resistance)
– hph (hygromycin resistance)
– neo (kanamycin/G418 resistance).
Reporter Genes:
– Lucia luciferase (secreted luciferase)
– SEAP (secreted embryonic alkaline phosphatase)
These products are covered by a Limited Use License (See Terms and Conditions).