HOM DIP COMPLETE SET
货号: 95401.H1R3 产品名称: HOM DIP COMPLETE SET 品牌: Invitrogen 规格: ***
![](http://thermofisher.jinpanbio.com/wp-content/uploads/2024/04/BZWZ.jpg)
V1741 PI3K (p110α [H1047R]/p85α), 20μg 品牌 Promega
PI3K (p110α [H1047R]/p85α), 20μg
货号: V1741 产品名称: PI3K (p110α [H1047R]/p85α), 20μg 品牌: Promega 规格: 200μl 二周到货 生化试验
说明
磷脂酰肌醇( PI ) 及 其磷酸化衍生物,统称为 磷酸肌醇( Phosphoinositides ) ,是重要的第二信使,而第二信使作为信号分子在细胞膜重构中起关键作用。它们由一类被称为磷酸肌醇脂质激酶( Phosphoinositide Lipid Kinases , PIKs )的激酶家族催化产生。在哺乳动物中,已确定 19 个 PIK 亚型。根据其对磷酸化肌醇环第 3 、 4 、 5 位羟基基团磷酸化偏好, 大致分为三个主要的家族:磷酸肌醇 3 – 激酶( PI3Ks ),磷脂酰肌醇 4- 激酶( PI4Ks )和磷酸肌醇磷酸 – 激酶( PIP5Ks 和 PIP4Ks )。
Promega 脂质激酶活性检测系统,利用 ADP-Glo Kinase Assay 激酶活性发光检测平台,提供脂质激酶、底物和 ADP-Glo 检测试剂,为脂质激酶反应提供了整套完备试剂。这些试剂包括纯化的人类 I 类 PI3Ks 重组蛋白,优化的反应缓冲液和即用型脂质激酶底物。
可单独购买酶,也可购买整套完备试剂盒 PI3K-Glo™ Class I Profiling Kit ,组分包括 PI3Ks (α、β、γ、δ,各 5 μ g ), PIP2 : 3PS 脂质激酶底物( 0.25mg )和 ADP-Glo ™激酶检测试剂盒 (1,000 次 ) 。底物以冷冻小 单层 脂质 体形式提供,为磷脂酰肌醇( PI )或磷酸肌醇 -4,5- 二磷酸( PIP2 )与载脂磷脂酰丝氨酸( PS )的混合物,两者混合比例为 1:3 。 PI 与 PS 1:3 混合优化后的底物可用于 I 类 PI3Ks 。已证实, PI 与 PS 1:3 混合后的底物可被大多数 PI3K 家族成员识别,可做为通用型 PI 脂质激酶底物。
脂质激酶活性检测原理如图 1 所示。将脂质激酶底物( 1 PI : 3 PS 或 1 PIP2 : 3 PS )、重组脂质激酶和 ATP 共同孵育进行脂质激酶反应,之后用 ADP-Glo ™激酶检测试剂盒检测激酶活性。 ADP-Glo ™激酶检测试剂盒操作分两个步骤: 1 )激酶反应后,加入 ATP 耗竭试剂终止激酶反应,同时耗尽所有剩余 ATP ,只留下 ADP ; 2 )加入激酶检测试剂,将 ADP 转变为 ATP ,同时在偶联的萤光素酶 / 萤光素反应中将 ATP 转变为光信号。
特点 • 提供 I 类 PI3Ks 活性检测完整解决方案:
• 纯化的人源重组酶,高度特异活性
• 即用型脂质底物( PI 或 PIP2 )
• 通用反应缓冲液
• 高灵敏度检测
• 选择性卓越: 即使 % 底物转化率很低,仍可获得高信噪比
• 结果可靠: 动态范围宽、背景低、灵敏度高,减少了数据的不可靠性
• 节约时间: 均质检测,简便的 " 加样 – 读数 " 模式
• 避免假阳性结果: 特殊配方和发光检测降低了假阳性率
• 节约经费: 可轻松升级至 1,536 孔板检测模式,降低每孔检测成本
![](http://thermofisher.jinpanbio.com/wp-content/uploads/2024/04/BZWZ.jpg)
BML-HR005-0050 (±)5-Hydroxyeicosa-6E,8Z,11Z,14Z-tetraenoic acid 品牌 Biomol
(±)5-Hydroxyeicosa-6E,8Z,11Z,14Z-tetraenoic acid
货号: BML-HR005-0050 产品名称: (±)5-Hydroxyeicosa-6E,8Z,11Z,14Z-tetraenoic acid 品牌: Biomol 规格: 50 ug 3周到货 生化实验
Biosynthesis via rat hepatic microsomal cytochrome P-450. Racemic chromatographic reference standard.
Product Specification
CAS:73307-52-5
Formulation:Supplied at 50 µg/ml in ethanol.
Purity:98%
Long Term Storage:-80°C
-80°C
![](http://thermofisher.jinpanbio.com/wp-content/uploads/2024/04/BZWZ.jpg)
BML-HR005-1000 (±)5-Hydroxyeicosa-6E,8Z,11Z,14Z-tetraenoic acid 品牌 Biomol
(±)5-Hydroxyeicosa-6E,8Z,11Z,14Z-tetraenoic acid
货号: BML-HR005-1000 产品名称: (±)5-Hydroxyeicosa-6E,8Z,11Z,14Z-tetraenoic acid 品牌: Biomol 规格: 1 mg 3周到货 生化实验
Biosynthesis via rat hepatic microsomal cytochrome P-450. Racemic chromatographic reference standard.
Product Specification
CAS:73307-52-5
Formulation:Supplied at 50 µg/ml in ethanol.
Purity:98%
Long Term Storage:-80°C
-80°C
![](http://thermofisher.jinpanbio.com/wp-content/uploads/2024/04/BZWZ.jpg)
BML-HR012-1000 (±)12-Hydroxyeicosa-5Z,8Z,10E,14Z-tetraenoic acid (HETE) 品牌 Biomol
(±)12-Hydroxyeicosa-5Z,8Z,10E,14Z-tetraenoic acid (HETE)
货号: BML-HR012-1000 产品名称: (±)12-Hydroxyeicosa-5Z,8Z,10E,14Z-tetraenoic acid (HETE) 品牌: Biomol 规格: 1 mg
![](http://thermofisher.jinpanbio.com/wp-content/uploads/2024/04/BZWZ.jpg)
BML-HR013-0050 (±)13-Hydroxyoctadeca-9Z,11E-dienoic acid 品牌 Biomol
(±)13-Hydroxyoctadeca-9Z,11E-dienoic acid
货号: BML-HR013-0050 产品名称: (±)13-Hydroxyoctadeca-9Z,11E-dienoic acid 品牌: Biomol 规格: 50 ug 3周到货 生化实验
Product Specification
MW:296.5
CAS:73804-64-5
Formulation:Liquid. 50µg/ml in ethanol.
Purity:99%
Long Term Storage:-20°C
-20°C
![](http://thermofisher.jinpanbio.com/wp-content/uploads/2024/04/BZWZ.jpg)
BML-HR013-1000 (±)13-Hydroxyoctadeca-9Z,11E-dienoic acid 品牌 Biomol
(±)13-Hydroxyoctadeca-9Z,11E-dienoic acid
货号: BML-HR013-1000 产品名称: (±)13-Hydroxyoctadeca-9Z,11E-dienoic acid 品牌: Biomol 规格: 1 mg 3周到货 生化实验
Product Specification
MW:296.5
CAS:73804-64-5
Formulation:Liquid. 50µg/ml in ethanol.
Purity:99%
Long Term Storage:-20°C
-20°C
![](http://thermofisher.jinpanbio.com/wp-content/uploads/2024/04/BZWZ.jpg)
BML-HR505-0050 (±)5-Hydroxyeicosa-6E,8Z,11Z,14Z,17Z-pentaenoic acid 品牌 Biomol
(±)5-Hydroxyeicosa-6E,8Z,11Z,14Z,17Z-pentaenoic acid
货号: BML-HR505-0050 产品名称: (±)5-Hydroxyeicosa-6E,8Z,11Z,14Z,17Z-pentaenoic acid 品牌: Biomol 规格: 50 ug 3周到货 生化实验
Product Specification
CAS:83952-40-3
Formulation:Liquid. 50 µg/ml in ethanol.
Purity:98%
Long Term Storage:-80°C
-80°C
![](http://thermofisher.jinpanbio.com/wp-content/uploads/2024/04/BZWZ.jpg)
ASB-ARP32907-P050 PAb to HR 品牌 AXXORA
PAb to HR
货号: ASB-ARP32907-P050 产品名称: PAb to HR 品牌: AXXORA 规格: 50 ug
![](http://thermofisher.jinpanbio.com/wp-content/uploads/2024/04/BZWZ.jpg)
ASB-ARP32908-P050 PAb to HR 品牌 AXXORA
PAb to HR
货号: ASB-ARP32908-P050 产品名称: PAb to HR 品牌: AXXORA 规格: 50 ug
![](http://thermofisher.jinpanbio.com/wp-content/uploads/2024/04/BZWZ.jpg)
9236440 0.5 hr 品牌 Qiagen
0.5 hr
货号: 9236440 产品名称: 0.5 hr 品牌: Qiagen 规格: ***
CHI-HR-200CCL5-C010 CCL5 (human) (rec.) (His) 品牌 Chimerigen
CCL5 (human) (rec.) (His)
货号: CHI-HR-200CCL5-C010 产品名称: CCL5 (human) (rec.) (His) 品牌: Chimerigen 规格: 10 μg
![](http://thermofisher.jinpanbio.com/wp-content/uploads/2024/04/BZWZ.jpg)
CHI-HR-200CCL5-C050 CCL5 (human) (rec.) (His) 品牌 Chimerigen
CCL5 (human) (rec.) (His)
货号: CHI-HR-200CCL5-C050 产品名称: CCL5 (human) (rec.) (His) 品牌: Chimerigen 规格: 50 μg
![](http://thermofisher.jinpanbio.com/wp-content/uploads/2024/04/BZWZ.jpg)
CHI-HR-20016-C010 IL-16 (human) (rec.) (His) 品牌 Chimerigen
IL-16 (human) (rec.) (His)
货号: CHI-HR-20016-C010 产品名称: IL-16 (human) (rec.) (His) 品牌: Chimerigen 规格: 10 μg
![](http://thermofisher.jinpanbio.com/wp-content/uploads/2024/04/BZWZ.jpg)
CHI-HR-20016-C050 IL-16 (human) (rec.) (His) 品牌 Chimerigen
IL-16 (human) (rec.) (His)
货号: CHI-HR-20016-C050 产品名称: IL-16 (human) (rec.) (His) 品牌: Chimerigen 规格: 50 μg
![](http://thermofisher.jinpanbio.com/wp-content/uploads/2024/04/BZWZ.jpg)
CHI-HR-20017F-C010 IL-17F (human) (rec.) (His) 品牌 Chimerigen
IL-17F (human) (rec.) (His)
货号: CHI-HR-20017F-C010 产品名称: IL-17F (human) (rec.) (His) 品牌: Chimerigen 规格: 10 μg
![](http://thermofisher.jinpanbio.com/wp-content/uploads/2024/04/BZWZ.jpg)
CHI-HR-20017F-C050 IL-17F (human) (rec.) (His) 品牌 Chimerigen
IL-17F (human) (rec.) (His)
货号: CHI-HR-20017F-C050 产品名称: IL-17F (human) (rec.) (His) 品牌: Chimerigen 规格: 50 μg
![](http://thermofisher.jinpanbio.com/wp-content/uploads/2024/04/BZWZ.jpg)
CHI-HR-200BMP2-C010 BMP-2 (human) (rec.) (His) 品牌 Adipogen
BMP-2 (human) (rec.) (His)
货号: CHI-HR-200BMP2-C010 产品名称: BMP-2 (human) (rec.) (His) 品牌: Adipogen 规格: 10 ug
![](http://thermofisher.jinpanbio.com/wp-content/uploads/2024/04/BZWZ.jpg)
CHI-HR-200BMP2-C050 BMP-2 (human) (rec.) (His) 品牌 Adipogen
BMP-2 (human) (rec.) (His)
货号: CHI-HR-200BMP2-C050 产品名称: BMP-2 (human) (rec.) (His) 品牌: Adipogen 规格: 50 ug
![](http://thermofisher.jinpanbio.com/wp-content/uploads/2024/04/BZWZ.jpg)
CHI-HR-200CCL5-C010 CCL5 (human) (rec.) (His) 品牌 Adipogen
CCL5 (human) (rec.) (His)
货号: CHI-HR-200CCL5-C010 产品名称: CCL5 (human) (rec.) (His) 品牌: Adipogen 规格: 10 ug
![](http://thermofisher.jinpanbio.com/wp-content/uploads/2024/04/BZWZ.jpg)