标签： evi

Anti-EVI-5

货号： ABN194      产品名称： Anti-EVI-5   品牌： Millipore 规格： EA 三周到货 生化实验

Anti-EVI-5
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Species Reactivity Key Applications Host Format Antibody Type
M, R, H  IHC, WB Rabbit Affinity Purified Polyclonal Antibody
Description:
Anti-EVI-5 Antibody
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Molecular Weight:
~95 kDa observed. Uncharacterized bands may be observed at ~31 kDa, ~17 kDa, and ~15 kDa in some cell lysates. This protein is known to have three potential phosphorylation sites.
Immunogen:
KLH-conjugated linear peptide corresponding to the human EVI-5.
Background Information:
Also known as Ecotropic viral integration site 5 protein homolog, EVI-5 serves as a key regulator of the cell cycle through time-dependent stabilization of Emi1, FBX05 protein, and APC/C ubiquitination substrates, such as cyclin A. EVI-5 accumulates in early G1 following Plk1 destruction, but is degraded in a Plk1- and ubiquitin-dependent manner in early mitosis. EVI-5 ablation causes premature APC/C activation, SCF(betaTrCP)-dependent Emi1 degradation, and cyclin destruction, all of which leads to cell cycle arrest and cytokinesis failure. It exists as a monomer and a dimer, and it may form complexes with alpha- and gamma-tubulin or interact with the INCENP-aurora B kinase-survivin chromosomal passenger complex. EVI-5 is implicated in abnormal states, such as multiple sclerosis and neuroblastoma.
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Species Reactivity:
Mouse
Rat
Human
Species Reactivity Note:
Demonstrated to react with Mouse and Rat. Predicted to react with Human based upon immunogen design.
Application Notes:
Western Blot Analysis: 2 µg/mL from a representative lot detected EVI-5 in NIH/3T3 nuclear extract.
Control:
Mouse and rat brain tissues
Quality Assurance:
Evaluated by Immunohistochemistry in mouse and rat brain tissues.
Immunohistochemistry Analysis: A 1:500 dilution of this antibody detected EVI-5 in mouse and rat brain tissues.
Purification Method:
Affinity purified
Presentation:
Purified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Storage Conditions:
Stable for 1 year at 2-8°C from date of receipt.
UniProt Number:
O60447
Entrez Gene Number:
NP_005656
Gene Symbol:
EVI5
NB4S
Alternate Names:
Ecotropic viral integration site 5 protein homolog
EVI-5
Usage Statement:
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Key Applications:
Immunohistochemistry
Western Blotting
Product Name:
Anti-EVI-5
Concentration:
Please refer to the Certificate of Analysis for the lot-specific concentration.
Antibody Type:
Polyclonal Antibody
Qty/Pk:
100 μg
Format:
Affinity Purified
Host:
Rabbit
Applications:
This EVI-5 antibody is validated for use in IHC & WB for the detection of the EVI-5 protein.

2-8℃