标签： cul

BML-PW0190-0025   CUL1 (Arabidopsis thaliana), pAb   品牌 Biomol

CUL1 (Arabidopsis thaliana), pAb

货号： BML-PW0190-0025      产品名称： CUL1 (Arabidopsis thaliana), pAb   品牌： Biomol 规格： 25 ul 3周到货 生化实验

Product Specification
Alternative Name:AtCUL1
Host:Rabbit
Immunogen:Full length recombinant AtCUL1.
UniProt ID:Q94AH6
Application:Western Blot (1:1000)
Optimal conditions must be determined individually for each application.
Specificity:Recognizes Arabidopsis CUL1.
Formulation:Liquid. Neat serum containing 0.1% sodium azide.
Use/Stability:Store unopened vial at -20°C until required for use. Store diluted antibody at 4°C (do not freeze) and use within 1 month.
Handling:Avoid freeze/thaw cycles.
Short Term Storage:-20°C
Long Term Storage:-20°C
Miscellaneous/General:A series of studies in Caenorhabditis elegans, Schizosaccharomyces pombe and mammals has shed light on the important roles that cullin-based ubiquitin ligases play in the regulation of diverse developmental processes and environmental responses in eukaryotic organisms.The ubiquitin-like protein RUB1 (NEDD8 in mammals) is conjugated to target proteins by a mechanism similar to that of ubiquitin conjugation in Arabidopsis. It has been shown that the Skp1-Cul1/Cdc53-F-box (SCF) subunit CUL1 is modified by RUB1 in vivo. The formation of RUB-AtCUL1 is dependent on AXR1 and ECR1 and expression of these proteins is restricted to zones of active cell division and cell elongation, consistent with their role in growth regulation. These results provide strong support for a model in which RUB conjugation of AtCUL1 affects the function of SCF E3s.
 

-20°C

BML-PW0190-0100   CUL1 (Arabidopsis thaliana), pAb   品牌 Biomol

CUL1 (Arabidopsis thaliana), pAb

货号： BML-PW0190-0100      产品名称： CUL1 (Arabidopsis thaliana), pAb   品牌： Biomol 规格： 100 ul 3周到货 生化实验

Product Specification
Alternative Name:AtCUL1
Host:Rabbit
Immunogen:Full length recombinant AtCUL1.
UniProt ID:Q94AH6
Application:Western Blot (1:1000)
Optimal conditions must be determined individually for each application.
Specificity:Recognizes Arabidopsis CUL1.
Formulation:Liquid. Neat serum containing 0.1% sodium azide.
Use/Stability:Store unopened vial at -20°C until required for use. Store diluted antibody at 4°C (do not freeze) and use within 1 month.
Handling:Avoid freeze/thaw cycles.
Short Term Storage:-20°C
Long Term Storage:-20°C
Miscellaneous/General:A series of studies in Caenorhabditis elegans, Schizosaccharomyces pombe and mammals has shed light on the important roles that cullin-based ubiquitin ligases play in the regulation of diverse developmental processes and environmental responses in eukaryotic organisms.The ubiquitin-like protein RUB1 (NEDD8 in mammals) is conjugated to target proteins by a mechanism similar to that of ubiquitin conjugation in Arabidopsis. It has been shown that the Skp1-Cul1/Cdc53-F-box (SCF) subunit CUL1 is modified by RUB1 in vivo. The formation of RUB-AtCUL1 is dependent on AXR1 and ECR1 and expression of these proteins is restricted to zones of active cell division and cell elongation, consistent with their role in growth regulation. These results provide strong support for a model in which RUB conjugation of AtCUL1 affects the function of SCF E3s.
 

-20°C

ABN289   Anti-Cullin-5 (CUL5)   品牌 Millipore

Anti-Cullin-5 (CUL5)

货号： ABN289      产品名称： Anti-Cullin-5 (CUL5)   品牌： Millipore 规格： EA 三周到货 生化实验

Anti-Cullin-5 (CUL5)
Recommended Replacement for: AB10622
Species Reactivity Key Applications Host Format Antibody Type
H  WB, IHC Rabbit Affinity Purified Polyclonal Antibody
Description:
Anti-Cullin-5 (CUL5) Antibody
Replaces:
AB10622
Specificity:
This antibody recognizes Cullin-5 near the N-Terminus.
Molecular Weight:
~90 kDa observed. Neddylation of Cullin-5 at a.a. 724 with NEDD8 results in a 10 kDa increase in molecular weight (Hori. T., et al. (1999). Oncogene. 18(48):6829-6834).
Epitope:
Near N-Terminus
Immunogen:
KLH-conjugated linear peptide corresponding to human Cullin-5 near the Near N-Terminus.
Background Information:
CUL-5/VACM-1 (cullin-5, vasopressin-activated calcium mobilizing receptor 1) is one component of the ECS (elongin B/C-cul2/cul5-SOCS box protein) complex that forms an E3 ubiquitin ligase that covalently binds ubiquitin to proteins targeted for degradation via the proteasome. CUL-5 is ubiquitously expressed in the central nervous system and in many peripheral organs. Human immunodeficiency virus-1 (HIV-1) protein Vif forms a Skp1-cullin-F-box (SCF)-like complex with CUL-5/VACM-1 and Elongins B and C. The ability of Vif to suppress the antiviral activity of APOBEC3G, which induces hypermutations in newly synthesized viral DNA, is specifically dependent on CUL-5-SCF function, as it allows Vif to interact with APOBEC3G and induce its ubiquitination and degradation. The CUL-5-SCF pathway used by Vif is thus a potential target for antiviral development. CUL-5/VACM-1 inhibits cellular growth by a mechanism that involves MAPK and p53 signaling pathways. In human tissues expression in kidney, brain, heart, placenta, and skeletal muscle is observed. CUL-5/VACM-1 is also expressed in both breast epithelial cells and breast cancer cells. Decreased expression of CUL-5/VACM-1 may play a role in breast tumorigenesis. Further evidence for this finding is observed in T47D breast cancer cells in which overexpression of CUL-5/VACM-1 significantly attenuates cellular proliferation and MAPK phosphorylation when compared to control cells.
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Species Reactivity:
Human
Species Reactivity Note:
Demonstrated to react with Human. Predicted to react with Rabbit based on 100% sequence homology. Other homologies: Feline (93% sequence homology) and Mouse and Rat (87% sequence homology).
Application Notes:
Immunohistochemistry Analysis: A 1:2,000 dilution from a representative lot detected Cullin-5 in human placenta and human testis tissue.
Control:
Human heart tissue lysate
Quality Assurance:
Evaluated by Western Blotting in human heart tissue lysate.
Western Blotting Analysis: 1.0 µg/mL of this antibody detected Cullin-5 in 10 µg of human heart tissue lysate.
Purification Method:
Antigen Affinity purified
Presentation:
Purified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Storage Conditions:
Stable for 1 year at 2-8°C from date of receipt.
UniProt Number:
Q93034
Entrez Gene Number:
NP_003469
Gene Symbol:
CUL5
VACM1
Alternate Names:
Cullin-5
CUL-5
Vasopressin-activated calcium-mobilizing receptor 1
VACM-1
Usage Statement:
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Key Applications:
Weste 2-8℃