标签归档：colorimetric

C0375-1 mg   Caspase 3, Substrate, Colorimetric   品牌 LKT

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Caspase 3, Substrate, Colorimetric

货号： C0375-1 mg      产品名称： Caspase 3, Substrate, Colorimetric   品牌： LKT 规格： 1 mg 三周到货 生化试验

Caspase 3, Substrate, Colorimetric     
Specifications 
Product ID  C0375
Product Name  Caspase 3, Substrate, Colorimetric
Formula Wt. 638.6
Store Temp +4oC
 

+4oC

C0375-2 mg   Caspase 3, Substrate, Colorimetric   品牌 LKT

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Caspase 3, Substrate, Colorimetric

货号： C0375-2 mg      产品名称： Caspase 3, Substrate, Colorimetric   品牌： LKT 规格： 2 mg 三周到货 生化试验

Caspase 3, Substrate, Colorimetric     
Specifications 
Product ID  C0375
Product Name  Caspase 3, Substrate, Colorimetric
Formula Wt. 638.6
Store Temp +4oC
 

+4oC

C0375-5 mg   Caspase 3, Substrate, Colorimetric   品牌 LKT

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Caspase 3, Substrate, Colorimetric

货号： C0375-5 mg      产品名称： Caspase 3, Substrate, Colorimetric   品牌： LKT 规格： 5 mg 三周到货 生化试验

Caspase 3, Substrate, Colorimetric    
Specifications 
Product ID  C0375
Product Name  Caspase 3, Substrate, Colorimetric
Formula Wt. 638.6
Store Temp +4oC
 

+4oC

K648-100   Glycogen Colorimetric Assay Kit II   品牌 Biovision

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Glycogen Colorimetric Assay Kit II

货号： K648-100      产品名称： Glycogen Colorimetric Assay Kit II   品牌： Biovision 规格： 100 assays 3周到货 生化实验

Glycogen Colorimetric Assay Kit II
Product Overview
Assay Kit Name:
Glycogen Colorimetric Assay Kit II
Kit Summary:
• Detection method- Absorbance (450 nm)
• Applications- The assay can detect less than 4 µg/ml of Glycogen in various tissues. Analysis of metabolism and cell signaling.
Sample Type:
• Animal tissues: Liver, muscle, etc.
• Cell culture: Adherent or suspension cells.
Features & Benefits:
• Simple, rapid, and high-throughput adaptable.
• Kit contains all necessary reagents for accurate measurement of Glycogen levels.
Kit Components:
• Glycogen Hydrolysis Buffer
• Gycogen Development Buffer
• Hydrolysis Enzyme Mix (lyophilized)
• Development Enzyme Mix (lyophilized)
• Probe (lyophilized)
• Glycogen Standard (2 mg/ml)
Description:
Glycogen serves as the main carbohydrate storage in animals and can be converted to glucose readily. It is primarily found in the liver and muscle tissues. Glycogen is a branched biopolymer comprising of α-1,4 linkage with α-1,6 linkages occurring every 8-10 glucose units along the backbone. Abnormal ability to utilize glycogen is found in diabetes and in several genetic glycogen storage diseases. Biovision’s Glycogen Assay kit II provides a simple, fast and robust way to measure Glycogen levels in various biological samples. This assay is suitable for measuring Glycogen levels in samples that contain reducing substances, which may interfere with the oxidase-based assays. In this assay, Glycogen is hydrolyzed into glucose, which is oxidized to form an intermediate that reduces a colorless Probe to a colored product with strong absorbance at 450 nm. This high-throughput suitable assay kit can detect less than 4 µg/ml of Glycogen in samples.
Storage Conditions:
-20°C
Shipping Conditions:
gel pack
USAGE: For Research Use Only! Not For Use in Humans.

-20°C

17-374   HDAC Assay,Colorimetric Det 96 Asy   品牌 Millipore

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HDAC Assay,Colorimetric Det 96 Asy

货号： 17-374      产品名称： HDAC Assay,Colorimetric Det 96 Asy   品牌： Millipore 规格： EA 三周到货 生化实验

HDAC Assay Kit, colorimetric detection
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PRODUCT FAMILY INFORMATION
HDAC Antibodies
Millipore’s HDAC Antibodies demonstrate specificity against Histone deacetylase. See below for antibodies, proteins and kits related to HDAC, based on the expertise of Upstate & Chemicon.
Millipore’s Anti-HDAC Antibodies demonstrates specificity against HDAC. See below for data, references and related products for HDAC. All Millipore antibodies are based on the expertise of Upstate & Chemicon.
» More…
Description:
HDAC Assay Kit, colorimetric detection
Trade Name:
Upstate (Millipore)
Qty/Pk:
96 assays
Product Overview:
This assay is a simple two-step procedure performed in a microtiter plate. In the first step, samples are incubated with the HDAC assay substrate, allowing deacetylation of the substrate. Next, the Activator Solution releases p-nitroanilide from the deacetylated substrate or standard.
Applications:
This assay is a two-step procedure in a microtiter plate. Samples are incubated with the HDAC assay substrate, allowing deacetylation of the substrate. Next, the Activator Solution releases p-nitroanilide from the deacetylated substrate or standard.
Key Applications:
Enzyme Assay
Usage Statement:
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Entrez Gene Summary:
Histones play a critical role in transcriptional regulation, cell cycle progression, and developmental events. Histone acetylation/deacetylation alters chromosome structure and affects transcription factor access to DNA. The protein encoded by this gene belongs to the class II histone deacetylase/acuc/apha family. It possesses histone deacetylase activity and represses transcription when tethered to a promoter. It coimmunoprecipitates only with HDAC3 family member and might form multicomplex proteins. It also interacts with myocyte enhancer factor-2 (MEF2) proteins, resulting in repression of MEF2-dependent genes. This gene is thought to be associated with colon cancer. Two transcript variants encoding different isoforms have been found for this gene.
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UniProt Summary:
FUNCTION: SwissProt: Q9UQL6 # Responsible for the deacetylation of lysine residues on the N-terminal part of the core histones (H2A, H2B, H3 and H4). Histone deacetylation gives a tag for epigenetic repression and plays an important role in transcriptional regulation, cell cycle progression and developmental events. Histone deacetylases act via the formation of large multiprotein complexes. Involved in muscle maturation by repressing transcription of myocyte enhancer MEF2C. During muscle differentiation, it shuttles into the cytoplasm, allowing the expression of myocyte enhancer factors.
SIZE: 1122 amino acids; 121992 Da
SUBUNIT: Interacts with BCOR, HDAC7, HDAC9, CTBP1, MEF2C, NCOR2, NRIP1, PHB2 and a 14-3-3 chaperone protein. Interacts with JARID1B.
SUBCELLULAR LOCATION: Nucleus. Cytoplasm. Note=Shuttles between the nucleus and the cytoplasm. In muscle cells, it shuttles into the cytoplasm during myocyte differentiation. The export to cytoplasm depends on the interaction with a 14-3-3 chaperone protein and is due to its phosphorylation at Ser-259 and Ser-498 by CaMK.TISSUE SPECIFICITY: Ubiquitous.DOMAIN:SwissProt: Q9UQL6 The nuclear export sequence mediates the shuttling between the nucleus and the cytoplasm (By similarity).
PTM: Phosphorylated by CaMK at Ser-259 and Ser-498. The phosphorylation is RT

10058   Amplite Colorimetric Urea Quantitation Kit *Blue Color*   品牌 AAT

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Amplite Colorimetric Urea Quantitation Kit *Blue Color*

货号： 10058      产品名称： Amplite Colorimetric Urea Quantitation Kit *Blue Color*   品牌： AAT 规格： 1 kit 二周到货 生化试验

Amplite� Colorimetric Urea Quantitation Kit *Blue Color*
Cat # 10058
Storage F/D/L (see storage codes below)
Unit Size 200 Tests
Ex (nm) 650 
Urea is the final degradation product of protein and amino acid metabolism in animals. It is produced in liver, secreted by kidney and excreted through urine. The determination of urea is very useful test in clinical laboratory to monitor health status. The Blood Urea Nitrogen (BUN) test is a measure of the amount of nitrogen in the blood in the form of urea and is primarily used, along with the creatinine test to evaluate kidney function, helping diagnose kidney diseases. Our Amplite� Colorimetric Urea Assay Kit provides a simple and sensitive colorimetric method for the quantitation of urea concentration in biological samples such as serum, plasma and urine, etc. The assay is based on an enzyme-coupled reaction of urea in the assay buffer, and finally produces a blue colored product. The intensity of color produced is proportional to the concentration of urea in the sample, which can be measured colorimetrically at 660-670 nm. This Amplite� Colorimetric Urea Assay Kit provides a simple assay to detect as little as 10 礛 urea in a 150 礚 assay volume. The assay can be performed in a convenient 96-well or 384-well microtiter-plate format and easily adapted to automation without a separation step.  
Detailed Information:   Protocol      References      MSDS
Related Products:     Diagnostic Molecules     Cell Metabolism 
Storage Codes:
  D = Desiccated
  L = Avoid light
  F = Freeze (<-15 癈)
  R = Refrigerated (2-4 癈)
  RT = Room temperature (4-50 癈)

 

 

 

 

13801   Amplite Colorimetric Aspartate Aminotransferase (AST) Assay Kit   品牌 AAT

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Amplite Colorimetric Aspartate Aminotransferase (AST) Assay Kit

货号： 13801      产品名称： Amplite Colorimetric Aspartate Aminotransferase (AST) Assay Kit   品牌： AAT 规格： 1 kit 二周到货 生化试验

Amplite� Colorimetric Aspartate Aminotransferase (AST) Assay Kit 
Cat # 13801
Storage F/D/L (see storage codes below)
Unit Size 200 Tests
Ex (nm) 575 
Aspartate aminotransferase (AST), also called serum glutamic oxaloacetic transaminase (GOT), is a member of transferase family. It catalyzes the reversible transfer of an alpha-amino group between aspartate and glutamate, and is an important enzyme in amino acid metabolism. AST is found in many body tissues such as liver, heart, muscle, kidneys, brain. In healthy subjects, serum AST levels are low. However, when cells are damaged, such as acute and chronic hepatitis, obstructive jaundice, carcinoma of liver, myocardial infarction, AST may leak into the blood stream and the AST levels are significantly elevated. Therefore, determination of serum AST level has great clinical and diagnostic significance. Amplite� Colorimetric Aspartate Aminotransferase (AST) assay kit provides a quick and sensitive method for the measurement of AST in various biological samples. Aspartate transaminase catalyzes the reaction of aspartate and _-ketoglutarate to oxaloacetate and glutamate. The product L-glutamate is measured by the generation of a blue color product through an enzyme coupled reaction cycle. The signal can be read by an absorbance microplate reader at ~575 nm or by the absorbance ratio of A570nm to A610nm to increase assay sensitivity. With the Amplite� Colorimetric Aspartate Aminotransferase Assay Kit, we have detected as little as 2 mU/mL AST in a 100 礚 reaction volume. The assay is robust, and can be readily adapted for a wide variety of applications. 
Detailed Information:   Protocol      References      MSDS
Related Products:     Transferases 
Storage Codes:
  D = Desiccated
  L = Avoid light
  F = Freeze (<-15 癈)
  R = Refrigerated (2-4 癈)
  RT = Room temperature (4-50 癈)

 

 

13803   Amplite Colorimetric Alanine Aminotransferase Assay Kit *Blue Color*   品牌 AAT

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Amplite Colorimetric Alanine Aminotransferase Assay Kit *Blue Color*

货号： 13803      产品名称： Amplite Colorimetric Alanine Aminotransferase Assay Kit *Blue Color*   品牌： AAT 规格： 1 kit 二周到货 生化试验

Amplite� Colorimetric Alanine Aminotransferase Assay Kit *Blue Color*
Cat # 13803
Storage F/D/L (see storage codes below)
Unit Size 200 Tests
Ex (nm) 575
Alanine aminotransferase (ALT), also called serum glutamate pyruvic transaminase (GPT), is a member of transferase family. It catalyzes the reversible transfer of an alpha-amino group between alanine and glutamate, and is an important enzyme in amino acid metabolism. ALT is found mainly in liver and small amount in heart, muscle, and kidneys. In healthy subjects, serum ALT levels are low. However, when cells are damaged, such as acute and chronic hepatitis, obstructive jaundice, carcinoma of liver, myocardial infarction, ALT may leak into the blood stream and the ALT levels are significantly elevated. Therefore, determination of serum ALT level has great clinical and diagnostic significance. Amplite� Colorimetric Alanine Aminotransferase Assay Kit provides a quick and sensitive method for the measurement of ALT in various biological samples. ALT catalyzes the reaction of alanine and _-ketoglutarate to pyruvate and glutamate. The product glutamate is measured by the generation of a blue color product through an enzyme coupled reaction cycle. The signal can be read by an absorbance microplate reader at ~575 nm or by the absorbance ratio of A570nm to A610nm to increase assay sensitivity. With the Amplite� Colorimetric Alanine Aminotransferase Assay Kit as little as 10 mU/mL ALT was detected in a 100 礚 reaction volume. The assay is robust, and can be readily adapted for a wide variety of applications.
Detailed Information:   Protocol      References      MSDS
Related Products:     Transferases 
Storage Codes:
  D = Desiccated
  L = Avoid light
  F = Freeze (<-15 癈)
  R = Refrigerated (2-4 癈)
  RT = Room temperature (4-50 癈)