Coelenterazine BioChemica
货号: A2817_0001 产品名称: Coelenterazine BioChemica 品牌: AppliChem 规格: 1mg
A7508_0001 Coelenterazine H BioChemica 品牌 AppliChem
Coelenterazine H BioChemica
货号: A7508_0001 产品名称: Coelenterazine H BioChemica 品牌: AppliChem 规格: 1mg
S2011 Coelenterazine-h 品牌 Promega
Coelenterazine-h
货号: S2011 产品名称: Coelenterazine-h 品牌: Promega 规格: 250μg 二周到货 生化试验
说明
来自海肾(Renilla )、Aequorea 和其它海洋生物的萤光素酶,通常用作真核细胞表达分析的细胞现象的指示剂或报告蛋白。海肾萤光素酶经常被用作转录调控的报告子,而apoaequorin 经常被用作钙指示剂。Coelenterazine ( 腔肠素) 的其它应用还包括在细胞或组织中用化学发光法检测Reactive Oxygen Species (ROS)。Promega还提供多种腔肠素类似物,包括:
Coelenterazine(native):海肾萤光素酶和apoaequorin 的发光底物。 分子式:C26H21N3O3。分子量:423.5。形态:冻干薄膜状。
Coelenterazine-h:是一种水母发光蛋白复合物,其发光强度比野生型腔肠素高10-20 倍,是检测Ca2+ 浓度细微变化的有力工具。分子式:C26H21N3O2。分子量:407.5。形态:冻干薄膜状。
特点 • 高纯度:≥ 95%。
• 可定制:可按客户要求包装。
• 易制备:提供的是干燥底物,易于在甲醇或乙醇中制备。
C397 (luminescent)钙探针 Coelenterazine-WS 品牌 Japan
(luminescent)钙探针 Coelenterazine-WS
货号: C397 产品名称: (luminescent)钙探针 Coelenterazine-WS 品牌: Japan 规格: 1 mg
ENZ-52054 Coelenterazine (Ultra Pure) 品牌 Enzo
Coelenterazine (Ultra Pure)
货号: ENZ-52054 产品名称: Coelenterazine (Ultra Pure) 品牌: Enzo 规格: 250 ug
CZ10 腔肠素 Coelenterazine 品牌 Goldbio
腔肠素 Coelenterazine
货号: CZ10 产品名称: 腔肠素 Coelenterazine 品牌: Goldbio 规格: 1MG
CZ5 腔肠素 Coelenterazine 品牌 Goldbio
腔肠素 Coelenterazine
货号: CZ5 产品名称: 腔肠素 Coelenterazine 品牌: Goldbio 规格: 5MG
21150 Coelenterazine *UltraPure grade* 品牌 AAT
Coelenterazine *UltraPure grade*
货号: 21150 产品名称: Coelenterazine *UltraPure grade* 品牌: AAT 规格: 250 ug 二周到货 生化试验
Coelenterazine *UltraPure grade*
Cat # 21150
Storage F/D/L (see storage codes below)
Unit Size 250 ug
Ex (nm) 429
Em (nm) 466
MW 423.46
Solvent Ethanol
Calcium measurement is critical for numerous biological investigations. The aequorin complex comprises a 22 kD apoaequorin protein, molecular oxygen and the luminophore coelenterazine. When three Ca2+ ions bind to this complex, coelenterazine is oxidized to coelenteramide, with a concomitant release of carbon dioxide and blue light. The approximately third-power dependence of aequorin’s bioluminescence on Ca2+ concentration allows the measurement of Ca2+ concentrations with a broad detection range from ~0.1 礛 to >100 礛. Unlike fluorescent Ca2+ indicators, Ca2+-bound aequorin can be detected without illuminating the sample, thereby eliminating interference from autofluorescence. Native coelenterazine and its derivatives confer different Ca2+ affinities and spectral properties on the aequorin complex. Recombinant apoaequorin reconstituted with coelenterazine hcp is reported to have the best luminescence overall, with both a high quantum yield and a fast response time. However, intracellular reconstitution of aequorin from coelenterazine analogs can be relatively slow. Aequorins containing the cp, f or h form of coelenterazine exhibit 10�20 times stronger luminescence than that of apoaequorin reconstituted with native coelenterazine. Coelenterazines h and cp has been used in HTS screening assay for GPCRs.
Detailed Information: Protocol References MSDS
Related Products: Calcium GPCR Assays Calcium Channels pH and Ion Indicators
Storage Codes:
D = Desiccated
L = Avoid light
F = Freeze (<-15 癈)
R = Refrigerated (2-4 癈)
RT = Room temperature (4-50 癈)
21151 Coelenterazine cp *UltraPure grade* 品牌 AAT
Coelenterazine cp *UltraPure grade*
货号: 21151 产品名称: Coelenterazine cp *UltraPure grade* 品牌: AAT 规格: 250 ug 二周到货 生化试验
Coelenterazine cp *UltraPure grade*
Cat # 21151
Storage F/D/L (see storage codes below)
Unit Size 250 ug
Ex (nm) 430
Em (nm) 442
MW 415.48
Solvent Ethanol
Calcium measurement is critical for numerous biological investigations. The aequorin complex comprises a 22 kD apoaequorin protein, molecular oxygen and the luminophore coelenterazine. When three Ca2+ ions bind to this complex, coelenterazine is oxidized to coelenteramide, with a concomitant release of carbon dioxide and blue light. The approximately third-power dependence of aequorin’s bioluminescence on Ca2+ concentration allows the measurement of Ca2+ concentrations with a broad detection range from ~0.1 礛 to >100 礛. Unlike fluorescent Ca2+ indicators, Ca2+-bound aequorin can be detected without illuminating the sample, thereby eliminating interference from autofluorescence. Native coelenterazine and its derivatives confer different Ca2+ affinities and spectral properties on the aequorin complex. Recombinant apoaequorin reconstituted with coelenterazine hcp is reported to have the best luminescence overall, with both a high quantum yield and a fast response time. However, intracellular reconstitution of aequorin from coelenterazine analogs can be relatively slow. Aequorins containing the cp, f or h form of coelenterazine exhibit 10�20 times stronger luminescence than that of apoaequorin reconstituted with native coelenterazine. Coelenterazines h and cp has been used in HTS screening assay for GPCRs.
Detailed Information: Protocol References MSDS
Related Products: Calcium GPCR Assays Calcium Channels pH and Ion Indicators
Storage Codes:
D = Desiccated
L = Avoid light
F = Freeze (<-15 癈)
R = Refrigerated (2-4 癈)
RT = Room temperature (4-50 癈)
21152 Coelenterazine f *UltraPure grade* 品牌 AAT
Coelenterazine f *UltraPure grade*
货号: 21152 产品名称: Coelenterazine f *UltraPure grade* 品牌: AAT 规格: 250 ug 二周到货 生化试验
Coelenterazine f *UltraPure grade*
Cat # 21152
Storage F/D/L (see storage codes below)
Unit Size 250 ug
Ex (nm) 437
Em (nm) 472
MW 425.45
Solvent Ethanol
Calcium measurement is critical for numerous biological investigations. The aequorin complex comprises a 22 kD apoaequorin protein, molecular oxygen and the luminophore coelenterazine. When three Ca2+ ions bind to this complex, coelenterazine is oxidized to coelenteramide, with a concomitant release of carbon dioxide and blue light. The approximately third-power dependence of aequorin’s bioluminescence on Ca2+ concentration allows the measurement of Ca2+ concentrations with a broad detection range from ~0.1 礛 to >100 礛. Unlike fluorescent Ca2+ indicators, Ca2+-bound aequorin can be detected without illuminating the sample, thereby eliminating interference from autofluorescence. Native coelenterazine and its derivatives confer different Ca2+ affinities and spectral properties on the aequorin complex. Recombinant apoaequorin reconstituted with coelenterazine hcp is reported to have the best luminescence overall, with both a high quantum yield and a fast response time. However, intracellular reconstitution of aequorin from coelenterazine analogs can be relatively slow. Aequorins containing the cp, f or h form of coelenterazine exhibit 10�20 times stronger luminescence than that of apoaequorin reconstituted with native coelenterazine. Coelenterazines h and cp has been used in HTS screening assay for GPCRs.
Detailed Information: Protocol References MSDS
Related Products: Calcium GPCR Assays Calcium Channels pH and Ion Indicators
Storage Codes:
D = Desiccated
L = Avoid light
F = Freeze (<-15 癈)
R = Refrigerated (2-4 癈)
RT = Room temperature (4-50 癈)
21153 Coelenterazine h *UltraPure grade* 品牌 AAT
Coelenterazine h *UltraPure grade*
货号: 21153 产品名称: Coelenterazine h *UltraPure grade* 品牌: AAT 规格: 250 ug 二周到货 生化试验
Coelenterazine h *UltraPure grade*
Cat # 21153
Storage F/D/L (see storage codes below)
Unit Size 250 ug
Ex (nm) 437
Em (nm) 466
MW 407.46
Solvent Ethanol
Calcium measurement is critical for numerous biological investigations. The aequorin complex comprises a 22 kD apoaequorin protein, molecular oxygen and the luminophore coelenterazine. When three Ca2+ ions bind to this complex, coelenterazine is oxidized to coelenteramide, with a concomitant release of carbon dioxide and blue light. The approximately third-power dependence of aequorin’s bioluminescence on Ca2+ concentration allows the measurement of Ca2+ concentrations with a broad detection range from ~0.1 礛 to >100 礛. Unlike fluorescent Ca2+ indicators, Ca2+-bound aequorin can be detected without illuminating the sample, thereby eliminating interference from autofluorescence. Native coelenterazine and its derivatives confer different Ca2+ affinities and spectral properties on the aequorin complex. Recombinant apoaequorin reconstituted with coelenterazine hcp is reported to have the best luminescence overall, with both a high quantum yield and a fast response time. However, intracellular reconstitution of aequorin from coelenterazine analogs can be relatively slow. Aequorins containing the cp, f or h form of coelenterazine exhibit 10�20 times stronger luminescence than that of apoaequorin reconstituted with native coelenterazine. Coelenterazines h and cp has been used in HTS screening assay for GPCRs.
Detailed Information: Protocol References MSDS
Related Products: Calcium GPCR Assays Calcium Channels pH and Ion Indicators
Storage Codes:
D = Desiccated
L = Avoid light
F = Freeze (<-15 癈)
R = Refrigerated (2-4 癈)
RT = Room temperature (4-50 癈)
21154 Coelenterazine hcp *UltraPure grade* 品牌 AAT
Coelenterazine hcp *UltraPure grade*
货号: 21154 产品名称: Coelenterazine hcp *UltraPure grade* 品牌: AAT 规格: 250 ug 二周到货 生化试验
Coelenterazine hcp *UltraPure grade*
Cat # 21154
Storage F/D/L (see storage codes below)
Unit Size 250 ug
Ex (nm) 433
Em (nm) 445
MW 399.48
Solvent Ethanol
Calcium measurement is critical for numerous biological investigations. The aequorin complex comprises a 22 kD apoaequorin protein, molecular oxygen and the luminophore coelenterazine. When three Ca2+ ions bind to this complex, coelenterazine is oxidized to coelenteramide, with a concomitant release of carbon dioxide and blue light. The approximately third-power dependence of aequorin’s bioluminescence on Ca2+ concentration allows the measurement of Ca2+ concentrations with a broad detection range from ~0.1 礛 to >100 礛. Unlike fluorescent Ca2+ indicators, Ca2+-bound aequorin can be detected without illuminating the sample, thereby eliminating interference from autofluorescence. Native coelenterazine and its derivatives confer different Ca2+ affinities and spectral properties on the aequorin complex. Recombinant apoaequorin reconstituted with coelenterazine hcp is reported to have the best luminescence overall, with both a high quantum yield and a fast response time. However, intracellular reconstitution of aequorin from coelenterazine analogs can be relatively slow. Aequorins containing the cp, f or h form of coelenterazine exhibit 10�20 times stronger luminescence than that of apoaequorin reconstituted with native coelenterazine. Coelenterazines h and cp has been used in HTS screening assay for GPCRs.
Detailed Information: Protocol References MSDS
Related Products: Calcium GPCR Assays Calcium Channels pH and Ion Indicators
Storage Codes:
D = Desiccated
L = Avoid light
F = Freeze (<-15 癈)
R = Refrigerated (2-4 癈)
RT = Room temperature (4-50 癈)
21155 Coelenterazine n *UltraPure grade* 品牌 AAT
Coelenterazine n *UltraPure grade*
货号: 21155 产品名称: Coelenterazine n *UltraPure grade* 品牌: AAT 规格: 250 ug 二周到货 生化试验
Coelenterazine n *UltraPure grade*
Cat # 21155
Storage F/D/L (see storage codes below)
Unit Size 250 ug
Ex (nm) 431
Em (nm) 468
MW 457.52
Solvent Ethanol
Calcium measurement is critical for numerous biological investigations. The aequorin complex comprises a 22 kD apoaequorin protein, molecular oxygen and the luminophore coelenterazine. When three Ca2+ ions bind to this complex, coelenterazine is oxidized to coelenteramide, with a concomitant release of carbon dioxide and blue light. The approximately third-power dependence of aequorin’s bioluminescence on Ca2+ concentration allows the measurement of Ca2+ concentrations with a broad detection range from ~0.1 礛 to >100 礛. Unlike fluorescent Ca2+ indicators, Ca2+-bound aequorin can be detected without illuminating the sample, thereby eliminating interference from autofluorescence. Native coelenterazine and its derivatives confer different Ca2+ affinities and spectral properties on the aequorin complex. Recombinant apoaequorin reconstituted with coelenterazine hcp is reported to have the best luminescence overall, with both a high quantum yield and a fast response time. However, intracellular reconstitution of aequorin from coelenterazine analogs can be relatively slow. Aequorins containing the cp, f or h form of coelenterazine exhibit 10�20 times stronger luminescence than that of apoaequorin reconstituted with native coelenterazine. Coelenterazines h and cp has been used in HTS screening assay for GPCRs.
Detailed Information: Protocol References MSDS
Related Products: Calcium GPCR Assays Calcium Channels pH and Ion Indicators
Storage Codes:
D = Desiccated
L = Avoid light
F = Freeze (<-15 癈)
R = Refrigerated (2-4 癈)
RT = Room temperature (4-50 癈)
21156 Coelenterazine *UltraPure grade* 品牌 AAT
Coelenterazine *UltraPure grade*
货号: 21156 产品名称: Coelenterazine *UltraPure grade* 品牌: AAT 规格: 1 mg 二周到货 生化试验
Coelenterazine *UltraPure grade*
Cat # 21156
Storage F/D/L (see storage codes below)
Unit Size 1 mg
Ex (nm) 429
Em (nm) 466
MW 423.46
Solvent Ethanol
Calcium measurement is critical for numerous biological investigations. The aequorin complex comprises a 22 kD apoaequorin protein, molecular oxygen and the luminophore coelenterazine. When three Ca2+ ions bind to this complex, coelenterazine is oxidized to coelenteramide, with a concomitant release of carbon dioxide and blue light. The approximately third-power dependence of aequorin’s bioluminescence on Ca2+ concentration allows the measurement of Ca2+ concentrations with a broad detection range from ~0.1 礛 to >100 礛. Unlike fluorescent Ca2+ indicators, Ca2+-bound aequorin can be detected without illuminating the sample, thereby eliminating interference from autofluorescence. Native coelenterazine and its derivatives confer different Ca2+ affinities and spectral properties on the aequorin complex. Recombinant apoaequorin reconstituted with coelenterazine hcp is reported to have the best luminescence overall, with both a high quantum yield and a fast response time. However, intracellular reconstitution of aequorin from coelenterazine analogs can be relatively slow. Aequorins containing the cp, f or h form of coelenterazine exhibit 10�20 times stronger luminescence than that of apoaequorin reconstituted with native coelenterazine. Coelenterazines h and cp has been used in HTS screening assay for GPCRs.
Detailed Information: Protocol References MSDS
Related Products: Calcium GPCR Assays Calcium Channels pH and Ion Indicators
Storage Codes:
D = Desiccated
L = Avoid light
F = Freeze (<-15 癈)
R = Refrigerated (2-4 癈)
RT = Room temperature (4-50 癈)
21157 Coelenterazine cp *UltraPure grade* 品牌 AAT
Coelenterazine cp *UltraPure grade*
货号: 21157 产品名称: Coelenterazine cp *UltraPure grade* 品牌: AAT 规格: 1 mg 二周到货 生化试验
Coelenterazine cp *UltraPure grade*
Cat # 21157
Storage F/D/L (see storage codes below)
Unit Size 1 mg
Ex (nm) 430
Em (nm) 442
MW 415.48
Solvent Ethanol
Calcium measurement is critical for numerous biological investigations. The aequorin complex comprises a 22 kD apoaequorin protein, molecular oxygen and the luminophore coelenterazine. When three Ca2+ ions bind to this complex, coelenterazine is oxidized to coelenteramide, with a concomitant release of carbon dioxide and blue light. The approximately third-power dependence of aequorin’s bioluminescence on Ca2+ concentration allows the measurement of Ca2+ concentrations with a broad detection range from ~0.1 礛 to >100 礛. Unlike fluorescent Ca2+ indicators, Ca2+-bound aequorin can be detected without illuminating the sample, thereby eliminating interference from autofluorescence. Native coelenterazine and its derivatives confer different Ca2+ affinities and spectral properties on the aequorin complex. Recombinant apoaequorin reconstituted with coelenterazine hcp is reported to have the best luminescence overall, with both a high quantum yield and a fast response time. However, intracellular reconstitution of aequorin from coelenterazine analogs can be relatively slow. Aequorins containing the cp, f or h form of coelenterazine exhibit 10�20 times stronger luminescence than that of apoaequorin reconstituted with native coelenterazine. Coelenterazines h and cp has been used in HTS screening assay for GPCRs.
Detailed Information: Protocol References MSDS
Related Products: Calcium GPCR Assays Calcium Channels pH and Ion Indicators
Storage Codes:
D = Desiccated
L = Avoid light
F = Freeze (<-15 癈)
R = Refrigerated (2-4 癈)
RT = Room temperature (4-50 癈)
21158 Coelenterazine f *UltraPure grade* 品牌 AAT
Coelenterazine f *UltraPure grade*
货号: 21158 产品名称: Coelenterazine f *UltraPure grade* 品牌: AAT 规格: 1 mg 二周到货 生化试验
Coelenterazine f *UltraPure grade*
Cat # 21158
Storage F/D/L (see storage codes below)
Unit Size 1 mg
Ex (nm) 437
Em (nm) 472
MW 425.45
Solvent Ethanol
Calcium measurement is critical for numerous biological investigations. The aequorin complex comprises a 22 kD apoaequorin protein, molecular oxygen and the luminophore coelenterazine. When three Ca2+ ions bind to this complex, coelenterazine is oxidized to coelenteramide, with a concomitant release of carbon dioxide and blue light. The approximately third-power dependence of aequorin’s bioluminescence on Ca2+ concentration allows the measurement of Ca2+ concentrations with a broad detection range from ~0.1 礛 to >100 礛. Unlike fluorescent Ca2+ indicators, Ca2+-bound aequorin can be detected without illuminating the sample, thereby eliminating interference from autofluorescence. Native coelenterazine and its derivatives confer different Ca2+ affinities and spectral properties on the aequorin complex. Recombinant apoaequorin reconstituted with coelenterazine hcp is reported to have the best luminescence overall, with both a high quantum yield and a fast response time. However, intracellular reconstitution of aequorin from coelenterazine analogs can be relatively slow. Aequorins containing the cp, f or h form of coelenterazine exhibit 10�20 times stronger luminescence than that of apoaequorin reconstituted with native coelenterazine. Coelenterazines h and cp has been used in HTS screening assay for GPCRs.
Detailed Information: Protocol References MSDS
Related Products: Calcium GPCR Assays Calcium Channels pH and Ion Indicators
Storage Codes:
D = Desiccated
L = Avoid light
F = Freeze (<-15 癈)
R = Refrigerated (2-4 癈)
RT = Room temperature (4-50 癈)
21159 Coelenterazine h *UltraPure grade* 品牌 AAT
Coelenterazine h *UltraPure grade*
货号: 21159 产品名称: Coelenterazine h *UltraPure grade* 品牌: AAT 规格: 1 mg 二周到货 生化试验
Coelenterazine h *UltraPure grade*
Cat # 21159
Storage F/D/L (see storage codes below)
Unit Size 1 mg
Ex (nm) 437
Em (nm) 466
MW 407.46
Solvent Ethanol
Calcium measurement is critical for numerous biological investigations. The aequorin complex comprises a 22 kD apoaequorin protein, molecular oxygen and the luminophore coelenterazine. When three Ca2+ ions bind to this complex, coelenterazine is oxidized to coelenteramide, with a concomitant release of carbon dioxide and blue light. The approximately third-power dependence of aequorin’s bioluminescence on Ca2+ concentration allows the measurement of Ca2+ concentrations with a broad detection range from ~0.1 礛 to >100 礛. Unlike fluorescent Ca2+ indicators, Ca2+-bound aequorin can be detected without illuminating the sample, thereby eliminating interference from autofluorescence. Native coelenterazine and its derivatives confer different Ca2+ affinities and spectral properties on the aequorin complex. Recombinant apoaequorin reconstituted with coelenterazine hcp is reported to have the best luminescence overall, with both a high quantum yield and a fast response time. However, intracellular reconstitution of aequorin from coelenterazine analogs can be relatively slow. Aequorins containing the cp, f or h form of coelenterazine exhibit 10�20 times stronger luminescence than that of apoaequorin reconstituted with native coelenterazine. Coelenterazines h and cp has been used in HTS screening assay for GPCRs.
Detailed Information: Protocol References MSDS
Related Products: Calcium GPCR Assays Calcium Channels pH and Ion Indicators
Storage Codes:
D = Desiccated
L = Avoid light
F = Freeze (<-15 癈)
R = Refrigerated (2-4 癈)
RT = Room temperature (4-50 癈)
21160 Coelenterazine hcp *UltraPure grade* 品牌 AAT
Coelenterazine hcp *UltraPure grade*
货号: 21160 产品名称: Coelenterazine hcp *UltraPure grade* 品牌: AAT 规格: 1 mg 二周到货 生化试验
Coelenterazine hcp *UltraPure grade*
Cat # 21160
Storage F/D/L (see storage codes below)
Unit Size 1 mg
Ex (nm) 433
Em (nm) 445
MW 399.48
Solvent Ethanol
Calcium measurement is critical for numerous biological investigations. The aequorin complex comprises a 22 kD apoaequorin protein, molecular oxygen and the luminophore coelenterazine. When three Ca2+ ions bind to this complex, coelenterazine is oxidized to coelenteramide, with a concomitant release of carbon dioxide and blue light. The approximately third-power dependence of aequorin’s bioluminescence on Ca2+ concentration allows the measurement of Ca2+ concentrations with a broad detection range from ~0.1 礛 to >100 礛. Unlike fluorescent Ca2+ indicators, Ca2+-bound aequorin can be detected without illuminating the sample, thereby eliminating interference from autofluorescence. Native coelenterazine and its derivatives confer different Ca2+ affinities and spectral properties on the aequorin complex. Recombinant apoaequorin reconstituted with coelenterazine hcp is reported to have the best luminescence overall, with both a high quantum yield and a fast response time. However, intracellular reconstitution of aequorin from coelenterazine analogs can be relatively slow. Aequorins containing the cp, f or h form of coelenterazine exhibit 10�20 times stronger luminescence than that of apoaequorin reconstituted with native coelenterazine. Coelenterazines h and cp has been used in HTS screening assay for GPCRs.
Detailed Information: Protocol References MSDS
Related Products: Calcium GPCR Assays Calcium Channels pH and Ion Indicators
Storage Codes:
D = Desiccated
L = Avoid light
F = Freeze (<-15 癈)
R = Refrigerated (2-4 癈)
RT = Room temperature (4-50 癈)
21161 Coelenterazine n *UltraPure grade* 品牌 AAT
Coelenterazine n *UltraPure grade*
货号: 21161 产品名称: Coelenterazine n *UltraPure grade* 品牌: AAT 规格: 1 mg 二周到货 生化试验
Coelenterazine n *UltraPure grade*
Cat # 21161
Storage F/D/L (see storage codes below)
Unit Size 1 mg
Ex (nm) 431
Em (nm) 468
MW 457.52
Solvent Ethanol
Calcium measurement is critical for numerous biological investigations. The aequorin complex comprises a 22 kD apoaequorin protein, molecular oxygen and the luminophore coelenterazine. When three Ca2+ ions bind to this complex, coelenterazine is oxidized to coelenteramide, with a concomitant release of carbon dioxide and blue light. The approximately third-power dependence of aequorin’s bioluminescence on Ca2+ concentration allows the measurement of Ca2+ concentrations with a broad detection range from ~0.1 礛 to >100 礛. Unlike fluorescent Ca2+ indicators, Ca2+-bound aequorin can be detected without illuminating the sample, thereby eliminating interference from autofluorescence. Native coelenterazine and its derivatives confer different Ca2+ affinities and spectral properties on the aequorin complex. Recombinant apoaequorin reconstituted with coelenterazine hcp is reported to have the best luminescence overall, with both a high quantum yield and a fast response time. However, intracellular reconstitution of aequorin from coelenterazine analogs can be relatively slow. Aequorins containing the cp, f or h form of coelenterazine exhibit 10�20 times stronger luminescence than that of apoaequorin reconstituted with native coelenterazine. Coelenterazines h and cp has been used in HTS screening assay for GPCRs.
Detailed Information: Protocol References MSDS
Related Products: Calcium GPCR Assays Calcium Channels pH and Ion Indicators
Storage Codes:
D = Desiccated
L = Avoid light
F = Freeze (<-15 癈)
R = Refrigerated (2-4 癈)
RT = Room temperature (4-50 癈)
21165 Coelenterazine h *UltraPure grade* 品牌 AAT
Coelenterazine h *UltraPure grade*
货号: 21165 产品名称: Coelenterazine h *UltraPure grade* 品牌: AAT 规格: 10 mg 二周到货 生化试验
Coelenterazine h *UltraPure grade*
Cat # 21165
Storage F/D/L (see storage codes below)
Unit Size 10 mg
Ex (nm) 437
Em (nm) 466
MW 407.46
Solvent Ethanol
Calcium measurement is critical for numerous biological investigations. The aequorin complex comprises a 22 kD apoaequorin protein, molecular oxygen and the luminophore coelenterazine. When three Ca2+ ions bind to this complex, coelenterazine is oxidized to coelenteramide, with a concomitant release of carbon dioxide and blue light. The approximately third-power dependence of aequorin’s bioluminescence on Ca2+ concentration allows the measurement of Ca2+ concentrations with a broad detection range from ~0.1 礛 to >100 礛. Unlike fluorescent Ca2+ indicators, Ca2+-bound aequorin can be detected without illuminating the sample, thereby eliminating interference from autofluorescence. Native coelenterazine and its derivatives confer different Ca2+ affinities and spectral properties on the aequorin complex. Recombinant apoaequorin reconstituted with coelenterazine hcp is reported to have the best luminescence overall, with both a high quantum yield and a fast response time. However, intracellular reconstitution of aequorin from coelenterazine analogs can be relatively slow. Aequorins containing the cp, f or h form of coelenterazine exhibit 10�20 times stronger luminescence than that of apoaequorin reconstituted with native coelenterazine. Coelenterazines h and cp has been used in HTS screening assay for GPCRs.
Detailed Information: Protocol References MSDS
Related Products: Calcium GPCR Assays Calcium Channels pH and Ion Indicators
Storage Codes:
D = Desiccated
L = Avoid light
F = Freeze (<-15 癈)
R = Refrigerated (2-4 癈)
RT = Room temperature (4-50 癈)