S2011   Coelenterazine-h   品牌 Promega

Coelenterazine-h

货号: S2011      产品名称: Coelenterazine-h   品牌: Promega 规格: 250μg 二周到货 生化试验

说明  
 来自海肾(Renilla )、Aequorea 和其它海洋生物的萤光素酶,通常用作真核细胞表达分析的细胞现象的指示剂或报告蛋白。海肾萤光素酶经常被用作转录调控的报告子,而apoaequorin 经常被用作钙指示剂。Coelenterazine ( 腔肠素) 的其它应用还包括在细胞或组织中用化学发光法检测Reactive Oxygen Species (ROS)。Promega还提供多种腔肠素类似物,包括:
Coelenterazine(native):海肾萤光素酶和apoaequorin 的发光底物。 分子式:C26H21N3O3。分子量:423.5。形态:冻干薄膜状。
Coelenterazine-h:是一种水母发光蛋白复合物,其发光强度比野生型腔肠素高10-20 倍,是检测Ca2+ 浓度细微变化的有力工具。分子式:C26H21N3O2。分子量:407.5。形态:冻干薄膜状。
特点  • 高纯度:≥ 95%。
• 可定制:可按客户要求包装。
• 易制备:提供的是干燥底物,易于在甲醇或乙醇中制备。

 

21150   Coelenterazine *UltraPure grade*   品牌 AAT

Coelenterazine *UltraPure grade*

货号: 21150      产品名称: Coelenterazine *UltraPure grade*   品牌: AAT 规格: 250 ug 二周到货 生化试验

Coelenterazine *UltraPure grade*
Cat # 21150
Storage F/D/L (see storage codes below)
Unit Size 250 ug
Ex (nm) 429
Em (nm) 466
MW 423.46
Solvent Ethanol
Calcium measurement is critical for numerous biological investigations. The aequorin complex comprises a 22 kD apoaequorin protein, molecular oxygen and the luminophore coelenterazine. When three Ca2+ ions bind to this complex, coelenterazine is oxidized to coelenteramide, with a concomitant release of carbon dioxide and blue light. The approximately third-power dependence of aequorin’s bioluminescence on Ca2+ concentration allows the measurement of Ca2+ concentrations with a broad detection range from ~0.1 礛 to >100 礛. Unlike fluorescent Ca2+ indicators, Ca2+-bound aequorin can be detected without illuminating the sample, thereby eliminating interference from autofluorescence. Native coelenterazine and its derivatives confer different Ca2+ affinities and spectral properties on the aequorin complex. Recombinant apoaequorin reconstituted with coelenterazine hcp is reported to have the best luminescence overall, with both a high quantum yield and a fast response time. However, intracellular reconstitution of aequorin from coelenterazine analogs can be relatively slow. Aequorins containing the cp, f or h form of coelenterazine exhibit 10�20 times stronger luminescence than that of apoaequorin reconstituted with native coelenterazine. Coelenterazines h and cp has been used in HTS screening assay for GPCRs. 
Detailed Information:   Protocol      References      MSDS
Related Products:     Calcium GPCR Assays     Calcium Channels     pH and Ion Indicators 
Storage Codes:
  D = Desiccated
  L = Avoid light
  F = Freeze (<-15 癈)
  R = Refrigerated (2-4 癈)
  RT = Room temperature (4-50 癈)

 

 

21151   Coelenterazine cp *UltraPure grade*   品牌 AAT

Coelenterazine cp *UltraPure grade*

货号: 21151      产品名称: Coelenterazine cp *UltraPure grade*   品牌: AAT 规格: 250 ug 二周到货 生化试验

Coelenterazine cp *UltraPure grade*
Cat # 21151
Storage F/D/L (see storage codes below)
Unit Size 250 ug
Ex (nm) 430
Em (nm) 442
MW 415.48
Solvent Ethanol
Calcium measurement is critical for numerous biological investigations. The aequorin complex comprises a 22 kD apoaequorin protein, molecular oxygen and the luminophore coelenterazine. When three Ca2+ ions bind to this complex, coelenterazine is oxidized to coelenteramide, with a concomitant release of carbon dioxide and blue light. The approximately third-power dependence of aequorin’s bioluminescence on Ca2+ concentration allows the measurement of Ca2+ concentrations with a broad detection range from ~0.1 礛 to >100 礛. Unlike fluorescent Ca2+ indicators, Ca2+-bound aequorin can be detected without illuminating the sample, thereby eliminating interference from autofluorescence. Native coelenterazine and its derivatives confer different Ca2+ affinities and spectral properties on the aequorin complex. Recombinant apoaequorin reconstituted with coelenterazine hcp is reported to have the best luminescence overall, with both a high quantum yield and a fast response time. However, intracellular reconstitution of aequorin from coelenterazine analogs can be relatively slow. Aequorins containing the cp, f or h form of coelenterazine exhibit 10�20 times stronger luminescence than that of apoaequorin reconstituted with native coelenterazine. Coelenterazines h and cp has been used in HTS screening assay for GPCRs. 
Detailed Information:   Protocol      References      MSDS
Related Products:     Calcium GPCR Assays     Calcium Channels     pH and Ion Indicators 
Storage Codes:
  D = Desiccated
  L = Avoid light
  F = Freeze (<-15 癈)
  R = Refrigerated (2-4 癈)
  RT = Room temperature (4-50 癈)

 

21152   Coelenterazine f *UltraPure grade*   品牌 AAT

Coelenterazine f *UltraPure grade*

货号: 21152      产品名称: Coelenterazine f *UltraPure grade*   品牌: AAT 规格: 250 ug 二周到货 生化试验

Coelenterazine f *UltraPure grade*
Cat # 21152
Storage F/D/L (see storage codes below)
Unit Size 250 ug
Ex (nm) 437
Em (nm) 472
MW 425.45
Solvent Ethanol
Calcium measurement is critical for numerous biological investigations. The aequorin complex comprises a 22 kD apoaequorin protein, molecular oxygen and the luminophore coelenterazine. When three Ca2+ ions bind to this complex, coelenterazine is oxidized to coelenteramide, with a concomitant release of carbon dioxide and blue light. The approximately third-power dependence of aequorin’s bioluminescence on Ca2+ concentration allows the measurement of Ca2+ concentrations with a broad detection range from ~0.1 礛 to >100 礛. Unlike fluorescent Ca2+ indicators, Ca2+-bound aequorin can be detected without illuminating the sample, thereby eliminating interference from autofluorescence. Native coelenterazine and its derivatives confer different Ca2+ affinities and spectral properties on the aequorin complex. Recombinant apoaequorin reconstituted with coelenterazine hcp is reported to have the best luminescence overall, with both a high quantum yield and a fast response time. However, intracellular reconstitution of aequorin from coelenterazine analogs can be relatively slow. Aequorins containing the cp, f or h form of coelenterazine exhibit 10�20 times stronger luminescence than that of apoaequorin reconstituted with native coelenterazine. Coelenterazines h and cp has been used in HTS screening assay for GPCRs. 
Detailed Information:   Protocol      References      MSDS
Related Products:     Calcium GPCR Assays     Calcium Channels     pH and Ion Indicators 
Storage Codes:
  D = Desiccated
  L = Avoid light
  F = Freeze (<-15 癈)
  R = Refrigerated (2-4 癈)
  RT = Room temperature (4-50 癈)

 

21153   Coelenterazine h *UltraPure grade*   品牌 AAT

Coelenterazine h *UltraPure grade*

货号: 21153      产品名称: Coelenterazine h *UltraPure grade*   品牌: AAT 规格: 250 ug 二周到货 生化试验

Coelenterazine h *UltraPure grade*
Cat # 21153
Storage F/D/L (see storage codes below)
Unit Size 250 ug
Ex (nm) 437
Em (nm) 466
MW 407.46
Solvent Ethanol
Calcium measurement is critical for numerous biological investigations. The aequorin complex comprises a 22 kD apoaequorin protein, molecular oxygen and the luminophore coelenterazine. When three Ca2+ ions bind to this complex, coelenterazine is oxidized to coelenteramide, with a concomitant release of carbon dioxide and blue light. The approximately third-power dependence of aequorin’s bioluminescence on Ca2+ concentration allows the measurement of Ca2+ concentrations with a broad detection range from ~0.1 礛 to >100 礛. Unlike fluorescent Ca2+ indicators, Ca2+-bound aequorin can be detected without illuminating the sample, thereby eliminating interference from autofluorescence. Native coelenterazine and its derivatives confer different Ca2+ affinities and spectral properties on the aequorin complex. Recombinant apoaequorin reconstituted with coelenterazine hcp is reported to have the best luminescence overall, with both a high quantum yield and a fast response time. However, intracellular reconstitution of aequorin from coelenterazine analogs can be relatively slow. Aequorins containing the cp, f or h form of coelenterazine exhibit 10�20 times stronger luminescence than that of apoaequorin reconstituted with native coelenterazine. Coelenterazines h and cp has been used in HTS screening assay for GPCRs. 
Detailed Information:   Protocol      References      MSDS
Related Products:     Calcium GPCR Assays     Calcium Channels     pH and Ion Indicators 
Storage Codes:
  D = Desiccated
  L = Avoid light
  F = Freeze (<-15 癈)
  R = Refrigerated (2-4 癈)
  RT = Room temperature (4-50 癈)

 

21154   Coelenterazine hcp *UltraPure grade*   品牌 AAT

Coelenterazine hcp *UltraPure grade*

货号: 21154      产品名称: Coelenterazine hcp *UltraPure grade*   品牌: AAT 规格: 250 ug 二周到货 生化试验

Coelenterazine hcp *UltraPure grade*
Cat # 21154
Storage F/D/L (see storage codes below)
Unit Size 250 ug
Ex (nm) 433
Em (nm) 445
MW 399.48
Solvent Ethanol
Calcium measurement is critical for numerous biological investigations. The aequorin complex comprises a 22 kD apoaequorin protein, molecular oxygen and the luminophore coelenterazine. When three Ca2+ ions bind to this complex, coelenterazine is oxidized to coelenteramide, with a concomitant release of carbon dioxide and blue light. The approximately third-power dependence of aequorin’s bioluminescence on Ca2+ concentration allows the measurement of Ca2+ concentrations with a broad detection range from ~0.1 礛 to >100 礛. Unlike fluorescent Ca2+ indicators, Ca2+-bound aequorin can be detected without illuminating the sample, thereby eliminating interference from autofluorescence. Native coelenterazine and its derivatives confer different Ca2+ affinities and spectral properties on the aequorin complex. Recombinant apoaequorin reconstituted with coelenterazine hcp is reported to have the best luminescence overall, with both a high quantum yield and a fast response time. However, intracellular reconstitution of aequorin from coelenterazine analogs can be relatively slow. Aequorins containing the cp, f or h form of coelenterazine exhibit 10�20 times stronger luminescence than that of apoaequorin reconstituted with native coelenterazine. Coelenterazines h and cp has been used in HTS screening assay for GPCRs. 
Detailed Information:   Protocol      References      MSDS
Related Products:     Calcium GPCR Assays     Calcium Channels     pH and Ion Indicators 
Storage Codes:
  D = Desiccated
  L = Avoid light
  F = Freeze (<-15 癈)
  R = Refrigerated (2-4 癈)
  RT = Room temperature (4-50 癈)

 

21155   Coelenterazine n *UltraPure grade*   品牌 AAT

Coelenterazine n *UltraPure grade*

货号: 21155      产品名称: Coelenterazine n *UltraPure grade*   品牌: AAT 规格: 250 ug 二周到货 生化试验

Coelenterazine n *UltraPure grade*
Cat # 21155
Storage F/D/L (see storage codes below)
Unit Size 250 ug
Ex (nm) 431
Em (nm) 468
MW 457.52
Solvent Ethanol
Calcium measurement is critical for numerous biological investigations. The aequorin complex comprises a 22 kD apoaequorin protein, molecular oxygen and the luminophore coelenterazine. When three Ca2+ ions bind to this complex, coelenterazine is oxidized to coelenteramide, with a concomitant release of carbon dioxide and blue light. The approximately third-power dependence of aequorin’s bioluminescence on Ca2+ concentration allows the measurement of Ca2+ concentrations with a broad detection range from ~0.1 礛 to >100 礛. Unlike fluorescent Ca2+ indicators, Ca2+-bound aequorin can be detected without illuminating the sample, thereby eliminating interference from autofluorescence. Native coelenterazine and its derivatives confer different Ca2+ affinities and spectral properties on the aequorin complex. Recombinant apoaequorin reconstituted with coelenterazine hcp is reported to have the best luminescence overall, with both a high quantum yield and a fast response time. However, intracellular reconstitution of aequorin from coelenterazine analogs can be relatively slow. Aequorins containing the cp, f or h form of coelenterazine exhibit 10�20 times stronger luminescence than that of apoaequorin reconstituted with native coelenterazine. Coelenterazines h and cp has been used in HTS screening assay for GPCRs.
Detailed Information:   Protocol      References      MSDS
Related Products:     Calcium GPCR Assays     Calcium Channels     pH and Ion Indicators 
Storage Codes:
  D = Desiccated
  L = Avoid light
  F = Freeze (<-15 癈)
  R = Refrigerated (2-4 癈)
  RT = Room temperature (4-50 癈)

 

21156   Coelenterazine *UltraPure grade*   品牌 AAT

Coelenterazine *UltraPure grade*

货号: 21156      产品名称: Coelenterazine *UltraPure grade*   品牌: AAT 规格: 1 mg 二周到货 生化试验

Coelenterazine *UltraPure grade*
Cat # 21156
Storage F/D/L (see storage codes below)
Unit Size 1 mg
Ex (nm) 429
Em (nm) 466
MW 423.46
Solvent Ethanol
Calcium measurement is critical for numerous biological investigations. The aequorin complex comprises a 22 kD apoaequorin protein, molecular oxygen and the luminophore coelenterazine. When three Ca2+ ions bind to this complex, coelenterazine is oxidized to coelenteramide, with a concomitant release of carbon dioxide and blue light. The approximately third-power dependence of aequorin’s bioluminescence on Ca2+ concentration allows the measurement of Ca2+ concentrations with a broad detection range from ~0.1 礛 to >100 礛. Unlike fluorescent Ca2+ indicators, Ca2+-bound aequorin can be detected without illuminating the sample, thereby eliminating interference from autofluorescence. Native coelenterazine and its derivatives confer different Ca2+ affinities and spectral properties on the aequorin complex. Recombinant apoaequorin reconstituted with coelenterazine hcp is reported to have the best luminescence overall, with both a high quantum yield and a fast response time. However, intracellular reconstitution of aequorin from coelenterazine analogs can be relatively slow. Aequorins containing the cp, f or h form of coelenterazine exhibit 10�20 times stronger luminescence than that of apoaequorin reconstituted with native coelenterazine. Coelenterazines h and cp has been used in HTS screening assay for GPCRs.
Detailed Information:   Protocol      References      MSDS
Related Products:     Calcium GPCR Assays     Calcium Channels     pH and Ion Indicators 
Storage Codes:
  D = Desiccated
  L = Avoid light
  F = Freeze (<-15 癈)
  R = Refrigerated (2-4 癈)
  RT = Room temperature (4-50 癈) 

21157   Coelenterazine cp *UltraPure grade*   品牌 AAT

Coelenterazine cp *UltraPure grade*

货号: 21157      产品名称: Coelenterazine cp *UltraPure grade*   品牌: AAT 规格: 1 mg 二周到货 生化试验

Coelenterazine cp *UltraPure grade*
Cat # 21157
Storage F/D/L (see storage codes below)
Unit Size 1 mg
Ex (nm) 430
Em (nm) 442
MW 415.48
Solvent Ethanol
Calcium measurement is critical for numerous biological investigations. The aequorin complex comprises a 22 kD apoaequorin protein, molecular oxygen and the luminophore coelenterazine. When three Ca2+ ions bind to this complex, coelenterazine is oxidized to coelenteramide, with a concomitant release of carbon dioxide and blue light. The approximately third-power dependence of aequorin’s bioluminescence on Ca2+ concentration allows the measurement of Ca2+ concentrations with a broad detection range from ~0.1 礛 to >100 礛. Unlike fluorescent Ca2+ indicators, Ca2+-bound aequorin can be detected without illuminating the sample, thereby eliminating interference from autofluorescence. Native coelenterazine and its derivatives confer different Ca2+ affinities and spectral properties on the aequorin complex. Recombinant apoaequorin reconstituted with coelenterazine hcp is reported to have the best luminescence overall, with both a high quantum yield and a fast response time. However, intracellular reconstitution of aequorin from coelenterazine analogs can be relatively slow. Aequorins containing the cp, f or h form of coelenterazine exhibit 10�20 times stronger luminescence than that of apoaequorin reconstituted with native coelenterazine. Coelenterazines h and cp has been used in HTS screening assay for GPCRs.
Detailed Information:   Protocol      References      MSDS
Related Products:     Calcium GPCR Assays     Calcium Channels     pH and Ion Indicators 
Storage Codes:
  D = Desiccated
  L = Avoid light
  F = Freeze (<-15 癈)
  R = Refrigerated (2-4 癈)
  RT = Room temperature (4-50 癈)
 

21158   Coelenterazine f *UltraPure grade*   品牌 AAT

Coelenterazine f *UltraPure grade*

货号: 21158      产品名称: Coelenterazine f *UltraPure grade*   品牌: AAT 规格: 1 mg 二周到货 生化试验

Coelenterazine f *UltraPure grade*
Cat # 21158
Storage F/D/L (see storage codes below)
Unit Size 1 mg
Ex (nm) 437
Em (nm) 472
MW 425.45
Solvent Ethanol
Calcium measurement is critical for numerous biological investigations. The aequorin complex comprises a 22 kD apoaequorin protein, molecular oxygen and the luminophore coelenterazine. When three Ca2+ ions bind to this complex, coelenterazine is oxidized to coelenteramide, with a concomitant release of carbon dioxide and blue light. The approximately third-power dependence of aequorin’s bioluminescence on Ca2+ concentration allows the measurement of Ca2+ concentrations with a broad detection range from ~0.1 礛 to >100 礛. Unlike fluorescent Ca2+ indicators, Ca2+-bound aequorin can be detected without illuminating the sample, thereby eliminating interference from autofluorescence. Native coelenterazine and its derivatives confer different Ca2+ affinities and spectral properties on the aequorin complex. Recombinant apoaequorin reconstituted with coelenterazine hcp is reported to have the best luminescence overall, with both a high quantum yield and a fast response time. However, intracellular reconstitution of aequorin from coelenterazine analogs can be relatively slow. Aequorins containing the cp, f or h form of coelenterazine exhibit 10�20 times stronger luminescence than that of apoaequorin reconstituted with native coelenterazine. Coelenterazines h and cp has been used in HTS screening assay for GPCRs. 
Detailed Information:   Protocol      References      MSDS
Related Products:     Calcium GPCR Assays     Calcium Channels     pH and Ion Indicators 
Storage Codes:
  D = Desiccated
  L = Avoid light
  F = Freeze (<-15 癈)
  R = Refrigerated (2-4 癈)
  RT = Room temperature (4-50 癈)

 

21159   Coelenterazine h *UltraPure grade*   品牌 AAT

Coelenterazine h *UltraPure grade*

货号: 21159      产品名称: Coelenterazine h *UltraPure grade*   品牌: AAT 规格: 1 mg 二周到货 生化试验

Coelenterazine h *UltraPure grade*
Cat # 21159
Storage F/D/L (see storage codes below)
Unit Size 1 mg
Ex (nm) 437
Em (nm) 466
MW 407.46
Solvent Ethanol
Calcium measurement is critical for numerous biological investigations. The aequorin complex comprises a 22 kD apoaequorin protein, molecular oxygen and the luminophore coelenterazine. When three Ca2+ ions bind to this complex, coelenterazine is oxidized to coelenteramide, with a concomitant release of carbon dioxide and blue light. The approximately third-power dependence of aequorin’s bioluminescence on Ca2+ concentration allows the measurement of Ca2+ concentrations with a broad detection range from ~0.1 礛 to >100 礛. Unlike fluorescent Ca2+ indicators, Ca2+-bound aequorin can be detected without illuminating the sample, thereby eliminating interference from autofluorescence. Native coelenterazine and its derivatives confer different Ca2+ affinities and spectral properties on the aequorin complex. Recombinant apoaequorin reconstituted with coelenterazine hcp is reported to have the best luminescence overall, with both a high quantum yield and a fast response time. However, intracellular reconstitution of aequorin from coelenterazine analogs can be relatively slow. Aequorins containing the cp, f or h form of coelenterazine exhibit 10�20 times stronger luminescence than that of apoaequorin reconstituted with native coelenterazine. Coelenterazines h and cp has been used in HTS screening assay for GPCRs. 
Detailed Information:   Protocol      References      MSDS
Related Products:     Calcium GPCR Assays     Calcium Channels     pH and Ion Indicators 
Storage Codes:
  D = Desiccated
  L = Avoid light
  F = Freeze (<-15 癈)
  R = Refrigerated (2-4 癈)
  RT = Room temperature (4-50 癈)

 

21160   Coelenterazine hcp *UltraPure grade*   品牌 AAT

Coelenterazine hcp *UltraPure grade*

货号: 21160      产品名称: Coelenterazine hcp *UltraPure grade*   品牌: AAT 规格: 1 mg 二周到货 生化试验

Coelenterazine hcp *UltraPure grade*
Cat # 21160
Storage F/D/L (see storage codes below)
Unit Size 1 mg
Ex (nm) 433
Em (nm) 445
MW 399.48
Solvent Ethanol
Calcium measurement is critical for numerous biological investigations. The aequorin complex comprises a 22 kD apoaequorin protein, molecular oxygen and the luminophore coelenterazine. When three Ca2+ ions bind to this complex, coelenterazine is oxidized to coelenteramide, with a concomitant release of carbon dioxide and blue light. The approximately third-power dependence of aequorin’s bioluminescence on Ca2+ concentration allows the measurement of Ca2+ concentrations with a broad detection range from ~0.1 礛 to >100 礛. Unlike fluorescent Ca2+ indicators, Ca2+-bound aequorin can be detected without illuminating the sample, thereby eliminating interference from autofluorescence. Native coelenterazine and its derivatives confer different Ca2+ affinities and spectral properties on the aequorin complex. Recombinant apoaequorin reconstituted with coelenterazine hcp is reported to have the best luminescence overall, with both a high quantum yield and a fast response time. However, intracellular reconstitution of aequorin from coelenterazine analogs can be relatively slow. Aequorins containing the cp, f or h form of coelenterazine exhibit 10�20 times stronger luminescence than that of apoaequorin reconstituted with native coelenterazine. Coelenterazines h and cp has been used in HTS screening assay for GPCRs. 
Detailed Information:   Protocol      References      MSDS
Related Products:     Calcium GPCR Assays     Calcium Channels     pH and Ion Indicators 
Storage Codes:
  D = Desiccated
  L = Avoid light
  F = Freeze (<-15 癈)
  R = Refrigerated (2-4 癈)
  RT = Room temperature (4-50 癈)

 

21161   Coelenterazine n *UltraPure grade*   品牌 AAT

Coelenterazine n *UltraPure grade*

货号: 21161      产品名称: Coelenterazine n *UltraPure grade*   品牌: AAT 规格: 1 mg 二周到货 生化试验

Coelenterazine n *UltraPure grade*
Cat # 21161
Storage F/D/L (see storage codes below)
Unit Size 1 mg
Ex (nm) 431
Em (nm) 468
MW 457.52
Solvent Ethanol
Calcium measurement is critical for numerous biological investigations. The aequorin complex comprises a 22 kD apoaequorin protein, molecular oxygen and the luminophore coelenterazine. When three Ca2+ ions bind to this complex, coelenterazine is oxidized to coelenteramide, with a concomitant release of carbon dioxide and blue light. The approximately third-power dependence of aequorin’s bioluminescence on Ca2+ concentration allows the measurement of Ca2+ concentrations with a broad detection range from ~0.1 礛 to >100 礛. Unlike fluorescent Ca2+ indicators, Ca2+-bound aequorin can be detected without illuminating the sample, thereby eliminating interference from autofluorescence. Native coelenterazine and its derivatives confer different Ca2+ affinities and spectral properties on the aequorin complex. Recombinant apoaequorin reconstituted with coelenterazine hcp is reported to have the best luminescence overall, with both a high quantum yield and a fast response time. However, intracellular reconstitution of aequorin from coelenterazine analogs can be relatively slow. Aequorins containing the cp, f or h form of coelenterazine exhibit 10�20 times stronger luminescence than that of apoaequorin reconstituted with native coelenterazine. Coelenterazines h and cp has been used in HTS screening assay for GPCRs. 
Detailed Information:   Protocol      References      MSDS
Related Products:     Calcium GPCR Assays     Calcium Channels     pH and Ion Indicators 
Storage Codes:
  D = Desiccated
  L = Avoid light
  F = Freeze (<-15 癈)
  R = Refrigerated (2-4 癈)
  RT = Room temperature (4-50 癈)

 

21165   Coelenterazine h *UltraPure grade*   品牌 AAT

Coelenterazine h *UltraPure grade*

货号: 21165      产品名称: Coelenterazine h *UltraPure grade*   品牌: AAT 规格: 10 mg 二周到货 生化试验

Coelenterazine h *UltraPure grade*
Cat # 21165
Storage F/D/L (see storage codes below)
Unit Size 10 mg
Ex (nm) 437
Em (nm) 466
MW 407.46
Solvent Ethanol
Calcium measurement is critical for numerous biological investigations. The aequorin complex comprises a 22 kD apoaequorin protein, molecular oxygen and the luminophore coelenterazine. When three Ca2+ ions bind to this complex, coelenterazine is oxidized to coelenteramide, with a concomitant release of carbon dioxide and blue light. The approximately third-power dependence of aequorin’s bioluminescence on Ca2+ concentration allows the measurement of Ca2+ concentrations with a broad detection range from ~0.1 礛 to >100 礛. Unlike fluorescent Ca2+ indicators, Ca2+-bound aequorin can be detected without illuminating the sample, thereby eliminating interference from autofluorescence. Native coelenterazine and its derivatives confer different Ca2+ affinities and spectral properties on the aequorin complex. Recombinant apoaequorin reconstituted with coelenterazine hcp is reported to have the best luminescence overall, with both a high quantum yield and a fast response time. However, intracellular reconstitution of aequorin from coelenterazine analogs can be relatively slow. Aequorins containing the cp, f or h form of coelenterazine exhibit 10�20 times stronger luminescence than that of apoaequorin reconstituted with native coelenterazine. Coelenterazines h and cp has been used in HTS screening assay for GPCRs.
Detailed Information:   Protocol      References      MSDS
Related Products:     Calcium GPCR Assays     Calcium Channels     pH and Ion Indicators 
Storage Codes:
  D = Desiccated
  L = Avoid light
  F = Freeze (<-15 癈)
  R = Refrigerated (2-4 癈)
  RT = Room temperature (4-50 癈)