标签： bp

04-321   anti-4E-BP1, rabbit mono   品牌 Millipore

anti-4E-BP1, rabbit mono

货号： 04-321      产品名称： anti-4E-BP1, rabbit mono   品牌： Millipore 规格： EA 3周到货 生化实验

Anti-4E-BP1 Antibody, rabbit monoclonal
Close
REFERENCES
The mammalian target of rapamycin (mTOR) partner, raptor, binds the mTOR substrates p70 S6 kinase and 4E-BP1 through their TOR signaling (TOS) motif
Nojima, Hiroki, et al (2003) J Biol Chem, 278:15461-4 (2003)
eIF4E binding protein 1 and H-Ras are novel substrates for the protein kinase activity of class-I phosphoinositide 3-kinase
Foukas, Lazaros C and Shepherd, Peter R (2004) Biochem Biophys Res Commun, 319:541-9 (2004)
Insulin activation of Rheb, a mediator of mTOR/S6K/4E-BP signaling, is inhibited by TSC1 and 2
Garami, Attila, et al (2003) Mol Cell, 11:1457-66 (2003)
Species Reactivity Key Applications Host Format Antibody Type
H  FC, WB, IH(P) Rabbit Purified Monoclonal Antibody
Description:
Anti-4E-BP1 Antibody, rabbit monoclonal
Promotional Text:
Special Shipping Offer on Antibodies
100% Performance Guaranteed
Trade Name:
Upstate (Millipore)
Specificity:
Recognizes the C-terminal domain of 4E-BP1.
Molecular Weight:
15-20 kDa
Immunogen:
KLH-conjugated synthetic peptide corresponding to the C-terminal region of human 4E-BP1 (eIF4E-binding protein).
Isotype:
IgG
Species Reactivity:
Human
Species Reactivity Note:
Not expected to react with mouse or rat.
Quality Assurance:
Routinely evaluated by immunoblot.
Presentation:
100 μL of rabbit monoclonal IgG in 30 mM Tris-Glycine (pH 7.4), 0.09 M NaCl, 0.006% sodium azide and 0.03% BSA and 40% glycerol.
Storage Conditions:
2 years at -20°C from date of shipment
UniProt Number:
Q13541
Entrez Gene Number:
NM_004095.3
Gene Symbol:
EIF4EBP1
PHAS-I
4EBP1
MGC4316
4E-BP1
BP-1
View All »
Alternate Names:
EIF4EBP1, eIF4E-binding protein 1, eukaryotic translation initiation factor 4E binding protein 1
Usage Statement:
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
View All »
Key Applications:
Flow Cytometry
Western Blotting
Immunohistochemistry (Paraffin)
Entrez Gene Summary:
This gene encodes one member of a family of translation repressor proteins. The protein directly interacts with eukaryotic translation initiation factor 4E (eIF4E), which is a limiting component of the multisubunit complex that recruits 40S ribosomal subunits to the 5′ end of mRNAs. Interaction of this protein with eIF4E inhibits complex assembly and represses translation. This protein is phosphorylated in response to various signals including UV irradiation and insulin signaling, resulting in its dissociation from eIF4E and activation of mRNA translation.
View All »
UniProt Summary:
FUNCTION: SwissProt: Q13541 # Regulates eIF4E activity by preventing its assembly into the eIF4F complex. Mediates the regulation of protein translation by hormones, growth factors and other stimuli that signal through the MAP kinase pathway.
SIZE: 118 amino acids; 12580 Da
SUBUNIT: Nonphosphorylated EIF4EBP1 competes with EIF4G1/EIF4G3 to interact with EIF4E; insulin stimulated MAP-kinase (MAPK1 and MAPK3) phosphorylation of EIF4EBP1 causes dissociation of the complex allowing EIF4G1/EIF4G3 to bind and consequent initiation of translation. Rapamycin can attenuate insulin stimulation, mediated by FKBPs.
PTM: Phosphorylated on serine and threon -20°C

05-621   ANTI-CUG-BP1, PC 12-309   品牌 Millipore

ANTI-CUG-BP1, PC 12-309

货号： 05-621      产品名称： ANTI-CUG-BP1, PC 12-309   品牌： Millipore 规格： EA 3周到货 生化实验

Anti-CUGBP1 Antibody, clone 3B1
Close
REFERENCES
HTS-Compatible Patient-Derived Cell-Based Assay to Identify Small Molecule Modulators of Aberrant Splicing in Myotonic Dystrophy Type 1.
Debra A O’Leary,Leonardo Vargas,Orzala Sharif,Michael E Garcia,Yury J Sigal,Siu-Kei Chow,Christian Schmedt,Jeremy S Caldwell,Achim Brinker,Ingo H Engels (2010) Current chemical genomics.4
Identification of a (CUG)n triplet repeat RNA-binding protein and its expression in myotonic dystrophy.
Timchenko, L T, et al. (1996) Nucleic Acids Res., 24: 4407-14 (1996)
Species Reactivity Key Applications Host Format Antibody Type
B, H, M, Po, R, Rb  EMSA, IP, WB, ICC Mouse Purified Monoclonal Antibody
Description:
Anti-CUGBP1 Antibody, clone 3B1
Promotional Text:
Special Shipping Offer on Antibodies
100% Performance Guaranteed
Trade Name:
Upstate (Millipore)
Specificity:
CUG-BP1
Molecular Weight:
50kDa
Immunogen:
Full-length GST fusion protein corresponding to human CUG-BP1, also known as heterogeneous nuclear ribonucleoprotein (hnRNP) hNab50
Isotype:
IgG
Background Information:
Myotonic dystrophy (MD) is an autosomal dominant neuromuscular disease that is associated with a (CTG)n repeat expansion in the 3′-untranslated region of the myotonin protein kinase (Mt-PK) gene. A (CUG)n oligonucleotides triplet repeat pre-mRNA/mRNA binding protein may play an important role in DM pathogenesis. HeLa cell protein, CUG-BP1, has been purified based upon its ability to bind specifically to (CUG)8 oligonucleotides in vitro. CUG-BP1 is the major (CUG)8 binding activity in normal cells. CUG-BP1 has been identified as isoforms of a novel heterogeneous nuclear ribonucleoprotein (hnRNP), hNab50. The CUG-BP/hNab50 protein is localized predominantly in the nucleus and is associated with polyadenylated RNAs in vivo. In vitro RNA-binding/photocrosslinking studies demonstrate that CUG-BP/hNab50 binds to RNAs containing the Mt-PK 3′-UTR.
View All »
Species Reactivity:
Bovine
Human
Mouse
Pig
Rat
Rabbit
View All »
Control:
HeLa lysates
Quality Assurance:
routinely evaluated by immunoblot on HeLa nuclear extract
Purification Method:
Protein G chromatography
Presentation:
0.1M Tris-glycine, pH 7.4, 0.15M NaCl, 0.05% sodium azide before the addition of glycerol to 30%
Storage Conditions:
2 years at -20°C
UniProt Number:
Q92879
Entrez Gene Number:
NM_006560 and others
Gene Symbol:
CUGBP1
BRUNOL2
CELF-1
CUGBP
NAB50
CELF1
CUG-BP
hNab50
CUG-BP1
View All »
Usage Statement:
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
View All »
Key Applications:
Electrophoretic Mobility Shift Assay
Immunoprecipitation
Western Blotting
Immunocytochemistry
Entrez Gene Summary:
Members of the CELF/BRUNOL protein family contain two N-terminal RNA recognition motif (RRM) domains, one C-terminal RRM domain, and a divergent segment of 160-230 aa between the second and third RRM domains. Members of this protein family regulate pre-mRNA alternative splicing and may also be involved in mRNA editing, and translation. This gene may play a role in myotonic dystrophy type 1 (DM1) via interactions with the dystrophia myotonica-protein kinase -20°C

05-725   ANTI-53BP1, CLONE BP18   品牌 Millipore

ANTI-53BP1, CLONE BP18

货号： 05-725      产品名称： ANTI-53BP1, CLONE BP18   品牌： Millipore 规格： EA 3周到货 生化实验

Anti-53BP1 Antibody, clone BP18
Close
REFERENCES
p53 Binding protein 53BP1 is required for DNA damage responses and tumor suppression in mice.
Ward, Irene M, et al. (2003) Mol. Cell. Biol., 23: 2556-63 (2003)
Tumor suppressor p53 binding protein 1 (53BP1) is involved in DNA damage-signaling pathways.
Rappold, I, et al. (2001) J. Cell Biol., 153: 613-20 (2001)
Species Reactivity Key Applications Host Format Antibody Type
H, M  IP, WB Mouse Ascites Monoclonal Antibody
Description:
Anti-53BP1 Antibody, clone BP18
Promotional Text:
Special Shipping Offer on Antibodies
100% Performance Guaranteed
Trade Name:
Upstate (Millipore)
Specificity:
53BP1
Molecular Weight:
~250kDa
Immunogen:
Mix of three GST fusion proteins corresponding to residues 1-337, 338-671, and 1331-1664, respectively, of human 53BP1
Isotype:
IgM
Species Reactivity:
Human
Mouse
Quality Assurance:
routinely evaluated by immunoblot on whole cell lysates from HeLa cells
Purification Method:
Ascites
Presentation:
mouse ascites IgM containing 0.05% sodium azide and 30% glycerol
Storage Conditions:
2 years at -20°C
UniProt Number:
Q12888
Entrez Gene Number:
NM_005657.1
Gene Symbol:
TP53BP1
53BP1
p53BP1
p202
FLJ41424
MGC138366
View All »
Usage Statement:
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
View All »
Key Applications:
Immunoprecipitation
Western Blotting
UniProt Summary:
FUNCTION: SwissProt: Q12888 # May have a role in checkpoint signaling during mitosis (By similarity). Enhances TP53-mediated transcriptional activation. Binds to sites of DNA damage. Plays a role in the response to DNA damage.
SIZE: 1972 amino acids; 213574 Da
SUBUNIT: Interacts with IFI202A (By similarity). Binds to the central domain of TP53/p53. May form homo-oligomers. Interacts with DCLRE1C. Interacts with histone H2AFX and this requires phosphorylation of H2AFX on ‘Ser-139’. Interacts with histone H3 that has been methylated on ‘Lys-79’. Does not bind unmethylated histone H3.
SUBCELLULAR LOCATION: Nucleus. Kinetochore. Note=Associated with kinetochores. Both nuclear and cytoplasmic in some cells. Recruited to sites of DNA damage, such as double stand breaks.
PTM: Asymmetrically dimethylated on Arg residues by PRMT1. Methylation is required for DNA binding. & Phosphorylated in an ATM-dependent manner in response to DNA damage.
SIMILARITY: SwissProt: Q12888 ## Contains 2 BRCT domains.
View All »
Brand Family:
Upstate
Product Name:
Anti-53BP1 Antibody, clone BP18
Antibody Type:
Monoclonal Antibody
Qty/Pk:
200 µL
Format:
Ascites
Host:
Mouse
Applications:
Detect 53BP1 with Anti-53BP1 Antibody, clone BP18 (Mouse Monoclonal Antibody), that has been shown to work in IP & WB.

-20°C

05-726   ANTI-53BP1, CLONE BP13   品牌 Millipore

ANTI-53BP1, CLONE BP13

货号： 05-726      产品名称： ANTI-53BP1, CLONE BP13   品牌： Millipore 规格： EA 3周到货 生化实验

Anti-53BP1 Antibody, clone BP13
Close
REFERENCES
Spatial dynamics of DNA damage response protein foci along the ion trajectory of high-LET particles.
Guanghua Du,Guido A Drexler,Werner Friedland,Christoph Greubel,Volker Hable,Reiner Krücken,Alexandra Kugler,Laura Tonelli,Anna A Friedl,Günther Dollinger (2011) Radiation research.176
Quantitative analysis of DNA-damage response factors after sequential ion microirradiation.
Christoph Greubel, Volker Hable, Guido A Drexler, Andreas Hauptner, Steffen Dietzel, Hilmar Strickfaden, Iris Baur, Reiner Krücken, Thomas Cremer, Anna A Friedl, Günther Dollinger, Christoph Greubel, Volker Hable, Guido A Drexler, Andreas Hauptner, Steffen Dietzel, Hilmar Strickfaden, Iris Baur, Reiner Krücken, Thomas Cremer, Anna A Friedl, Günther Dollinger, Christoph Greubel, Volker Hable, Guido A Drexler, Andreas Hauptner, Steffen Dietzel, Hilmar Strickfaden, Iris Baur, Reiner Krücken, Thomas Cremer, Anna A Friedl, Günther Dollinger (2008) Radiation and environmental biophysics.47:415-22
Telomere dysfunction in human keratinocytes elicits senescence and a novel transcription profile.
Fay Minty, Johanna K Thurlow, Paul R Harrison, E Kenneth Parkinson (2008) Experimental cell research.314:2434-47
Histone H2AX is phosphorylated in an ATR-dependent manner in response to replicational stress.
Ward, I M and Chen, J (2001) J. Biol. Chem., 276: 47759-62 (2001)
View All
Species Reactivity Key Applications Host Format Antibody Type
H  IP, WB, ICC Mouse Purified Monoclonal Antibody
Description:
Anti-53BP1 Antibody, clone BP13
Promotional Text:
Special Shipping Offer on Antibodies
100% Performance Guaranteed
Trade Name:
Upstate (Millipore)
Specificity:
53BP1
Molecular Weight:
~250kDa
Immunogen:
Mix of three GST fusion proteins corresponding to residues 1-337, 338-671, and 1331-1664, respectively, of human 53BP1
Isotype:
IgG1
Species Reactivity:
Human
Application Notes:
Does not cross-react with mouse.
Quality Assurance:
routinely evaluated by immunoblot on lysates from HeLa cells
Purification Method:
Protein G purfied
Presentation:
0.1M Tris-glycine, pH 7.4, 0.15M NaCl, 0.05% sodium azide before the addition of glycerol to 30%
Storage Conditions:
2 years at -20°C
UniProt Number:
Q12888
Entrez Gene Number:
NM_005657.1
Gene Symbol:
TP53BP1
53BP1
p53BP1
p202
FLJ41424
MGC138366
View All »
Usage Statement:
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
View All »
Key Applications:
Immunoprecipitation
Western Blotting
Immunocytochemistry
UniProt Summary:
FUNCTION: SwissProt: Q12888 # May have a role in checkpoint signaling during mitosis (By similarity). Enhances TP53-mediated transcriptional activation. Binds to sites of DNA damage. Plays a role in the response to DNA damage.
SIZE: 1972 amino acids; 213574 Da
SUBUNIT: Interacts with IFI202A (By similarity). Binds to the central domain of TP53/p53. May form homo-oligomers. Interacts with DCLRE1C. Interacts with histone H2AFX and this requires phosphorylation of H2AFX on ‘Ser-139’. Interacts with histone H3 that has been methylated on ‘Lys-79’. Does not bind unmethylated histone H3.
SUBCELLULAR LOCATION: -20°C