Anti-hnRNP L, clone 4D11
货号： 05-1518 产品名称： Anti-hnRNP L, clone 4D11 品牌： Millipore 规格： *** 3周到货 生化实验
Anti-hnRNP L, clone 4D11
Species Reactivity Key Applications Host Format Antibody Type
H WB, IP Mouse Purified Monoclonal Antibody
Description:
Anti-hnRNP L Antibody, clone 4D11
Promotional Text:
Special Shipping Offer on Antibodies
100% Performance Guaranteed
Molecular Weight:
~64 kDa observed
Immunogen:
Human hnRNP proteins (from HeLa cells) purified by affinity chromatography on ssDNA agarose.
Clone:
4D11
Isotype:
IgG1κ
Background Information:
Heterogeneous nuclear ribonucleoprotein L (HnRNP-L) is a polypyrimidine tract-binding protein (PTB)-binding protein and is commonly found in the nucleoplasm. HnRNP-L appears to be involved in transcription, pre-mRNA processing, mature mRNA transport to the cytoplasm, and translation. HnRNP-L binds to an RNA sequence element in a very sequence-specific manner that permit intron-independent gene expression.
View All »
Species Reactivity:
Human
Species Reactivity Note:
Demonstrated to react with human.
Application Notes:
Immunoprecipitation:
A representative lot detected hnRNP L in IP. (Topp, J.D., et al. (2008). RNA. 14:2038–2049.)
Control:
HepG2 cell lysate
Quality Assurance:
Evaluated by Western Blot in HepG2 cell lysate.
Western Blot Analysis: 0.01 µg/mL of this antibody detected hnRNP L on 10 µg of HepG2 cell lysate.
Purification Method:
Protein G
Presentation:
Purified mouse monoclonal IgG1κ in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Storage Conditions:
Stable for 1 year at 2-8°C from date of receipt.
UniProt Number:
P14866
Entrez Gene Number:
NP_001005335
Gene Symbol:
HNRNPL
HNRPL
hnRNP L
Alternate Names:
heterogeneous nuclear ribonucleoprotein L
Usage Statement:
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
View All »
Key Applications:
Western Blotting
Immunoprecipitation
Entrez Gene Summary:
Heterogeneous nuclear RNAs (hnRNAs) which include mRNA precursors and mature mRNAs are associated with specific proteins to form heterogenous ribonucleoprotein (hnRNP) complexes. Heterogeneous nuclear ribonucleoprotein L is among the proteins that are stably associated with hnRNP complexes and along with other hnRNP proteins is likely to play a major role in the formation, packaging, processing, and function of mRNA. Heterogeneous nuclear ribonucleoprotein L is present in the nucleoplasm as part of the HNRP complex. HNRP proteins have also been identified outside of the nucleoplasm. Exchange of hnRNP for mRNA-binding proteins accompanies transport of mRNA from the nucleus to the cytoplasm. Since HNRP proteins have been shown to shuttle between the nucleus and the cytoplasm, it is possible that they also have cytoplasmic functions. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq].
View All »
UniProt Summary:
FUNCTION: This protein is a component of the heterogeneous nuclear ribonucleoprotein (hnRNP) complexes which provide the substrate for the processing events that pre-mRNAs undergo before becoming functional, translatable mRNAs in the cytoplasm. L is associated with most nascent transcripts including those of the landmark giant loops of amphibian lampbrush chromosomes.