Anti-HIF-1 a, clone EP1215Y

货号： 04-1006      产品名称： Anti-HIF-1 a, clone EP1215Y   品牌： Millipore 规格： EA 3周到货 生化实验

Anti-HIF-1α, clone EP1215Y, Rabbit Monoclonal Antibody (04-1006)
Species Reactivity Key Applications Host Format Antibody Type
H, R  WB, ICC, IH(P) Rabbit Unpurified Monoclonal Antibody
Description:
Anti-HIF-1α Antibody, clone EP1215Y, rabbit monoclonal
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Specificity:
This antibody recognizes HIF-1α at and around the C-terminus.
Molecular Weight:
~120 kDa
Epitope:
C-terminus
Immunogen:
Synthetic peptide corresponding to residues near the C-terminus of human HIF-1α.
Clone:
EP1215Y
Isotype:
IgG
Background Information:
Hypoxia, a condition of low tissue O2 concentration, plays an important role in normal physiological processes and tumor formation. Under hypoxic conditions mammalian cells up regulate the expression of hypoxic genes, including induction of angiogenesis and a switch to anaerobic metabolism, in order to survive. HIF-1 (Hypoxia Inducible Factor-1) is one of the key regulators of the transcriptional response to oxygen deprivation (1). HIF-1 is composed of two subunits, HIF-1αand HIF-1β also known as aryl hydrocarbon receptor nuclear translocator (ARNT)) that are members of the basic helix-loop-helix (bHLH) Per-Arnt-Sim (PAS) (bHLH-PAS) family of transcription factors. HIF-1 is essential for angiogenesis, embryonic development, and is associated with tumor progression, erythropoiesis, vascular development/remodeling, vasodilation, and glucose/energy metabolism. The over expression of HIF-1αhas been demonstrated in many common human cancers including prostate and breast, in which HIF-1α levels are associated with increase vascularitry and tumor progression. Besides physiological hypoxia, genetic abnormalities frequently detected in human cancers, such as loss of function mutations (Von Hippel-Lindau, p53, and PTEN), are associated with induction of HIF1 activity and expression of HIF-1-inducible genes (1).
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Species Reactivity:
Human
Rat
Species Reactivity Note:
Human and rat.
Application Notes:
Immunohistochemistry Analysis: A 1:100-250 dilution from a previous lot detected HIF-1α in human liver carcinoma tissue.
Immunocytochemistry Analysis: A 1:100-250 dilution from a previous lot was used in IC.
Control:
Ramos + CoCl2 lysate
Quality Assurance:
Evaluated by Western Blot on Ramos + CoCl2 lysates.
Western Blot Analysis: A 1:2,000 dilution of this antibody was used to detect HIF-1α in Ramos + CoCl2 lysate.
Purification Method:
Unpurified
Presentation:
Rabbit Monoclonal in buffer containing 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl containing 40% Glycerol, 0.01% sodium azide and 0.05% BSA.
Storage Conditions:
Stable for 1 year at -20ºC from date of receipt. Handling Recommendations: Upon first thaw, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance. Note: Variability in freezer temperatures below -20°C may cause glycerol containing solutions to become frozen during storage.
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UniProt Number:
Q16665
Entrez Gene Number:
NM_001530.2
Gene Symbol:
HIF-1alpha
HIF1
HIF1-ALPHA
MOP1
PASD8
bHLHe78
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Alternate Names:
ARNT interacting protein
ARNT-interacting protein
Basic-helix-loop-helix-PAS protein MOP1
HIF-1 alpha
HIF1 alpha
Member of PAS protein 1
hypoxia inducible factor 1, alpha subunit (basic