Anti-hnRNP Q, clone 18E4

货号： 05-1517      产品名称： Anti-hnRNP Q, clone 18E4   品牌： Millipore 规格： EA 3周到货 生化实验

Anti-hnRNP Q, clone 18E4 Antibody (05-1517)
Species Reactivity Key Applications Host Format Antibody Type
H  WB, ICC, IP Mouse Purified Monoclonal Antibody
Description:
Anti-hnRNP Q Antibody, clone 18E4
Promotional Text:
Special Shipping Offer on Antibodies
100% Performance Guaranteed
Specificity:
This antibody recognizes hnRNP Q.
Molecular Weight:
The calculated molecular weight is 70 kDa. Clone 18E4 can recognizes four bands on the Western Blot of total HeLa cell lysate corresponding to molecular masses of ~80, ~70, ~60 and ~55 kDa. hnRNP Q has three isoforms of ~70– 55 kDa. The ~80 kDa reactive band was identified as hnRNP R, which migrates at ~80 kDa on SDS–PAGE. (Mourelatos., Z., et al., EMBO J., 20, 5443-5452 (2001).)
View All »
Epitope:
Unknown
Immunogen:
Recombinant protein corresponding to human hnRNP Q.
Clone:
18E4
Background Information:
Heterogeneous nuclear ribonucleoprotein Q commoly refered to as hnRNP Q can be found as three different isoforms derived from alternative splicing of a single gene. This RNA binding protein is implicated in control of mRNA precursor splicing for the survival of motor neurons (SMN) protein, a gene. Loss-of-function mutations of SMN are linked to spinal muscular atrophy (SMA) a common neurodegenerative disease. It has been shown that hnRNP Q proteins interact with SMN, and this is interacton is required for efficient pre-mRNA splicing in vitro. Current data suggests that hnRNP Q is a splicing modulator of SMN.
View All »
Species Reactivity:
Human
Species Reactivity Note:
Demonstrated to react with human.
Application Notes:
Immunocytochemistry
Cited by independent researcher using a representative lot.
Immunoprecipitation
Cited by independent researcher using a representative lot.
Control:
HeLa cell lysate
Quality Assurance:
Evaluated by Western Blot in HeLa cell lysate.
Western Blot Analysis: 1 µg/ml of this antibody detected hnRNP Q on 10 µg of HeLa cell lysate.
Purification Method:
Protein G purfied
Presentation:
Purified mouse monoclonal IgGκ in buffer containing 0.1 M Tris-Glycine (pH 7.4, 150 mM NaCl) with 0.05% sodium azide.
Storage Conditions:
Stable for 1 year at 2-8°C from date of receipt.
UniProt Number:
O60506
Entrez Gene Number:
NP_001153145.1
Gene Symbol:
hnRNP-Q
hnRNP Q
SYNCRIP
HNRPQ
NSAP1
View All »
Alternate Names:
heterogeneous nuclear ribonucleoprotein Q-like
Synaptotagmin-binding, cytoplasmic RNA-interacting protein
Glycine- and tyrosine-rich RNA-binding protein
GRY-RBP
NS1-associated protein 1
View All »
Usage Statement:
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
View All »
Key Applications:
Western Blotting
Immunocytochemistry
Immunoprecipitation
UniProt Summary:
FUNCTION: Heterogenous nuclear ribonucleoprotein (hnRNP) implicated in mRNA processing mechanisms. Component of the CRD-mediated complex that promotes MYC mRNA stability. Isoform 1, isoform 2 and isoform 3 are associated in vitro with pre-mRNA, splicing intermediates and mature mRNA protein complexes. Isoform 1 binds to apoB mRNA AU-rich sequences. Isoform 1 is part of the APOB mRNA editosome c 2°C – 8°C