ANTI-RAC1, CLONE 23A8 250UG

货号： 05-389      产品名称： ANTI-RAC1, CLONE 23A8 250UG   品牌： Millipore 规格： EA 3周到货 生化实验

Anti-Rac1 Antibody, clone 23A8
Species Reactivity Key Applications Host Format Antibody Type
H, M, R  IHC, IP, WB Mouse Purified Monoclonal Antibody
Description:
Anti-Rac1 Antibody, clone 23A8
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Trade Name:
Upstate (Millipore)
Specificity:
Recognizes Rac1; Strong Rac1 preference with slight Rac2 cross-reactivity.
Molecular Weight:
21 kDa
Immunogen:
Recombinant protein containing the full length human Rac
Clone:
23A8
Isotype:
IgG2b
Background Information:
Rac1 is a member of a RhoGTPase subfamily (Rac1-Rac4) that transduces extracellular signals from G-coupled protein receptors (GPCR), integrins and growth factor receptors to effector molecules that modulate multiple signaling pathways. While Rac1 supports the activation of MAPkinase pathways and gene activation, it’s most important functions include the regulation of cytoskeletal structures. Together with Rho (regulator of stress fibers) and Cdc42 (regulator of filopodia), Rac modulates the formation of focal adhesion (FA) complexes; membrane ruffles and lamellipodia that contribute to important cell functions related to cell attachment and movement.
Species Reactivity:
Human
Mouse
Rat
Control:
Immunoblot/Immunoprecipitation: Rat brain microsomal protein preparation
Immunohistochemistry: Rat brain sections
Quality Assurance:
routinely evaluated by immunoblot on rat brain microsomal protein preparation
Purification Method:
Protein G purfied
Presentation:
Protein G Purified immunoglobulin in Protein G Purified immunoglobulin in 0.1M Tris-glycine, pH 7.4, 0.15M NaCl, 0.05% sodium azide before the addition of glycerol to 30%
Storage Conditions:
Maintain for 2 years at -20°C from date of shipment. Aliquot to avoid repeated freezing and thawing. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
UniProt Number:
P63000
Entrez Gene Number:
NM_006908
Gene Symbol:
RAC1
MGC111543
ORFNames=MIG5
MIG5
TC-25
p21-Rac1
Usage Statement:
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Key Applications:
Immunohistochemistry
Immunoprecipitation
Western Blotting
Entrez Gene Summary:
The protein encoded by this gene is a GTPase which belongs to the RAS superfamily of small GTP-binding proteins. Members of this superfamily appear to regulate a diverse array of cellular events, including the control of cell growth, cytoskeletal reorganization, and the activation of protein kinases. Several alternatively spliced transcript variants of this gene have been described, but the full-length nature of some of these variants has not been determined.
UniProt Summary:
FUNCTION: SwissProt: P63000 # Isoform B has an accelerated GEF-independent GDP/GTP exchange and an impaired GTP hydrolysis, which is restored partially by GTPase-activating proteins. It is able to bind to the GTPase-binding domain of PAK but not full-length PAK in a GTP- dependent manner, suggesting that the insertion does not completely abolish effector interaction.
SIZE: 192 amino acids; 21450 Da
SUBUNIT: Interacts with the GEF proteins PREX1, RASGRF2, DOCK1, DOCK2 and DOCK7, which promote the exchange between GDP and GTP -20°C