IRAK1 Antibody (C-term)

货号： 07-1601      产品名称： IRAK1 Antibody (C-term)   品牌： Millipore 规格： EA 三周到货 生化实验

Anti-IRAK1, C-terminus
Species Reactivity Key Applications Host Format Antibody Type
H  WB Rabbit Purified Polyclonal Antibody
Description:
Anti-IRAK1 Antibody, CT
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Specificity:
This antibody recognizes the C-terminal region of IRAK1.
Molecular Weight:
~77 kDa
Epitope:
C-terminus
Immunogen:
KLH-conjugated synthetic linear peptide corresponding to the C-terminal region of IRAK1.
Background Information:
IRAK1, also known as Interleukin-1 receptor associated kinase 1, belongs to the tyrosine kinase related (TKL) family and is involved with mRNA stabilization and activation of NF-kappa B. IRAK1 is located in all tissue as 2 isoforms, with 1 binding quickly and then degrading while isoform 2 is activated by kinase, resulting in a slower more sustained response to cytokines. Phosphorylation of IRAK1 leads to recruitment of the adapter protein PELI1. IRAK1 utilizes 2 pathways, the first one allows for mediation of IL-1 inflammatory responses when TRAF6 is bound, which activates NF-kappa B, thereby inhibiting I kappa B alpha. A second pathway does not involve TRAF6 and results in the stabilization of mRNA.
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Species Reactivity:
Human
Species Reactivity Note:
Human.
Control:
Jurkat lysate
Quality Assurance:
Evaluated by western blot in jurkat lysate.
Western Blot Analysis: A 1:50-1:500 dilution of this antibody detected IRAK1 on jurkat lysate.
Purification Method:
Protein G purfied
Presentation:
Purified rabbit polyclonal in buffer containing PBS and 0.09% sodium azide.
Storage Conditions:
Stable for 6 months at 2-8ºC from date of receipt. For long term storage, store at -20ºC.
Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
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UniProt Number:
P51617
Entrez Gene Number:
NP_001020413.1
Gene Symbol:
IRAK
IRAK-1
pelle
EC 2.7.11.1
Alternate Names:
interleukin-1 receptor-associated kinase 1
Pelle homolog
Usage Statement:
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Key Applications:
Western Blotting
Entrez Gene Summary:
This gene encodes the interleukin-1 receptor-associated kinase 1, one of two putative serine/threonine kinases that become associated with the interleukin-1 receptor (IL1R) upon stimulation. This gene is partially responsible for IL1-induced upregulation of the transcription factor NF-kappa B. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq]
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UniProt Summary:
Function: Binds to the IL-1 type I receptor following IL-1 engagement, triggering intracellular signaling cascades leading to transcriptional up-regulation and mRNA stabilization. Isoform 1 binds rapidly but is then degraded allowing isoform 2 to mediate a slower, more sustained response to the cytokine. Isoform 2 is inactive suggesting that the kinase activity of this enzyme is not required for IL-1 signaling. Once phosphorylated, IRAK1 recruits the adapter protei 2-8℃