Anti-PGF
货号： ABC375 产品名称： Anti-PGF 品牌： Millipore 规格： EA 三周到货 生化实验
Anti-PGF
Species Reactivity Key Applications Host Format Antibody Type
H WB, IHC Rabbit Affinity Purified Polyclonal Antibody
Description:
Anti-PGF Antibody
Molecular Weight:
~28 kDa observed
Immunogen:
GST-tagged fusion protein corresponding to human PGF.
Background Information:
Placenta growth factor (PGF, PIGF, PGFL, or PLGF) is a secreted protein primarily produced by placental trophoblast tissues but also expressed in other endothelial tissues and cancers. There are three isoforms, PGF-1, PGF-2, and PGF-3 (PIGF-1, PIGF-2, PIGF-3). PGF-2 is expressed up until week 8 in the placenta; PGF-1 and PGF-3 are continuously expressed by the placental tissues but only PGF-1 is found in colon and mammary carcinomas. PGF acts to stimulate angiogenesis, endothelial growth and migration. PGF is a powerful promoter of tumor growth and is upregulated in some cancers, and PGF is thought to aid in atherosclerotic lesions and neovascular growth surrounding the lesion. Also, PGF appears to aid aldosterone mediated atherosclerosis. Serum levels of PGF can be measured and in some cases used as a potential biomarker for disease or genetic defect. Recent research indicates that PGF levels are lower in mothers with Down syndrome fetuses, and when combined with additional tests PGF data helps lower the false positive detection rate in blood tests. EMD-Millipore’s Anti-PGF rabbit polyclonal antibody has been tested in western blots against MCF7 cell lysates and paraffin embedded immunohistochemistry on human Meningioma tissue sections.
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Species Reactivity:
Human
Application Notes:
Immunohistochemistry Analysis: A 1:50 dilution from a representative lot detected PGF in human meningioma tissue.
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Quality Assurance:
Evaluated by Western Blotting in MCF7 cell lysate.
Western Blotting Analysis: A 1:600 dilution of this antibody detected PGF in MCF7 cell lysate.
Purification Method:
Antigen Affinity purified
Presentation:
Purified rabbit polyclonal in buffer containing PBS with up to 0.1% sodium azide and 50% glycerol.
Storage Conditions:
Stable for 1 year at -20°C from date of receipt.
Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
Note: Variability in freezer temperatures below -20°C may cause glycerol containing solutions to become frozen during storage.
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UniProt Number:
P49763
Gene Symbol:
PGF
PGFL
PLGF
Alternate Names:
Placenta growth factor
PlGF
Usage Statement:
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Key Applications:
Western Blotting
Immunohistochemistry
Product Name:
Anti-PGF
Concentration:
Please refer to the Certificate of Analysis for the lot-specific concentration.
Antibody Type:
Polyclonal Antibody
Qty/Pk:
150 µL
Format:
Affinity Purified
Host:
Rabbit
Applications:
Detect Placenta growth factor using this rabbit polyclonal antibody, Anti-PGF Antibody validated for use in western blotting & IHC.
-20℃
